Click here for the function of FGF7.
Edit this page in Wiki Genes - FGF7 or see Wiki Gene.
KGF may play a role in pathogenesis of ADPKD. Paper-11470291.
The role of WT1 and FAK in KGF signaling was investigated. Paper-14303468.
Both GH and KGF increase colonic mucosal growth in this model. Paper-11580029.
Both GH and KGF modestly increased colonic crypt depth after SBR. Paper-11580029.
ICAM-1 and VCAM-1 mRNA expression were also inhibited by KGF. Paper-9701163.
Ki67, K15 and KGF/ KGFR were significantly upregulated at early-mid SL stages. Paper-15290406.
Expression of bFGF, KGF and FGF receptors on normal oral mucosa and SCC. Paper-660613.
There was absent or weak bFGF, KGF, flg, and bek immunoreactivity in normal kidneys. Paper-8534963.
CONCLUSIONS: KGF and KGFR expression was upregulated in ADPKD kidney tissues. Paper-11470291.
Short hairpin- KGF transfection in KLM-1 cells reduced VEGF-A expression in the cells. Paper-13256320.
However KGF, unlike EGF and TGF beta, allows keratinocytes to differentiate normally. Paper-211779.
Expression of FGF7 and its receptor ( FGFR2) were determined by the RNase protection assay. Paper-9659662.
AKT and MAPK signaling in KGF-treated and UVB-exposed human epidermal cells. Paper-13301280.
The effect was selective to KGF, as EGF-induction was independent on ECM composition. Paper-1804533.
High-affinity FGFR2 binding peptides derived from the native epitope of the KGF ligand. Paper-13740952.
Interestingly, AMH inhibited the stimulatory actions of KITL, bFGF, and KGF. Paper-13357279.
The human homologue of the yeast CHL1 gene is a novel keratinocyte growth factor-regulated gene. Paper-717733.
KGF might increase the endocrine resistance via decreasing ER-alpha, PR and PTPgamma as well. Paper-12059668.
Interestingly, IL-7 administration also led to a significant increase in KGF expression. Paper-10801147.
RESULTS: KGF and KGFR mRNA expression in ADPKD cysts was higher than in normal kidney tissues. Paper-11470291.
In conclusion, KGF decreases ICAM-1 and VCAM-1 expression and neutrophil adherence in BEC. Paper-9701163.
HGF expression in OSE was found to be stimulated by KGF, HGF, and KL. Paper-8469073.
Role of keratinocyte growth factor in the pathogenesis of autosomal dominant polycystic kidney disease. Paper-11470291.
KGF induces lipogenic genes through a PI3K and JNK/ SREBP-1 pathway in H292 cells. Paper-11117591.
The protein core of the proteoglycan perlecan binds specifically to fibroblast growth factor-7. Paper-2149123.
These results suggest that the KGF/ KGFR system has a critical role in early lung organogenesis. Paper-749771.
Higher heparin concentrations inhibited KGF, but not aFGF, binding and signaling. Paper-1741945.
A weak but significant linear relationship between PSA and KGF (p = 0.034) was found in patients with BPH. Paper-8577014.
A two to three times and a 1.5 to four times over-expressions were observed for bFGF and KGF, respectively. Paper-8900832.
SP-C- KGF transgenic mice failed to survive gestation to term, dying before E17. Paper-530786.
HGF protein, KGF protein, and EGF proteins were detected in rabbit lacrimal gland tissue. Paper-1965486.
In human EC cell lines (TE-1, TE-8 and TE-11), KGFR mRNA was expressed but no KGF mRNA was detected. Paper-13422319.
TNF-alpha (30 ng/ml) increased KGF mRNA expression by 535% at 24 h, with induction first seen at 8 h. Paper-790346.
CONCLUSION: ER-alpha expression is associated with KGF-induced proliferation of breast cancer cells. Paper-14487855.
Hepatocyte growth factor or KGF rapidly activated MAPK in corneal epithelial cells. Paper-1474484.
GLP-2 significantly stimulated mRNA expression of VEGF and TGF-beta, but not of KGF in CCD-18Co. Paper-12675196.
RESULTS: Our data showed that KGF significantly suppressed ER-alpha, PR and PTPgamma expression in MCF-7 cells. Paper-12059668.
KGF was also shown to increase cellular proliferation and activated the MAPK p44/p42 cascade. Paper-10751892.
However, only inhibition of Src and ERK could block KGF- stimulated secretion of uPA and MMP-9. Paper-9269416.
METHODS: Rabbit corneas were stimulated with EGF, HGF, and KGF (10 ng ml(-1)) for different times. Paper-9668875.
KGF messenger RNA expression in OSE was found to be stimulated by KGF and HGF, but not KL. Paper-8469073.
In this report we examined the specific binding of FGF7 to various domains and subdomains of perlecan protein core. Paper-2149123.
Differentiated prostate cell survival depended on E-cadherin and PI3K, but not KGF, androgen, AR or MAPK. Paper-14198584.
The predicted FGF-10 protein is most related to keratinocyte growth factor ( KGF, or FGF-7). Paper-1453860.
In a search for KGF-regulated genes, we identified the gene encoding the nucleoside diphosphate kinase NM23-H1. Paper-12438597.
The activation of MAPK (p42 and p44) by HGF or KGF was transient and decreased gradually within 1 hour. Paper-1474484.
Papillary fibroblasts produced a higher ratio of GM-CSF to KGF than did corresponding reticular fibroblasts. Paper-10278135.
Kinase inhibitor studies showed that induction of DeltaNp63alpha expression by KGF is mediated via the p38 pathway. Paper-14600177.
The expression levels of KGF and IGF-II were decreased with proliferative aging but not by growth arrest of young cells. Paper-9554555.
The expression of iNOS, KGF and KGFR in laser wounds was more intense than in electrocautery wounds. Paper-9384271.
KGF suppressed ER-alpha, PR and PTPgamma mRNA to a maximal inhibition at 20 ng/ml by 88%, 57% and 61%, respectively. Paper-12059668.
KGF did not significantly change the effects of EGF, TGF beta or IFN gamma on keratin gene expression. Paper-211779.
In addition to the known interaction between HSPG and KGF, we found that the KGFR also bound heparin. Paper-1741945.
Furthermore, siRNA-mediated downregulation of cortactin inhibited KGF- and FGF10-induced migration. Paper-13210729.
These results indicate that cortactin is involved in keratinocyte migration promoted by both KGF and FGF10. Paper-13210729.
The levels of KITLG and KIT, but not GDF9 and BMP15 mRNA expression were stimulated by FGF7. Paper-13015479.
Transcripts for bFGF were detected in normal and malignant oral mucosa and KGF within connective tissue elements. Paper-660613.
Keratinocyte growth factor stimulates CFTR-independent fluid secretion in the fetal lung in vitro. Paper-853092.
Keratinocyte growth factor ( KGF) decreases ICAM-1 and VCAM-1 cell expression on bronchial epithelial cells. Paper-9701163.
Keratinocyte growth factor ( KGF) and vascular endothelial growth factor ( VEGF) are key regulators of lung development. Paper-12628465.
These observations suggest that KGF/ aFGF together with proteoglycans may help regulate rat but not human liver growth. Paper-8221377.
Interestingly, p63 was highly expressed in KGF-treated limbal epithelial sheets but not in those treated with HGF. Paper-14600177.
We found a significant dose-dependency between leptin incubation and production of KGF and EGF at the RNA and protein level. Paper-10771612.
Transforming Growth Factor beta ( TGF-beta), VEGF and KGF mRNA levels were determined by RT-PCR. Paper-12675196.
CONCLUSIONS: Hepatocyte growth factor and KGF activate Ras- MAPK pathways in human corneal epithelial cells. Paper-1474484.
None of the SCC cell lines was positive for KGF mRNA, whereas all cell lines were highly positive for aFGF and bFGF. Paper-1566867.
Immunolocalization of the keratinocyte growth factor in benign and neoplastic human prostate and its relation to androgen receptor. Paper-2041132.
Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells. Paper-13577509.
In accordance with the in vivo findings, KGF expression was detected only in ER alpha-positive MCF7 and ZR-75-1 cells in vitro. Paper-10640066.
Our data demonstrate that PAK4 is an important mediator of the anti-apoptotic effects of KGF on epithelial cells. Paper-9871697.
Treatment of RCE cells with TGF-beta1, -beta2, or -beta3, HGF, and KGF increased mRNA in PAF-R; however, bFGF had no effect. Paper-8501723.
Expression of KGF protein was up-regulated markedly by IL-1 beta and moderately by PDGF-BB, especially in limbal fibroblasts. Paper-1135961.
The same growth factors, and in addition KGF, all stimulated motility in keratinocytes, but TGFbeta and alphaFGF again had no effect. Paper-11278464.
RESULTS: KGF promoted differentiation of H2 cells to goblet cells as reflected by induced ITF expression. Paper-8782594.
Extracellular matrix-dependent activation of syndecan-1 expression in keratinocyte growth factor-treated keratinocytes. Paper-1804533.
Mutation of the SREBP-1 binding site in the SCD-1 promoter abolished the effect of KGF on SCD-1 transcription. Paper-11117591.
Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4. Paper-9252278.
We investigated here the role of KGF in modulating AKT and MAPK activity during differentiation of human keratinocytes. Paper-13301280.
The protective KGF- mediated SP-A production is abolished in mice given alloreactive T cells plus Cy. Paper-8607902.
Heparan sulfate proteoglycan modulates keratinocyte growth factor signaling through interaction with both ligand and receptor. Paper-1741945.
Keratinocyte growth factor- induced PCNA expression is reversible upon cessation of KGF administration. Paper-334319.
Morphologic observation showed that KGF induced proliferation but did not cause significant differentiation of 32D/ K-sam cells. Paper-9202678.
In the dysplastic kidneys, there was strong immunoreactivity of bFGF and KGF and their receptors in the epithelium of primitive tubules. Paper-8534963.
