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The binding of this variant HRG to plasminogen was normal. Paper-7820690.
Soluble HPRG did not significantly influence plasminogen activation. Paper-946808.
Complex formation of platelet thrombospondin with histidine-rich glycoprotein. Paper-4759299.
Fluctuations of HRG caused only minor changes in free plasminogen concentrations. Paper-4598471.
In addition, HRG was able to displace biologically active bFGF from the ECM. Paper-139834.
HRG in plasma behaved as a uniform fraction with respect to plasminogen binding. Paper-6131183.
Importantly, plasminogen interacted specifically with GAG- bound HPRG. Paper-1360803.
Plasma histidine-rich glycoprotein and plasminogen in patients with liver disease. Paper-4933441.
HRG had inhibitory effects on DS as well, as was observed from assaying HC II activity. Paper-4989012.
Both clusterin and HRG are regulators of the membrane attack complex (C5b-C9) of complement. Paper-473049.
We found that binding of HRGP to TSP-1 was similarly mediated by TSP type I repeats. Paper-8689729.
Histidine-rich glycoprotein and plasminogen plasma levels in term and preterm newborns. Paper-6686994.
Modulation of heparin cofactor II activity by histidine-rich glycoprotein and platelet factor 4. Paper-4988137.
Their results show that HRG, like PF 4, has an affinity, not only for heparin, but also for DS. Paper-7331981.
In contrast, HPRG abrogates the stimulatory effects of fibrinogen on Plg activation in solution. Paper-10911023.
Histidine-rich glycoprotein inhibits the antiangiogenic effect of thrombospondin-1. Paper-8689729.
Effects of histidine-proline-rich glycoprotein on plasminogen activation in solution and on surfaces. Paper-10911023.
The heparin-neutralizing ability of HRG in the thrombin inhibition by PCI was not affected by Ca2+. Paper-7523895.
The newborns had significantly lower plasminogen and HRG levels when compared with those of the adults. Paper-6686994.
No species differences were found, as either rabbit or human HPRG bound readily to rabbit or human PLG. Paper-172840.
The effect of HRG caused the level of free plasminogen to be only 50-60% of the level of total plasminogen. Paper-4598471.
ERK activation remained elevated for 2 h following high doses of HRG which induce differentiation. Paper-1577501.
Furthermore, truncated HRGP, devoid of the heparin-binding and histidine-rich domain, was not antibacterial. Paper-12428358.
The data indicate a close link among HRG-beta stimulation, HER signaling, and AP-1 activation. Paper-13051552.
The unique features present in the cloned cDNA indicate that p43 is a novel member of the HRGP family of proteins. Paper-1959727.
Etiolated seedlings subjected to a pulse of red light accumulated PvPRP1, GRP, and HRGP mRNAs in the hypocotyls. Paper-11716321.
Association of purified skeletal-muscle AMP deaminase with a histidine-proline-rich-glycoprotein-like molecule. Paper-1171373.
We have recently reported the association of an HPRG-like protein with rabbit skeletal muscle AMP deaminase ( AMPD). Paper-1718190.
In severe (Child C) cirrhosis, HRG level, total plasminogen level, and free plasminogen level were all decreased. Paper-6307177.
HPRG also augments the increase in Plg activation caused by fibrinogen fragments either in solution or on GAG surfaces. Paper-10911023.
However, free plasminogen not bound to histidine-rich glycoprotein was not significantly different between these two groups. Paper-5903091.
These mRNA changes include differential regulation of transcripts derived from the GRP and HRGP multigene families. Paper-11716321.
The formation of the thrombospondin- histidine-rich glycoprotein complex was specific, concentration dependent, and saturable. Paper-4759299.
In HRG-alpha the N-terminal residues 2-6 form a well-defined beta strand rather than being disordered as found for hEGF. Paper-555501.
Three members of the human cystatin gene superfamily, AHSG, HRG, and KNG, map within one megabase of genomic DNA at 3q27. Paper-220647.
