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Technology evaluation: HPV vaccine (quadrivalent), Aventis Pasteur MSD/ CSL. Paper-10428144.
Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA. Paper-10561716.
Mobility results after 6 months were available for 77 patients (43 ABC/34 CSLP). Paper-12575496.
Smooth Muscle alpha-actin is a direct target of Notch/ CSL. Paper-12046857.
Differential binding of lectins IL-2 and CSL to candida albicans and cancer cells. Paper-1403210.
Furthermore, Maml can rescue Delta RAM transcriptional activity on a CSL-dependent promoter. Paper-9207731.
TACE using ACRms in combination with CSL prolongs the survival of patients with unresectable HCC. Paper-7169016.
The probe did not hybridise to DNA from CS3, CFA/III CS6, CS5 CS6, CS6, CS7, or PCFO159 ETEC. Paper-9043710.
Class 1 integrons were detected by PCR amplification using the primers CS5 and CS3. Paper-12470054.
TNF-alpha production increased slightly in BM-plasma and CSL-plasma, but not in CSL-serum. Paper-8024894.
Structures of CSL, Notch and Mastermind proteins: piecing together an active transcription complex. Paper-13118064.
Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. Paper-11324968.
CSL proteins play a central role in Notch signaling and in Epstein-Barr virus-induced immortalization. Paper-1736602.
We conclude that the HFE gene is likely to be located telomeric of D6S105 and close to CS5. Paper-511465.
The rotational diffusion coefficients of the labeled PC and of CSL were also obtained from the ESR spectra. Paper-105730.
However, double CSL- binding sites in mouse Hes7 promoter were not conserved in human HES7 promoter. Paper-13326457.
Comparative cell response to artificial extracellular matrix proteins containing the RGD and CS5 cell-binding domains. Paper-10539299.
These results suggest a model wherein CSL-dependent Notch signaling confers protection against cutaneous SCC. Paper-12162799.
More recently the Kaposi's sarcoma-associated herpesvirus RTA protein has been found to bind CSL. Paper-10484758.
These results suggest that CS1 and CS5 are recognized by the same or overlapping sites on alpha 4 beta 1. Paper-26620.
However, there was not a strict correlation between the sequence requirements for CSL-dependent activation and AP-1 repression. Paper-9156281.
This effect was abrogated in myogenic cells by a dominant-negative form of CSL, an essential DNA-binding component of the Notch pathway. Paper-10049993.
NICD migrates into the nucleus and associates with the nuclear proteins of the RBP-Jkappa family (also known as CSL or CBF1/Su(H)/Lag-1). Paper-9661858.
These sites are contained within the synthetic peptides CS1 and CS5 (residues 1-25 and 90-109 of the IIICS, respectively). Paper-26620.
Although GLI-, CSL-, and HES/HEY-binding sites were not identified, eleven bHLH-binding sites were identified within human HHIP1 promoter. Paper-10838257.
After translocation to the nucleus, the Notch ICD interacts with the DNA- binding protein CSL to activate gene transcription. Paper-11326169.
CSL is a DNA binding transcription factor that regulates transcription of Notch target genes by interacting with coregulators. Paper-11324973.
Patients 1 and 2 (siblings) were homozygous for a large deletion removing four genes of the cluster: hGH-N, hCS-L, hCS-A and hGH-V. Paper-140651.
Colonization factor antigen CFA/IV (PCF8775) of human enterotoxigenic Escherichia coli: nucleotide sequence of the CS5 determinant. Paper-54068.
Kaposi's Sarcoma-Associated Herpesvirus Lytic Switch Protein Stimulates DNA Binding of RBP-Jk/ CSL To Activate the Notch Pathway. Paper-12226778.
We analyzed the capacity of RAM domain mutants to bind endogenous CBF1, to activate CSL-mediated transactivation, and to repress AP-1. Paper-10210477.
Three of the defined hCS-L mRNAs contain an extended open reading frame similar to that present in the functional GH and CS genes. Paper-7960432.
CSL directly binds a conserved cis element in the SMA promoter, and this consensus sequence is required for Notch-mediated SMA induction. Paper-12046857.
CBF1 is a member of the CSL family of DNA binding factors, which mediate either transcriptional repression or transcriptional activation. Paper-1736602.
Folate requirements of the 2-keto-L-gulonic acid-producing strain Ketogulonigenium vulgare LMP P-20356 in L-sorbose/ CSL medium. Paper-10522721.
The acoustic features (F1, F2, F0, and duration) were analyzed using Computerized Speech Laboratory ( CSL 4300B; Kay Elemetrics, Lincoln Park, NJ). Paper-10317103.
CSL- MAML-dependent Notch1 signaling controls T lineage-specific IL-7R{alpha} gene expression in early human thymopoiesis and leukemia. Paper-13716007.