Telomerase rescues the expression levels of keratinocyte growth factor and insulin-like growth factor-II in senescent human fibroblasts. Paper-9554555.
The stimulatory effect of IL-1alpha on DPC VEGF, GM-CSF, KGF, and IL-8 expression was also evident at the mRNA level for these cytokines. Paper-12235818.
Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells. Paper-13237092.
Moreover, bFGF and KGF act in parallel in the prostate, one stimulating the prostatic stroma and the other one stimulating the epithelium. Paper-8900832.
Genotyping of SNPs in the genes found associations between NS CLP and SNPs in FGF3, FGF7, FGF10, FGF18, and FGFR1. Paper-13153989.
Furthermore, pretreatment with KGF prevented the loss of actin and MHC from the actin cytoskeleton but did not prevent the decrease in TER. Paper-10372354.
PLAC1, a trophoblast-specific gene, is expressed throughout pregnancy in the human placenta and modulated by keratinocyte growth factor. Paper-9637630.
Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin 5. Paper-1388753.
In patients with serum PSA less than 10 ng./ml. serum KGF levels were significantly higher in the BPH compared to the prostate cancer group. Paper-8577014.
Expression of EGF receptor, bFGF receptor, PDGF receptor and keratinocyte growth factor ( KGF) receptor in the TM has also been reported. Paper-9338123.
The growth-promoting effect of KGF on limbal epithelial cells is mediated by upregulation of DeltaNp63alpha through the p38 pathway. Paper-14600177.
125I- KGF binding was completed efficiently by acidic FGF ( aFGF) but with 20-fold lower efficiency by basic FGF ( bFGF). Paper-6836516.
RESULTS: Stimulation with EGF, HGF, or KGF for 12 hr induced 12-LOX mRNA expression in rabbit corneal epithelial cells. Paper-9668875.
The KGF- stimulated tumor cell growth was almost completely inhibited by heparin or epidermal growth factor ( EGF). Paper-1267583.
RESULTS: Most organ cultures detected the presence of measurable levels of HGF, with a relative paucity of KGF and TGF-beta activity. Paper-9338434.
IL-8, VEGF, KGF, and TIMP-1 levels were determined ( enzyme-linked immunosorbent assay) in wound fluid after each 4 weeks of treatment. Paper-10044624.
Parathyroid hormone-related protein is a positive regulator of keratinocyte growth factor expression by normal dermal fibroblasts. Paper-1962079.
In addition, GLP-2 (but not GH or KGF) prevented the SBR-induced oxidation of the glutathione/ GSSG pools in both ileum and colon. Paper-11580029.
Moreover, short hairpin- KGFR transfection in MIA PaCa-2 cells reduced the stimulatory effect of exogenous KGF on VEGF-A expression. Paper-13256320.
Human androgen receptor was uniformly expressed in the same secretory glandular cells that were positive for KGF in BPH and prostate cancer. Paper-2041132.
Total RNA were isolated and real-time PCR was employed to identify ER-alpha, PR and PTPgamma gene expressions in response to KGF and KGF-13. Paper-12059668.
The standard medium with EGF was used as the positive control and the standard medium without EGF or KGF-2 was used as the negative controls. Paper-9342100.
In contrast, the glioblastoma cell line, A-172, that expressed the bFGF receptor showed a mitogenic response to bFGF but not to KGF. Paper-7921717.
The level of keratinocyte growth factor ( KGF) mRNA was also examined as a possible mechanism for upregulation of EGFr ligands. Paper-1477333.
Keratinocyte growth factor regulates proliferation and differentiation of hematopoietic cells expressing the receptor gene K-sam. Paper-9202678.
These results suggest that KGF may play an important role in the regulation of VEGF gene expression and angiogenesis during wound healing. Paper-16009363.
Multiple protein kinase C ( PKC) isoforms have been associated with the epidermal keratinocyte ( KC) granular layer differentiation program. Paper-13988298.
Western blot analysis and immunohistochemical staining confirmed the down-regulation of ER-alpha, PR and PTPgamma by KGF in protein levels. Paper-12059668.
KGF- induced fluid accumulation is driven by CFTR-independent Cl- transport and associated with decreased expression of alpha-ENaC. Paper-853092.
The addition of HGF and KGF resulted in increased DNA synthesis in each cell type, but no effect of added TNF-alpha was found. Paper-8437747.
Thus, perlecan protein core should be considered a novel biological ligand for FGF7, an interaction that could influence cancer growth and tissue remodeling. Paper-2149123.
Cellular response to KGF was verified through proliferation assays and Western blot analysis for mitogen-activated protein kinase ( MAPK). Paper-10751892.
We found that KGF induces a dose- and time-dependent increase in Akt kinase activity and that, as expected, activation of Akt via KGF is PI3K dependent. Paper-10806277.
Because HGF lacks true epithelial specificity, it may have less potential than KGF and FGF-10 as a targeted therapy to facilitate lung epithelial repair. Paper-9427665.
Growth hormone, KGF, EGF, teduglutide, GM-CSF/ G-CSF have entered early clinical trials, whereas others are currently in preclinical evaluation. Paper-15581062.
KGF and KGFR protein expression was also higher in ADPKD cysts and was localized to cyst-lining epithelial cells, tubular and interstitial cells. Paper-11470291.
Identification of keratinocyte growth factor as a target of microRNA-155 in lung fibroblasts: implication in epithelial-mesenchymal interactions. Paper-13945748.
Furthermore, KGF enhanced the DNA synthesis of the esophageal cancer cells, TE-1, in a dose-dependent manner, while the effect of bFGF was not substantial. Paper-7921717.
Mouse Fgf7 ( fibroblast growth factor 7) and Fgf8 ( fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively. Paper-234412.
IL-1 alpha and IL-1 beta at 10 ng/ml upregulated the levels of HGF and KGF mRNAs and proteins in cultured human corneal fibroblasts. Paper-1115728.
CONCLUSION: EGF, HGF and KGF had differential effects on cell cycle- and differentiation-related gene expression in corneal epithelial cells. Paper-8898807.
By contrast, the major basal type I keratin, K14, was upregulated by TGF-beta1, whereas TNF-alpha, EGF, and KGF had no effect. Paper-2102574.
Conversely, adenovirus-mediated overexpression of a dominant negative form of SREBP-1 inhibited the KGF effect on FAS and SCD-1 expression. Paper-11117591.
Control AECs grown in the absence of KGF through Day 8 express increasing levels of AQP5, consistent with transition toward the AT1 cell phenotype. Paper-1388753.
In addition, UVB exposure increases KGF expression, and KGF treatment induces tyrosinase ( TYR) expression in primary melanocytes. Paper-14163489.
METHODS: Primary cultures human corneal epithelial cell (HCE) were treated with 25 ng/ml of EGF, 25 ng/ml HGF, 25 ng/ml KGF, or vehicle for 8 hours. Paper-8898807.
Indirect immunohistochemistry was performed using the Strept-ABC method with four antibodies: bFGF, KGF, FGF receptor (flg), and KGF receptor (bek). Paper-8534963.
PLAC1 expression increases during trophoblast differentiation: evidence for regulatory interactions with the fibroblast growth factor-7 ( FGF-7) axis. Paper-10786504.
Fibroblast growth factor receptor 4 is a high affinity receptor for both acidic and basic fibroblast growth factor but not for keratinocyte growth factor. Paper-7584443.
Keratinocyte growth factor protects murine hepatocytes from tumor necrosis factor-induced apoptosis in vivo and in vitro. Paper-1458582.
KGF induces keratinocyte motility and cytoskeletal rearrangement, whereas a direct role of FGF10 on keratinocyte migration is not clearly established. Paper-13210729.
In this work, the expression of two FGFs: bFGF (or FGF2) and KGF (or FGF7), was studied in RT-PCR and semi-quantified in densitometry. Paper-8900832.
Estrogen receptor- associated expression of keratinocyte growth factor and its possible role in the inhibition of apoptosis in human breast cancer. Paper-10640066.
GLP-2 treatment increased jejunal villus height and jejunal total mucosal height compared with effects of resection alone or resection with GH or KGF treatment. Paper-11580029.
FGF-7 and its receptor, FGFR-2b, exhibited spatial overlap with PLAC1 suggesting these regulatory interactions are physiologically relevant during gestation. Paper-10786504.
In support of this hypothesis, treatment of keratinocytes for 5 min with either KGF CM or purified TGF alpha resulted in EGFR autophosphorylation. Paper-190436.
Androgen receptor activation in prostatic tumor cell lines by insulin-like growth factor-I, keratinocyte growth factor, and epidermal growth factor. Paper-134056.
The increase in proliferation could not be observed by simultaneous incubation with salivary leptin and specific antibodies against either leptin or KGF (P<0.001). Paper-10771612.
Keratinocyte growth factor/ fibroblast growth factor-7- regulated cell migration and invasion through activation of NF-kappaB transcription factors. Paper-13125236.
AR activation by IGF-I, KGF, and EGF was completely inhibited by the pure AR antagonist casodex, showing that these effects are AR mediated. Paper-134056.
CONCLUSION: EGF, TGF-alpha and bFGF, but not KGF, have mitogenic activity on hamster sebocytes, and all these growth factors act as antilipogenic factors. Paper-9425187.
Treatment with KGF tended to decrease VCAM-1 expression in TNF- and TNF + IL-4-stimulated BEAS-2B ( P = n.s. and P < 0.05, 14 and 15% inhibition, respectively). Paper-9701163.
Conversely, adenovirus-mediated overexpression of a constitutively active form of SREBP-1c mimicked the effect of KGF on lipogenic enzymes and lipogenesis. Paper-12066521.
Trophoblast-specific expression throughout gestation and responsiveness to KGF are consistent with a fundamental role for PLAC1 at the maternal-fetal interface. Paper-9637630.
In contrast, the same KGF treatment in irradiated mice given T cells and Cy failed to up-regulate SP-A mRNA and protein expression. Paper-8607902.