Levels of thrombospondin-4 (900%; p < 0.001) and alpha2-macroglobulin (300%, p < 0.05) mRNA increased substantially in the HRG. Paper-11443917.
This was associated with significant increases in plasma plasminogen and a concomitant reduction in histidine-rich glycoprotein. Paper-4618902.
Other newly discovered modulators of the fibrinolytic system include histidine-rich glycoprotein, tetranectin and thrombospondin. Paper-5398889.
The biological activity of partially purified rat NDF/ HRG was examined and found to be the same as that of the pure growth factor. Paper-514890.
In fact, HRG is 10 times less effective than PF 4 in neutralizing the 50% antithrombin activity of HC II in the presence of DS. Paper-7331981.
In contrast, a transient 5 min peak of ERK activation in response to doses of HRG which induce proliferation was observed. Paper-1577501.
We conclude that histidine-rich glycoprotein and platelet factor 4 can regulate the antithrombin activity of heparin cofactor II. Paper-4988137.
No correlation between HRGP level and t-PA- mediated plasminogen activation was observed.(ABSTRACT TRUNCATED AT 250 WORDS) Paper-7862153.
The interactions between the streptococcal protein and the plasma proteins were further characterized using purified clusterin and HRG. Paper-473049.
This investigation will be continued to clarify the role of HRG, HC II, AT III, and heparin in the prevention of thrombi formation. Paper-4989012.
Acceleration of plasminogen activation by tissue plasminogen activator on surface- bound histidine-proline-rich glycoprotein. Paper-946808.
Familial association of high levels of histidine-rich glycoprotein and plasminogen activator inhibitor-1 with venous thromboembolism. Paper-7862153.
The ternary complex model implies that, HRG being responsible for efficient G protein activation, it should be as stable as possible. Paper-10490383.
Histidine-proline-rich glycoprotein ( HPRG), also known as histidine-rich glycoprotein, is a major plasminogen-binding protein. Paper-946808.
Platelet factor 4 abolishes the activation of heparin cofactor II by dermatan sulfate, but plasma histidine-rich glycoprotein does not. Paper-11924208.
Heparin cofactor II, histidine-rich glycoprotein, alpha 2-macroglobulin, pre-kallikrein and factor XII had no discriminative power. Paper-6740051.
Although fibrinogen, plasminogen, and alpha 2-antiplasmin were present in culture supernatants, histidine-rich glycoprotein was not detected. Paper-4271120.
Familial elevation of plasma histidine-rich glycoprotein. A case associated with recurrent venous thrombosis and high PAI-1 levels. Paper-7495610.
HRGP mRNAs are abundant in outer and inner phloem regions, while GRP mRNAs are present mostly in primary xylem and in the cambium region. Paper-89993.
Histidine-rich glycoprotein binds to DNA and Fc gamma RI and potentiates the ingestion of apoptotic cells by macrophages. Paper-9579646.
Interaction of histidine-proline-rich glycoprotein with plasminogen: effect of ligands, pH, ionic strength, and chemical modification. Paper-172840.
In contrast to HRG, there was no significant difference in the heparin-neutralizing ability of PF4 in the presence or absence of 1 mM Ca2+. Paper-7523895.
In this work we characterized extensively the circumstances under which HPRG accelerates plasminogen activation and the specificity of this effect. Paper-946808.
The immobilized N- and C-terminal domains, but not the histidine-proline-rich domain of HPRG, also bound plasminogen and stimulated its activation. Paper-946808.
At saturation the relative molar stoichiometry of Plg:TPA was 3:1 within the TSP-containing complexes and 1:1 within HRGP-containing complexes. Paper-5126593.
Of the ligands of HPRG tested, mesoheme (20 microM) but not heparin (M(r) 10,000, 10 microM) inhibits the formation of the HPRG- PLG complex. Paper-172840.