With 30 mg/ml Sandoglobulin alone, IL-1 beta production changed little from control, whilst IL-6 production increased markedly in CSL-serum only. Paper-8024894.
From 5' to 3' these are: GHN, CSL (encoding chorionic somatomammotropin-like gene), CSA, GHV (encoding GH-variant gene) and CSB. Paper-10552312.
The largest complex is approximately 1.5 MDa and contains both endogenous CSL (for CBF1, Suppressor of Hairless, and Lag-1) and Mastermind-Like-1 (Maml). Paper-9207731.
In other words, duplication of the hCS-L gene to produce the hCS-A gene occurred twice, so that hCS-A genes in humans may have independent origins. Paper-4560664.
The requirement for cooperative assembly of the MAML1.ICN.CSL.DNA complex suggests that a primary function of ICN is to render CSL competent for MAML loading. Paper-9660214.
The human growth hormone/human chorionic somatomammotropin ( hGH/ hCS) gene cluster contains five genes: hGH-N, hGH-V, hCS-B, and hCS-L. Paper-585794.
Four patients of the CSLP-group demonstrated surgical hardware complications, whereas no implant complications were observed within the ABC-group (P = 0.0375). Paper-12575496.
The hCS-A and hCS-B genes encoding human chorionic somatomammotropin and the related hCS-L gene are very similar in their coding and flanking sequences. Paper-731543.
Although head-head sites can elicit a Notch response on their own, use of CBS ( CSL binding site) in tail-tail orientation is context-dependent. Paper-11317069.
Moreover, upregulation of PDGFR-beta expression in response to Notch activation critically required the Notch signal integrator CSL. Paper-12841990.
In order to determine the specific needs and interests of Canadian hospital pharmacists, a survey of the CSHP membership was conducted in April of 1987. Paper-6070558.
The CSL family protein CBF1 is a nuclear mediator of Notch signaling and has been predicted to contain an N-terminal nuclear localization signal in exon 4. Paper-9050232.
Interestingly, the physical interaction of Tip60 with Notch1-IC occurs to a more profound degree in the presence of CSL but does not exist in a trimeric complex. Paper-13415571.
Analysis of 14 deletion and alanine substitution mutants revealed a correlation between CBF1 binding, CSL-mediated transactivation, and AP-1 repression. Paper-10210477.
A hydrophobic pocket on BTD is identified as the likely site of Notch interaction with CSL, which has functional implications for the mechanism of Notch signaling. Paper-10561716.
Using the CS5, we achieved a mean platelet multiple of greater than six times baseline, which compares favorably with the multiple produced using dedicated PRP devices. Paper-12305904.
Regulation of alternative splicing in the IIICS region of human fibronectin pre-mRNA encoding cell binding sites CS1 and CS5. Paper-7460346.
Furthermore, COX-2 and MMP-9 expression requires Notch1 mediated recruitment of Suppressor of Hairless ( CSL) and NF-kappaB to respective promoters. Paper-13668827.
This study was conducted to address the issues we had about our use of the Haemonetics Cell Saver 5 ( CS5) to collect PRP during open heart surgery at our institution. Paper-12305904.
It has recently been shown that activated Notch downregulates mini-chromosome-maintenance (MCM) proteins MCM2 and MCM6 by a CSL-dependent mechanism. Paper-12366152.
Two of these (represented by peptides CS1 and CS5) are present in the alternatively spliced IIICS region and lie in separate, independently spliced segments of this region. Paper-8228096.
The deletion not only affects the structural gene for growth hormone ( GH-N) but also alters sequences adjacent to the chorionic somatomammotropin-like ( CS-L) gene. Paper-5433609.
The level of hCS RNA varied from 22-99% of the total hGH/hCS RNA in the neoplastic trophoblast samples, and variable levels of hGH-V and hCS-L RNA were also observed. Paper-8243992.
Recently, a family of mastermind-like (MAML) transcriptional co-activator genes was identified that encode proteins that cooperate with Notch and CSL to activate transcription. Paper-10484754.
The Notch signalling pathway and its crucial cofactor CSL can maintain cells in an undifferentiated state, and have therefore been associated with a growing list of cancers. Paper-11087906.
Patients where divided into two groups, one with pure RS/ CSL with no associated epithelial features and the second with associated ADH, DCIS or invasive cancer. Paper-11467441.
One of the key milestones in the evolution of the treatment of VWD was the development of Haemate P/Humate-P ( CSL Behring) - the first virus-inactivated factor VIII plasma product. Paper-12995464.
Careful analysis of this role has led to a model of signalling downstream of these receptors, via the CSL ( CBF1, Suppressor of Hairless, Lag-1) family of transcription factors. Paper-9466642.
NotchIC, together with the transcriptional coactivator Mastermind, form a ternary complex with CSL that activates transcription from genes that are responsive to Notch signaling. Paper-12923193.
