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CRP had no effect on PGI2 synthase ( PGIS) mass. Paper-10037051.
PGIS and TXAS have only 16% amino acid sequence identity. Paper-1391801.
Thus, the fallopian tube expresses both COX isoforms and PGIS. Paper-9152822.
Three functional candidate genes ( B4GALT5, KCNB1, and PTGIS) were sequenced. Paper-11216354.
Association of polymorphisms of PTGS2 and CYP8A1 with myocardial infarction. Paper-13688895.
RESULTS: Human oviductal smooth muscle expresses functionally active PGIS and IP. Paper-9594895.
A quantitative trait locus for SBP maps near KCNB1 and PTGIS in a population isolate. Paper-13779356.
We conclude that diabetes increases PGIS nitration in vivo, likely via dysfunctional eNOS. Paper-12288931.
Confocal analysis showed abnormal colocalization of PGIS and PGHS-1 to a filamentous structure. Paper-10583685.
A higher Ad- PGIS to Ad- COX-1 ratio caused a paradoxical decline in prostacyclin synthesis. Paper-10716246.
Resting ECV304 cells did not produce prostacyclin and had no detectable PGHS-1 and PGIS proteins. Paper-10583685.
The ratio of COX to PGIS activities was modified in HUVEC by treatment with interleukin-1beta (IL-1beta). Paper-8482273.
Human genes for prostaglandin endoperoxide synthase-2, thromboxane synthase and prostacyclin synthase. Paper-214547.
Furthermore, it also inhibits the platelet cyclooxygenase and lipoxygenase enzymes, and prostacyclin synthase. Paper-5805917.
Distribution of prostaglandin endoperoxide synthase and prostacyclin synthase in the late pregnant uterus. Paper-5209243.
Combined COX-1 and PGIS gene transfer has the potential for therapeutic augmentation of prostacyclin. Paper-10716246.
PGIS and PGHS-2 were also colocalized to ER in serum-treated or adenovirus-cyclooxygenase-2-infected cells. Paper-10583685.
Not prostacyclin synthase but prostaglandin endoperoxide synthase increases with human placental development. Paper-5449172.
The increased nitration and decreased activity of PGIS by CRP was reversed with peroxynitrite scavengers. Paper-10037051.
KW-3635 was inactive towards thromboxane synthase, cyclooxygenase and prostaglandin I2 synthase up to 10(-5) M. Paper-7497117.
Prostacyclin synthase and arachidonate 5-lipoxygenase polymorphisms and risk of colorectal polyps. Paper-11359112.
Conclusions: The CC genotype of CYP8A1 or the -765CC genotype of PTGS2 is associated with MI, respectively. Paper-13688895.
Thus, gene therapy with HGF and PGIS may be a promising strategy for severe pulmonary hypertension. Paper-10901929.
These findings underscore the importance of colocalization of PGHS and PGIS to ER and NE in prostacyclin synthesis. Paper-10583685.
Prostacyclin synthase gene transfer inhibits neointimal formation by suppressing PPAR delta expression. Paper-12611589.
Selective augmentation of prostacyclin production by combined prostacyclin synthase and cyclooxygenase-1 gene transfer. Paper-10716246.
Together with the PTGI, the patients also completed the Greek version of the Impact of Events Scale-Revised scale (IES-R-Gr). Paper-13035591.
Stimulation with 40-nM 17beta-estradiol significantly up-regulated protein and mRNA expression of both COX-1 and PGIS. Paper-10636419.
By increasing both superoxide and inducible NO synthase, CRP resulted in increased nitration of PGIS by peroxynitrite. Paper-10037051.
Improvement of survival of skin flaps by combined gene transfer of hepatocyte growth factor and prostacyclin synthase. Paper-12637429.
The subcellular colocalization of prostacyclin synthase ( PGIS) with prostaglandin H synthase ( PGHS) has not been delineated. Paper-10583685.
Western blot analysis revealed the expression of PGI synthase ( PGIS) and PGI receptor by fallopian tubes. Paper-9152822.