The hormone group showed an increase in plasminogen level to about 150% of normal, while the concentration of HRG was decreased to about 75% of normal. Paper-4117132.
In fetuses, euglobulin clot lysis time (ECLT) was significantly shortened, plasminogen level was low and histidine-rich glycoprotein undetectable. Paper-7103045.
Modified crossed immunoelectrophoresis to study with whole plasma the reversible complex formation of histidine-rich glycoprotein with plasminogen. Paper-6131183.
Decreased levels of histidine-rich glycoprotein ( HRG) and increased levels of free plasminogen in women on oral contraceptives low in estrogen. Paper-4117132.
The authors studied the interaction of DS and low molecular weight DS, in a purified system with HRG, platelet factor 4 ( PF 4), and with HC II. Paper-7331981.
A sequential study of plasma histidine-rich glycoprotein and plasminogen in patients with acute myocardial infarction and deep vein thrombosis. Paper-4598471.
Evidence for the interaction between (-)-epigallocatechin gallate and human plasma proteins fibronectin, fibrinogen, and histidine-rich glycoprotein. Paper-878748.
Histidine-rich glycoprotein inhibits the antithrombin activity of heparin cofactor II in the presence of heparin or dermatan sulfate. Paper-4988338.
Plasminogen binding by alpha 2-antiplasmin and histidine-rich glycoprotein does not inhibit plasminogen activation at the surface of fibrin. Paper-7453301.
Western blot analysis showed that complement C8, C9, factor D, and S-protein in diluted serum were bound by nylon membrane-immobilized HRG. Paper-60940.
Locations of the introns in HRG gene coding for cystatin domains are essentially identical with those of cystatin SN, SA and C, and kininogen genes. Paper-5801313.
TIE1 and TIE2 mRNA levels were unaltered in the LRG, whereas transcription levels of both genes were increased by 2.5-fold in the HRG (p < 0.01). Paper-11443917.
Clexane (M(r) = 4,500) also bound antithrombin III, but both histidine-rich glycoprotein and vitronectin were quantitatively significant neutralising proteins. Paper-7820698.
P123 did not cross- link to plasminogen, histidine-rich glycoprotein, fibronectin, or plasma globulins other than fibrinogen/fibrin. Paper-1708261.
Effects of zinc and calcium ions on the heparin-neutralizing abilities of histidine-rich glycoprotein ( HRG) and platelet factor 4 ( PF4) were examined. Paper-7523895.
The concentration of serum HRG decreased during the last trimester of pregnancy reaching a nadir at the 36-37th week ( HRG 49 +/- 14 g/l, mean +/- SD). Paper-4359302.
In blood drawn into streptokinase or into urokinase, HRG (78 kDa) was degraded producing peptides ranging in apparent molecular weight from 67 to 9 kDa. Paper-5100843.
Histidine-proline-rich glycoprotein ( HPRG) is an abundant multidomain plasma protein evolutionarily related to high-molecular-weight kininogen. Paper-9552588.
The characteristic sequence motifs of hydroxyproline-rich glyco-proteins ( HRGP) are present in the cDNA corres-ponding to the N-terminal domain of the mature p43. Paper-1959727.
HRGP colocalized with TSP-1 in the stroma of human breast cancer specimens, and this interaction masked the antiangiogenic epitope of TSP-1. Paper-8689729.
In contrast, VEGF- induced proliferation was not affected by HRGP or HRGP330, demonstrating the central role of cell migration during tube formation. Paper-12078379.
Indeed, both HRG and PF4, at physiological concentrations, were shown to effectively inhibit the binding of 125I- aFGF and 125I- bFGF to ECM. Paper-139834.
These data indicate that sustained activation of the MEK/ ERK pathway is both essential and sufficient for HRG-induced differentiation of AU565 cells. Paper-1577501.
The additional complement of zinc in hyperzincemic plasma was localized within fractions containing zinc-binding proteins such as albumin, transferrin and HRG. Paper-4220541.