Among the 19 strains expressing CFA/IV, 16 expressed CS5 and CS6 and produced the heat-stable enterotoxin and most were of serotype O128:H21; the remaining 3 strains produced CS6 only. Paper-51192.
The canonical Notch pathway involves proteolytic liberation of the Notch-1 intracellular domain (NIC-1), which activates CSL ( CBF1, Su(H), and Lag-1)-mediated transactivation. Paper-10210477.
These data support the conclusion that we achieved a high platelet multiple with the CS5, and our use of a modified TEG showed that platelet function of the collected PRP was preserved. Paper-12305904.
The SHPS enables schools to set goals based on individual school needs, and incorporate CSHP goals into school improvement plans--a critical factor in sustainability and accountability. Paper-10621494.
Gamma-secretase-like proteolysis at site 3 ( S3), within the transmembrane domain, releases the Notch intracellular domain (NICD) and activates CSL- mediated Notch signaling. Paper-8468298.
Human enterotoxigenic Escherichia coli isolates expressing the colonization factor antigen CFA/IV (previously designated PCF8775) produce plasmid- encoded CS5 fimbriae. Paper-54068.
Notch/ CSL activation induces SMA expression during endothelial-to-mesenchymal transformation, and Notch activation is required for expression of SMA in vascular smooth muscle cells. Paper-12046857.
CSL is required for both repression and activation of Notch target genes, and is the focal point of a transcriptional switch, mediating interactions with transcriptional coregulators. Paper-13118064.
We show here that the mutation of the S4 sequence leads to hypophosphorylation of ICN1; increased NOTCH1 signaling; and the stabilization of complexes containing ICN1, CSL, and MAML1. Paper-12077010.
NICD is associated with CSL/ RBPSUH and Mastermind ( MAML1, MAML2, or MAML3) to activate Notch target genes, such as HES1 and HES5. Paper-12371447.
Specifically, normal term placentas express higher relative levels of hCS-L, lower relative hGH-V levels and a 70-fold lower hGH-V/CS-L mRNA ratio compared to early placentas. Paper-2087509.
The CS5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin alpha 4 beta 1. Inhibition of alpha 4 beta 1 function by RGD peptide homologues. Paper-26620.
Weak colony hybridization, compared to the control strain, was found with ETEC producing CS5 fimbriae with CS6 antigen, CFA/III fimbriae with CS6 antigen, CS7 fimbriae or PCFO159:H4 fimbriae. Paper-46477.
Notch signaling is a conserved pathway of communication between neighboring cells that results in cell fate specification, and CSL is the universal transcriptional effector of Notch signaling. Paper-10561716.
With antisera raised to substance P ( SP) and human calcitonin gene-related peptide ( CGRP), immunoreactive nerves were demonstrated to innervate the CSL and navicular bursa. Paper-253870.
This new CSL-independent Notch/R-Ras pathway provides a molecular mechanism to explain Notch, integrin, and Ras cross-talk during the development of multicellular organisms. Paper-12504489.
Both transcriptional induction of the Notch ligand Jagged1 by TGF-beta and endogenous levels of the Notch effector CSL contribute to p21 induction and epithelial cytostasis. Paper-13129327.
Five strains were examined by dot-blot tests for the colonization factor antigens CFA/I, CS1, CS2, CS3, CS4, CS5, CS6, CS7, PCFO159, PCFO166 and CFA/III. Paper-1560983.
We previously reported that the apical complex and surface-exposed zoite antigens CSL, GP25-200, and P23 are critical in the infection process and are therefore rational targets. Paper-8501576.
Scans of the promoters of these differentially modulated genes identified a multitude of conserved C promoter binding factor (CBF)1/ CSL elements, implicating Notch signaling in their regulation. Paper-13572142.
Roles of Notch and its effector RBPSuh ( CSL), GATA-3, E2A/ HEB and Id proteins, c-Myb, TCF-1, and members of the Runx, Ets, and Ikaros families are critical. Paper-10766660.
Additional ESCC samples were analyzed using different sets of microsatellite markers ( CS1- CS5) within the introns or in close proximity to the 3' end of the CBP gene. Paper-10347943.
In the ninth patient homozygous for a 7.6-kb deletion, the breakpoints are contained within a 29-bp perfect repeat lying 5' to GH1 and the human chorionic somatomammotropin pseudogene ( CSHP1). Paper-5870664.
CONCLUSIONS: The RAM domain and ankyrin repeats are required for Notch signaling and activity, and the CSL pathway is central to the inhibitory effect of Notch on osteoblastogenesis. Paper-12155595.
CFA testing of the 246 ETEC isolates showed that 21 (8%) expressed CFA/I, 3 (1%) exhibited CFA/II, 14 (6%) elaborated CFA/IV, while 7 (3%) expressed PCFO159 and PCFO159 plus CS5. Paper-9044690.
