The most recent information on CD46 is here. Click here for the function of CD46. Edit this page in Wiki Genes - CD46 or see Wiki Gene. No spermatozoa were CD46-negative/ vitronectin-positive. Paper-8528316. 2. MCP injection caused no significant changes in plasma ANP. Paper-6921504. Users of Norplant exhibited expression of CD46, CD66 and syndecan-1. Paper-8733819. Hematopoietic progenitors also carried CD46 at intermediate levels. Paper-11992622. Both 5HT antagonists abolished the prolactin release to MCPP. Paper-6631712. MCPP did not significantly alter AVP levels as compared to placebo. Paper-7309106. CD46 also enhanced IL-2 or IFN-gamma secretion by CD4+ blasts. Paper-10504046. The doublet pattern and mol wts are similar to membrane cofactor protein (or CD46). Paper-6832488. MCP-1- Induced Migration of NT2 Neuroprogenitor Cells Involving APP Signaling. Paper-13513372. The role of the complement inhibitors CD46, CD55, and CD59 was also investigated. Paper-9097416. The order of the expression was CR1 greater than DAF = MCP greater than CR2. Paper-52177. Chromatin immunoprecipitation confirms binding of STAT3 to the CD46 promoter. Paper-13387598. IFN-gamma downregulated expression of CD46 on one cell line (p < 0.01). Paper-1267275. MCP-mutated patients had a better prognosis than CFH-mutated and nonmutated patients. Paper-12080071. The epithelium of copper T IUD users expressed CD66 but not syndecan-1 or CD46. Paper-8733819. The dips in the MCPP profile occurred at MC2 and D1, and the peaks at M5, P5, and M4. Paper-1919070. The CD46 receptor was highly expressed in HCC compared to nonmalignant hepatic tissue. Paper-12342495. Fetal neurons were stained for CD59 and CD46 and were negative for CD55 and CD35. Paper-8384404. Thus, our data indicate that both SCR1 and SCR2 make up the MV receptor determinant in CD46. Paper-181966. Our results reveal ADAM10 as an important regulator of CD46 expression during apoptosis. Paper-12071259. Identification of the streptococcal M protein binding site on membrane cofactor protein ( CD46). Paper-9205456. Therefore, we propose that CD46 acts as a potent costimulatory molecule for human T cells. Paper-8387463. Metacarpophalangeal pattern profile ( MCPP) analysis in Williams-Beuren Syndrome ( WBS). Paper-8815878. Using a yeast two-hybrid screen, we identified a physical interaction between CD46 and DLG4. Paper-9155564. The NASA Task Load Index ( TLX) is a popular technique for measuring subjective mental workload. Paper-13595996. Cross-linking CD46 alone induced weak tyrosine phosphorylation of CD3zeta and ZAP-70. Paper-10504046. In summary, the expression of E1A gene products inhibited IFN- MCP independently of virus infection. Paper-7586532. Expression of surface CD20, CD46, CD55, and CD59 was determined by 2-color flow cytometry. Paper-9054967. Active otosclerosis was featured by increased numbers of osteoclasts showing strong CD46 expression. Paper-13219230. 16 patients with rheumatoid arthritis underwent Swanson silastic arthroplasty in 60 MCP-joints. Paper-8136475. Production of transgenic pigs expressing human DAF ( CD55) regulated by the porcine MCP gene promoter. Paper-10588212. A functional interaction between CD46 and DLG4: a role for DLG4 in epithelial polarization. Paper-9155564. Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype. Paper-9742394. TGF-beta present in the supernatant was likely responsible for up-regulation of MCP and CD59. Paper-1720636. CD3/ CD46-activated, but not CD3/ CD28-activated, T cells up-regulate the integrin alpha(4)beta(7). Paper-12900951. HRF20 was most strongly expressed in the peritubular capillaries where MCP was not detectable. Paper-8227321. This situation resembles chromosomal translocations activating genes of the TLX/ HOX11 family in T-ALLs. Paper-12183742. In particular, the BP amplitude was reduced in all areas; the MCP and P200 were also abnormally reduced. Paper-4883214. In conclusion, human NT2-N neurons constitutively express C3aR, CD46, CD55 and, in particular, CD59. Paper-12432747. We show that an interaction between CD46 and DLG4 is important for polarization in epithelial cells. Paper-9155564. MCP produced a marked increase in PAC without a significant change in plasma renin activity. Paper-6921504. Group C, adenovirus serotype 5 (Ad5) infection inhibits both IFN- MCP and cellular protein synthesis. Paper-7586532. Levels of IL-6 and monocyte chemotactic activity (a JE/ MCP activity) correlated with mRNA expression. Paper-7198952. The epithelium of controls expressed CD66 and syndecan-1; but CD46 was found in only one out of six cases. Paper-8733819. MCP- induced PRL release was significantly inhibited up to day 28, with a nadir on day 4. Paper-5570781. Membrane cofactor protein ( CD46) is a keratinocyte receptor for the M protein of the group A streptococcus. Paper-196450. Units of duplication range from single SCRs, to septamers such as efbkdaj, to extensive segments such as MCP- CR1L. Paper-10644665. CD46 possesses four SCR domains and the aim of this study was to characterize their interaction with M protein. Paper-9205456. The glomerular expressions of ICAM1 and MCP were significantly correlated with each other (r = 0.700, P < 0.00001). Paper-618589. MCP mutation- associated HUS has a relapsing course, but none of the children reached ESRD at 1 yr. Paper-13355826. In contrast, fetal astrocytes were strongly stained for CD59, CD46, CD55, and were negative for CD35. Paper-8384404. Contribution of DAF in protecting melanocytes against complement attack was much more than that of MCP. Paper-1389598. Blocking the function of CD59 but not of MCP using monoclonal antibody increased the susceptibility to HuC. Paper-8429402. The effect of CD46 on IL-10 secretion did not require additional costimuli like anti-CD28 antibodies or phorbol esters. Paper-10504046. It is concluded that the B1 and B2 species of Ad engage CD46 through similar binding surfaces. Paper-12526983. These were shown by northern blot and polymerase chain reaction to arise by alternative splicing of CD46 RNA. Paper-7122292. Arthroscopic scores were significantly higher for OC injured carpal than OC injured MCP/ MTP joints (P=0.002). Paper-13538235. PIP joints, MCP joints and wrists of 80 patients with rheumatoid arthritis were operated on with late synovectomy. Paper-2512096. These findings indicate that murine moesin is neither a receptor nor a CD46 coreceptor for MV entry into mouse ES cells. Paper-1307074. Human cells express C-regulatory proteins, CD46 and CD55, thereby circumventing attack by C3, a major effector of C. Paper-1230833. The pure R-enantiomers, both for MCPP and DCPP, after incubation in soil, are partially converted into their S-forms. Paper-8728346. Chondrocytes stimulated with IL-1beta and TNF-alpha showed augmented levels of CD46, CD55 and CD59 mRNA. Paper-9736619. This demonstrates major differences of DC activation upon CD46 activation, with a potential role in the pathogenesis of MS. Paper-12789036. Membrane cofactor protein ( MCP; CD46). Isoforms differ in protection against the classical pathway of complement. Paper-581351. RESULTS: Five hundred forty-four manic patients were psychotic, 364 (33.4%) being MCP, 180 (16.5%) MIP. Paper-12345333. All CD-VLPs formed syncytia after infection in CHO cells expressing CD150 but not in those expressing CD46. Paper-10338600. C3 deposition and HUVEC-surface expression of CD46 and CD55 were evaluated by ELISA and flow cytometry. Paper-1098737. The specificity of the two MB for the corresponding CRP was assessed by ELISA using purified CD46, CD55 and CD59. Paper-10774366. CD46 expression in human HCC and primary hepatocytes was assessed by flow cytometry and immunohistochemistry. Paper-12342495. CONCLUSIONS: While expression of DAF and CD59 on PEC changed its PERV responsiveness to human sera, MCP did not improve it. Paper-11584558. We have generated a mean WS metacarpophalangeal pattern profile ( MCPP) on 21 clinically diagnosed individuals with WS. Paper-7737132. The tetraones of the three and four-layered MCPs (29 and 26) do not show CT interactions except for the six-layered MCP 27. Paper-13175748. However, carpal and MCP/ MTP SF C2C concentrations were not significantly different between rested and exercised groups. Paper-13538235. Suppression of MLR was found only when TLX specificity in sera corresponded to the TLX specificity of the effector cell. Paper-288916. These results prompted us to investigate whether CD46/CD3 costimulation induced the activation of GTPases from the Rho family. Paper-9098859. Dopaminergic and alpha-adrenergic blockade with MCP and THYM respectively, did not have any effect on the GH responses to GRF. Paper-5438782. 