Presence in human skeletal muscle of an AMP deaminase- associated protein that reacts with an antibody to human plasma histidine-proline-rich glycoprotein. Paper-1718190.
Binding of anti-CD14 mAb MY4 to human monocytes was almost completely abolished by 0.3 microM HRGP and KGP treatments for 15 min, and 1 microM RGP2 for 30 min. Paper-8401526.
The mean (+/- SD) pretreatment concentration of histidine-rich glycoprotein was 1.08 +/- 0.24 U/ml, which was almost identical with the normal reference values. Paper-6906191.
Substantial sequence similarity was identified between the HC hinge region of hIgA1 and regions of maize extensin-family of hydroxyproline-rich glycoproteins ( HRGP). Paper-11464218.
In moderate (Child B) cirrhosis, both HRG levels and total plasminogen levels were reduced, resulting in a normal amount of free plasminogen in circulation. Paper-6307177.
These data suggest that heparan sulfate is the predominate cell-surface ligand for HRG and that mammalian heparanase is a potential regulator of HRG binding. Paper-10346184.
In addition, VEGF- mediated endothelial cell tubular morphogenesis in a three-dimensional collagen matrix was inhibited by HRGP and HRGP330. Paper-12078379.
In contrast, the amount of transforming growth factor beta2 transcript increased only in the HRG (330%; p < 0.01), whereas it remained unchanged in the LRG (-80%). Paper-11443917.
The expression of c-Jun and c-Fos and the level of the phosphorylated c-Jun were markedly increased after HRG-beta treatment in MCF-7/ HER2 cells. Paper-13051552.
In the present paper we report that Rac1 is an essential player for mediating the induction of cyclin D1 and p21(Cip1) by HRG in breast cancer cells. Paper-12704493.
The activation of Glu-plasminogen in alpha 2-AP-depleted plasma containing a normal concentration of HRGP produced a time-dependent increase in the generation of plasmin. Paper-7453301.
Plg activators did not influence binding of Plg to histidine-rich glycoprotein or of histidine-rich glycoprotein to TSP, demonstrating specificity. Paper-5411521.
Our data suggest that HRG-beta- induced MMP-7 expression was regulated by HER2- mediated AP-1 activation in MCF-7 cells. Paper-13051552.
Fibrinogen, which has been previously shown to bind to absorbed thrombospondin, did not inhibit the formation of the thrombospondin- histidine-rich glycoprotein complex. Paper-4759299.
Binding of inhibitors such as alpha2-antiplasmin, plasminogen activator inhibitor-2, lipoprotein(a), or histidine-rich glycoprotein, impairs plasminogen activation. Paper-11275960.
Thrombospondin and histidine-rich glycoprotein are two proteins with diverse biological activities which have been associated with human platelets and other cell systems. Paper-4759299.
In both cases, there were no significant modifications of three proteins with high heparin affinity (antithrombin III, heparin cofactor II, histidine-rich glycoprotein). Paper-5734470.
These studies suggest that HRGP can modulate the antiangiogenic activity of TSP-1, and identify a potential mechanism of resistance to the antiangiogenic effect of TSP-1. Paper-8689729.
Hypothetically, the tandem repetitions of a consensus histidine-rich penta-peptide sequence in HRG may provide a highly charged area that interacts with complement components. Paper-60940.
The results of the immunological analysis reported here demonstrate that an antibody against human plasma HPRG reacts with an AMPD preparation from human skeletal muscle. Paper-1718190.
Histidine-rich glycoprotein complexed with thrombospondin was capable of binding heparin and neutralizing the anticoagulant activity of heparin in plasma. Paper-4759299.
HRG ligands include Zn(2+) and haem, tropomyosin, heparin and heparan sulphate, plasminogen, plasmin, fibrinogen, thrombospondin, IgG, FcgammaR and complement. Paper-10801319.