The mRNA steady-state levels of NOS-3, NOS-2, prostacyclin synthase, and COX- 1 were not altered by ischemia. Paper-8721977.
Pregnancy specifically increased COX-1 and PGIS protein expression in the myometrial tissues before labor (P < 0.01 for both). Paper-9577322.
In the vessel wall, PGIS and PGES seem to be major downstream enzymes in the endothelium and smooth muscle, respectively. Paper-10444852.
Immunohistochemical localisation of prostaglandin endoperoxide synthase and prostacyclin synthase in pregnant human myometrium. Paper-4867359.
The expression of prostacyclin synthase ( PGIS) and prostacyclin receptor ( IP) was confirmed by Western blot analysis. Paper-9594895.
We examined the expression of membrane-bound PGES and PGIS in myometrium from pregnant women during preterm and term labor. Paper-9577330.
All patient sera contained IgG4 antibodies against CYP8, CYP5A1 and the fungal mimic suggestive of continual antigenic challenge. Paper-1575499.
Insulin resistance reduces arterial prostacyclin synthase and eNOS activities by increasing endothelial fatty acid oxidation. Paper-11808855.
Increase in concentrations of prostaglandin endoperoxide synthase and prostacyclin synthase in human myometrium in late pregnancy. Paper-4794877.
These results indicate that co-transfer of HGF and PGIS genes by the Shima Jet could be an effective strategy to wound healing. Paper-12147309.
This implies the therapeutic potential of combined COX-1 and PGIS gene transfer in gene therapy for chronic renal diseases. Paper-10823684.
We recently reported that probably only COX and PGI-synthase ( PGIS) are involved in the biosynthesis of prostanoids in endothelial cells. Paper-8482273.
We observed that PGI synthase ( PGIS) mRNA levels were not modified by the exposure to IL-1beta, but the enzyme was partially inactivated. Paper-1529667.
Solution structure and topology of the N-terminal membrane anchor domain of a microsomal cytochrome P450: prostaglandin I2 synthase. Paper-9299829.
The combination of the structural information of and was able to simulate a solution structure of the unstable TXAS and PGIS substrate, PGH(2). Paper-8551705.
Western blot analyses revealed a predominant protein band of 180 kDa, and a second 16-kDa band for ir- PGES and 56-kDa band for ir- PGIS. Paper-9577330.
This may offer further evidence that PGH synthase rather than PGI synthase itself is the rate limiting factor in myometrial PGI2 production. Paper-4794877.
Double-label expression studies of prostacyclin synthase, thromboxane synthase and COX isoforms in normal aortic endothelium. Paper-12422642.
RESULTS: Common variation in TBXAS1 and PTGIS was associated with MI risk (p-value for global Chi-square test, 0.01 and 0.03, respectively). Paper-13513582.
The studies of PGIS with PA described herein provide a mechanistic model of a peroxidase reaction catalyzed by the class III cytochromes P450. Paper-13599363.
Cyclooxygenase, thromboxane A2 synthase, 12-lipoxygenase, prostacyclin synthase, plasma coagulation, and fibrinolysis were unaffected. Paper-10605714.
Prostaglandin I(2) synthase ( PGIS) is an eicosanoid-synthesizing cytochrome P450, located in the endoplasmic reticulum (ER) membrane. Paper-8370465.
Relative to its PGI synthase content pregnant myometrium contained twice as much PGH synthase as non-pregnant myometrium (p less than 0.01). Paper-4794877.
The results indicated that both LP1 peptides of PGIS and P450 2C1 became bound to the lipid bilayer, whereas both LP2 peptides did not bind the lipid. Paper-1391801.
In rat and human aortas, immunofluorescence studies also showed significant COX-1 and PGIS co-localization in the endothelium. Paper-12422642.
Furthermore, gene therapy with endothelial nitric oxide synthase gene or prostacyclin synthase gene may hold great promise in the treatment of PPH. Paper-10332277.