3. The increase in PAC induced by MCP injection was markedly attenuated when preceded by the infusion of ANP (25 ng/kg per min). Paper-6921504. Fifty-seven of 260 (22%) were CD46-positive/ vitronectin-positive and 72 of 260 (28%) were CD46-positive/ vitronectin-negative. Paper-8528316. Intracranial administration of MV- CEA into the caudate nucleus of Ifnar(ko) CD46 Ge did not result in clinical neurotoxicity. Paper-9703315. Antibody cross-reactivity with CD46 and lack of cell surface expression suggest that moesin might not mediate measles virus binding. Paper-861888. The most commonly affected joints were ancles (n = 7), MCP joints (n = 6), shoulders (n = 6), MTP joints (n = 6) and wrists (n = 5). Paper-13175063. Cofactor activity of orangutan E was partially inhibited by preclearance of CR1 and more completely inhibited by preclearance of MCP. Paper-6832488. Further, monoclonal antibodies (mAbs) against SCR1 or SCR2 of CD46 blocked MV infection, whereas a mAb against SCR3 and SCR4 did not. Paper-181966. Our data demonstrate that DC-SIGN is crucial as an attachment receptor to enhance CD46/ CD150-mediated infection of DCs in cis. Paper-11311721. For time-resolved spectroscopy a setup consisting of a Czerny Turner spectrometer and a MCP-gated and -intensified CCD camera was used. Paper-9843669. CONCLUSIONS: MCP and MIP manias occurred in nearly half of this largest sample of manic patients ever reported. Paper-12345333. Using these reagents we demonstrate that, like human MCP and DAF, the tissue distribution of Crry/ p65 is very broad. Paper-7578467. In cases when ANOVA showed a significant effect of MCP supplementation, the response of ducks was described by nonlinear functions. Paper-9842830. All the participants were genotyped for the MCP-1-2518G, CCR-2 64I, CCR-5Delta32 and stromal derived factor 1 ( SDF1) 3'A mutated alleles. Paper-12173501. Membrane cofactor protein ( MCP; CD46) is a type 1 membrane glycoprotein that inhibits complement activation on host cells. Paper-1588216. Antibodies to CD46 co-immunoprecipitated an approximately 110-kDa protein band specifically associated with HHV-6-infected cells. Paper-9777478. The order of the genes within an approximately 800-kb DNA fragment on the long arm of chromosome 1 is MCP- CR1- CR2- DAF- C4bp. Paper-6161596. Taken together, the results suggest that the measles virus binding and cofactor activity of CD46 map to distinct areas on the SCR2 module. Paper-8551607. Elevated serum levels of soluble membrane cofactor protein ( CD46, MCP) in patients with systemic lupus erythematosus ( SLE). Paper-1873190. We undertook genetic analysis on membrane cofactor protein ( MCP), complement factor H ( CFH), and factor I (IF) in 156 patients with non-Stx-HUS. Paper-12080071. Therefore, virus binding to the cellular receptor CD46 and subsequent membrane fusion were necessary, but not sufficient, to induce RANTES. Paper-1816449. There was no significant difference between the MCP and MTP joints with respect to RA-based changes obtained in the MRI (p>0.05). Paper-13344711. None of the MCP promoter constructs showed significant increase in CAT expression by treatment with tumor necrosis factor-alpha ( TNF-alpha). Paper-182163. In contrast to E1A-negative, parental cell lines, IFN- MCP was blocked in Ad5 E1A-transfected epithelial and fibroblastic cell lines. Paper-7586532. We showed that CD46 aggregation on human T cells induces p120CBL and linker for activation of T cells ( LAT) phosphorylation. Paper-8387463. Cleavage of MCP was induced close to or within the membrane as demonstrated by the cleavage of transmembrane chimeras of CD59 and MCP. Paper-9326785. Here, the CD46 binding determinants are mapped for the species B1 Ad serotypes 3 and 7 and for the species B2 Ad11. Paper-12526983. CD46 promoter activity is induced by activation of STAT3 and blocked by a dominant-negative form of STAT3 in luciferase reporter assays. Paper-13387598. Our results show that Ad35 competes with measles virus for binding to CD46 but not with complement protein C3b. Paper-10793492. Increased IL-23 secretion and altered chemokine production by dendritic cells upon CD46 activation in patients with multiple sclerosis. Paper-12789036. Ifnar(ko) CD46 Ge transgenic mice, susceptible to MV infection, were used to assess central nervous system toxicity of MV- CEA. Paper-9703315. The findings from this study suggest that both MCPP- induced prolactin release and cortisol release are indeed 5HT-mediated effects. Paper-6631712. In humans, decay-accelerating factor ( DAF) and membrane cofactor protein ( MCP) play such a biologic role by inhibiting C3 and C5 convertases. Paper-146059. Of the other complement regulatory proteins expressed by PMN, only CD59 colocalized with CR1, while CD55 and CD46 were almost absent. Paper-2024331. Several lines of evidence point to the role of c-Yes, a member of the Src family of nonreceptor tyrosine kinases, in CD46 phosphorylation. Paper-9158171. Co-purification of soluble membrane cofactor protein ( CD46) and human herpesvirus 6 variant A genome in serum from multiple sclerosis patients. Paper-10777310. CD46- induced immunomodulatory CD4+ T cells express the adhesion molecule and chemokine receptor pattern of intestinal T cells. Paper-12900951. OBJECTIVE: To examine single nucleotide polymorphisms in both CFH and MCP genes in two large cohorts of HUS patients (Newcastle and Paris). Paper-11456596. Thus, MCP appears to be developmentally regulated in the human liver and is expressed in the absence of DAF on the early hepatic epithelium. Paper-7626443. Anti-CD46 monoclonal antibody (M75 and M160) study made clear that these two substitutions also governed the MV H protein's interaction with CD46 receptor. Paper-9210764. Quantitative scintigraphy of the wrist, MCP, and knee joints in patients with RA was performed using Tc-99m-MDP and Ga-67 citrate. Paper-5677378. We have also compared the reliabilities of P. stuartii identification by commercial kits ( API 20E system) by using a standard MacConkey or MCP medium. Paper-7526046. These findings suggest a role for 5-HT1c/ 5-HT2 receptors in the endocrine and behavioral responses to the mixed serotonin agonist MCPP in humans. Paper-7029952. RESULTS: The mean neck-extension angles were MCP - 2.6 degrees, LS 9.5 degrees, LR 8.1 degrees, ES 23.9 degrees and ER 25.4 degrees ( ANOVA p < 0.0001). Paper-10219093. In this study, we determined the adhesion protein and chemokine receptor expression pattern on human CD3/ CD46-activated peripheral blood CD4(+) T cells. Paper-12900951. 3. Compared with the sham-operated group, contractions in the clot removal groups at 48 and 72 h after SAH were reduced in MCP and enhanced in BAP. Paper-1933081. The CD46 promoter contains two binding sites for activated STAT3 and mutations introduced into the major site abolished STAT3 binding. Paper-13387598. CD3/ CD46- activated Tregs adhere to/roll on MAdCAM-1-expressing HeLa cells, similar to T cells isolated from the human lamina propria (LP). Paper-12900951. Expression of CD59, a complement regulator protein and a second ligand of the CD2 molecule, and CD46 in normal and neoplastic colorectal epithelium. Paper-7601166. Indeed, we report that the small GTPase Rac is also activated upon CD46/CD3 costimulation, whereas no change of Rho and Cdc42 activity could be detected. Paper-9098859. We also propose that PTPRC and REN genes map to a region of the RCA gene cluster between the HF1/ F13B and C4BP/ MCP regions. Paper-558533. Nevertheless, neither substances inhibiting capping nor the fusion-inhibiting peptide Z-D-Phe-L-Phe-Gly-OH ( FIP) blocked CD46 downregulation. Paper-1910539. RESULTS: Complement regulatory proteins such as C1-INH, CD55, CD46, and CD59 were found in the sperm extracts as shown by Western blot analysis. Paper-1422465. We also tested whether the cocaine addicts differed from healthy volunteers on their PRL, cortisol ( CORT) or temperature responses to MCPP. Paper-1597635. IFN- MCP was not inhibited by infection with dl343, despite the production of large amounts of both early (E1B, p55) and late (hexon) Ad proteins. Paper-7586532. DAF up-regulation was first detectable at 6 hours and maximal 24 hours poststimulation, whereas no up-regulation of CD59 or MCP was seen. Paper-8533597. The aim of this study was to investigate a possible correlation between elevated levels of soluble CD46 and the presence of serum HHV-6 DNA in MS patients. Paper-10777310. Changes in the nephrologist MCP this year and the MMA changes to provider reimbursement coming in 2005 are reprioritizing the parameters for reimbursement. Paper-10865098. The complement regulators CD55 and CD59 were found primarily on the inner retina, while CD46 was present exclusively in a polarized fashion on the RPE. Paper-12206719. In contrast to CD46 and CD150, DC-SIGN does not support MV entry, since DC-SIGN does not confer susceptibility when stably expressed in CHO cells. Paper-11311721. Otosclerosis is a disease of disturbed osteoid turnover due to persistent measles virus infection and special CD46 receptor pattern of the otic capsule. Paper-13219230. The administration of MCP induced a striking elevation in plasma AVP levels in the normal controls and in the diabetic subjects of groups I and II. Paper-407094. Before adenomectomy, metoclopramide ( MCP; 10 mg orally) and TRH (200 micrograms iv) did not increase PRL blood levels in any of the seven patients. Paper-3120248. The results indicate that CD46 is expressed preferentially on the apical membranes while moesin appears to be present at similar levels on both surfaces. Paper-265248. After transfusion and in pregnancy the induction of TLX alloantibody production depended on the mismatching in the TLX/ CD46/ MCP phenotypes. Paper-288916. Previous studies suggested that transfusion induces the production of EAI blocking antibody which may have specificity to TLX/ CD46/ MCP alloantigens. Paper-862881. A monoclonal anti-TGF-beta antibody, which neutralized TGF-beta1, -beta2 and -beta3, did not inhibit the up-regulation of MCP and CD59 by the supernatant. Paper-1720636. The identification of CD46 as a virus receptor and of the involvement of moesin sheds some light on the molecular events occurring during virus entry into the cell. Paper-437609. However, we did not find that porcine endothelial cells expressing both hDAF and hMCP were better protected than those expressing hDAF alone. Paper-9206599. CD46 (membrane cofactor protein, MCP) is a cell surface complement regulatory protein which may have an additional role in human sperm-egg interaction. Paper-850221. We conclude that GC infection leads to rapid tyrosine phosphorylation of the CD46 Cyt2 tail and that the Src kinase c-Yes is involved in this reaction. Paper-9158171. These CD3/ CD46- stimulated IL-10-producing CD4+ cells proliferate strongly, suppress activation of bystander T cells and acquire a memory phenotype. Paper-9742394. The demonstration herein that neurons express only very low levels of CD59 and MCP and lack both CR1 and DAF might explain their susceptibility to C damage. Paper-8304552. Purification and functional properties of soluble forms of membrane cofactor protein ( CD46) of complement: identification of forms increased in cancer patients' sera. Paper-378825. METHODS: Flow