Interestingly, HRG interfered with the ability of D10 cells to adhere to tissue culture plastic, as well as to laminin-, collagen- or fibronectin-coated culture dishes. Paper-602733.
We have shown previously that HRGP binds with high affinity to thrombospondin-1 ( TSP-1), a homotrimeric glycoprotein that is a potent inhibitor of angiogenesis. Paper-8689729.
Our results indicate that Ava II and Hinc II polymorphisms at the LDLR locus contribute to the variability of total cholesterol and LDL-C levels in HRG individuals. Paper-2090477.
The 5 years event-free survival rate for all patients was 73% (s.e. 2%); for SRG, MRG and HRG patients 85% (s.e. 3%), 73% (s.e. 3%) and 39% (s.e. 7%), respectively. Paper-9473794.
The stoichiometry and dissociation constant (KD) of the complex were determined from the equilibrium distribution of fluorescein-isothiocyanate-labeled PLG in the presence of HPRG. Paper-172840.
Subsequent treatment of GAG-positive Chinese hamster ovary cells with mammalian heparanase or bacterial heparinase III, but not chondroitinase ABC, abolished HRG binding. Paper-10346184.
These observations demonstrate that HPRG can act as either a positive or negative effector of Plg activation in vitro and may serve as a modulator of fibrinolysis in vivo. Paper-10911023.
By removing N-linked sugars with N-glycanase, it could be demonstrated that the difference between the two forms of HRG is caused by an extra carbohydrate group at Asn 184 in form 1. Paper-675806.
However, interactions of TSPs with growth factors, proteases, histidine-rich glycoprotein, and other cell-surface receptors on EC have the potential to modulate CD36-mediated effects. Paper-10020181.
At least two binding sites for HPRG, tropomyosin and heparan sulfate proteoglycans (HSPs), were identified on the surface of FGF-2-activated endothelial cells. Paper-10533417.
At their physiological concentrations, the presence of alpha 2-plasmin inhibitor more markedly decreased the binding of plasminogen to fibrin than histidine-rich glycoprotein. Paper-4590954.
To study the reversible complex formation between the plasma protein histidine-rich glycoprotein ( HRG) and plasminogen, crossed immunoelectrophoresis of HRG was modified. Paper-6131183.
Although the biological function of histidine-rich glycoprotein ( HRG) is unknown, it may serve as an antifibrinolytic agent by interfering with the binding of plasminogen to fibrin. Paper-4970642.
A plasminogen binding protein ( histidine-rich glycoprotein), plasmin inhibitors and activator inhibitors appear to contribute to the regulation of the initial phase of fibrinolysis. Paper-6580946.
We studied the levels of both HRG and plasminogen in patients with different degrees of liver cirrhosis to assess the role of HRG in enhanced fibrinolysis in these patients. Paper-6307177.
HRGP/HRGP330 treatment of endothelial cells induced disruption of actin stress fibers, a process reversed by treatment of cells with the FAK inhibitor geldanamycin. Paper-12078379.
In contrast, the addition of 1.5 microM- HRGP to HRGP-depleted plasma containing a normal amount of alpha 2-AP produced only a modest (17%) decrease in the amount of plasmin(ogen) bound. Paper-7453301.
The purpose of this study was to assay the plasma levels of tPA, PAI, histidine-rich glycoprotein ( HRG), and other fibrinolytic proteins in 15 severely stressed newborns. Paper-7491617.
Addition of PLG shifts the S-value of 125I- labeled HPRG from 4.8S to 6.8S, providing the first direct evidence that HPRG associates with the zymogen form of plasmin in solution. Paper-172840.
Using an enzyme-linked immunosorbent assay, we have demonstrated that purified human platelet thrombospondin formed a complex with purified human plasma histidine-rich glycoprotein. Paper-4759299.
Unlike other stimulators of plasminogen activation, the effect of HPRG on fibrinolysis is modulated by factors that influence the equilibrium between solution and surface-bound HPRG. Paper-946808.