In fibroblasts syndecan-1 is induced by FGF-2 and in keratinocytes by epidermal growth factor ( EGF) and keratinocyte growth factor ( KGF). Paper-2016783.
Hepatocyte growth factor was more detrimental than KGF, resulting in an aberrant epithelium and mass differentiation of keratocytes into myofibroblasts. Paper-10788527.
Specifically, Hic-5/ ARA55 expression influences androgen- induced keratinocyte growth factor ( KGF) expression in WPMY-1 prostate stromal cells. Paper-13124868.
Total protein amount, bFGF, HGF, KGF and TGF-β(1) showed lower levels in samples from donors with higher gestational ages and donor ages, for all groups. Paper-15444897.
Increased local expression of bFGF and KGF and their receptors in primitive tubules suggests that bFGF and KGF may play an important role in the development of RD. Paper-8534963.
The aim of this study was to investigate the immunoactivity of bFGF and KGF and their receptors in the dysplastic kidney in order to further understand the pathogenesis of RD. Paper-8534963.
Cortactin involvement in the keratinocyte growth factor and fibroblast growth factor 10 promotion of migration and cortical actin assembly in human keratinocytes. Paper-13210729.
Their selective ligand binding properties were confirmed by the specific neutralization of acidic FGF or KGF mitogenic activity using D2 or D3 HFc, respectively. Paper-7926574.
PAR-1 mediated FGF-7 production by cultured stromal cells was assessed by RT-PCR and immunoassays, and verified by small interfering RNA (siRNA). Paper-14328211.
KGF induced increased numbers of TECs and intrathymic interleukin-7 ( IL-7) production and reorganization of cortical and medullary architecture. Paper-13141973.
Exogenous KGF increased VEGF-A expression and release in MIA PaCa-2 cells, and PANC-1 cells stably transfected to overexpress KGF-exhibited increased VEGF-A expression. Paper-13256320.
We show that a dominant negative mutant of PAK4 blocks KGF-mediated inhibition of caspase-3 activation in epithelial cells subjected to oxidant stress. Paper-9871697.
Hepatocyte growth factor and keratinocyte growth factor enhance IL-1- induced IL-8 secretion through different mechanisms in Caco-2 epithelial cells. Paper-15692115.
Basic FGF binds with high affinity to FGFR1, FGFR2, and FGFR4, whereas KGF does not interact with these receptors and can only bind an isoform of FGFR2 known as the KGFR. Paper-443276.
Northern analysis indicated that mRNA for FGF receptor 2b/ KGF receptor was drastically down-regulated within 1 wk in aFGF-induced mesenchymal NBT-II cells. Paper-8052720.
Furthermore, the CHL1 gene product and the protein encoded by the novel KGF-regulated gene were identical in size, indicating that we had cloned the human CHL1 homologue. Paper-717733.
AECs treated with KGF from Day 4 or 6 exhibit a decrease in AQP5 expression through subsequent days in culture, as well as an increase in expression of surfactant apoproteins. Paper-1388753.
The removal of keratinocyte ( KC) nuclear DNA by deoxyribonucleases (DNases) is an important step in the formation of normal stratum corneum ( SC). Paper-12376969.
In the endometrial tissue, selective expression of KGF in stromal cells and KGFR in epithelial cells supports the paracrine function of KGF in epithelial tissue. Paper-7631465.
Coimmunoprecipitation approaches indicated that Hrs is recruited to KGFR only after KGF treatment, although it is not tyrosine phosphorylated by the ligand. Paper-13838023.
We found that KGF stimulated transepithelial Cl(-) transport, but the number of cystic fibrosis ( CF) transmembrane conductance regulator ( CFTR) transcripts fell. Paper-8882346.
KGFR mRNA was expressed in eight pancreatic cancer cell lines, whereas the KGF mRNA was detected in seven of the cell lines and was absent in MIA PaCa-2 cells. Paper-13256320.
IL-6 (200 units/ml), TGF-beta 1 (5 ng/ml) and IFN-gamma (200 units/ml) did not change the level of KGF mRNA at 24 h in human fibroblasts under the same conditions. Paper-790346.
Cyclophosphamide prevents systemic keratinocyte growth factor- induced up-regulation of surfactant protein A after allogeneic transplant in mice. Paper-8607902.
In this study, we analyzed the mechanism of KGF modulation of goblet cell differentiation through characterization of its effects on ITF gene expression. Paper-8782594.
In summary, we conclude that KGF requires both PI3K and JNK signaling pathways to induce SREBP-1, which in turn induces SCD-1 and FAS expression in H292 cells. Paper-11117591.
In cell-proliferation studies, epithelial cells treated with EGF, HGF, or KGF for 24, 48, and 72 hr were measured with a CyQUANT cell-proliferation assay kit. Paper-9668875.
Collectively, our results indicate that ER alpha may be involved in KGF expression, and that KGF may play antiapoptotic roles, rather than mitogenic, in human breast cancer. Paper-10640066.
Human keratinocytes were cultured with KGF in the presence or absence of a KGF receptor (KGFR) inhibitor or mitogen-activated protein kinase ( MAPK) inhibitors. Paper-14657450.
In the present study, ER-alpha, two estrogen-regulated genes, PR and PTPgamma, KGF and their relationship with endocrine resistance in human breast cancer cells were investigated. Paper-12059668.
There was no association between KGF and tumor grade or stage but there was a strong positive linear relationship between PSA and KGF (p = 0.006, R(2) = 68.3%) in low grade tumors. Paper-8577014.
The latter is unique among FGFs in that it binds and signals only through the FGF receptor (FGFR2b) isoform KGF receptor ( KGFR) expressed specifically by epithelial cells. Paper-1453860.
Our results show that the mechanisms of action of KGF are dose-dependent and that a sustained activation of the MAPK signaling cascade causes a negative regulation of AKT. Paper-13301280.
Using FAS and SCD-1-luciferase promoter constructs, we observed that KGF stimulated the transcription of these promoters and that exogenous cholesterol inhibited the induction. Paper-11117591.
Expression of these FGF varied throughout hair growth cycle: mRNA expression of FGF18 and 13 peaked at telogen; FGF7 and 10 at anagen V; and FGF5 and 22 at anagen VI. Paper-11216785.
CONCLUSION: These findings suggest that EGF, HGF, and KGF stimulate 12(S)-HETE production in rabbit corneal epithelial cells through gene induction of 12-LOX. Paper-9668875.
These mesenchymal-epithelial cell interactions are in part mediated by keratinocyte growth factor ( KGF), hepatocyte growth factor ( HGF), and Kit ligand (KL). Paper-1398950.
KGF elicits its activity through binding to and activation of KGF receptor, a splicing transcript variant of fibroblast growth factor receptor 2 ( FGFR2). Paper-1718942.
These therapies include treatment with keratinocyte growth factor ( KGF), growth hormone ( GH), LHRH agonists, interleukin 7 ( IL-7) and interleukin 15 ( IL-15). Paper-12658976.
Furthermore, after approximately 24 h, KGF as well as TGF alpha induced EGFR down-regulation based on Western blot analysis and 125I-EGF binding. Paper-190436.
Thecal cell-derived KGF and HGF can stimulate granulosa cell-derived KL expression, and KL, in turn, can stimulate thecal cell-derived KGF and HGF expression. Paper-1398950.
Now, we demonstrate that the activation of this enhancer by keratinocyte growth factor ( KGF) is modulated by the components of the extracellular matrix ( ECM). Paper-1804533.
The mitogenic activity of FGF-10 could be distinguished from that of KGF by its different sensitivity to heparin and lack of neutralization by a KGF monoclonal antibody. Paper-1453860.
Northern blot analysis showed higher expression of surfactant protein C ( SP-C) mRNA (a marker for alveolar epithelial type II cells) in KGF-treated fetal lungs. Paper-694923.
Overexpression of a dominant-negative form of SREBP-1 decreased lipogenesis and decreased the induction of fatty acid synthase and stearyl coenzyme A desaturase-1 by KGF. Paper-12066521.
Keratinocyte growth factor ( KGF) interacts with its specific receptor ( KGFR), and elicits the proliferation and/or differentiation of the various types of epithelial cells. Paper-13764520.
Effects of HGF, KGF, and kinase inhibitors on mitogen-activated protein kinase ( MAPK) activation were evaluated by western blot analysis and enzymatic assays. Paper-1474484.
RESULTS: Our results showed that in MCF-7 cells KGF increased Akt phosphorylation and induced ER-alpha mRNA expression which could be blocked by a PI3K/Akt pathway inhibitor, LY. Paper-13918227.
Exposure of AECs to KGF from Day 0 results in decreased AQP5 expression, retention of a cuboidal morphology, and greater numbers of lamellar bodies relative to control on Day 8 in culture. Paper-1388753.
Secretion by pulmonary fibroblasts of hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF) is crucial to an effective epithelial repair after lung injury. Paper-10767149.
MATERIALS AND METHODS: A cell culture wounding model was used to study the effects of WT1 and FAK down-regulation on KGF-induced proliferation and motility in breast cancer cells. Paper-14303468.
The KGFR is encoded by the bek/FGFR-2 gene, whose alternative transcript specifies a receptor with high affinity for aFGF and bFGF, but no detectable binding of KGF. Paper-89520.
By double labelling we found that these GLP-2 receptor immunoreactive cells also produce smooth muscle actin and keratinocyte growth factor ( KGF). Paper-10792495.
Since KGF has recently emerged as an autocrine mediator in prostate cancer, we investigated the role KGF plays in the human prostate and its relationship to androgen receptor ( AR). Paper-2041132.
These results suggest that the growth factors HGF and KGF may play a role in enhancing IL-1- stimulated production of IL-8 by epithelial cells during mucosal inflammations. Paper-15692115.
These findings suggest that administration of rhKGF and over-expression of endogenous KGF genes in colorectal cancer cells increase VEGF-A production and may relate to angiogenesis in cancer. Paper-13577509.