cytometric analysis of CD46 antibody versus lectins (PNA, PSA, and Con A) was used to quantify the acrosome reaction of human sperm. Paper-974677. We recently characterized the domains of the human cofactor protein CD46 involved in binding species B2 adenovirus ( Ad) serotype 35. Paper-12526983. In addition, we determined that short consensus repeat 2 ( SCR2), -3, and -4 of the CD46 ectodomain were essential for the formation of the virus-induced syncytia. Paper-9211650. Thus part of the difference in the magnitude of blood flow during the MRP vs. MCP may be due to the alterations of the blood velocity flow profile. Paper-12226735. Indeed, we show in this study that CD46/CD3 costimulation induces a synergistic activation of extracellular signal-related kinase mitogen-activated protein kinase. Paper-9098859. Nevertheless, certain malignant cells, particularly those undergoing apoptotic stress, can activate homologous C, overcoming the regulatory actions of CD46 and/or CD55. Paper-1230833. Syncytiotrophoblast is unique in containing a 110 kDa form of MCP in non-reducing SDS-PAGE that resembles 65 kDa MCP in glycosylation. Paper-7425017. Recent literature has suggested that approximately 50% of patients have mutations in factor H ( CFH), factor I ( CFI), or membrane cofactor protein (encoded by CD46). Paper-13539535. Here, we examined the role of CD46, a complement binding factor, in mDCs by analyzing cytokine and chemokine production in healthy donors and patients with MS. Paper-12789036. Crosslink of the MV-receptor CD46 with antibodies and treatment of cells with purified viral glycoproteins led to the induction of small but significant amounts of IL-6. Paper-1699618. We have proposed that Crry/ p65 has a similar biologic role in mouse as MCP and perhaps DAF and is the mouse analogue of one or both of these proteins. Paper-7578467. Normal human sera contained 10-60 ng/ml of soluble membrane cofactor protein ( MCP, CD46) whereas sera of > 50% of the cancer patients contained > 60 ng/ml. Paper-378825. Here we show that, despite their IL-10 production, CD46-induced T-regulatory T cells (Tregs) do not suppress the activation/maturation of dendritic cells (DCs). Paper-10821429. Our data show a strong binding of CD46 to bacteria of M types 1, 3, 8, 18, 24, 28, 29, 31 and 78; weak binding to M6 and M29 and no binding to M types 11, 12, M27 or M30. Paper-13247929. TLR- mediated functional potential of DCs may affect the degree of MV amplification through distinct MV strain-specific receptor usage of CDw150 or CD46. Paper-9561671. NT2-N neurons expressed significant amounts of CD59 (Clone H19/Clone BRIC229: p=0.000006/p=0.000003), CD46 (p=0.00006), CD55 (p=0.003) and C3aR (p=0.00003). Paper-12432747. Factor I ( FI) is the major complement inhibitor that degrades C3b and C4b in the presence of cofactors such as factor H ( FH) and membrane cofactor protein ( MCP). Paper-12371807. An immunoaffinity column comprised of immobilized monoclonal antibodies to CD46 was developed to isolate sCD46 from cell free body fluids of MS patients and controls. Paper-10777310. Here we show that co-engagement of CD3 and the complement regulator CD46 in the presence of IL-2 induces a Tr1-specific cytokine phenotype in human CD4+ T cells. Paper-9742394. In conclusion, hematopoietic progenitor cells express CD46 on their surface, thereby fulfilling a basic requirement for the susceptibility of HHV-6 infection. Paper-11992622. Based on these results, p60 is a plasma C3b- binding protein that shares epitopes with C4bp and MCP, and is probably not a soluble form of MCP. Paper-6322. GC infection causes c-Yes to aggregate in the host cell cortex beneath adherent bacteria, increases binding of c-Yes to CD46, and stimulates c-Yes kinase activity. Paper-9158171. A moderately increased frequency of MCP-1-2518 A allele (p = 0.033, pC = NS) and AA genotype (p = 0.017, pC = NS) existed in SLE patients compared with healthy controls. Paper-13441788. Measurements were focused on the requested effects and acquired using various techniques including questionnaires, interviews, observations, and Task Load Index ( TLX) ratings. Paper-12318895. Expression of complement inhibitors CD46, CD55, and CD59 on tumor cells does not predict clinical outcome after rituximab treatment in follicular non-Hodgkin lymphoma. Paper-9054967. To analyse the mechanisms involved in these phenomena, P-selectin, ICAM-1, VCAM-1 and MCP- 1 expression were evaluated in HuVEC seeded on the different ECM components. Paper-11993959. A mathematical model, which effectively discriminates WS patients from unaffected control individuals, was developed using discriminant analysis of the MCPP data. Paper-7737132. Mutations in factor H ( CFH), factor I (IF), and membrane cofactor protein ( MCP) genes have been described as risk factors for atypical hemolytic uremic syndrome (aHUS). Paper-13355826. In SLE, HRUS with PD detects a high prevalence of inflammatory pathology in the tendons and synovium of the hand and wrist, and a high prevalence of MCP joint erosions. Paper-12202044. When used in combination with MCPP, alprazolam blunted MCPP- induced cortisol and GH release, and it blocked the anxiogenic effects of MCPP. Paper-8108657. After MCP treatment, serum LH did not change, but serum PRL increased more in PCO (801 +/- 100%) than in normal women (467 +/- 73%; P less than 0.05), as did serum TSH. Paper-5286169. Recipients of hDAF or hDAF/ hMCP hearts did not differ in their patterns of serum antiporcine antibodies or in plasma levels of the soluble terminal complement complex sC5b-9. Paper-10646983. Kidney transplantation outcome was favorable in patients with MCP mutations, whereas the outcome was poor in patients with CFH and IF mutations due to disease recurrence. Paper-12080071. Furthermore, good YIG crystals can be obtained by treating the MCP precursor with a low calcination temperature at 600 degrees C from the XRD spectra and TEM micrograph. Paper-12384772. Using microarray gene expression profiling, we identified the CD46 gene as a target for activated STAT3 signaling in human breast and prostate cancer cells. Paper-13387598. Decreased PRL and aldosterone responses to MCP may reflect decreased ambient dopamine levels and/or a reduction in dopamine receptor number or binding affinity. Paper-3717409. Our results suggest Trop 3 MAb might recognize a monomorphic determinant of TLX antigens and Trop 4 is involved in cell proliferation or in cell-to-cell interaction. Paper-5203258. The ADAM10- mediated release of CD46 from apoptotic vesicles may represent a form of strategy to allow restricted complement activation to deal with modified self. Paper-12071259. Recently, mutations and polymorphisms in complement regulator molecules FH and MCP but also in FI have been associated with atypical hemolytic uremic syndrome (aHUS). Paper-12371807. Unexpectedly, CD46 expression on progenitors from G-CSF mobilised leukapheresis products was approximately 20% of that found on comparable cells from untreated cord blood. Paper-11992622. We present evidence that MCP-1 binding to CCR2 induces a novel transcription factor ( MCP-induced protein [ MCPIP]) that causes cell death. Paper-12002716. Furthermore, a cell line lacking DAF (IA10-/D17-) allowed alternative pathway- mediated homologous complement ( C3) deposition after pretreatment with anti- MCP antibody. Paper-7576932. This protein induced extensive syncytia in cells expressing the normal virus receptor CD46 and also in CD46-negative cells expressing the targeted receptor, human CEA. Paper-8692766. CD46/CD3 costimulation induces morphological changes of human T cells and activation of Vav, Rac, and extracellular signal-regulated kinase mitogen-activated protein kinase. Paper-9098859. The immunohistochemically stained membrane cofactor protein of complement ( MCP/ CD46), one of the complement regulatory proteins, was up-regulated in some diseased kidney tissues. Paper-101415. Assignment of MCP encoding the porcine membrane cofactor protein ( MCP/ CD46) to the long arm of pig chromosome 9 with somatic cell hybrids. Paper-2010542. The H-mediated CD46 downregulation is reversible and restricted to CD46 since expression of other surface markers ( CD3, CD14, CD47 and CD63) is unaffected. Paper-413506. The inhibition of MCP secretion was caused at the level of gene transcription, because MCP-1 mRNA levels in oligonucleotide-treated cells were also decreased by approximately 40%. Paper-2121484. RESULTS: Neither PSA nor Con A was able to detect significant changes in the spontaneous and ionophore- induced acrosome reactions compared to CD46 antibody. Paper-974677. In the human system, an IL-10-secreting, T regulatory type 1 cell (Tr1)-like subset of Tregs can be induced by concurrent cross-linking of the TCR and CD46 on naive CD4(+) T cells. Paper-10768337. METHODS: CD4 lymphocytes were isolated from patients with IPEX-like syndromes and activated with antibodies to CD3 and CD46 to generate IL-10-expressing T regulatory cells. Paper-13104857. After all, human cells undergo almost no autologous complement-mediated cytolysis if they express at least one of the functionally active inhibitors, MCP, DAF, or p18. Paper-22837. Of a total of 1,278 seeded urine samples from elderly patients, we isolated 103 P. stuartii strains which were all correctly identified by coupling MCP medium and the API 20E system. Paper-7526046. In addition, activation of CD46 through streptococcal M protein induced the expression of granzyme B, providing a second means for these cells to regulate an immune response. Paper-10768337. Antimoesin antibodies also reacted with CD46/ CD4 molecules containing only the SCR I and II domains required for measles virus (MV) hemagglutinin binding onto CD46. Paper-861888. No significant difference was found between the change in distance of the humeral head and the glenoid fossa after traction in the MLPP compared to traction in the MCPP. Paper-9781972. For examples, Langerhans cells (LCs) migrate