Human umbilical vein endothelial cell lysates were used to determine concentrations of the enzymes cyclooxygenase and prostacyclin synthase. Paper-180612.
Immunoreactive (ir-) PGES and PGIS proteins were localized to the cytoplasm of myocytes of the myometrium and vascular smooth muscle cells. Paper-9577330.
CONCLUSIONS: Prostacyclin synthesis can be selectively augmented by cotransfecting endothelial cells with an optimal ratio of COX-1 to PGIS. Paper-10716246.
PCS-like immunoreactivity was found in endothelial cells and leiomyocytes, whereas fetal and maternal macrophages showed positive staining for TXS. Paper-867226.
Prostacyclin synthase ( PGIS), a cytochrome P450 enzyme, catalyzes the biosynthesis of a physiologically important molecule, prostacyclin. Paper-931423.
Prostacyclin synthase and nitric oxide synthase, and cell cycle inhibitors, such as E2F decoy oligonucleotides (D-E2F), reduce neointima formation. Paper-9625677.
A stepwise forward selection procedure demonstrated that ITGA2, GCK, and PTGIS genotypes significantly affected the prevalence of hypertension. Paper-12292686.
Quantitative RT-PCR demonstrated increased expression of PGIS and IP receptor during the menstrual phase of the cycle compared with all other phases (P < 0.05). Paper-10268638.
Overexpression of prostacyclin synthase ( PGIS) decreases lung tumor multiplicity in chemical- and cigarette-smoke- induced murine lung cancer models. Paper-13548406.
PGI2 formation from prostaglandin H2 was not reduced, suggesting that cyclooxygenase activity, but not prostacyclin synthase, is affected by reoxygenation. Paper-6544943.
Western blot analysis confirmed the expression of COX-1, -2 and PGIS which were expressed by luminal epithelia and smooth muscle cells. Paper-10610794.
CONCLUSIONS: Thus, CRP decreases PGI2 release from HAECs by inactivating PGIS via nitration, additionally contributing to its atherogenicity. Paper-10037051.
Here, we report the crystal structure of human PGIS at 2.15 A resolution, which represents the first three-dimensional structure of a class III cytochrome P450. Paper-12319605.
Immunohistochemical distribution of renal prostaglandin endoperoxide synthase and prostacyclin synthase: diminished endoperoxide synthase in the hepatorenal syndrome. Paper-5785289.
This was established for the heme-thiolate ( P450) enzyme prostacyclin synthase which was tyrosine nitrated and inhibited at low PN levels [FEBS Lett. 382 (1996) 101]. Paper-8621588.
Characterization of the secondary structure and membrane interaction of the putative membrane anchor domains of prostaglandin I2 synthase and cytochrome P450 2C1. Paper-1391801.
By contrast, PGIS was not colocalized with PGHS-2 in cells induced with phorbol 12-myristate 13-acetate where PGHS-2 was visualized primarily in vesicle-like structures. Paper-10583685.
CONCLUSIONS: These results suggest that the genotypes for ITGA2, GCK, and PTGIS may prove reliable for the assessment of the genetic component of hypertension. Paper-12292686.
In a previous work, we postulated that endothelial cells possess only the following 2 enzymes involved in prostanoid synthesis: cyclooxygenase and prostacyclin synthase. Paper-8439852.
PGIS and PGHS-1 were colocalized to nuclear envelope (NE) and endoplasmic reticulum (ER) in resting and adenovirus-infected bovine aortic endothelial cells. Paper-10583685.
The expression of cyclooxygenase (COX)-1, COX-2 and PGI2 synthase ( PGIS) was analysed by western blot analysis and immunohistochemistry. Paper-10610794.
Results: The CC genotype of CYP8A1 and the -765CC genotype of PTGS2 were more common in the MI patients than in the control subjects (p=0.041, p=0.012, respectively). Paper-13688895.
Interestingly, the abundant PGIS and PGHS-1 expressed in adenovirus-infected ECV304 cells were colocalized to NE and ER, which synthesized a large quantity of prostacyclin. Paper-10583685.