The mutations in the FGF genes were not known to play role in human disease until the year 2000, when mutations in FGF23 were found to cause hypophosphatemic rickets. Paper-13964999.
The fibroblast growth factor binding protein is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: implications for epithelial repair. Paper-11033255.
The enhancing effect of KGF was not affected by wortmannin, but was suppressed by triciribine, suggesting that the effect of KGF was through a PI3K-independent activation of AKT. Paper-15692115.
In the present study we observed that recombinant human KGF enhanced several parameters of cellular motility in ER-positive cells but not in ER-negative cell lines. Paper-10574169.
Immunofluorescence and immunoelectron microscopy analysis showed that KGFR internalization triggered by either KGF or FGF10 occurs through clathrin-coated pits. Paper-12601885.
The possibility that KGF influences the induction of keratin gene expression by other keratinocyte modulators, such as EGF, TGF beta and gamma interferon (IFN gamma), was also explored. Paper-211779.
Tissue repair was assessed by proliferating cell nuclear antigen ( PCNA) immunoreactivity and by expression of keratinocyte growth factor ( KGF) mRNA by real-time PCR. Paper-12468678.
To define the role of SREBP-1c on the induction of lipogenic genes and lipogenesis by KGF in primary cultures of rat type II cells, we used adenoviral vectors to alter levels of SREBP-1c. Paper-12066521.
Keratinocyte growth factor ( KGF), a ligand of another member of the FGF receptor family, enhanced expression of FGFR3 IIIb, but acidic FGF, the ligand for FGFR3 IIIb itself, had no effect. Paper-1013559.
Dexamethasone (10(-7) M), known to inhibit inflammatory reactions and retard wound healing, also inhibited the induction of KGF mRNA expression by IL-1 alpha, IL-1 beta and TNF-alpha. Paper-790346.
Scatchard analysis revealed that the chimera containing immunoglobulin-like domains 2 ( D2) and 3 ( D3) bound KGF and acidic FGF at high affinities comparable to the native receptor. Paper-7926574.
To investigate the effect of smokeless tobacco (ST) on (1) HGF, KGF and GM-CSF expression by buccal fibroblasts and (2) on keratinocyte and fibroblast proliferation. Paper-10734676.
Levels of Ang2, ICAM-1, and KGF, secreted by the total decidual fraction, decreased with increasing gestational age. uNK levels of Ang2 and VEGF-C also decreased with increasing gestational age. Paper-12200252.
The best characterized stromal to epithelial interactions in the cornea are mediated by the classical paracrine mediators hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF). Paper-1836364.
Expression of phosphorylation-defective IkappaBalpha (IkappaBalphaS32A,S36A), which blocked NF-kappaB activation, inhibited KGF/ FGF-7-induced gene expression and cell migration and invasion. Paper-13125236.
Intratracheal KGF is known to stimulate the expression of surfactant protein A ( SP-A), an oxidant-sensitive T cell immunomodulator produced by alveolar type II cells. Paper-8607902.
Expression of KGF transcript was down-regulated by EGF, TGF-alpha, and PDGF-BB, was markedly up-regulated by IL-1 beta, and was more pronounced in limbal than in corneal fibroblasts. Paper-1135961.
In addition, exposure of cultured human keratinocytes to IL-la or KGF resulted in accelerated expression of both cdk2 and cdk4, and this was especially striking in the case of addition of KGF. Paper-1913781.
Exposure to IL-1 alpha (20 units/ml) or IL-1 beta (100 units/ml) for 24 h increased KGF mRNA expression by 352% and 504%, respectively, with early induction seen at 2 h and maximal induction seen at 8 h. Paper-790346.
The present study examined the hypothesis that ER-alpha is involved in the KGF proliferation in MCF-7 cancer cells using small interfering RNA (siRNA) to selectively inhibit ER-alpha expression. Paper-14487855.
Induction of SREBP-1, SCD-1, and FAS by KGF was inhibited by the JNK inhibitor SP600125 and the phosphatidylinositol 3-kinase ( PI3K) inhibitor LY294002 but not by the ERK inhibitor PD98059. Paper-11117591.
Alternative cDNA sequences that are predicted to code for a soluble KGF receptor and a membrane bound, truncated HGF/ SF receptor were detected in breast epithelial cells and breast tissues. Paper-8209518.
We have examined the distribution of fibroblast growth factor receptor (FGFR)-2-IIIb, which is a high-affinity receptor for KGF and find that it is present on malignant and non-malignant epithelial cells. Paper-1036257.
RESULTS: Src, ERK, and PLD were activated in response to KGF treatment, and inhibition of these enzymes - by their specific inhibitors - decreased KGF-induced invasion in a dose-dependent manner. Paper-9269416.
Keratinocyte growth factor ( KGF), alone and in synergism with progesterone ( P) and prolactin ( PRL), is mitogenic for normal mammary epithelium (ME) in vitro. Paper-8360640.
Fibroblast growth factor-7 ( FGF-7), also known as keratinocyte growth factor ( KGF), stimulated PLAC1 mRNA expression approximately two-fold in the BeWo(b30) trophoblast cell line. Paper-10786504.
CONCLUSIONS: KGF could efficiently induce proliferation of hematopoietic cells expressing the K-sam gene without obvious induction of differentiation or exhaustion of immature progenitor cells. Paper-9202678.
Most importantly, we demonstrate that FGF-BP interacts with FGF-7, FGF-10, and with the recently identified FGF-22, and enhances the activity of low concentrations of ligand. Paper-11033255.
In this report, a second growth factor, keratinocyte growth factor ( KGF), was also found to significantly enhance IL-1-induced IL-8 secretion by Caco-2 cells, yet KGF, by itself, also had no effect. Paper-15692115.
Yield of the GST- FGF7 fusion product was improved to about 17 mg per liter per OD(600) in strain BL21(DE3)pLysS by inclusion of 10-100mM magnesium chloride (MgCl(2)) in the culture medium. Paper-10552343.
To understand the mechanism by which KGF exerts protective functions on epithelial cells, we used the yeast two-hybrid assay to identify proteins that interact with the KGF receptor ( KGFR). Paper-9871697.
We hypothesized that systemic KGF up-regulates SP-A after allogeneic BMT, and the addition of Cy may interfere with the ability of KGF to enhance SP-A production. Paper-8607902.
Proliferating cell nuclear antigen ( PCNA) expression in rat bladder is dramatically increased along the basal layer of urothelium 1, 3, 7 and 14 days after daily injections of KGF. Paper-334319.
IL-1 upregulates keratinocyte growth factor and hepatocyte growth factor mRNA and protein production by cultured stromal fibroblast cells: interleukin-1 beta expression in the cornea. Paper-1115728.
p21-activated protein kinase 4 ( PAK4) interacts with the keratinocyte growth factor receptor and participates in keratinocyte growth factor-mediated inhibition of oxidant-induced cell death. Paper-9871697.
In contrast, a significant increase in FGF-10 expression was observed in the obstructed FGF-7-null group, indicating that the compensatory pathway that functions in this model results in urothelial repair. Paper-12513794.
The protein synthesis inhibitor cycloheximide abrogated the effects of IL-1 alpha, IL-1 beta and TNF-alpha on KGF gene induction, indicating that new protein synthesis is required in the process. Paper-790346.
Interruption of p38 and ERK1/2 signaling pathways by pretreatment with inhibitors SB203580 and PD98059 and subsequent stimulation with HGF or KGF abolished the activation and nuclear localization. Paper-9661257.
In vitro, hepatocytes pretreated with KGF exhibited reduced TNF- and ActD-induced cell damage and DNA fragmentation, similar to hepatocytes pretreated with HGF and EGF. Paper-1458582.
KGF alone greatly stimulated proliferation and increased cyclin-dependent kinase ( cdk) 2 kinase activity and Retinoblastoma susceptibility gene product ( Rb) phosphorylation. Paper-10558063.
Moreover, KGF expression was closely associated with the expression of ER alpha, and the coexpression of KGF and KGFR significantly correlated with lower TUNEL index, but not with proliferative activity. Paper-10640066.
These are apparently regulated in a hierarchical fashion, since the addition of IL-1 receptor antagonist prevented induction of COX-2, IL-1 and IL-6, but not that of MMP-1 or keratinocyte growth factor ( KGF). Paper-10306509.
This cytokine also increased DPC supernatant keratinocyte growth factor ( KGF), vascular endothelial growth factor ( VEGF), IL-8 and granulocyte-macrophage colony-stimulating factor ( GM-CSF) concentrations. Paper-12235818.
Src/ ERK but not phospholipase D is involved in keratinocyte growth factor- stimulated secretion of matrix metalloprotease-9 and urokinase-type plasminogen activator in SNU-16 human stomach cancer cell. Paper-9269416.
Although moderately, KGF significantly decreased membrane ICAM-1 expression in unstimulated BEAS-2B cells (24% inhibition at 100 ng/ml) or in TNF- or TNF + IL-4-stimulated cells (22.5 and 18.7% inhibition, respectively). Paper-9701163.
Migration assays and immunofluorescence of actin cytoskeleton revealed that FGF10 is less efficient than KGF in promoting migration and exerts a delayed effect in inducing lamellipodia and ruffles formation. Paper-13210729.
In situ hybridization of the KGF-treated lungs revealed that the SP-C mRNA-expressing cells were arranged distally in the form of linear arrays, a pattern distinctly different from that in control lungs. Paper-694923.
These results suggest that lipopolysaccharide may induce proliferation of periodontal epithelial cells by upregulating keratinocyte growth factor 1 expression via the CD14 and Toll-like receptor signaling pathway. Paper-9252278.
CONCLUSION: KGF- mediated signaling employs WT1 and FAK to regulate breast cancer cell proliferation and motility and may represent therapeutic targets for the prevention of breast cancer progression. Paper-14303468.