selectively in response to CCL20/MIP-3alpha (through CCR6), blood CD11c+ DC to MCP chemokines (through CCR2). Paper-10209256. These results show that at least three members of this family confer membrane cofactor protein-like activity ( MCP or CD46) upon cells expressing RGD-binding integrins or CD44. Paper-9160269. Prior to this maturation stage, Mphi expressed sufficient amounts of CD9 and CD46 but showed no such complex formation, and as in intact monocytes MV replication was markedly suppressed. Paper-9195287. U-937 possessed DAF and MCP but lacked p18, and about 50% was lysed by treatment with anti-DAF and anti-MCP followed by Mg(2+)-EGTA-serum, which caused C3, C5 and C8 deposition. Paper-38328. SCR2 and SCR3 were required for C3b and C4b inactivation, and SCR4-deleted MCP showed weak cofactor activity for C4b cleavage but virtually no cofactor activity for C3b cleavage. Paper-272232. CD46 (membrane cofactor protein; MCP) is a molecule that functions as either a complement-regulatory protein ( CRP) or a receptor for some pathogens, including human herpesvirus 6. Paper-13173665. Thus, the reported MV-mediated NO production, irrespective of whether it is IFN-gamma-dependent or -independent, should be largely attributable to CD46 signaling but not to MV replication. Paper-9597041. We have found using RNA interference analysis that a disintegrin and metalloproteinase ( ADAM) 10 is responsible for the regulated shedding of the ectodomain of CD46 in apoptotic cells. Paper-12071259. Whereas FH mutations were reported in both familial and sporadic forms of HUS, CD46 mutations were restricted to familial HUS, and factor I mutations were only observed in cases of sporadic HUS. Paper-11465603. RESULTS: The prevalence of severe malnutrition was the highest in TAP (39.1%) HFP (25.6%) and MAP (24.3%) and the lowest in SCP (4%), SP (4.8%), DNP (5.7%) and MCP (7.4%) (P < 10(-4)). Paper-10492696. Co-cultivation of mouse macrophages bearing human CD46 with Chinese hamster ovary (CHO) cells expressing MV H and F enhanced IFN-gamma-induced NO production. Paper-9597041. Furthermore, when these cells were transfected to express human CD46, a 100-fold increase in syncytium formation was observed with these cells and was independent of the expression of moesin. Paper-1307074. Altogether these data showed that the specific STP regions of CD46 played no major role in HA-mediated MV binding to CD46, virus infection and virus-induced down-regulation of CD46. Paper-130850. Premedication with ritanserin attenuated the MCPP- induced increases in self-rated anxiety and prolactin, and completely antagonized MCPP cortisol elevations. Paper-7029952. CD46 mRNA expression is induced by interleukin-6 and by transient transfection of normal human epithelial cells with a persistently active mutant construct of STAT3, STAT3C. Paper-13387598. Strikingly, recombinant granulocyte CSF treatment of myeloid cell lines and hemin treatment of an erythroblastoid cell line, K562, led to a decrease of MCP to near normal levels. Paper-6603818. Replication-competent MV with H replaced by H displaying the long-linker form of scAb was recovered and replicated efficiently in both CD46-positive and CD46-negative, CEA-positive cells. Paper-8692766. We present data using our recently developed large frame CCD centroiding detector and have implemented a new high speed MCP high voltage pulser with an effective slice width of only 6 ns. Paper-12076764. We previously demonstrated that the recipient MCP-1-2518G polymorphism is associated with increased CCL2/ MCP-1 production by inflammatory cells and decreased renal allograft survival. Paper-13514403. Human cells express cell surface complement regulatory molecules that inhibit the activity of the C3/ C5 convertases ( DAF, MCP, CR1) or inhibit the membrane attack complex ( CD59). Paper-407677. The presence of excess M6 protein did not inhibit the cofactor activity of CD46 and the presence of excess C3b did not inhibit the ability of CD46 to bind M6 protein by ELISA. Paper-9205456. We suggest that the weak cross-reactivity of antimoesin antibodies with CD46 explains the inhibitory effect of these antibodies on MV entry and that moesin is not directly involved in MV binding. Paper-861888. METHODS: Fluorescent flow cytometry was used to quantitate the binding of monoclonal antibodies (mAb) to sperm-bound CD15 and CD46 antigens on acrosome-intact (AI) and acrosome-reacted (AR) sperm. Paper-1011904. Ten healthy male subjects were administered i.v. meta-chlorophenylpiperazine ( MCPP) (0.1 mg/kg) after oral ritanserin (5-10 mg), a putative 5HT1c/ 5HT2 ( serotonin) antagonist, or placebo. Paper-7029952. Thus, CD46, CD55, and CD59 expression on pretreatment tumor cells, or their susceptibility to in vitro complement-mediated killing, does not predict clinical outcome after rituximab treatment. Paper-9054967. The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein). Paper-12060338. The combination of tumour necrosis factor-alpha, IL-1beta, and IL-6 caused increased expression of CD55 (three-fold) and CD59 (two-fold) and decreased expression of CD46 at day 3 post-exposure. Paper-8372634. Application of Spearman rank correlation test revealed CD46 expression was significantly associated with expression of ER at the level of protein (p = 0.031; r = 0.31) and mRNA (p < 0.001; r = 0.52). Paper-10216153. The high transduction efficiency is in line with high expression levels of CD46 detected on migratory cutaneous DC, which proved to be further increased upon intradermal administration of GM-CSF and IL-4. Paper-12163929. Expression of the complement-regulatory proteins ( CRP) CD46, CD55 and CD59 represents a strategy used by tumor cells to evade complement-dependent cell cytoxicity stimulated by monoclonal antibodies. Paper-10774366. Stimulation with PHA did not significantly alter NK cell surface CReg protein levels whereas, following culture with IL-2, CD46 and CD59 were decreased on both CD56bright and CD56dim subsets (p<0.05). Paper-12557037. SCR1/ SCR2 of Cremp were 43.6% identical with SCR2/ SCR3 of human decay-accelerating factor ( CD55), and SCR3/SCR4 were 45.3% identical with those of human membrane cofactor protein ( CD46). Paper-8688239. The role of CD44, CD45, CD45RO, CD46 and CD55 as potential anti-adhesion molecules involved in the binding of human tonsillar T cells to phorbol 12-myristate 13-acetate-differentiated U-937 cells. Paper-6576621. CD46 activation of T cells also induced the expression of the surface- bound cytokine LIGHT and the chemokine receptor CCR9, both marker constitutively expressed by gut LP-resident T cells. Paper-12900951. In one case, CR2 or its related molecules participates in C3 deposition overcoming the protective function of DAF/ MCP and this type of C3 deposition is maximized under physiological conditions. Paper-7501128. This DAF-TM construct used the TM and cytoplasmic domains of membrane cofactor protein ( MCP); an alternate TM version of DAF constructed with the TM and cytoplasmic domains of HLA-B44 showed equivalent protection. Paper-6928999. The aim of this study was to evaluate the effectiveness of the ergoline derivative cabergoline in inhibiting the serum PRL response to metoclopramide ( MCP; 5 mg, iv) and the duration of this effect. Paper-5570781. They participate through the synthesis and secretion of pro-inflammatory cytokines, including interleukin 1 ( IL-1), IL-6, and IL-8, as well as M-CSF, G-CSF, GM-CSF, gro alpha, and MCP. Paper-7568037. High expression of membrane cofactor protein of complement ( CD46) in human leukaemia cell lines: implication of an alternatively spliced form containing the STA domain in CD46 up-regulation. Paper-463631. After transfusion, TLX alloantibody production with Fc tau R and MLR blocking function was induced only when the recipient was lacking the TLX specificities expressed on the donor cells. Paper-288916. These data suggest that CD46- induced Tregs might play a role in intestinal immune homeostasis where they could dampen unwanted effector T cell responses through local IL-10/ granzyme B production. Paper-12900951. MCP (2-MCP and 4-MCP) were the dominant products and phenol partly remained in acid media, while TCP and DCP ( 2,6-DCP and 2,4-DCP) were the main components in neutral and alkaline media. Paper-12103873. Paired samples of tumor and normal tissue from 22 bladder cancer patients were analyzed for expression of MUC1, CD46, CD55 and CD59, and matched serum samples analyzed for anti-MUC1 IgM and IgG levels. Paper-12939305. DC maturation by complement/ CD46- induced Tregs is mediated through simultaneous secretion of GM-CSF and soluble CD40L, factors favoring DC differentiation and reversing inhibitory effects of IL-10. Paper-10821429. Decay-accelerating factor ( DAF or CD55) and membrane cofactor protein ( MCP or CD46) function intrinsically in the membranes of self cells to prevent activation of autologous complement on their surfaces. Paper-8530397. Chimeric proteins exchanging the four extracellular short consensus repeat (SCR) domains between CD46 and DAF indicated that only molecules with both SCR1 and SCR2 from CD46 allowed a productive MV infection. Paper-181966. The role of the regulatory proteins was examined by pretreating cells with mAb to block decay accelerating factor ( DAF) or membrane cofactor protein ( MCP) or by using factor H depleted sera as a complement source. Paper-190447. We found a significantly reduced expression of CD46 and CD59 on CD4+ alveolar lymphocytes from HIV-1 infected individuals, whereas the expression of CR3, CR4, ICAM-1 and CD30 was increased. Paper-1180308. PURPOSE: To assess the value of the metacarpophalangeal pattern profile ( MCPP) analysis as a diagnostic tool for differentiating between patients with dyschondrosteosis, Turner syndrome, and hypochondroplasia. Paper-12047767. Human complement-mediated cytolysis assays showed that co-expressed DAF and MCP proteins could provide more significant protection against