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In the absence of Mud2, Sub2 function becomes dispensable. Paper-8690202.
In addition we observed that BBP and Mud2 are not detectable in pre-spliceosomes. Paper-1882245.
We propose that Sub2 functionally interacts with Mud2 both before and after PS formation. Paper-8690202.
Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase. Paper-8690202.
The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Paper-135900.
Mutational analyses of trimethylguanosine synthase ( Tgs1) and Mud2: proteins implicated in pre-mRNA splicing. Paper-15250401.
Intriguingly, Prp40p and Mud2p also bind the phylogenetically conserved branchpoint binding protein (BBP/ SF1). Paper-1951204.
These observations support a model in which BBP and Mud2 are recycled during transition from CC2 to pre-spliceosome. Paper-1882245.
We have now analyzed the presence of BBP and Mud2 in yeast splicing complexes using supershift and coprecipitation assays. Paper-1882245.
Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly. Paper-1882245.
Genetic and biochemical criteria indicate a direct interaction between Msl5p and both Mud2p and the U1 snRNP protein Prp40p. Paper-1013577.
We found that BBP is present together with Mud2 in commitment complex 2 ( CC2), but is not detectable in commitment complex 1 ( CC1). Paper-1882245.
The requirements for Mud2 function are apparently more stringent when yeast cells lack TMG caps than when they lack Mud1 or Swm2. Paper-15250401.
Moreover, experiments in the two-hybrid system show that PRP11P, a recently identified component of U2 snRNP, can interact directly with MUD2P. Paper-135900.
Using mutants of MUD2 and BBP, we show that the BBP- Mud2p complex bridges partner-specific Prp39p, Mer1p, Clf1p and Smy2p two-hybrid interactions. Paper-14316832.
Thirdly, Mer1p interacts with the branch point binding proteins Mud2p and Bbp1p and the U2 snRNP protein Prp11p by two-hybrid assays. Paper-10410326.
Moreover, deletion of MUD2 exacerbates the cs phenotype of sub2 alleles yet suppresses both the ts sub2-1 and the lethal Deltasub2 phenotypes. Paper-8690202.
We tested the predictions that the yeast UAP56 homolog, SUB2, is required for the same step and functions collaboratively with MUD2, the yeast homolog of U2AF65. Paper-8690202.
Here we exploited the synthetic lethal interactions of tgs1Delta and mud2Delta mutations to identify essential structural features of the Tgs1 and Mud2 proteins. Paper-15250401.
Interestingly, depletion of BBP or disruption of MUD2 had no significant effect on pre-spliceosome formation and splicing in vitro but led to a transient accumulation of CC1. Paper-1882245.
Yeast two-hybrid analyses and in vitro binding studies show that Clf1p interacts specifically and differentially with the U1 snRNP- Prp40p protein and the yeast U2AF65 homolog, Mud2p. Paper-1951204.
We propose that a BBP- Mud2p heterodimer binds as a unit to the branchpoint in vivo and serves as a target for the Sub2p-DExD/ H-box ATPase and for other splicing factors during spliceosome assembly. Paper-14316832.
The identified genes encode the Mud2, Ntc20, Prp16, Prp17, Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing ( Bud13, Cet1, Cwc2, and Rds3). Paper-9942762.
A directed two-hybrid screen reveals a network of prospective interactions that includes previously unreported intra-SF3b contacts and SF3b interactions with the RES subunit Bud13p, the Prp5p DExD/ H-box protein, Mud2p, and the late-acting nineteen complex. Paper-11497386.
Other changes in Mud2 elicited distinct phenotypes in tgs1Delta versus mud1Delta backgrounds. mud2Delta also caused a severe growth defect in cells lacking the Tgs1-binding protein encoded by the nonessential gene YNR004w (now renamed SWM2, synthetic with mud2Delta). Paper-15250401.
Accumulation of the U1, U2, and U5 small nuclear ribonucleoprotein particles (snRNPs) and the 3' splice site binding factors Mud2p and BBP was detected in patterns indicative of progressive and complete spliceosome assembly; recruitment of the nineteen complex (NTC) component Prp19p suggests that splicing catalysis is also cotranscriptional. Paper-11005679.
These accession numbers are used for gene MUD2: .
MUD2 is a homologue of KLLA0F26433g (hypothetical protein) from Kluyveromyces lactis NRRL Y-1140.
MUD2 is a homologue of AGOS_ADR130W (ADR130Wp) from Ashbya gossypii ATCC 10895.
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iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.