Aberrant expression of retinol-binding protein, osteopontin and fibroblast growth factor 7 in the porcine uterine endometrium of pregnant recipients carrying embryos produced by somatic cell nuclear transfer. Paper-13077725.
The mechanisms underlying GLP-2 induced intestinal wound repair seem to involve the secretion of VEGF and, subsequently, TGF-beta from subepithelial fibroblasts, whereas KGF appeared to be less important. Paper-12675196.
RCE cells were stimulated with transforming growth factor (TGF)-beta1, -beta2, and, -beta3, basic fibroblast growth factor ( bFGF), keratinocyte growth factor ( KGF); and hepatocyte growth factor ( HGF). Paper-8501723.
This improved by about five times the yields of fully active 54ser- FGF7 after proteolytic excision of the GST portion from GST- FGF7 immobilized on heparin-Sepharose. Paper-10552343.
Although its transcript levels were similarly down-regulated in limbal fibroblasts, KGF protein levels were paradoxically up-regulated by TGF-beta 1 when added alone or with TGF-alpha or IL-1 beta. Paper-1135961.
Hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF) are key factors involved in alveolar epithelial repair, present in the bronchoalveolar lavage fluid (BALF) from patients with ALI/ARDS. Paper-12736322.
The present study was undertaken to investigate the effects of epidermal growth factor ( EGF), hepatocyte growth factor ( HGF), and keratinocyte growth factor ( KGF) on 12-LOX expression and activity. Paper-9668875.
Crosslinked hyaluronan (HA) hydrogels preloaded with two cytokine growth factors, vascular endothelial growth factor ( VEGF) and keratinocyte growth factor ( KGF), were employed to elicit new microvessel growth in vivo. Paper-11389746.
We have recently discovered that fibroblast growth factor-7 ( FGF7) binds to perlecan protein core and that exogenous perlecan efficiently reconstitutes FGF7 mitogenic activity in perlecan-deficient cells. Paper-2149123.
Since KGF and aFGF (but not bFGF) are ligands for the KGF receptor (KGFR), we explored the possibility that the TGF alpha/ EGFR pathway is an intermediary in signaling through the KGFR. Paper-190436.
We conclude that the systemic pre-BMT injection of KGF in recipients of allogeneic T cells up-regulates SP-A, which may contribute to the early antiinflammatory effects of KGF. Paper-8607902.
The subcutaneous administration of recombinant human KGF (5 mg/kg on Days -6, -5, and -4 pre-BMT) increased SP-A protein and mRNA in allogeneic T cell-recipient irradiated mice measured on Day 7 post-BMT. Paper-8607902.
Treatment of amnion epithelial cells with KGF caused an increase in the rate of [3H]thymidine incorporation, but the rate of cell replication induced by KGF was less than that induced by treatment with EGF. Paper-1206993.
Both TGF-beta1 and -beta2 dose-dependently inhibited corneal epithelial cell proliferation promoted by KGF (40 ng ml-1) and HGF (40 ng ml-1), and weakly inhibited cell proliferation promoted by EGF (4 ng ml-1). Paper-1330166.
In vivo, EBBP was found at high levels in the KGF- and epidermal growth factor-responsive basal keratinocytes of human skin, but the expression was down-regulated in the hyperthickened epithelium of skin wounds. Paper-9163675.
This is of interest because our recent studies have shown that EGF and HGF induce the epithelial cells to invade the underlying stroma while normal architecture is maintained in the presence of IGF-1 and KGF. Paper-698135.
Therefore, the increase in the number of dividing cells and in the expression level of iNOS, KGF and KGFR may induce earlier and thicker reepithelization in laser wounds than in electrocautery and scalpel wounds. Paper-9384271.
These results suggest that IL-1 alpha, IL-1 beta and TNF-alpha up-regulate KGF gene expression in fibroblasts and might be responsible for its induction following skin wounding or other injury. Paper-790346.
Our results provide evidence that IGF-I, KGF, and EGF directly activate the AR in the absence of androgens, which means that the androgen-signaling chain may be activated by growth factors in an androgen-depleted environment. Paper-134056.
In serum-free, collagen gel cell culture, dose/response (2-20 ng/ml) and time course studies showed that KGF stimulated the proliferation of PDT (not OIT) cells but synergism with P or PRL was not observed. Paper-8360640.
The modulation by KGF of membrane and mRNA expression of ICAM-1 and VCAM-1 was studied on confluent cells stimulated or not with tumour necrosis factor-alpha (TNF) (200 UI/ml) or TNF and interleukin (IL)-4 (50 UI/ml and 10 ng/ml). Paper-9701163.
Here, we show a high expression of NM23-H1 and NM23-H2 in the KGF-responsive keratinocytes of the hyperproliferative epidermis of mouse skin wounds and of patients suffering from the skin disease psoriasis. Paper-12438597.
This investigation supports our original hypothesis that KGF protects the lung epithelium by inhibiting apoptosis and that protection occurs through activation of PI3K/Akt-mediated cell survival pathway. Paper-10806277.
Keratinocyte growth factor receptor ligands induce transforming growth factor alpha expression and activate the epidermal growth factor receptor signaling pathway in cultured epidermal keratinocytes. Paper-190436.
Incorporation of 5-bromodeoxyuridine ( BrdU) at 7 and 14 days in the urothelium of KGF-treated rats parallels PCNA immunoreactivity and confirms that KGF increases DNA synthesis in urothelial cells. Paper-334319.
However, activation of FRS2 that results from KGF- stimulated FGFR2-C1 signaling is transient and is associated with a mobility shift of FRS2 not observed when this signaling molecule is activated by the C3 isoform of FGFR2. Paper-12389651.
Additionally we have examined mRNAs for Kgf receptor (KgfR), transforming growth factor alpha ( Tgf alpha), epidermal growth factor receptor ( EgfR) and cytokeratin 19 ( CK19). Paper-1060768.
RESULTS: WT1 down-regulation inhibited KGF-mediated proliferation and motility of breast cancer cells, while FAK down-regulation inhibited proliferation, but had no significant effect on cell motility. Paper-14303468.
In comparison, pancreatic carcinoma cell lines commonly demonstrated overexpression of EGFR, erbB2, TGF-alpha, Met/ HGFR, VEGF, and KGF, but they consistently showed marked down-regulation of amphiregulin mRNA expression. Paper-1485773.
However, there are no data on KGF expression in human autosomal dominant polycystic kidney disease ( ADPKD) tissue, and it is unknown whether it affects ADPKD cyst-lining epithelial cell epithelial cell proliferation. Paper-11470291.
CONCLUSION: Src, ERK, and PLD are suggested as mediators of KGF- induced invasion in SNU-16. uPA and MMP-9 are considered as downstream targets of Src and ERK whereas PLD is thought to utilize different pathways. Paper-9269416.
Transient transfection assays revealed that KGF regulates mouse ITF transcription through the goblet cell silencer inhibitor (GCSI) element, which is essential for goblet cell-specific expression of ITF. Paper-8782594.
PAR-1 activation strikingly induced FGF-7 production from cultured stromal cells mediated predominantly via ERK1/2 signaling pathway, and PAR-1 siRNA decreased the elicited FGF-7 upregulation. Paper-14328211.
Expression of K15 was also suppressed by tumor necrosis factor alpha ( TNF-alpha) and to a lesser extent by epidermal growth factor ( EGF) and keratinocyte growth factor ( KGF). Paper-2102574.
Changes in the expression of HGF and KGF mRNAs and proteins in response to stimulation of cultured corneal stromal fibroblasts with IL-1 alpha and IL-1 beta were monitored by Northern and Western blotting, respectively. Paper-1115728.
We measured the levels of inducible nitric oxide synthetase ( iNOS), endothelin, endothelin receptor, vascular endothelial growth factor ( VEGF), and keratinocyte growth factor ( KGF) gene mRNAs using reverse transcriptase PCR. Paper-9456123.
Using BeWo choriocarcinoma cells as a trophoblast model, keratinocyte growth factor ( KGF) stimulated steady-state PLAC1 mRNA expression approximately twofold by Northern analysis and quantitative real-time PCR. Paper-9637630.
METHODS: The expression and distribution of KGF and KGF receptor ( KGFR) mRNA in ADPKD cystic and normal kidney tissues were examined using quantitative real-time polymerase chain reaction ( PCR) and in situ hybridization. Paper-11470291.
Keratinocyte growth factor ( KGF/ FGF7) and fibroblast growth factor 10 ( FGF10/KGF2) regulate keratinocyte proliferation and differentiation by binding to the tyrosine kinase KGF receptor (KGFR). Paper-13210729.
METHODS: The expression of hepatocyte growth factor ( HGF), keratinocyte growth factor ( KGF), transforming growth factor-beta ( TGF-beta), and neutrophil elastase ( NE) was examined in colonic mucosal tissues. Paper-9338434.
Interestingly, epidermal growth factor ( EGF) treatment of trophoblasts had no effect on PLAC1 expression alone, but potentiated the effect of FGF-7, suggesting the presence of a regulatory interaction of the two growth factors. Paper-10786504.
One is the receptor- Grb2/Sos complex to the Ras pathway, and the other is through protein kinase C. Hepatocyte growth factor and KGF did not activate the Jak-STAT cascade components STAT1, STAT3, or Jak1 in corneal epithelial cells. Paper-1474484.
Here we have tested the activity of KGF, acidic fibroblast growth factor ( aFGF), and basic fibroblast growth factor ( bFGF) on normal adult rat and human hepatocytes and their modulation by heparin. Paper-8221377.
The biphasic effect of heparin on KGF, but not aFGF, binding and signaling suggests that occupancy of the HSPG binding site on the KGFR may specifically inhibit KGF signaling. Paper-1741945.
BACKGROUND: Keratinocyte growth factor ( KGF) produces a rapid increase in the proliferation and motility of estrogen receptor (ER)-positive breast cancer cells which is abolished by estrogen deprivation and/or anti-estrogen treatment. Paper-14487855.