complement- mediated cytolysis than either hMCP or hDAF alone. Paper-13066405. The human complement RCA proteins analyzed were factor H ( FH), C4 binding protein alpha chain, membrane cofactor protein ( MCP), decay accelerating factor (DAF), and complement receptors type 1 ( CR1) and 2 (CR2). Paper-10746585. Because the Edmonston vaccine strain can enter cells through CD46 in addition to the primary MV receptor signaling lymphocyte activation molecule (SLAM or CD150), we asked whether and how its tropism is altered. Paper-13305269. Functional modulation of human macrophages through CD46 ( measles virus receptor): production of IL-12 p40 and nitric oxide in association with recruitment of protein-tyrosine phosphatase SHP-1 to CD46. Paper-8594490. Moesin, and not the murine functional homologue ( Crry/ p65) of human membrane cofactor protein ( CD46), is involved in the entry of measles virus (strain Edmonston) into susceptible murine cell lines. Paper-318000. To compare the leukemogenic properties of HOXA13 to that of TLX proteins, cohorts of lethally irradiated mice were transplanted with bone marrow transduced with a retroviral vector expressing TLX3 or HOXA13. Paper-12183742. To determine if malfunction in the MV receptor CD46 is responsible for failure of IkappaBalpha phosphorylation and degradation, neuronal cells were transfected with a wild type CD46 (CD46-WT) expression vector. Paper-8854722. MCP mutations were detected in affected individuals of three families: a deletion of two amino acids (D237/S238) in family 1 (heterozygous) and a substitution, S206P, in families 2 (heterozygous) and 3 (homozygous). Paper-10165229. Since CD46 was consistently expressed in colorectal carcinomas the low expression or even lack of CD59 in a subset of tumours might not lead to critical complement-mediated attack of CD59-negative tumour cells. Paper-7601166. We have previously shown that the expression of human CD46 in mouse macrophages restricts MV replication in these cells and enhances the production of nitric oxide (NO) in the presence of gamma interferon ( IFN-gamma). Paper-9597041. RESULTS: No significant differences were found in mean distance between the humeral head and the glenoid fossa during traction in the MLPP compared to traction in the MCPP (O1: P=1.00) and ( O2: P=0.63). Paper-9781972. We recently showed that CD46, a complement regulatory receptor for C3b as well as a receptor for several pathogens, could act as a potent costimulatory molecule for human T cells, highly promoting T cell proliferation. Paper-9098859. The outcome of the disease reported here indicates that MCP mutation and complete paternal uniparental disomy of chromosome 1 could have an additive effect in determining the severity of the HUS phenotype. Paper-12444731. However, CD46 cross- linking by itself did not induce cell proliferation or lymphokine secretion, and pretreatment of CD4+ T lymphocytes with anti- CD46 antibodies did not significantly alter TCR/CD3 activation. Paper-10504046. The percentage of PSA-negative/ CD46-positive spermatozoa increased to a greater extent than that of PSA-negative/ CD46-negative spermatozoa with an increase in the incubation time. Paper-1750179. CAB-2, a chimeric protein constructed from genes encoding the complement regulatory proteins CD46 and CD55, inactivates the C3/ C5 convertases and blocks in vitro generation of C3a, C5a, and C5b-9. Paper-1935703. Bidirectional sequencing of CFH, CFI, and CD46 confirmed that the patient was heterozygous for a novel missense mutation, a substitution of a serine reside for a tyrosine residue at amino acid 369, in CFI. Paper-13539535. Presence of measles virus RNA was demonstrated in the footplates of histologically diagnosed otosclerotic patients by RT-PCR; however, no reports were available about the CD46 expression pattern and level in otosclerosis. Paper-13219230. M6 protein did not bind to soluble recombinant CD46 chimeric proteins that had the third and/or fourth SCR domains replaced with the corresponding domains from another complement regulator, CD55 (decay-accelerating factor). Paper-9205456. This review will focus on what is currently known about the genetic regulation of key protective complement receptors genes including CR1 ( CD35), CR2 ( CD21), Crry, MCP ( CD46), DAF ( CD55), and CD59. Paper-12567769. The efficiency with which different measles virus (MV) strains enter cells through the immune cell-specific protein SLAM ( CD150) or other receptors, including the ubiquitous protein CD46, may influence their pathogenicity. Paper-9214980. A similar natural evolution and response to treatment would be expected for the three patients, as they all presented with the same at-risk polymorphisms for CFH and CD46 and no identifiable mutation in either CD46 or CFI. Paper-12881355. These results indicated that TGF-beta and an additional factor(s) present in the supernatant may be responsible for up-regulating the expression of MCP and CD59 on keratinocytes; both may be acting non-synergistically. Paper-1720636. Activation of human primary CD4(+) T cells in the presence of CD46 cross- linking induces the development of a distinct immunomodulatory T cell population characterized by high IL-10/ granzyme B production. Paper-12900951. Ligation of CD46 on human T cells prevented recruitment of the microtubule organizing center, CD3, and perforin to the interface with the antigen-presenting cell and caused a reduction in IFN-gamma production. Paper-12348193. Expression of CD46 on B lymphocytes increased MHC class II-dependent presentation of MV H up to 100-fold, while CD46-transgenic DC stimulated H-specific T cell hybridomas up to 10-fold better than nontransgenic DC. Paper-1402629. A higher blood flow furthermore appeared during the MRP compared to during the MCP, coinciding with a greater uniformity of the red blood cells moving at higher blood velocities during the MRP. Paper-12226735. METHODS: Infarct volume and total intracranial volume were measured with Leica Q500 MCP image analysis software, or with a caliper, on 38 CT scans of patients who participated in the Tirilazad Efficacy Stroke Study II ( TESS II). Paper-8764096. Measles virus (MV) enters cells either through the signaling lymphocyte activation molecule SLAM ( CD150) expressed only in immune cells or through the ubiquitously expressed regulator of complement activation, CD46. Paper-10283297. The expression of CR1, as well as the complement receptor type 2 ( CR2) and membrane cofactor protein ( MCP), were analyzed on cells from the SUP- T1, CEM-SS, HUT-78, and MOLT-3 cell lines by flow cytometry. Paper-240859. Membrane expression of decay-accelerating factor ( DAF), membrane cofactor protein ( MCP), complement receptors (CR)1, CR2 and H was measured either by radioimmunoassay or enzyme-linked immunosorbent assay on cell lysates. Paper-6658212. So far, mutations in single genes coding for the cofactor and complement regulator factor H, the membrane cofactor protein ( MCP/ CD46), the serine protease factor I, and autoantibodies to factor H have been linked to HUS. Paper-11361599. Furthermore, TLX interacts with atrophin1 ( Atn1), a corepressor that is involved in human neurodegenerative dentatorubral-pallidoluysian atrophy ( DRPLA) and that is essential for development of multiple tissues. Paper-12006573. In this study, we used competition experiments with known CD46 ligands, CD46-specific antibodies, and a set of CD46 mutants to localize the binding domain for the group B adenovirus serotype 35 ( Ad35). Paper-10793492. MV-GFP-H(AA)-IL-13 was generated from the Edmonston-NSe vaccine strain, by displaying human IL-13 at the C-terminus of the H protein, and introducing CD46 and signaling lymphocyte activation molecule (SLAM)-ablating mutations in H. Paper-12975555. Moesin-negative ES cells expressing or not expressing human CD46 formed separate pieces of fragmented syncytia which were torn apart during spreading, whereas ES cells expressing moesin exhibited typical syncytia. Paper-1307074. Two months later, four of the men received placebo on day 0, and tests with MCP were performed on days -1, 8, and 28; basal serum PRL levels and PRL responses to MCP were superimposable on days -1, 8, and 28. Paper-5570781. Among vertebrate receptors, PNR shares structural kinship with an orphan receptor TLX, and despite distinct differences in the DNA binding domain, PNR is able to recognize a subset of TLX target sequences in vitro. Paper-1857142. MCP-1-2518, SDF-1 G801A, and RANTES-28 polymorphisms were determined in 242 patients with SLE and 220 ethnically matched healthy controls by the polymerase chain reaction-restriction fragment length polymorphism technique. Paper-13441788. We examined the effects of F(ab')2 of polyclonal antibodies raised against DAF and MCP and monoclonal antibody to p18 on the susceptibility of K562 to cytolysis by homologous lymphocytes, granulocytes and complement. Paper-44149. In transwell assays, iDC migrated towards SDF-1, MIP-1alpha, MCP and S1P, whereby S1P combined with a chemokine had a synergistic effect. mDC migrated towards 6Ckine and MIP-3beta, but not towards S1P. Paper-12004306. PURPOSE: To analyse change in distance between the humeral head and the glenoid fossa during traction in the maximally loose-packed position ( MLPP) and the maximally closed-packed position ( MCPP) under standardized conditions. Paper-9781972. DCs express the MV receptors CD46 and CD150, and we demonstrate that the C-type lectin DC-specific intercellular adhesion molecule 3-grabbing nonintegrin ( DC-SIGN) is a novel receptor for laboratory-adapted and wild-type MV strains. Paper-11311721. Adenovirus-mediated gene transfer of triple human complement regulating proteins ( DAF, MCP and CD59) in the xenogeneic porcine-to-human transplantation model. Part II: xenogeneic perfusion of the porcine liver in vivo. Paper-9162945. Following confirmation of the M6 protein-dependent interaction between GAS and human keratinocytes, we demonstrated that M6 protein