No differences were observed in prekallikrein, high-molecular-weight kininogen, alpha 2-antiplasmin, alpha 2-macroglobulin, antithrombin III, and histidine-rich glycoprotein plasma levels. Paper-4603222.
Structurally, HRG is a modular protein consisting of an N-terminal cystatin-like domain (N1N2), a central histidine-rich region (HRR) flanked by proline-rich sequences, and a C-terminal domain. Paper-10346184.
Each of cystatin-like domains I and II of HRG is encoded by three exons, exons I to III and exons IV to VI, respectively, like those of other members of the cystatin superfamily. Paper-1876309.
Interestingly, the proliferative effect of HRG was impaired not only when the expression of Rac1 or cyclin D1 was inhibited, but also when cells were depleted of p21(Cip1) using RNAi. Paper-12704493.
Histidine-proline-rich glycoprotein ( HPRG) has long been known to associate with plasminogen ( Plg) in solution, but the consequences of this interaction have not been defined. Paper-10911023.
Like platelet factor 4, but unlike histidine-rich glycoprotein, S protein/ vitronectin readily neutralized the anticoagulant activities of heparan sulfate of Mr approximately 20,000. Paper-5738260.
Plasma levels of plasminogen and histidine-rich glycoprotein ( HRG) increased and decreased, respectively, whereas an increase in von Willebrand factor was observed in the treatment group. Paper-137987.
In assays performed in vitro of endothelial cell migration and tube formation, and in vivo corneal angiogenesis assays, HRGP inhibited the antiangiogenic effect of TSP-1. Paper-8689729.
Thus, the peptide (GHHPH)4, derived from the histidine-rich region of HRGP and containing such a heparin-binding motif, was antibacterial for E. faecalis in the presence of Zn2+ or at low pH. Paper-12428358.
The method can be used to assess several aspects of reversible complex formation between plasma proteins, as demonstrated for plasminogen binding of HRG and alpha 2-antiplasmin in whole plasma. Paper-6131183.
We have recently identified several plasma proteins having anti-angiogenic properties, including Histidine-Proline-Rich Glycoprotein ( HPRG) and activated high-molecular-weight kininogen (HKa). Paper-10609103.
The process is regulated by physiological inhibitors, of which alpha 2-antiplasmin, histidine-rich glycoprotein and plasminogen activator inhibitor are reported to be of major physiological significance. Paper-6885500.
Extensive similarity among the NH2-terminal sequences of alpha 2-HS glycoprotein and human histidine-rich glycoprotein suggest that the latter protein is another candidate protein of this new family. Paper-6390164.
Modulation of protein C inhibitor activity by histidine-rich glycoprotein and platelet factor 4: role of zinc and calcium ions in the heparin-neutralizing ability of histidine-rich glycoprotein. Paper-7523895.
These results indicate that HPRG has independent binding sites for heparin and PLG and confirm that one or more lysine residues of HPRG are involved in its recognition by PLG. Paper-172840.
Affinity chromatography of human plasma on Sepharose coupled with the protein specifically absorbed two plasma proteins which were identified as clusterin and histidine-rich glycoprotein ( HRG). Paper-473049.
Thus, HRG, PF4, aFGF, and bFGF all interact with the same HS chains on the 3T3 cell surface, either binding to the same or binding to adjacent saccharide sequences on the chains. Paper-139834.
Histidine-rich glycoprotein ( HRG) reduces by complex formation with plasminogen the amount of "free" plasminogen in circulation and is therefore considered an inhibitor of fibrinolysis. Paper-6307177.
Simultaneous determinations of HRG and total plasminogen combined with a calculation of free plasminogen might yield valuable information when evaluating patients for the availability of plasminogen. Paper-4933441.
The results show a previously undisclosed antibacterial activity of HRGP and suggest that the histidine-rich and heparin- binding domain of HRGP mediates the antibacterial activity of the protein. Paper-12428358.