Signaling through the Notch pathway activates the proteolytic release of the Notch intracellular domain (ICD), a dedicated transcriptional coactivator of CSL enhancer-binding proteins. Paper-9211575.
Positive regulation of colonization factor antigen I ( CFA/I) production by enterotoxigenic Escherichia coli producing the colonization factors CS5, CS6, CS7, CS17, PCFO9, PCFO159:H4 and PCFO166. Paper-46477.
Notch pathway activation induces translocation of intracellular Notch (ICN) to the nucleus, where it interacts with the transcription factor CSL ( CBF1/RBP-Jk, Suppressor of Hairless, Lag-1). Paper-10561719.
Although the mechanism of transduction impinges directly on the nucleus to regulate transcription through the CSL [ CBF-1/Su(H)/LAG-2] DNA binding protein, there are few known direct target genes. Paper-13593728.
In the cell line CAKI-2, interleukin 1 beta, Platelet-derived growth factor BB and transforming growth factor beta 1 all increased mRNA splice variants where the CS1 and CS5 regions were removed. Paper-7942199.
The type III connecting segment of fibronectin contains two cell binding sites, represented by the peptides CS1 and CS5, that are recognized by the integrin receptor alpha 4 beta 1. Paper-48501.
We hypothesized that neutralizing monoclonal antibodies (MAbs) targeting the apical complex and surface antigens CSL, GP25-200, and P23 could passively immunize against cryptosporidiosis. Paper-9356838.
The subsequently cleaved intracellular domain of Notch receptors translocates to the nucleus where it interacts with the transcriptional regulator CSL, thereby regulating expression of target genes. Paper-12366152.
In addition, beta-catenin enhanced the transcriptional activity of NICD on the hairy and enhancer of split 1 ( HES1) and CSL through its C-terminal transactivation domain. Paper-13587262.
The baseline ( CBL) surface hydration at 1 second and the rate of change of SEC during probe occlusion ( CSL) were used as measures of surface hydration and transepidermal water movement, respectively. Paper-369468.
MAMLs form a functional DNA-binding complex with the cleaved Notch receptor and the transcription factor CSL, thereby regulating transcriptional events that are specific to the Notch pathway. Paper-12923198.
In peptide inhibition experiments, CS5 was inhibitory for melanoma cell spreading on both CS5-IgG and CS1-IgG conjugates; conversely, CS1 inhibited spreading on both CS1-IgG and CS5-IgG. Paper-26620.
A closely similar rate of positive reactions was encountered among patients with Crohn's disease following tests with batch 0025 of CSL suspension and with another lot (lot 14) derived from spleen K12. Paper-2572967.
Further, although low hCS-L RNA levels (< 1%) were found in term placenta and two of the hydatidiform moles, hCS-L transcripts represented 11% of the total hGH/hCS RNA in a third hydatidiform mole. Paper-8243992.
RESULTS: Notch increased the transactivation of transiently transfected 12xCSL-Luc constructs, containing 12 repeats of an RBP-Jkappa/ CSL binding site, and of the hairy and E (spl) (HES)-1 promoter. Paper-12155595.
Recruitment of Mastermind-like 1 ( MAML1) to CSL.ICN complexes on DNA requires inclusion of the ankyrin repeat domain of ICN, and N- and C-terminal sequences of CSL extending beyond the DNA-binding region. Paper-9660214.
Functionally, Delta4 is indistinguishable from Jagged1 in its abilities to inhibit myogenesis and to stimulate transcription through Notch1 and the DNA binding protein CSL. Paper-10577542.
NICD and Smad3 were shown to interact directly, both in vitro and in cells, in a ligand-dependent manner, and Smad3 could be recruited to CSL-binding sites on DNA in the presence of CSL and NICD. Paper-10049993.
Activation of Notch results in its cleavage and the release of its intracellular domain (NICD), which interacts with the CBF1/RBP-Jkappa, Suppressor of Hairless, Lag-1 ( CSL) family of transcription factors. Paper-12155595.
By electron microscopy, CFA/III, CS4, and CS5 appeared as morphologically distinct rodlike fimbriae: CFA/III was 7 to 8 nm in diameter, CS4 was 6 to 7 nm in diameter, and CS5 was 5 to 6 nm in diameter. Paper-6411283.
To understand how NICD displaces CSL-associated co-repressors, we have quantified the binding of different Notch1 ICD regions to a key interaction domain (the beta trefoil domain, or BTD) of human CSL. Paper-12384855.
Notch(IC) associates with the transcriptional repressor RBP-J (recombination recognition sequence binding protein at the J kappa site), also known as CSL [ CBF1/Su(H)/Lag-1], and converts it to an activator. Paper-12874219.
Integrin activation is dependent on gamma-secretase-mediated intramembranous cleavage of membrane-bound Notch releasing intracellular Notch that activates R-Ras, independent of CSL-transcription. Paper-12504489.