Furthermore, PGIS and IP receptor were localised to the glandular epithelium, stromal and endothelial cells in the basal and functional layers of the endometrium. Paper-10268638.
Collectively, these results indicated that not only enhanced COX activity but also substantial PGIS inactivation was required for significant transcellular biosynthesis of TxA(2). Paper-8482273.
The level of PPAR delta expression was lower in Ad- PGIS-treated arteries than in control vessels, with the PPAR delta localized in the neointima adjacent to endothelium. Paper-12611589.
Although both COX isoforms are comparably expressed, prostacyclin synthase expression is higher in GN WAT, with levels of activity correlating directly with IL-6. Paper-13505503.
In dogs, COX-2 expression was found to be restricted to small foci of endothelial cells while COX-1, PGIS and TXS were widely distributed throughout the endothelium. Paper-12422642.
The 11 beta-hydroxysteroid dehydrogenase gene, the prostacyclin synthase gene, genes coding for variants in G proteins, and adrenergic receptor genes have received particular attention. Paper-8441610.
PGIS was heavily colocalized with PECAM-1 to endothelial cells at the leptomeninges, large and small vessels, and localized to neuronal cells, largely at the penumbra area. Paper-11399900.
Prostacyclin synthase ( PCS) and thromboxane synthase ( TXS) and their mRNA's were localized by immunohistochemistry using monoclonal antibodies and in situ hybridization. Paper-867226.
Staining associated with prostacyclin synthase, however, was more intense throughout the myometrium and less circumscript than that associated with prostaglandin endoperoxide synthase. Paper-4867359.
17beta-estradiol up-regulates prostacyclin production in cultured human uterine myometrial cells via augmentation of both cyclooxygenase-1 and prostacyclin synthase expression. Paper-10636419.
Prostacylin (PGI(2)), one of the major prostaglandins, is derived from arachidonic acid by the action of the cyclooxygenase ( COX) system coupled to PGI(2) synthase ( PGIS). Paper-9220144.
Immunohistochemistry confirmed the expression of PGIS by luminal epithelia, tubal smooth muscle, vascular endothelial cells, and vascular smooth muscle cells. Paper-9152822.
While notable sequence divergence has been recognized between PGIS and other P450s, PGIS exhibits the typical triangular prism-shaped P450 fold with only moderate structural differences. Paper-12319605.
Activities of phospholipase A2 (PHA2), cyclooxygenase ( PGHS), and PGI2 synthetase ( PGIS) or TXA2 synthetase ( TXAS) were determined in platelets and in endothelial cells. Paper-7189116.
Treatment of cultured predecidualized stromal cells with S1P resulted in upregulation of Ptgs2 mRNA and PTGS2 protein, but not the downstream enzyme prostacyclin synthase. Paper-11345201.
Prostacyclin synthase ( PGIS) is a membrane- bound class III cytochrome P450 that catalyzes an isomerization of prostaglandin H(2), an endoperoxide, to prostacyclin. Paper-13599363.
Differences in prostacyclin production under the two experimental conditions could be explained neither by differences in enzyme mass nor activities of cyclooxygenase and prostacyclin synthase. Paper-180612.
CONCLUSIONS: Study results suggest that variation in TBXAS1 and PTGIS may influence MI risk, and carriers of rs20417C allele might derive greater benefits from aspirin use in primary prevention. Paper-13513582.
Our topology studies have indicated that the N-terminal region of TXAS has a longer N-terminal endoplasmic reticulum (ER) membrane anchor region compared with the same segment proposed for PGIS. Paper-8551705.
CONCLUSIONS: Overall, these results indicate that combination treatment with HGF and PGIS genes by Shima Jet could be an effective strategy to improve skin flap survival. Paper-12637429.
CONCLUSIONS: Ad- PGIS gene transfer reduced PPAR delta expression and inhibited neointimal formation after balloon injury in accordance with the reduction in the phosphorylation of p38 MAPK. Paper-12611589.