PURPOSE: To evaluate the effect of corneal epithelial wounding on lacrimal gland expression of hepatocyte growth factor ( HGF), keratinocyte growth factor ( KGF), and epidermal growth factor ( EGF) in the rabbit model. Paper-1965486.
In contrast to rat cells, human hepatocytes consistently failed to respond to KGF, aFGF, or bFGF with or without heparin, under conditions where EGF and HGF stimulated DNA synthesis up to sixfold. Paper-8221377.
Messenger RNA (mRNA) levels of interleukin-1 (IL-1)alpha, IL-1beta, tumor necrosis factor alpha ( TNF-alpha), keratinocyte growth factor ( KGF), and procollagen-1 were analyzed by real-time reverse transcription-polymerase chain reaction. Paper-13350697.
Papillary and reticular fibroblasts grown in monolayer culture differed significantly from each other in their release of keratinocyte growth factor ( KGF) and granulocyte-macrophage colony stimulating factor ( GM-CSF) into culture medium. Paper-10278135.
The mRNA expressions of HGF, HGFR, EGF, VEGF, and COX-2, but not EGFR, KGF, KGFR, bFGF, and COX-1, were significantly increased in the injured mucosa of EE patients compared with those of normal mucosa ( P < 0.05). Paper-12895503.
Both KGF and HGF stimulated thymidine uptake in the cell line when cultured for 2 d in serum-free conditions; the growth increase was magnified when tumor necrosis factor (TNF)-alpha was also added to the cultures. Paper-8437747.
Buccal fibroblasts were stimulated with different concentrations of ST extracts in a double dilution from 0.50% w/v to 0.03% w/v. Supernatant was collected after 24/48/72/96 h and assayed for HGF, KGF, and GM-CSF by ELISA. Paper-10734676.
Differential expression analysis by gene array of cell cycle modulators in human corneal epithelial cells stimulated with epidermal growth factor ( EGF), hepatocyte growth factor ( HGF), or keratinocyte growth factor ( KGF). Paper-8898807.
PURPOSE: To examine the expression and function of hepatocyte growth factor ( HGF), keratinocyte growth factor ( KGF), epidermal growth factor ( EGF) and other growth factor-cytokine-receptor systems in lens epithelial cells. Paper-1094126.
KGF stimulated fluid secretion equally in lung buds from cystic fibrosis transmembrane conductance regulators ( CFTR) -/- and wild-type embryos, indicating that the effects were mediated by CFTR-independent Cl- transport. Paper-853092.
These novel results show that HGF and KGF are active in the alveolar space early in ALI, probably mediating early events in lung repair, and that increased levels of HGF in edema fluid may have prognostic value early in ALI. Paper-1531106.
We sequenced the coding regions and performed association testing on 12 genes ( FGFR1, FGFR2, FGFR3, FGF2, FGF3, FGF4, FGF7, FGF8, FGF9, FGF10, FGF18, and NUDT6) and used protein structure analyses to predict the function of amino acid variants. Paper-13153989.
We have previously shown that keratinocyte growth factor ( KGF) enhances the motility of estrogen receptor (ER)-positive breast cancer cells and that this motility response is associated with cellular levels of the KGF receptor (KGFR). Paper-10115361.
Expression of KGF was increased in the KGF group compared to all other groups and was associated with an increased synthesis of SPB by alveolar cells and an ectopic synthesis of SPB by bronchiolar cells compared to TO treatment alone. Paper-15089895.
In addition, cultured human keratinocytes were grown in medium containing IL-1alpha or KGF at concentrations of 0, 20, and 100 ng/mL, and the differences in the expression of cdk2 and cdk4 were investigated and compared by Western blot analysis. Paper-1913781.
Keratinocyte growth factor ( KGF) and fibroblast growth factor 10 ( FGF-10) are stromal-derived growth factors that interact with their epithelial FGFR2 receptors to mediate stromal--epithelial cell interaction within the prostate gland. Paper-12625525.
SBR did not affect small bowel or colonic goblet cell number or TFF3 expression; however, goblet cell number and TFF3 expression in both small bowel and colon were markedly up-regulated by KGF treatment and unaffected by GLP-2 and GH. Paper-11580029.
The expression patterns for 8 of 15 genes have been reported previously in prostate tissues (TGFbeta3, TGFBR3, IGFII, IGFBP2, VEGF, FGF7, ERBB3, MYC), but those of seven genes are reported here for the first time (MLH1, CYP1B1, RFC4, EPHB3, MGST1, BTEB2, MLP). Paper-8767778.
Our aim was to compare effects of a human glucagon-like peptide-2 (GLP-2) analog, recombinant growth hormone ( GH) and recombinant keratinocyte growth factor ( KGF) on jejunal, ileal, and colonic growth and functional indices after 80% SBR in rats. Paper-11580029.
The effects exerted by the keratinocyte growth factor ( KGF) on intestinal epithelial cells cultured in vitro are influenced by cell confluence and differentiation through the modulation of keratinocyte growth factor receptor ( KGFR) expression. Paper-13748741.
Furthermore we determined the time- and dose-dependency of the incubation with salivary leptin on the production of epidermal growth factor ( EGF) and keratinocyte growth factor ( KGF), which are growth factors relevant to keratinocyte proliferation. Paper-10771612.
Angiogenin, Ang2, fibroblast growth factor basic, intercellular adhesion molecule (ICAM)-1, keratinocyte growth factor ( KGF), platelet-derived growth factor-BB, and VEGF-A were measured using a FASTQuant angiogenic growth factor multiplex protein assay. Paper-12200252.
Our results demonstrate for the first time that KGF/ FGF-7 induces NF-kappaB activation and that NF-kappaB plays an essential role in regulation of KGF/FGF-7-inducible gene expression and KGF/ FGF-7-initiated cellular responses. Paper-13125236.
We have screened normal oral mucosa and oral squamous cell carcinoma ( SCC) for expression of bFGF by immunohistology and northern analysis and used RT-PCR to look for transcripts for KGF and the high-affinity FGF receptors FGFR1 and FGFR2. Paper-660613.
CONCLUSION: It can be surmised that, in cholesteatoma, accelerated expression of IL-1alpha and KGF by inflammatory cells at subepithelial sites of inflammation leads to upregulation of cdk2 and cdk4 in epithelial cells and to cell proliferation. Paper-1913781.
Due to the important functions of FGF-7 and FGF-10 for repair of injured epithelia, our findings suggest that upregulation of FGF-BP expression after injury stimulates FGF activity at the wound site, thus enhancing the process of epithelial repair. Paper-11033255.
Corneal epithelial wound healing is intimately controlled by a variety of growth factors, such as epidermal growth factor ( EGF), keratinocyte growth factor ( KGF), hepatocyte growth factor ( HGF), and transforming growth factor-betas (TGF-betas). Paper-1330166.
Recent studies demonstrated the expression of the GLP-2 receptor on fibroblasts located in the subepithelial tissue, where it might induce the release of growth factors such as keratinocyte growth factor ( KGF) or vascular endothelial growth factor ( VEGF). Paper-12675196.
In telomere-elongated cells prepared by transfection with human telomerase reverse transcriptase cDNA, high expression levels of these two genes were maintained, suggesting a causal relation between telomere shortening and reduced expression of KGF and IGF-II. Paper-9554555.
In this study, pigs were ovariectomized and treated daily with P(4) to assess effects of 40 days of P(4) exposure on SPP1, P(4) receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 ( FGF7) expression in porcine endometria. Paper-15420246.
Induction of TGF alpha in KGF-treated keratinocytes, coupled to activation and down-modulation of the EGFR, suggests that TGF alpha may be a proximal effector of KGF action for at least certain aspects of epidermal growth and differentiation. Paper-190436.
In the present study, keratinocyte growth factor ( KGF) significantly stimulated proliferation of gastric epithelial cells dose dependently and synergistically with hepatocyte growth factor ( HGF), epidermal growth factor ( EGF) and insulin. Paper-924858.
Three polypeptide growth factor, IGF-I, keratinocyte growth factor ( KGF), and EGF, stimulated AR-mediated reporter-gene transcription in the absence of androgen in DU-145 cells, which were cotransfected with the reporter gene and an AR expression vector. Paper-497600.
The factors regulating human fetal lung liquid secretion are poorly understood; however, recent studies in murine models show that keratinocyte growth factor ( KGF, FGF-7) and fibroblast growth factor 10 ( FGF-10) stimulate liquid secretion. Paper-2031831.
Cortactin phosphorylation induced by both growth factors was Src-dependent, while its membrane translocation and cell migration were blocked by either Src and PI3K inhibitors, suggesting that both pathways are involved in KGF- and FGF10-dependent motility. Paper-13210729.
Within this concentration range, FGF-10 did not stimulate DNA synthesis in NIH/3T3 mouse fibroblasts. rFGF-10 bound the KGFR with high affinity comparable to that of KGF, and did not bind detectably to either the FGFR1c (Flg) or FGFR2c (Bek) receptor isoforms. Paper-1453860.
These data indicate that SREBP-1c is required for the stimulation of lipogenesis by KGF in the alveolar type II cells and is a key regulator of lung lipid metabolism and that expression of SREBP-1c is sufficient to induce lipogenesis in rat type II cells. Paper-12066521.
Cultured HPDE cells express low levels of epidermal growth factor receptor ( EGFR), erbB2, transforming growth factor (TGF)-alpha, Met/ hepatocyte growth factor receptor ( HGFR), vascular endothelial growth factor ( VEGF), and keratinocyte growth factor ( KGF). Paper-1485773.
This regulatory process is initiated by interleukin-1 (IL-1) release in keratinocytes, which induces expression of keratinocyte growth factor ( KGF/ FGF-7) and granulocyte macrophage-colony stimulating factor ( GM-CSF) in fibroblasts. Paper-9925265.