In the present study, a panel of 126 MAbs generated against affinity-purified CSL, GP25-200, and P23 was characterized to identify the most efficacious neutralizing MAb formulation targeting each antigen. Paper-8501576.
Transcriptional activation requires the displacement of corepressors from CSL by the intracellular portion of the receptor Notch (NotchIC) and the recruitment of the coactivator protein Mastermind to the complex. Paper-11324973.
In the current paper, Ramain et al. provide compelling evidence for Notch signalling through a CSL-independent pathway and they demonstrate that the cytoplasmic protein, Deltex, is required for this signal. Paper-9466642.
We show that HsDPH3 is the previously described human diphtheria toxin and Pseudomonas exotoxin A sensitivity required gene 1 and that DPH4 encodes a CSL zinc finger-containing DnaJ-like protein. Paper-10644726.
Signaling initiates proteolytic release of the receptor Notch from the membrane, whereupon the intracellular portion of Notch (NotchIC) localizes to the nucleus and engages the DNA- binding transcription factor CSL. Paper-13118064.
We demonstrate that Notch4/int-3-induced activation of the downstream transcription factor, CSL, is abrogated in cells deficient in presenilins or treated with a pharmacological inhibitor of gamma-secretase/presenilins. Paper-10346116.
We investigated the use of a prothrombin complex concentrate ( PCC; Beriplex P/N, CSL Behring, Marburg, Germany) to treat severe bleeding in a variety of settings: cardiac surgery, warfarin therapy and other surgery. Paper-13062762.
We show that the activity of the hCS-B gene enhancer is mediated by two elements, DF-3 and DF-4, whereas the hCS-L and hCS-A gene enhancers display weaker activity due to mutations in their respective DF-3 sites. Paper-731543.
Pbx1 expression is transcriptionally regulated by Notch3 activation, and Notch3/ CSL protein complex directly binds to the Pbx1 promoter segment harboring the CSL-binding sequence. Paper-13065271.
We report that the expression of these genes, hCS-A, hCS-B, hCS-L, and hGH-V, are coordinately induced during fetal development, increasing between 12 and 20 weeks of gestation and then plateauing through term. Paper-7510716.
Ligand activation of Notch leads to the release of Notch IC (the intracellular receptor domain), which translocates to the nucleus and interacts with the DNA-binding protein CSL to control expression of specific target genes. Paper-13227325.
The second cleavage releases the intracellular domain of Notch, which translocates to the nucleus, where it interacts with the CSL family of transcriptional regulators and forms part of a Notch target gene-activating complex. Paper-12348326.
Several recently determined and exciting structures of CSL, NotchIC, and an active transcription complex composed of CSL, NotchIC and Mastermind have revealed new insights into transcriptional regulation in the Notch pathway. Paper-13118064.
In 5% lactic acid test, negative correlation between changing ratio of TEWL (PTEWL) and clinical score at 5 min ( CS5 min), and negative correlation between changing ratio of capacitance ( PCAP) and CS5 min are registered. Paper-10019365.
While each engineered protein contains identical CS5 cell-binding domain sequences, the lysine residues that serve as cross-linking sites are either (i) within the elastin cassettes or (ii) confined to the ends of the protein. Paper-11633540.
While the functions of the EIIIA and EIIIB domains are still to be established, either end of the IIICS domain contains the cell-binding sites CS1 and CS5 recognised by the integrin VLA-4 which is present on mononuclear cells. Paper-7942199.
A comparison of the promoter sequences of the human, rat, and mouse renin genes has revealed a highly conserved sequence homologous to the DNA recognition sequence for CBF1 ( CSL/RBP-Jkappa/Su(H)/LAG1/ RBPSUH). Paper-10779035.
In children with symptomatic or asymptomatic infections by CFA/I, CS1 plus CS3, CS2 plus CS3, or CS5 plus CS6 strains, a repeat episode of diarrhea or infection by the homologous CF type was uncommon. Paper-13342033.
Activation of R-Ras by Notch may be triggered by a noncanonical CSL ( CBF1 or RBP-Jkappa in vertebrates, Suppressor of Hairless in Drosophila, Lag-1 in Caenorhabditis elegans)-independent pathway. Paper-12739390.
A number of Notch-regulated targets, including genes of the hairy/enhancer-of-split family in organisms ranging from Drosophila to humans, are characterized by paired CSL-binding sites in a characteristic head-to-head arrangement. Paper-13110788.
Also, many term placentas from diabetic pregnancies express lower relative levels of hCS-L mRNA and a much higher hGH-V/CS-L mRNA ratio compared to normal term placenta, resembling more an early placenta pattern of expression. Paper-2087509.
The Notch intracellular domain translocates to the nucleus after proteolytic release upon Notch extracellular engagement, and there it displaces corepressors from DNA-bound CSL and recruits activators of Notch target genes. Paper-10561716.