The interaction of the conserved C-terminal lysine of HPRG with the high affinity lysine binding site of plasminogen is necessary and sufficient to accelerate plasminogen activation. Paper-946808.
Purified HRG or HRG in sera increased the number of HMDM-containing apoptotic cells and accelerated the ingestion, while neutralization or depletion of HRG from sera reduced this effect. Paper-9579646.
However, some divergence is observed between the sequence of one of the fragments liberated from AMP deaminase by a more extensive trypsinization and rabbit plasma HPRG in the region containing residues 472-477. Paper-1171373.
We conclude that TSP1 contains a high-affinity binding site for polyhistidine and this is likely to be the molecular basis for the observed binding of TSP1 to histidine-rich glycoprotein. Paper-8571596.
High levels of histidine-rich glycoprotein ( HRGP) and plasminogen activator inhibitor-1 ( PAI-1) have been claimed to contribute to the hypofibrinolytic state observed in patients with venous thrombosis. Paper-7862153.
Because of the reduction of free plasminogen levels in severe liver cirrhosis, we propose that the decrease in HRG levels in liver cirrhosis plays no role in enhanced fibrinolysis in these patients. Paper-6307177.
On the basis of these findings, it is proposed that HRG and PF4 may act as positive regulators of FGF activity by displacing FGF from the ECM or basement membrane and making FGF available to responsive cells. Paper-139834.
TNF-alpha production by monocytes after HRGP and KGP treatments was decreased at 1 ng/ml, but not at 20 microg/ml LPS, indicating that gingipains inhibited a CD14-dependent cell activation. Paper-8401526.
Inhibition of thrombin by heparin cofactor II and heparin was completely prevented by purified histidine-rich glycoprotein at the ratio of 13 micrograms histidine-rich glycoprotein/microgram heparin. Paper-4988137.
The fourfold-greater increase in aerobic fitness and markedly differing gene expression profile in the HRG indicates that these ECM genes may be critical for physiological adaptation to exercise in humans. Paper-11443917.
The purpose of this study was to measure the plasma levels of HRG and plasminogen in three groups of patients: normal adults (n = 48), normal term newborns (n = 43), and normal premature newborns (n = 18). Paper-6686994.
In contrast, native HPRG bound to hydrazide or nickel chelate surfaces strongly stimulated the activation of plasminogen by tissue plasminogen activator, but not by urokinase or streptokinase. Paper-946808.
Thus, under conditions of local acidosis (e.g. ischemia or hypoxia), HPRG can co-immobilize plasminogen at the cell surface as well as compete for heparin with other proteins such as antithrombin. Paper-1360803.
The sample consists of the adenosine monophosphate deaminase ( AMPD) histidine proline rich glycoprotein ( HPRG) complex that contains 3-4 Zn(II) ions per dimer of approximately 320 kDa molecular weight. Paper-9822831.
Specifically, Plg complexed with HPRG on a GAG surface is more readily activated by tissue-type Plg activator than free Plg, with a 10-fold difference in apparent catalytic efficiency (kcat/Km). Paper-10911023.
The plasma concentrations of histidine-rich glycoprotein and plasminogen were measured and those of free plasminogen calculated in 34 patients with acute myocardial infarction treated with thrombolytic drugs. Paper-6906191.
Complex formation was detected by a specific binding enzyme-linked immunosorbent assay ( ELISA) which demonstrated simultaneous binding of fluid-phase Plg and HRGP to TSP adsorbed to microtitration wells. Paper-5079045.
On the significance of antithrombin-III, alpha 2-macroglobulin, alpha 2-antiplasmin, histidine-rich glycoprotein, and protein C in patients with acute myocardial infarction and deep vein thrombosis. Paper-4855883.