Furthermore, combining KGF transfection and TO resulted not only (as did TO alone) in the correction of the CDH-associated lung hypoplasia and decreased RAC, but also in increased SPB synthesis, suggesting a better maturation of the re-growing lung (compared to TO alone). Paper-15089895.
At 24-hr incubations, KGF showed a greater capacity than did EGF and HGF to stimulate microsomal 12-LOX activity, while at 48 hr 12(S)-HETE synthesis was significantly greater in EGF-treated cells as compared to that of HGF- and KGF-treated cells. Paper-9668875.
These include epidermal growth factor ( EGF), fibroblast growth factor ( FGF), insulin-like growth factor (IGF), keratinocyte growth factor ( KGF), platelet-derived growth factor (PDGF), transforming growth factor (TGF) and vascular endothelial growth factor ( VEGF). Paper-10172861.
Fibroblast growth factor ( FGF) 10 and FGF7 both decrease Foxf1 expression and we speculate that this is mediated by transcriptional activation of epithelial Bmp4 (in the case of FGF10) and by inhibition of Shh expression for FGF7. Paper-8851479.
KGF signaling in TECs activates both the p53 and NF-kappaB pathways and results in the transcription of several target genes necessary for TEC function and T-cell development, including bone morphogenetic protein 2 ( BMP2), BMP4, Wnt5b, and Wnt10b. Paper-13189296.
Locally produced factors that promote the primordial to primary follicle transition include growth factors such as kit ligand (KL), leukaemia inhibitory factor ( LIF), bone morphogenic proteins (BMP's), keratinocyte growth factor ( KGF) and basic fibroblast growth factor ( bFGF). Paper-11587134.
Cell lysates of corneal epithelial cells in rabbits stimulated with keratinocyte growth factor ( KGF) and hepatocyte growth factor ( HGF) were processed for Western blot using antibodies to phosphorylated p44/42 MAPK. Paper-10040317.
Production of the 92-kD enzyme was substantially increased in the presence of epidermal growth factor ( EGF) and hepatocyte growth factor ( HGF) as compared to control but not in the presence of insulin-like growth factor-1 (IGF-1) or keratinocyte growth factor ( KGF). Paper-698135.
KGF stimulates lipogenic enzymes, including fatty acid synthase and stearyl-CoA desaturase-1, and transcription factors involved in lipogenesis, such as sterol regulatory element binding protein (SREBP)-1c and CCAAT/enhancer binding protein (C/EBP)alpha and C/EBPdelta. Paper-12066521.
The mRNA expression levels of KIT ligand ( KITLG), KIT (KIT), growth differentiation factor 9 ( GDF9), and bone morphogenetic protein 15 ( BMP15) in the cultured COCGs were determined in FGF7-treated (10 ng/ml) cultures using real time RT-PCR analysis. Paper-13015479.
Immunofluorescence confocal microscopy using endocytic markers as well as tumor susceptibility gene 101 ( TSG101) silencing demonstrated that KGF drives KGFR to the degradative pathway, while FGF10 targets the receptor to the recycling endosomes. Paper-12601885.
METHODS: Archived human skin biopsies (24 SLs and 14 healthy skins) were studied using immunohistochemistry for KGF/ KGFR, proliferation marker Ki67, stem cell marker keratin-15 ( K15), tyrosinase ( TYR), stem cell factor (SCF), and protease-activated receptor-2 (PAR-2). Paper-15290406.
In this report, we show that KGF/ FGF-7 activated nuclear factor kappaB ( NF-kappaB), which in turn induced expression of VEGF, MMP-9, and urokinase-type plasminogen activator and increased migration and invasion of KGF/ FGF-7-stimulated human pancreatic ductal epithelial cells. Paper-13125236.
K-sam generates several variant transcripts by alternative splicing, and the most abundant K-sam transcript in KATO-III cells was cloned as the K-sam-IIC3 cDNA, which has the KGF-binding motif and a short carboxyl terminus lacking a putative phospholipase C-gamma 1 association site, Tyr-769. Paper-268108.
KGF stimulated the expression of the transcription factors sterol-regulatory element binding protein-1 ( SREBP-1), CCAAT/enhancer binding protein alpha (C/EBPalpha), and C/EBPdelta and two key enzymes involved in lipogenesis, FAS and stearoyl coenzyme A desaturase-1 ( SCD-1). Paper-11117591.
OBJECTIVES: To determine bronchoalveolar lavage (BAL) fluid concentrations of keratinocyte growth factor ( KGF) and hepatocyte growth factor ( HGF), two potent growth factors for alveolar type II epithelial cells, in patients with acute respiratory distress syndrome (ARDS). Paper-8358995.
BACKGROUND: Keratinocyte growth factor ( KGF) has been shown to induce breast cancer metastasis in animal models. cDNA microarrays have revealed that KGF increased Wilms tumor 1 ( WT1) and focal adhesion kinase (FAK) expression in breast cancer cells. Paper-14303468.
Hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF) are among the most potent mitogens identified for alveolar type II epithelial cells and may have other important functions in repair of the alveolar epithelium in acute lung injury (ALI). Paper-1531106.
Reverse transcriptase-polymerase chain reaction analysis revealed KGF mRNA expression in the mesenchymal component of the 13-day embryonic lung, while message for KGF receptor ( KGFR) was expressed in the epithelium, confirming the paracrine nature of KGF/ KGFR axis. Paper-749771.
To determine the involvement of PI3K/Akt pathway in the survival effect of KGF, the growth rate of MCF-7 cell was measured by non-radioactive cell proliferation assay after treatments of KGF, LY, 4-OH-Tam, KGF with LY, KGF with LY and 4-OH-Tam, or vehicles as control for 3 days. Paper-13918227.
Platelet-derived growth factor (PDGF), epidermal growth factor ( EGF) and alpha fibroblastic growth factor (alphaFGF) all promoted cell proliferation and were chemokinetic to dermal fibroblasts, but not keratinocyte growth factor ( KGF) or transforming growth factor beta ( TGFbeta). Paper-11278464.
We studied the effect of KGF on LPS and GalN- induced hepatic failure in vivo and on TNF- and ActD-induced hepatocyte apoptosis in vitro, where it was compared with those of hepatic growth factor ( HGF) and epidermal growth factor ( EGF). Paper-1458582.
Recent study has revealed that this difference signifies a differential ligand affinity; the receptor encoded by the K-sam-I cDNA has a high affinity for basic fibroblast growth factor ( bFGF), while the K-sam-II cDNA corresponds to a receptor with the high affinity for keratinocyte growth factor ( KGF). Paper-7921717.
The differential effects of GLP-2, GH and KGF administration in this model of short bowel syndrome suggest that individual therapy with these growth factors may not be an adequate strategy to maximally improve adaptive gut mucosal growth and cytoprotection after massive small intestinal resection. Paper-11580029.
These findings suggested that a selective COX-2 inhibitor, JTE-522, downregulates KGF production from gastric fibroblasts, resulting in the inhibition of paracrine epithelial-mesenchymal interactions of proliferation between scirrhous gastric cancer cells and gastric fibroblasts. Paper-12372487.
Quantitative immunofluorescence microscopy and biochemical analysis showed that both overexpression and siRNA interference of Hrs inhibit the KGF- triggered KGFR degradation, blocking receptor transport to lysosomes and causing its rapid reappearance at the plasma membrane. Paper-13838023.
The use of KGF-HFc in immunofluorescence and in immunogold electron microscopy on KGFR transfectants, A253 epithelial tumor cells and human cultured keratinocytes allowed us to follow the early steps of KGF internalization and revealed that this process occurred through clathrin-coated pits. Paper-1718942.
Explants incubated with antisense oligonucleotides targeted to the initiation site of translation of both the splice variants of the fibroblast growth factor receptor-2 ( FGFR2) gene, KGFR and bek, exhibited a similar reduction in lung branching as observed with antisense KGF oligonucleotides. Paper-749771.
The data indicate that Has2 and Has3 belong to the targets of KGF in keratinocytes, and support the idea that enhanced hyaluronan synthesis acts an effector for the migratory response of keratinocytes in wound healing, whereas it may delay keratinocyte terminal differentiation. Paper-10043138.
Both growth factors promoted phosphorylation and subsequent membrane translocation of cortactin, an F-actin binding protein involved in cell migration; however, FGF10- induced cortactin phosphorylation was reduced, more transient and delayed with respect to that promoted by KGF. Paper-13210729.
The AP-1 transcription factor subunit JUN plays a crucial role in this mesenchymal-epithelial interplay by regulating the expression of two critical paracrine-acting cytokines, keratinocyte growth factor ( KGF) and granulocyte-macrophage colony-stimulating factor ( GM-CSF). Paper-10780869.
Use of anti- KGF blocking antibodies and of a tyrosine kinase KGFR inhibitor demonstrated the contribution of KGF and the direct role of its receptor in the regulation of epithelial growth and differentiation, indicating that KGF is a crucial paracrine factor involved in promoting these effects. Paper-13748741.
These include the use of cytokines such as IL-7, IL-12 and IL-15; growth factors and hormones like keratinocyte growth factor ( KGF), insulin-like growth factor (IGF)-1 and growth hormone ( GH); adoptive transfer of ex vivo-generated precursor T cells (pre-T) and sex steroid ablation (SSA). Paper-16228054.
In the present study, we show that keratinocyte migration induced by TGF-beta1, KGF, EGF, TGF-alpha and staurosporine depends on EGFR signaling, involves autocrine HB-EGF expression and is potently blocked by GSK-3 inhibitors SB-415286 and LiCl. Paper-12193442.
Bronchoalveolar lavage fluid (BALF) was obtained from ventilated preterm infants (n = 62) on postnatal d 0, 1, 3, and 7 and analyzed for total phospholipids (PL), VEGF, PDGF-BB, TGF-alpha and -beta1, granulocyte macrophage colony stimulating factor ( GM-CSF), and keratinocyte growth factor ( KGF). Paper-14159365.