In vitro, Notch in complex with the DNA- binding protein CBF-1/suppressor of hairless/Lag1 ( CSL) bound the VEGFR-3 promoter and transactivated VEGFR-3 specifically in endothelial cells. Paper-12590370.
In the absence of signaling, CSL [ CBF1, Su(H), Lag-1] regulators repress Notch target genes through interactions with several transcriptional co-repressors that recruit histone deacetylases and other chromatin-modifying enzymes. Paper-12348326.
A further region, partially required for oligodendrocyte expression, lies in the 5' region of the same intron and contains a putative CSL binding site, consistent with a role for Notch signalling in sox10 regulation. Paper-13508201.
Notch receptors control differentiation and contribute to pathologic states such as cancer by interacting directly with a transcription factor called CSL (for CBF-1/Suppressor of Hairless/ Lag-1) to induce expression of target genes. Paper-13110788.
The minimal active sequence within CS5, the tetrapeptide Arg-Glu-Asp-Val (REDV), is somewhat related to the Arg-Gly-Asp-Ser (RGDS) sequence that represents a major active site in the central cell-binding domain (CCBD) of fibronectin. Paper-26620.
A small percentage of hCS transcripts stably retain intron 4 through gestation, the majority derived from the hCS-A gene. hCS-L transcripts undergo two distinct, developmentally stable, splicing pathways between exons 2 and 3. Paper-7510716.
We have found that C-promoter binding factor-1 ( CBF-1), a CSL ( CBF-1, Su(H) and Lag-1)-type transcription factor and key effector of the Notch signaling pathway, is a remarkably potent and specific inhibitor of the HIV-1 LTR promoter. Paper-12640143.
Finally, we have identified a CSL [stands for CBF1, Su(H), and Lag-1] binding site within the human and rat cyclin D1 promoters, suggesting that Notch(ic) proteins activate cyclin D1 transcription through a CSL-dependent pathway. Paper-9037624.
We therefore set out to determine the genome-wide response to Notch activation by analyzing the changes in messenger RNA (mRNA) expression and the sites of CSL occupancy within 30 minutes of activating Notch in Drosophila cells. Paper-13593728.
Partial mastectomy was carried out for both diagnosis and treatment, since it was difficult to discriminate whether RS/ CSL accompanied breast cancer even by US, MG, MRI, aspiration cytology, and spring-loaded core needle biopsy (CNB). Paper-12866020.
The aim of our study was to collect retrospective data on the efficacy and safety of Haemate P ( CSL Behring, Marburg, Germany) in a large cohort of well-characterized VWD patients after the introduction of the guidelines for VWD management in Italy. Paper-13323668.
The five-member human growth hormone (hGH)/chorionic somatomammotropin (hCS) gene cluster encodes the pituitary-specific hGH-N gene and four highly related genes ( hGH-V, hCS-A, hCS-B, and hCS-L) that are expressed only in the placenta. Paper-436684.
Here, we show that Notch directly regulates expression of the mesenchymal and smooth muscle cell marker smooth muscle alpha-actin (SMA) in endothelial and vascular smooth muscle cells via activation of its major effector, CSL. Paper-12046857.
The SHPS provides practitioners a detailed but easy-to-use system that enables schools to create new programs or modify existing programs across all eight components of the CSHP model, as well as administrative support critical to sustainability. Paper-10621494.
In the absence of Notch signalling, CSL represses transcription of Notch target genes, and following activation by Notch, CSL is converted into a transcriptional activator and activates transcription of the same genes. Paper-11087906.
As expected, the RAM domain of Notch interacts with the beta trefoil domain of CSL; however, the C-terminal domain of CSL has an unanticipated central role in the interface formed with the Notch ankyrin repeats and Mastermind. Paper-11324973.
We show that active Notch1 binds to a conserved CSL- binding site in the human IL7R gene promoter and critically regulates IL7R transcription and IL-7R alpha chain (IL-7Ralpha) expression via the CSL- MAML complex. Paper-13716007.
These findings indicate that the Notch requirement during the beta-selection checkpoint in vivo is absolute and independent of the pre-TCR, and it depends on transcriptional activation by Notch via the CSL/ RBP-J- MAML complex. Paper-12252181.
Nuclear ICN binds to a highly conserved DNA-binding transcription factor called CSL (also known as RBP-Jkappa, CBF1, Suppressor of Hairless, and Lag-1) and recruits Mastermind-like transcriptional co-activators to form a transcriptional activation complex. Paper-9660214.
In addition, recent evidence of linkage between CL +/- P and two markers (D4S175 and D4S192) in the region 4q25-4q31.3 raised the possibility that a CSL, with a larger effect than either TGFA or RARA, may reside within this region of the human genome. Paper-391570.
The hGH/hCS locus contains two GH genes and three CS genes spanning 48 kb of DNA in the order: 5'-(hGH-1/ hCS-5/ hCS-1/ hGH-2/hCS-2)-3', confirming analysis of cosmid clones obtained from a different human library (Barsh et al., 1983). Paper-5769498.