RESULTS: neuropilin-1 (800%; p < 0.001) and VEGF receptor 2 (300%; p < 0.01) transcript abundance increased only in the HRG, whereas levels of VEGF receptor 1 mRNA actually declined in the LRG (p < 0.05). Paper-11443917.
A selective binding of the anti- HPRG antibody to Type IIB muscle fibers was detected, suggesting a preferential association of the novel protein to the AMPD isoenzyme contained in the fast-twitch glycolytic fibers. Paper-1718190.
Partial purification of the activating factor present in RMT-conditioned medium yielded a heparin-binding growth factor with biochemical properties similar to those of neu differentiation factor/heregulin ( NDF/ HRG). Paper-514890.
Translocation of tropomyosin to the surface of HUVEC occurred in response to FGF-2, and the anti-angiogenic activity of HPRG in a Matrigel plug model was partially inhibited by soluble tropomyosin. Paper-10533417.
We tested this hypothesis by comparing the effects of histidine-rich glycoprotein and 6-aminohexanoic acid in an in vitro assay of fibrin-dependent plasmin production mediated by tissue plasminogen activator. Paper-8472816.
Histidine-rich glycoprotein ( HRG) has been reported to be a fibrinolysis regulating protein due to its capacity to bind to the high affinity lysine binding sites of plasminogen. Paper-4933441.
Isolation and characterization of a human plasma protein with affinity for the lysine binding sites in plasminogen. Role in the regulation of fibrinolysis and identification as histidine-rich glycoprotein. Paper-3673509.
Levels of both HRG and plasminogen were higher in the stressed group but the ratio of HRG to plasminogen was the same as that in the normal control newborns (1:3), suggesting an insignificant effect of HRG. Paper-7491617.
The ratio of bound antithrombin, histidine-rich glycoprotein and vitronectin to GAG was negatively correlated with the Orgaran concentration in plasma, implying that the efficacy of Orgaran may not be linearly related to dose. Paper-8228496.
In this study, we show that heregulin-beta ( HRG-beta) stimulation remarkably induced MMP-7 promoter activity and significantly enhanced the expression and activity of MMP-7 in MCF-7 cells overexpressing HER2. Paper-13051552.
S protein/ vitronectin efficiently neutralized oligosaccharides of Mr 2,400-7,200, unlike the two other physiologically occurring heparin neutralizing proteins histidine-rich glycoprotein and platelet factor 4. Paper-5738260.
Furthermore, HRGP inhibited interleukin 2 receptor expression on activated T cells, causing decreased T cell interferon-gamma release and altered T cell-dependent inhibition of erythropoiesis. Paper-6450931.
The results suggest that zinc markedly potentiates the binding of HRG to T cells, and that HRG and zinc may play an important role in regulating the adhesion of T cells to other cells and the extracellular matrix. Paper-602733.
These results provide direct evidence that HRG belongs to a supergene family that includes cystatin SN, SA and C, and kininogen, and also demonstrate high conservation of the intron-exon organization among this supergene family. Paper-5801313.
We have shown that a fragment released from the central histidine/ proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. Paper-12078379.
This structural difference correlates with functional data which suggest that the N-terminal region of the HRG-alpha EGF-like domain is responsible for the observed receptor specificity differences between HRG-alpha and EGF. Paper-555501.
However, neu differentiation factor/heregulin ( NDF/ HRG) mRNA is expressed by the cells, and Western blot analysis of SUM-52PE membrane protein revealed the presence of a M(r) 90,000 immunoreactive NDF/ HRG protein. Paper-548061.
All three parametres showed wide individual variations, but with decreasing functional capacity of the liver the individual levels of plasminogen were reduced earlier than those of HRG leading to decreased amounts of free plasminogen. Paper-4933441.
In addition, we found that HRG activates NF-kappaB (nuclear factor kappaB) in a Rac1- and MEK-dependent fashion, and inhibition of NF-kappaB abrogates cyclin D1/p21(Cip1) induction and proliferation by HRG.