Although transcripts for ClC-1 and ClC-7 Cl(-) channels increased, KGF failed to augment transepithelial Cl(-) transport in CF epithelia, suggesting that KGF- stimulated Cl(-) transport in differentiated airway epithelia depends on the CFTR Cl(-) channel. Paper-8882346.
MATERIALS AND METHODS: The K-sam gene encoding the receptor for keratinocyte growth factor ( KGF) was transduced to three factor-dependent hematopoietic cell lines ( Ba/F3, 32Dcl3, and UT-7/GM) using retroviral vector, and their proliferation, differentiation, and intracellular signaling were studied. Paper-9202678.
The effects of heparan sulfate proteoglycan ( HSPG) on binding and signaling by acidic FGF ( aFGF) and KGF via the KGFR were studied using surface- bound and soluble receptor isoforms expressed in wild type and mutant Chinese hamster ovary (CHO) cells lacking HSPG. Paper-1741945.
The recycling endocytic pathway followed by KGFR upon FGF10 stimulation correlates with the higher mitogenic activity exerted by this ligand on epithelial cells compared with KGF, suggesting that the two ligands may play different functional roles through the regulation of the receptor endocytic transport. Paper-12601885.
Few studies, however, have focused on the possible involvement of the keratinocyte growth factor ( KGF/ FGF-7) and the fibroblast growth factor 10 ( FGF-10/KGF-2), which are secreted by fibroblasts and stimulate keratinocyte proliferation acting through a receptor specifically expressed by epithelial cells. Paper-10786781.
Ovaries from 4-day-old rats were placed into organ culture and incubated in the absence or presence of AMH, either alone or in combination with known stimulators of follicle transition, including basic fibroblast growth factor ( bFGF), kit ligand (KITL), or keratinocyte growth factor ( KGF). Paper-13357279.
An enzyme-linked immunosorbent assay ( ELISA) was used to assay growth factors protein levels for epidermal growth factor, basic fibroblast growth factor ( bFGF), hepatocyte growth factor ( HGF), keratinocyte growth factor ( KGF), transforming growth factor beta1 (TFG-β(1) ) and nerve growth factor (NGF). Paper-15444897.
All fetuses were euthanized at 132 days of gestation and various analytical studies [lung weight, radial alveolar count (RAC), KGF and surfactant protein B ( SPB) expression, number of ATII cells] were performed to assess the efficiency of KGF transfection and its effects on fetal lung development. Paper-15089895.
OBJECTIVE: This study was designed to evaluate the effect of growth factors such as epidermal growth factor ( EGF), transforming growth factor alpha ( TGF-alpha), basic fibroblast growth factor ( bFGF) and keratinocyte growth factor ( KGF) on cell proliferation and lipogenesis in cultured hamster sebocytes. Paper-9425187.
Effects of KGF on cell morphology, expression of surfactant apoproteins A, B, and C (SP-A, -B, and -C), and expression of aquaporin 5 ( AQP5), a water channel present in situ on the apical surface of alveolar type I (AT1) cells but not expressed in alveolar type II (AT2) cells, were evaluated in AECs grown in primary culture. Paper-1388753.
KGF-treated recipients had elevated serum levels of the Th2 cytokine interleukin 13 ( IL-13) on day 6 after T-cell transfer concomitant with reduced levels of the inflammatory cytokines tumor necrosis factor-alpha ( TNF-alpha) and interferon gamma ( IFN-gamma). Paper-8636736.
Wounds were examined by transmission electron microscopy, the detection of free 3'-OH DNA ends and immunohistochemistry of proliferating cell nuclear antigen ( PCNA), inducible nitric oxide synthase ( iNOS), keratinocyte growth factor ( KGF) and keratinocyte growth factor receptor ( KGFR). Paper-9384271.
Western blot analysis of cultured corneal epithelial cells in rabbits showed phosphorylation of p44/42 MAPK after 30 minutes in response to KGF and HGF, whereas non-activated p44/42 MAPK was ordinarily detected even at the absence of KGF or HGF. Paper-10040317.
RESULTS: KGF cDNA increased insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 ( IGFBP-3), and fibroblast growth factor ( FGF), decreased transforming growth factor-beta ( TGF-beta), while it had no effect on platelet-derived growth factor (PDGF) levels in the wound. Paper-13184920.
Pretreatment with KGF at 48 hr protected against bleomycin- induced alterations in pulmonary physiology and increased surfactant protein C-positive (SP-C)-positive cells and SP-A, SP-B, SP-C, and SP-D mRNA levels after bleomycin instillation when compared to saline pretreated rats on day 1 or day 7. Paper-1049043.
In a model of corneal wound healing, we found that two paracrine growth factors, hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF), induced rapid and marked activation and prompt nuclear accumulation of phospho- p38 (p-p38) and -ERK1/2 (p- ERK1/2), but not of JNK (p-JNK1/2), in corneal epithelial cells. Paper-9661257.
Blood gammadelta T cells isolated from healthy donors were grown in the presence of isopentenyl pyrophosphate (IPP) or transforming growth factor-beta1 (TGF-beta1)/interleukin-15 (IL-15) for 24 h and were assessed for the expression and synthesis of FGF-9, keratinocyte growth factor ( KGF), and epidermal growth factor ( EGF). Paper-10402503.
To test the functionality of these DS, we measured ( ELISA) the stimulatory effect on HDF in the matrix, of serial dilutions of human serum (HS) on the production of wound healing mediators: interleukin-8 ( IL-8), vascular endothelial growth factor ( VEGF), keratinocyte growth factor ( KGF) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Paper-9894069.
CONCLUSIONS: Our results demonstrate that the vitamin D analogue BXL-628 is able to suppress KGF- induced proliferation and invasion of AI- PC cells in vitro, prospecting a possible use of the drug, which is currently in phase II clinical studies for benign prostatic hyperplasia, in the treatment of advanced prostate cancer. Paper-11367588.
CONCLUSIONS: These results demonstrate that p44/42 MAPK is activated during the corneal wound healing process and suggest that KGF and HGF play an important role in initiation of cell migration and proliferation in the initial wound healing process by activating p44/42 MAPK. Paper-10040317.
As part of a study of elicited angiogenesis, hyaluronan (HA)-based hydrogels crosslinked by polyethylene glycol diacrylate (PEGDA) were loaded with combinations of the cytokine growth factors vascular endothelial growth factor ( VEGF), angiopoietin-1 ( Ang-1), keratinocyte growth factor ( KGF) and platelet-derived growth factor ( PDGF). Paper-15232877.
PURPOSE: To determine whether interleukin 1 beta ( IL-1 beta) messenger RNA (mRNA) and protein were expressed in corneal cells and to examine the effects of IL-1 alpha and IL-1 beta on the expression of hepatocyte growth factor ( HGF) and keratinocyte growth factor ( KGF) mRNAs and proteins in corneal stromal fibroblasts. Paper-1115728.
In this study the expression of the FGFs keratinocyte growth factor ( KGF), acidic FGF ( aFGF) and basic FGF ( bFGF) was examined in tumour tissue specimens from 14 patients with advanced-stage squamous cell carcinoma of the head and neck (SCCHN) and 3 SCC cell lines by reverse-transcribed polymerase chain reaction ( RT-PCR) and immunohistochemistry. Paper-1566867.
Biochemical analysis showed that KGFR is ubiquitinated and degraded after KGF treatment but not after FGF10 treatment, and that the alternative fate of KGFR might depend on the different ability of the receptor to phosphorylate the fibroblast growth factor receptor substrate 2 ( FRS2) substrate and to recruit the ubiquitin ligase c-Cbl. Paper-12601885.
Western blot analysis with anti- FGFR2 and anti-phosphotyrosine antibodies, as well as parallel double immunofluorescence and confocal analysis of NIH3T3 KGFR transfectants treated with KGF at 4 degrees C, followed by incubations at 37 degrees C for different time points, showed that KGF induced endocytosis of tyrosine activated KGFRs. Paper-1718942.
Fusion of FGF7 at the N-terminus with glutathione-S-transferase ( GST) followed by removal of GST by proteolysis while bound to natural ligand heparin improved the intrinsically low yields from Escherichia coli hosts to 3.2 mg per liter per OD(600), which was still only 10% of that for FGF1. Paper-10552343.
The goals of this study were to determine: (1) whether HGF and KGF are present in pulmonary edema fluid from patients with ALI and control patients with hydrostatic pulmonary edema; (2) whether HGF and KGF are biologically active in pulmonary edema; and (3) whether HGF or KGF levels are associated with clinical outcome. Paper-1531106.
The keratinocyte growth factor receptor or fibroblast growth factor receptor 2b ( KGFR/FGFR2b) is activated by the specific interaction with the keratinocyte growth factor ( KGF/ FGF7), which targets the receptor to the degradative pathway, and the fibroblast growth factor 10 ( FGF10/KGF2), which drives the receptor to the juxtanuclear recycling route. Paper-13838023.
These synonyms are used for gene FGF7 (fibroblast growth factor 7): KGF, Keratinocyte growth factor, Heparin-binding growth factor 7, HBGF-7, Fibroblast growth factor 7, FGF-7.
These accession numbers are used for gene FGF7: CR609737 (NCBI_GENBANK__AC), CR542002 (NCBI_GENBANK__AC).
FGF7 is a homologue of FGF7 (fibroblast growth factor 7) from Pan troglodytes.
FGF7 is a homologue of FGF7 (fibroblast growth factor 7) from Canis lupus familiaris.
FGF7 is a homologue of FGF7 (fibroblast growth factor 7) from Bos taurus.
FGF7 is a homologue of FGF7 (fibroblast growth factor 7) from Gallus gallus.
FGF7 is a homologue of Fgf7 (fibroblast growth factor 7) from Mus musculus.
FGF7 is a homologue of Fgf7 (fibroblast growth factor 7) from Rattus norvegicus.
FGF7 is a homologue of fgf7 (fibroblast growth factor 7) from Danio rerio.
Important links !
iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.