When N-terminal cysteine derivatives of the CS peptides were conjugated to IgG by covalent cross-linking with N-succinimidyl-3(2-pyridyldithio)propionate, both the CS1 and CS5 conjugates promoted B16-F10 melanoma cell spreading. Paper-5602321.
The CHA- CSHP pharmaceutical group purchasing program has provided several benefits to participating hospitals and related institutions in Connecticut. The program is run by pharmacists and staffed by individuals knowledgeable in good purchasing techniques. Paper-2667295.
Activation of transmembrane Notch receptors results in proteolytic liberation of the intracellular domain of Notch, which translocates into the nucleus, binds a repressor (C promoter binding factor 1/RBP-Jkappa, Su(H), and Lag-1 ( CSL)), and induces target genes. Paper-9156281.
CFA/I, CS1 to CS5, CS7, CS17, putative CF (PCF) O159 (CS12), PCFO166 (CS14), and CFA/III (CS8) also bound to the rabbit mucus material although with different patterns; the binding of CS2 and CS5 was abolished by meta-periodate treatment. Paper-1344390.
Upon ligand stimulation, a fragment of Notch is released proteolytically and enters the nucleus to form a complex with the DNA- binding protein CSL ( CBF1/Suppressor of Hairless/ Lag1) and activate transcription of Notch-CSL target genes. Paper-10356049.
The physiological relevance of the repression was supported by the facts that repression was apparent in multiple cell lines, endogenous AP-1 target genes were repressed, and similar concentrations of NIC-1 were required for CSL-dependent activation and AP-1 repression. Paper-9156281.
In addition, we have found several placental-specific hypersensitive sites downstream of the hCS-L and hCS-A genes, which might reflect the presence of enhancer elements similar to that located downstream of the hCS-B gene (Walker et al. (1990) J. Biol. Chem. 265, 12940). Paper-7652007.
CSL, licensee of UniQuest's HPV technology, and Aventis Pasteur MSD (a joint venture between Merck & Co and Aventis) are jointly developing a vaccine for the potential prophylaxis of genital warts and cervical cancer caused by human papilloma virus infection. Paper-10428144.
We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 ( ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 ( MAML-1). Paper-11324968.
Short (< or =25 residues) WFP-containing peptides encoded by the four mammalian Notch genes have similar affinities to BTD; thus, activity differences between paralogues either result from other regions of NICD and CSL or from differences in interaction with downstream components. Paper-12384855.
The human growth hormone gene ( GH-N) is located in a cluster of five highly homologous genes that are coordinately expressed in pituitary ( GH-N) and in placental tissues (the chorionic-somatomammotropin-like gene, the GH-variant gene and the two chorionic somatomammotropin genes). Paper-1126408.
The intracellular domain of Notch interacts with the DNA-binding factor, CSL, resulting in transactivation at various promoters, in particular those of various basic helix-loop-helix factors of the HES (Hairy and Enhancer of Split) and HRT families ( Hairy-Related Transcription factor). Paper-10767332.
In terms of relative densities of immunoreactive SP- and CGRP-like peptides, the CSL dorsally and the DS-impar ligament had the highest relative densities of nerve fibres followed by the navicular bone, the palmar aspect of CSL and the DDf tendon bordering the navicular bursa. Paper-253870.
Three plasma-derived concentrates were tested: Fandhi (Grifols) which contains VWF with a final ratio of approximately 1 ( VWF IU per IU FVIII:C); Haemate ( CSL Behring) with a ratio of 2.5 and Haemofil M (Baxter), a monoclonal antibody-purified concentrate containing only trace amounts of VWF. Paper-13436626.
Ligand binding induces proteolytic cleavages in NOTCH1 that release its intracellular domain (ICN1), which translocates to the nucleus and activates target genes by forming a short-lived nuclear complex with two other proteins, the DNA-binding factor CSL and a Mastermind-like ( MAML) coactivator. Paper-12077010.
Adhesion to CS5, when presented to cells as an immobilized IgG conjugate, was blocked by antifunctional monoclonal antibodies directed against either the alpha 4 or beta 1 integrin subunits, but not by antibodies against other subunits, implying that alpha 4 beta 1 is also the receptor for CS5. Paper-26620.
Nuclear complex, consisting of CSL ( RBPSUH), NICD, Mastermind ( MAML), p300 and histone acetyltransferase (HAT), then induces transcriptional activation of Notch target genes, such as HES1, HES5, HES7, HEY1, HEY2 and HEYL. Paper-11367216.
The mechanism of Notch signalling is thought to require cleavage of the receptor in response to ligand binding, movement of the receptor's intracellular domain to the nucleus, and binding of that intracellular domain to a CSL (for CBF1, Suppressor of Hairless, LAG-1) protein. Paper-8503025.