binds soluble recombinant CD46 protein and to a CD46 construct containing only SCRs 3 and 4. Paper-9205456. Using genetic and biochemical approaches, we show that TLX modulates retinal progenitor cell proliferation and cell cycle re-entry by directly regulating the expression of Pten and its target cyclin D1. Paper-12006573. Concurrent activation of the T-cell receptor (TCR) and complement regulator CD46 on human CD4+ T lymphocytes induces Tr1-like regulatory T cells that suppress through IL-10 secretion bystander T-cell proliferation. Paper-10821429. Reduced expression of membrane proteins was not confined to those anchored through GPI--several transmembrane molecules including CD11a CD11b and CD18 were also reduced on apoptotic PMN, whilst other were little changed ( CD35, CD46). Paper-484291. Flow-cytometric analysis revealed that upregulation of CD46 occurred at a late stage of infection in both SupT1 cells and primary CD4+ T cells, and also that expression of another CRP, CD59, was increased at a late stage of infection. Paper-13173665. We have produced and tested the function of novel recombinant chimeric proteins that incorporate the functional domains of both CD46 (membrane cofactor protein, MCP) and the low affinity human IgG receptor FcgammaRII ( CD32). Paper-2191787. Because many viral and bacterial pathogens, including the major human pathogen Streptococcus pyogenes, bind to CD46, we asked whether this bacterium can directly induce Tr1-like cells through the streptococcal ligand for CD46, the M protein. Paper-10768337. Furthermore, we show that inhibition of STAT3- mediated CD46 cell surface expression sensitizes DU145 prostate cancer cells to cytotoxicity in an in vitro complement lysis assay using rabbit anti-DU145 antiserum and rabbit complement. Paper-13387598. Most human nucleated cells and cell lines possess C3 step regulators, decay-accelerating factor ( DAF; CD55) and membrane cofactor protein ( MCP; CD46) and an inhibitor of membrane attack complex (MAC) formation ( p18; CD59). Paper-38328. Adenovirus-mediated gene transfer of triple human complement regulating proteins ( DAF, MCP and CD59) in the xenogeneic porcine-to-human transplantation model. Part I: in vitro assays using porcine aortic endothelial cells. Paper-9162944. METHODS: Transgenic mice expressing a glycosylphosphatidyl inositol (GPI)-linked form of CD46 were generated by microinjection of a hybrid CD46/ CD55 cDNA under the control of the human intercellular adhesion molecule-2 promoter. Paper-1670369. Since the expression of the human MV receptor CD46 rendered the chicken embryonic cell line TCF more permissive to the infection by the Hallé MV strain, the MV entry into CEF appeared to be a limiting step in the absence of prior MV adaptation. Paper-1852514. Although Crry/ p65, like MCP and DAF, contains tandem short consensus repeats (SCR) characteristic of C3/ C4 binding proteins, Crry/ p65 is not considered to be a genetic homologue of either MCP or DAF. Paper-146059. Apparently, the alternative pathway convertase C3bBb is central for the pathophysiology of HUS as gene mutations of the components (C3 and Factor B) or of regulators ( Factor H, Factor I and MCP/ CD46) are observed in the genetic form of HUS. Paper-13602756. This structural unit is called SCR (short consensus repeat) and is found in the complement proteins C1r, C1s, C2, factor B, factor H, C4BP, DAF, MCP and CR2, each of which interacts with some cleavage products of C3 and/or C4. Paper-6062651. Of 114 total genes found overexpressed in common among all primary and metastatic tumors, 11 of 114 genes were located on chromosome 1q: ARF1, CD46, CNIH4, ENSA, FH, NICE-3, PSMB4, RGS2, RGS5, TIMM17A, and UFC1. Paper-12460063. Although the expression of CD46 and CD55 antigens were not differently expressed in the resistant cells, the complement inhibitor CD59 was overexpressed in a subpopulation and CD20 was found to be expressed at a lower level in a minority of cells. Paper-13599662. Increased vascular inflammation, including enhanced expression of interleukin- 6 ( IL-6), vascular cellular adhesion molecule-1 ( VCAM-1) and monocyte chemoattractant protein ( MCP- 1) are observed, as is a marked decrease in NO bioavailability. Paper-13352895. Further characterization showed that MCP compounds restore actin stress fibers and cause flat reversion in NIH 3T3 cells transformed with H-Ras (V12) but not in NIH 3T3 cells transformed with constitutively active Raf-1 (RafDeltaN). Paper-9173264. In contrast to human influenza A H1NI viruses, the H5N1 viruses hyper-induce cytokines (tumour necrosis factor [TNF]alpha, interferon beta) and chemokines ( IP10, MIP1alpha, MCP) in in vitro cultures of primary human macrophages. Paper-12421633. Therefore, these tg mice provide the opportunity for analyzing and comparing MV-T cell interactions and MV pathogenesis in cells expressing only the hSLAM MV receptor with those of tg mice whose T cells selectively express another MV receptor, CD46. Paper-9839564. During the MCP, the parabolic blood velocity profile was enhanced (p < 0.05) at higher contraction forces, 20 W during the CDP (ratio: 2.15-2.52) and 30 W during the CSP (ratio: 1.49-1.77), potentially because of a greater retrograde flow component. Paper-12226735. Here, using genetic approaches in mice, we demonstrate that TLX (also called NR2E1) regulates adult neural stem cell proliferation in a cell-autonomous manner by controlling a defined genetic network implicated in cell proliferation and growth. Paper-12751265. The structural gene for membrane cofactor protein ( MCP), a widely distributed C3b/C4b binding regulatory glycoprotein of the complement system, has been mapped to the same locus as the structural genes for CR1, CR2, DAF, and C4bp. Paper-6161596. CD46 exhibited the most striking pattern of association, with increased levels of expression being associated with ER-positive samples and lower levels of expression associated with a loss of differentiation and epidermal growth factor receptor positivity. Paper-10216153. Our data demonstrate that the native conformation of the CCP2 domain is crucial for Ad35 binding and that the substitution of amino acids at positions 130 to 135 or 152 to 156 completely abolishes the receptor function of CD46. Paper-10793492. We provide the first evidence of the expression of the 12 receptors CAR, CoupTFalpha, CoupTFbeta, FXR, GCNF, HNF4alpha, PPARbeta/delta, PXR, RevErbbeta, TR2, TR4 and TLX in highly purified CD4, CD8, CD19, CD14 cells. Paper-12364682. The transmembrane domain and cytoplasmic tail of CD46 were not required for receptor function as cells expressing the CD46 extracellular domain linked to the glycosyl-phosphatidylinositol tail of DAF were rendered susceptible to MV infection. Paper-181966. BACKGROUND: Although included in successive editions of the DSM since its introduction in the IIIrd, the subtyping of manic episode into 'with mood-congruent ( MCP) versus mood-incongruent ( MIP) psychotic features' is yet to be fully validated. Paper-12345333. An enzyme linked immunosorbent assay ( ELISA) has been developed for the quantitative determination of the most probable contaminating proteins ( MCP) of recombinant human Erythropoietin produced in the mouse fibroblast cell line C-127. Paper-6638487. Furthermore, to reach the site of injury, sequential responsiveness might operate, blood DC precursors are recruited by a set of chemokines ( MIP, MCP) while within the tissue other chemokines will direct their navigation ( CCL20/MIP-3alpha). Paper-10209256. This ESR peak intensity in DAp with collagen (c-DAp) decreased in an increase in the amount of collagen added into Ca(H2PO4)2H2O ( MCP) electrolytic solution, because dangling H+ of HPO(4)2- binds to the carboxyl group of collagen due to the hydrogen bond. Paper-8919212. Additionally, TLX finely tunes the progenitor differentiation program by modulating the phospholipase C and mitogen-activated protein kinase ( MAPK) pathways and the expression of an array of cell type-specific transcriptional regulators. Paper-12006573. The experimental observations regarding the function of CD9, CD49f/ CD29, CD46 and CD11b/ CD18 have led to the construction of general hypothesis of fertilization comprising the interaction of these CD molecules in binding and fusion of sperm and egg. Paper-13732429. The extended virus-binding surface of CD46 differs strikingly from those reported for the human virus receptor proteins CD4 and intercellular cell adhesion molecule-1 ( ICAM-1), suggesting that the CD46 structure utilizes a novel mode of virus recognition. Paper-1925588. Interleukin-1beta (IL-1beta) consistently downregulated the expression of CD46 and CD59; IL-4 consistently downregulated the expression of CD46 and transforming growth factor-beta1, consistently downregulated the expression of both CD46 and CD55. Paper-9728408. The influence of targeting measles virus (MV) to CD46 in the processing and presentation of MV H and NP to antigen specific MHC class II I-E(d)- and I-A(d)-restricted T cell hybridomas was explored using murine M12- CD46 B cell transfectants. Paper-7921199. The TLX/ HOX11 subfamily of divergent homeobox genes are involved in various aspects of embryogenesis and, in the case of TLX1/ HOX11 and TLX3/ HOX11L2, feature prominently as oncogenes in human T-cell acute lymphoblastic leukaemia. Paper-13280944. BACKGROUND: In both familial and sporadic atypical haemolytic-uraemic syndrome (aHUS), mutations have been reported in regulators of the alternative complement pathway including factor H ( CFH), membrane cofactor protein ( MCP), and the serine protease factor I (IF). Paper-11456596. Differences between species B1 and B2 Ads were documented with competition experiments based on anti- CD46 antibodies directed against epitopes flanking the putative Ad-binding sites, and with competition experiments based on soluble CD46 protein. Paper-12526983. The ability to activate cells via TLR2 by wild-type MV H protein is abolished by mutation of a single amino acid, asparagine at position 481 to tyrosine, as is found in attenuated strains, which is important for interaction with CD46, the receptor for these strains. Paper-9218640. These results show that activated STAT3 signaling induces the CD46 promoter and protects human cancer cells from complement-dependent cytotoxicity, suggesting a potential mechanism whereby oncogenic signaling contributes to tumor cell evasion of antibody-mediated immunity. Paper-13387598. The functional properties of the prostasome-bound MCP were assessed in comparison with a recombinant soluble form, gamma MCP1, which is composed of four short consensus repeats (SCR), type C of the serine/ threonine-rich domain ( STC), and unknown significance (UK). Paper-267998. The protein kinase A inhibitor SQ22536 blocked much of the PTH- induced stimulation of MCP activities, while dibutyryl cAMP stimulated it, suggesting that protein kinase A-dependent phosphorylation is important in PTH stimulation of proteasome activities. Paper-1517102. Here, we highlight new advances in the field, emphasising the interaction between MV proteins and their cellular targets, in particular the cell membrane receptors, CD46, CD150, TLR2 and FcgammaRII in the induction of immunological abnormalities associated with measles. Paper-11775142. Antigen-reactive B-lymphocytes can be activated to produce TA1- blocking antibodies by either allotypic TA2 ( TLX) in seminal plasma or allogeneic trophoblast membrane antigens during the host-versus-graft reaction subsequent to blastocyst implantation. Paper-5198906. The present study examined the influences of the muscle contraction ( MCP) and relaxation ( MRP) phases, as well as systole and diastole, on the blood velocity profile and flow in the conduit artery at different dynamic muscle contraction forces. Paper-12226735. METHODS: In this study, we assessed the capacity of brain epithelial cells to express membrane-bound complement regulators (ie, CD35, CD46, CD55 and CD59) in vitro and in situ by immunostaining of control and meningitis human brain tissue sections. Paper-12243064. To evaluate their role in GFND, we performed functional studies on RCA proteins in GFND patients from the large kindred, as well as mutational analysis of the genes for complement receptor-2 ( CR2), membrane cofactor protein ( MCP), and decay accelerating factor ( DAF). Paper-8645225. METHODS: We investigated the expression pattern of CD46, CD55, CD59, and CD97 (a receptor for CD55) in prostate cancer by tissue microarray analysis using high-density tissue microarrays spotted with tissue cores from biopsy and autopsy specimens. Paper-11499129. In this study we provide evidence that MCP on sperm that have undergone the acrosome reaction specifically binds dimeric C3b and that human sperm acrosomal proteases released during the acrosome reaction directly cleave C3, facilitating its binding to MCP. Paper-103837. METHODS AND RESULTS: In the normal esophageal mucosa, CD55 predominantly stained on the cell membrane of squamous epithelium in the superficial and prickle cell layers, whereas CD46 most intensely stained on the cell membrane in the basal and parabasal cell layers. Paper-10439547. In 25 cell lines (10 adenocarcinoma, 3 large-cell carcinoma, 7 small-cell lung cancer [ SCLC], and 5 squamous cell carcinoma), flow cytometric analysis revealed that MCP was expressed in all cell lines, whereas none of the cell lines was CR1-positive. Paper-7576932. Our data suggest that triggering of CD46 and associated protein kinases can lead to the induction of low levels of IL-6, whereas the replication of the negative strand RNA virus constitutes the major stimulus leading to the synthesis of high levels of IL-6 in astrocytes. Paper-1699618. Enhanced immunoperoxidase staining and radioimmunoassay confirmed that C regulators are differentially expressed on sperm; CD59 was strongly expressed on the surface of acrosome intact sperm while MCP and DAF appear to be located primarily on the inner acrosomal membrane. Paper-8222955. They participate the pathological process through the synthesis and secretion of pro-inflammatory cytokines, including interleukin 1 ( IL1), IL6, IL8, and the three colony stimulating factors G-CSF, M-CSF, and GM-CSF and the two chemotactic factors gro-alpha and MCP. Paper-525012. The time- and space-averaged, amplitude-weighted, mean (V(mean)) and maximum (V(max)) blood flow velocities were continuously measured in the common femoral artery during the cardiosystolic ( CSP) and cardiodiastolic (CDP) phases during MCP and MRP, respectively. Paper-12226735. Aggregation was independent of intercellular adhesion molecule-1 (ICAM-1)/lymphocyte function-associated antigen-1 ( LFA-1), but could be inhibited by monoclonal antibodies (mAb) against the MV hemagglutinin glycoprotein (MV-H). mAb against the MV receptor, CD46, also blocked aggregation. Paper-859495. As several reports have supported an association of HHV-6 and multiple sclerosis, it was of interest to compare levels of soluble CD46 in the sera of multiple sclerosis patients to that of healthy controls, other neurological disease controls, and other inflammatory disease controls. Paper-9076653. We investigated the constitutive and hypoxia-reoxygenation-dependent expression of complement receptor 1 ( CD35), membrane cofactor protein ( CD46), decay-accelerating factor ( CD55), protectin ( CD59), and complement C3a and C5a receptors ( C3aR and C5aR) on human NT2-N neurons. Paper-12432747. These findings suggest that ICAM1/ LFA1 interaction and MCP-mediated C3bi/C3biR interaction cooperate and participate in persistent glomerular infiltration of immune cells in immunoglobulin A nephropathy, and that these LFA1alpha+ and C3biR+ cells contribute to the induction of proteinuria. Paper-618589. Flow cytometry analysis demonstrated uptake of acetylated low density lipoproteins (Ac-LDL) and constitutive expression of SLA class I, CD29, CD31, CD41/61, CD80/86, CD46, SWC3, and LAMP-1 antigens by all analyzed lines and showed little differences to primary cells. Paper-9547177. Macrophage infiltration has been observed in the renal biopsy specimens of diabetic nephropathy ( DN), and hyperglycemic state stimulates the renal expression of RANTES (regulated upon activation, normal T-cell expressed and secreted) and MCP- 1 ( monocyte chemoattractant protein-1). Paper-13411653. OBJECTIVE: The aim of this study was to examine the presence of the complement regulatory proteins protectin ( CD59), decay accelerating factor ( CD55), membrane cofactor protein ( CD46) and clusterin (SP-40,40) in connection with the autoimmune exocrinopathy in Sjögren's syndrome (SS). Paper-1321948. RESULTS: Double immunofluorescence experiments for ependymal cell markers ( GFAP, S100, ZO-1, E-cadherin) and complement regulators indicated that the human ependymal cell line model was strongly positive for CD55, CD59 compared to weak stainings for CD46 and CD35. Paper-12243064. A novel ubiquitin fold modifier ( Ufm1) that has not been previously associated with ER stress and not found to be induced under any condition was also found to be upregulated in the hearts of MCP mice ( transgenic mice that express MCP-1 specifically in the heart). Paper-12170662. Here we demonstrate that adenovirus type 11 (Ad11; of species B) binds to Chinese hamster ovary (CHO) cells transfected with CD46 (membrane cofactor protein)-cDNA at least 10 times more strongly than to CHO cells transfected with cDNAs encoding CAR or CD55 (decay accelerating factor). Paper-9826073. The cleavage and activation of C3 exposes binding sites for factors B, H and I, properdin, decay accelerating factor ( DAF, CD55), membrane cofactor protein ( MCP, CD46), complement receptor 1 ( CR1, CD35) and viral molecules such as vaccinia virus complement-control protein. Paper-12308467. However, unequivocal staining with antibodies to two soluble complement regulatory proteins, S-protein and clusterin, and to the membrane complement inhibitor CD59 was found, although three other membrane inhibitors, CR1(CD35), DAF ( CD55), and MCP ( CD46), were not detected. Paper-541985. A natural killer (NK) target, K562 (a human erythroblastoid cell line), was found by flow cytometry to express three complement regulatory membrane proteins on its surface: decay-accelerating factor ( DAF, CD55), membrane cofactor protein ( MCP, CD46) and p18 ( CD59). Paper-44149. 1. We examined the effects of metoclopramide ( MCP: 10 mg i.v.) on plasma atrial natriuretic peptide (ANP) and aldosterone concentrations (PAC) and the effect of ANP on MCP-induced PAC in four patients with primary glomerular diseases and seven patients with essential hypertension. Paper-6921504. Dual colour microscopy with rhodamine-conjugated anti- vitronectin antibody and a fluorescein-conjugated antibody directed against CD46 (a complement regulatory protein expressed on the inner acrosomal membrane) revealed that only acrosome-reacted ( CD46-positive) spermatozoa displayed vitronectin. Paper-8528316. By RT-PCR, we found that complement activators of the classical pathway C1q C chain, C1r, C4, C3, as well as the complement regulators, C1 inhibitor, clusterin, MCP, DAF, CD59, were all expressed constitutively and at much higher level in HD brains compared to normal brain. Paper-8295780. Using fluorescent labeling, ultracentrifugation, and density gradient centrifugation, virtually all CD46 was present on prostasomes whereas CD59, CD55, AND CDw52 were also detected in a form which remained in the 200,000 g supernatant and equilibrated at higher density than prostasomes in gradients. Paper-516436. We will herein provide new emphasis on the role of neuroimmune regulatory proteins (NIRegs), such as CD95L, TNF, CD200, CD47, sialic acids, CD55, CD46, fH, C3a, HMGB1, which are involved in silencing innate immunity at the cellular and molecular levels and suppression of inflammation. Paper-13372752. We now describe the production and function of recombinant