Human enterotoxigenic Escherichia coli (ETEC) producing colonization factor antigen III ( CFA/III) and coli surface antigens 4, 5, and 6 (CS4, CS5, and CS6) of CFA/IV were examined ultrastructurally and for ability to adhere to human small intestinal enterocytes and to cultured human intestinal mucosa. Paper-6411283.
The results of this validation study are discussed in relation to the results of other studies in which lots 5 and 14 of K12 and early and late batches of a suspension prepared from another sarcoid spleen at the Commonwealth Serum Laboratories designated CSL and provided by Dr T.H. Hurley in Melbourne were employed. Paper-2572967.
The regulatory modules that control expression of T-lineage genes frequently include binding sites for a core set of regulators that set the T-cell-specific background for signal-dependent control, including GATA-3, Notch/ CSL, c-myb, TCF-1, Ikaros, HEB/ E2A, Ets, and Runx factors. Paper-11346488.
CSL ( CBF1, Suppressor of Hairless, Lag-1) is a transcription factor that is responsible for activating the genes downstream of the Notch signalling pathway, a pathway that is essential for the development of the nervous system and the differentiation of the haematopoietic system among others. Paper-11087906.
Kti11p, DESR1, both belonging to a protein family characterized by a CSL zinc finger domain, and the co-catalytic zinc-protein PML containing a Zn(2+) binding domain called RING or C(3)HC(4) finger are all structurally determined by NMR although the zinc sites are silent to this spectroscopical method. Paper-12890175.
Three aECM variants were studied: aECM 1 contains lysine residues periodically spaced within the protein sequence and three repeats of the CS5 domain of fibronectin, aECM 2 contains periodically spaced lysines and three repeats of a scrambled CS5 sequence, and aECM 3 contains lysines at the protein termini and five CS5 repeats. Paper-9931934.
One hundred nine ETEC strains that were isolated from seven different laboratories in various regions of the country were tested for CFAs by using monoclonal antibodies against CFA/I and E. coli surface antigens CS1, CS2, and CS3 of CFA/II and CS4 and CS5 of CFA/IV; a polyclonal antiserum against CS6 was used. Paper-51192.
A proteolytic cascade induced by ligand stimulation results in release of the intracellular Notch domain from the cell membrane, allowing it to enter the nucleus and form a complex with a DNA-bound transcription factor called CSL ( CBF-1/ RBP-J kappa, Suppressor of Hairless, and Lag-1) and a coactivator of the Mastermind family. Paper-12696089.
Expression of a human sequence related to Drosophila Mam ( hMam-1) in mammalian cells augments induction of Hairy Enhancer of split (HES) promoters by Notch signaling. hMam-1 stabilizes and participates in the DNA binding complex of the intracellular domain of human Notch1 and a CSL protein. Paper-8983318.
Moreover, by using luciferase as a reporter gene under the control of a CSL/RBP-Jk/ CBF-1-dependent promoter in the dlk-negative, Notch1-positive Balb/c 14 cell line, we found that addition of synthetic dlk EGF-like peptides to the culture medium or forced expression of dlk decreases endogenous Notch activity. Paper-10792965.
Our findings are consistent with a model for complex assembly in which the RBP-J kappa- associated molecule domain of Notch increases the effective concentration of the ankyrin domain for its binding site on the Rel-homology region of CSL, enabling docking of the ankyrin domain and subsequent recruitment of the Mastermind-like coactivator. Paper-12696089.
The Human Growth Hormone/Chorionic Somatomammotropin ( hGH/CSH) gene cluster (48 kb) at 17q22-24, consisting of one pituitary-expressed postnatal ( GH1) and four placental genes ( GH2, CSH1, CSH2, and CSHL1) may contribute to common variation in intrauterine and infant growth, and also to the regulation of feto-maternal and adult glucose metabolism. Paper-13015441.
Double NANOG-binding sites, CSL/ RBPSUH- binding site and TATA-box in HES1 promoter, NANOG-, SOX2-, POU5F1/ OCT3/ OCT4- binding sites and TATA-box in HES3 promoter, double CSL- binding sites in HES5 promoter, SOX2-, POU-binding sites and TATA-box in HES6 promoter, and CSL- binding site in HEY1, HEY2 and HEYL promoters were evolutionarily conserved. Paper-13326457.
These synonyms are used for gene CSHL1 (chorionic somatomammotropin hormone-like 1): MGC149868, Lactogen-like, hCS-L, CSL, CSHP1, CS-5, Chorionic somatomammotropin-like, Chorionic somatomammotropin hormone-like 1.
These accession numbers are used for gene CSHL1: Q0VDB2 (UNIPROT__AC), CR595432 (NCBI_GENBANK__AC), BC029365 (NCBI_GENBANK__AC), B7Z6E9 (UNIPROT__AC).
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