soluble CD46 (rsCD46), which was produced as a truncated form by mutagenesis using the splice overlap extension polymerase chain reaction, by inserting a translational stop codon into the CD46 cDNA at the junction of the transmembrane and extracellular domains. Paper-623863. Analysis by polymerase chain reaction, Northern blots, and transfection indicated that the predominance of MCP cDNA isoforms with STPB correlated with the high molecular weight protein phenotype, while the predominance of isoforms without STPB correlated with the lower molecular weight phenotype. Paper-6929001. Mutations in the complement regulators factor H, membrane cofactor protein ( MCP), and factor I are associated with atypical hemolytic uremic syndrome (aHUS, MIM 235400), suggesting that the disease develops as a consequence of the inefficient protection of the renal endothelium from damage by the complement system. Paper-11350212. Antigen uptake receptor expression ( mannose receptor, CD32, CD91, CD46) was decreased, and receptor-mediated endocytosis of fluorescein isothiocyanate-dextran was markedly impaired in rapa-DC, as were fluid phase endocytosis of Lucipher Yellow and phagocytic activity of bacteria and dead or apoptotic cells. Paper-9848369. In addition, our map shows the centromere-telomere orientation of the C4BPB/ MCP linkage group, which is: centromere-PFKFB2-C4BPB-C4BPA-SRP72-C4BPAL1++ +-C4BPAL2-telomere, and outlines an interval with a significant female-male recombination difference which suggests the presence of a female-specific hotspot(s) of recombination. Paper-993798. Previous studies have shown that human sperm that have undergone the acrosome reaction express a unique tissue-specific variant of the complement component 3 (C3)-binding molecule membrane cofactor protein ( MCP, CD46) and that damaged or dead sperm activate the alternative pathway of complement and bind C3 catabolites. Paper-103837. To evaluate the clinical usefulness of serum soluble CD46 as a marker of disease activity in patients with SLE, serum levels of sCD46 were measured by ELISA, using two MoAbs ( M160 and M177), each of which recognized two different epitopes on CD46 molecule in SLE, other autoimmune diseases and healthy controls. Paper-1873190. Differentiation of IMAC was disturbed in MCP-1-transfected MCS compared to experiments with non-transfected control aggregates, or the MIP-3alpha-transfected MCS, as indicated by high CD14 expression of MO/ IMAC cultured inside the MCP-1-transfected MCS, as shown by immunohistochemistry and FACS analysis. Paper-12046975. The results would indicate that the ARIC test as measured by flow cytometric analysis of CD46 binding is a sensitive and specific assay for use in the prediction of fertilization in IVF patients, thus enabling direct channelling of those patients with ARIC scores of < 10 into the more invasive micro-assisted fertilization schemes. Paper-861309. Using the MV-strain Edmonston (ED) and the recombinant MV-strain MGV in which the MV-envelope proteins H and F have been replaced by the vesicular stomatitis virus (VSV) envelope protein G, we investigated IL-6 induction in human U-251 astrocytoma cells in the presence and absence of a MV-specific receptor ( CD46) interaction. Paper-1699618. Altogether these data indicate that (a) MV targeting via CD46 has a strong effect on the efficiency of antigen presentation by MHC class II, (b) the effect is mediated by the binding of H to CD46, and (c) though MV does fuse with plasma membrane, endocytosis, and processing of virus particles are also occurring. Paper-7921199. CONCLUSIONS: We conclude that (1) hypoxia and reoxygenation of HUVECs significantly increases iC3b deposition on HUVECs, (2) C3 deposition after hypoxia and reoxygenation is largely mediated by the classic complement pathway, and (3) HUVEC-surface expression of CD46 and CD55 increases after hypoxia and reoxygenation. Paper-1098737. Recombinant soluble forms of the ectodomains of measles virus haemagglutinin (sH) and of its receptor CD46 (sCD46) were obtained as a purified disulphide-bonded sH homodimer with an apparent molecular mass of 160 kDa and a purified sCD46 monomer with an apparent molecular mass of 60 kDa, without detectable contamination with moesin. Paper-640635. AIMS: To investigate the deposition of complement components, C3d and C5b-9, and the expression of complement regulating factors ( S protein, membrane cofactor protein ( MCP; CD46), protectin ( CD59), decay accelerating factor ( DAF; CD55), and type 1 complement receptor ( CR1; CD35)) in gastric cancers. Paper-9210325. The model for the role of integrin CD11b/ CD18 and CD46 in fertilization is based on the interaction of these two molecules through complement C3 fragments which may serve as bridging ligands between sperm CD46 and oocyte CD11b/ CD18 and facilitate apposition of the sperm inner acrosomal membrane with oolemma. Paper-13732429. PROBLEM: To determine whether the results of the Acrobeads test, which measures the expression of the complement regulator molecule CD46 on the inner acrosomal membrane following the acrosome reaction, accurately identifies semen specimens that will exhibit reduced or failed fertilization following conventional IVF insemination. Paper-1057688. Based on these results, together with the fact that decay-accelerating factor ( DAF) serves as a factor for preventing C3 attack on human cells, we conclude that MCP and DAF cooperatively protect host cells from C3 targeting and, in these T cell lines, MCP is sufficient for preventing C3 deposition even without DAF. Paper-22837. On monocytes the expression of the Sialyl Lewis(x) antigen, the integrins CD11a, CD11c, and CD49d, and the complement-related markers CD46 and CD59 was higher in samples from uterine than from antecubital veins during preeclampsia, but not in uncomplicated pregnancies, whereas in nonpregnant women CD31 was decreased. Paper-9190271. Dot blot analysis showed the presence of mRNAs for all the complement components and regulatory proteins (C3, factor B, factor D, C5, C6, C7, C9, factor H, factor I, S-protein, SP-40, 40, DAF, MCP, CR1, CD59), except for properdin, C8 alpha, C8 beta and C8 gamma in all three types of synovial membrane studied. Paper-7800002. Since it has been suggested that MCPP- induced AVP release in animals is due to stimulation of 5HT1c receptors, the fact that MCPP did not induce the release of AVP in humans suggests that either MCPP is not a potent 5HT1c agonist or that AVP is not released by stimulation of 5HT(1c) receptors in human subjects. Paper-7309106. 2. Contractions to potassium chloride, 5-hydroxytryptamine and noradrenaline in rings of proximal parts of middle cerebral arteries ( MCP), surrounded with clot, and basilar arteries ( BAP), far from the clot, were examined 7 days after SAH, in which an autologous blood clot was bilaterally placed around major cerebral arteries. Paper-1933081. The capacity of arterial tissue to inhibit this activation through generation of the complement regulators C1 inhibitor, decay accelerating factor, membrane cofactor protein ( CD46), C4 binding protein ( C4BP), and protectin ( CD59) was evaluated in pairs of aortic atherosclerotic plaques and nearby normal artery from 11 human postmortem specimens. Paper-8850069. From January 1988 to August 1992, 18 patients (pts) with the established diagnosis of non-small cell lung cancer of the superior sulcus have been treated with a multidisciplinary approach, which includes 1-3 cycles of neoadjuvant chemotherapy ( MVP or MCP regimens) followed by simultaneous preoperative chemotherapy and external beam irradiation. Paper-8020957. METHOD OF STUDY: Human sperm membrane proteins were solubilized and subjected to polyacrylamide gel electrophoresis followed by Western blot analysis against antibodies to complement component 1 inhibitor ( C1-INH), decay-activating factor ( DAF; CD55), membrane cofactor protein ( MCP; CD46), and homologous restriction factor ( HRF; CD59). Paper-1422465. With this in mind, we have examined a new human colon cancer cell line (LIM1899) which has a heterogeneous expression of several cell surface molecules: by flow cytometry 38% were carcinoembryonic antigen positive; 64%, human milk fat globule positive, and 73%, CD46 positive; 87% of tumor cells bound a mixture of all three antibodies in vitro. Paper-6713634. In addition to this one-way cross-reaction with C4bp, a protein with a m.w. of approximately 60,000 ( p60) was found in two of three C4bp preparations that also cross-reacted with antiserum to MCP. p60 was present in trace quantities in the C4bp preparation and was successfully isolated from plasma by C3b affinity chromatography. Paper-6322. Human granulocytes (polymorphonuclear leucocytes, PMN) possess a membrane cofactor protein ( MCP, CD46), which is structurally and functionally distinct from the MCPs of other cell types: it shows a single broad band of 56-80 kDa (without the doublet pattern characteristic of MCP) on SDS/PAGE and has less affinity for complement component C3b. Paper-51931. These synonyms are used for gene CD46 (CD46 molecule, complement regulatory protein): Trophoblast leukocyte common antigen, TRA2.10, TLX, MIC10, MGC26544, Membrane cofactor protein, MCP, AHUS2. These accession numbers are used for gene CD46: Q15429 (UNIPROT__AC), BAF83916 (NCBI_GENBANK__AC), B4DL30 (UNIPROT__AC), AAF73844 (NCBI_GENBANK__AC). CD46 is a homologue of CD46 (CD46 molecule, complement regulatory protein) from Bos taurus. CD46 is a homologue of CD46 (CD46 molecule, complement regulatory protein) from Pan troglodytes. CD46 is a homologue of CD46 (CD46 molecule, complement regulatory protein) from Canis lupus familiaris. CD46 is a homologue of Cd46 (CD46 antigen, complement regulatory protein) from Mus musculus. CD46 is a homologue of Cd46 (CD46 molecule, complement regulatory protein) from Rattus norvegicus. Important links ! iHOP - Information Hyperlinked over Proteins . Concept & Implementation by Robert Hoffmann.