Prostacyclin synthase ( PGIS) and arachidonate 5-lipoxygenase ( ALOX5) are enzymes relevant to prostaglandin and leukotriene synthesis, both important pathways for colon cancer risk. Paper-11359112.
In summary, these results point to a common role of the thiolate ligand in the oxygen activation mechanism by thromboxane and prostacyclin synthase and liver cytochrome P-450 monooxygenases. Paper-5760655.
To determine the role of this receptor in lung cancer chemoprevention by prostacyclin, PGIS-overexpressing mice were crossed to mice that lack the IP receptor [ IP(-/-)]. Paper-13548406.
The IL-1beta-induced increase in the release of PGH2 by HUVECs was suppressed by the COX-2-selective inhibitor SC-58125 and correlated with both COX-2 expression and PGIS inactivation. Paper-1529667.
To study the protective effect of prostacyclin ( PGI2) we increased PGI2 production by infected NRK-52E cells with an adenovirus carrying cyclooxygenase-1 and prostacyclin synthase. Paper-12742547.
Western blot analysis detected cPLA(2) and COX-1 and PGIS protein expression in the cultured human myometrial cells; however, COX-2 protein expression was below the detection sensitivity. Paper-10636419.
TXAS and prostaglandin I(2) synthase ( PGIS), respectively, convert the same substrate, prostaglandin H(2) (PGH(2)), to thromboxane A(2) and prostaglandin I(2), which have opposite biological functions. Paper-8551705.
Prostacyclin synthase ( PGIS), which catalyzes the conversion of prostaglandin (PG) H(2) to prostacyclin (PGI(2)), is a member of the cytochrome P-450 ( P450) superfamily, CYP8A1. Paper-10451337.
Prostacyclin synthase ( PGIS) is a cytochrome P450 ( P450) enzyme that catalyzes production of prostacyclin from prostaglandin H(2). Paper-12706433.
Heparin and acidic fibroblast growth factor interact to decrease prostacyclin synthesis in human endothelial cells by affecting both prostaglandin H synthase and prostacyclin synthase. Paper-6537008.
According to the sequence of 26 amino acids from the N-terminus and two tryptic peptides no homology to one of the cytochrome P450 monooxygenases, or to cyclooxygenase, or to prostacyclin synthase was detected. Paper-6818118.
The cDNA for human prostacyclin synthase was cloned by polymerase chain reaction using poly(A)+ RNA from human aortic endothelial cells according to the partial nucleotide sequence of prostacyclin synthase gene. Paper-8080192.
CONCLUSION: 17beta-Estradiol from placenta may contribute to the augmentation of PGI(2) production in the human myometrium in the middle of gestation via up-regulation of both COX-1 and PGIS expression. Paper-10636419.
Abstract Background: Cyclooxygenase-2 ( COX-2) and prostacyclin synthase ( PGIS) are enzymes involved in prostaglandin and prostacyclin synthesis, which have been linked to cardiovascular disease risk. Paper-13688895.
The localisation of prostaglandin endoperoxide synthase and prostacyclin synthase in pregnant human myometrium was examined by indirect immunocytofluorescent staining using monoclonal antibodies raised against these enzymes. Paper-4867359.
The effect of AA on EPA conversion to minor prostaglandins like PGE3 and PGF3 alpha was similar then confirming the stimulating effect and suggesting it is occurring at the cyclooxygenase instead of the prostacyclin synthase level. Paper-5428491.
Topics discussed included current clinical therapies and novel therapeutic strategies, e.g., nitric oxide, phosphodiesterase (PDE) inhibition, prostacyclin synthase expression, endothelin receptor antagonism and elastase inhibition. Paper-11781862.
FFA-induced overproduction of superoxide activated a variety of proinflammatory signals previously implicated in hyperglycemia-induced vascular damage and inactivated 2 important antiatherogenic enzymes, prostacyclin synthase and eNOS. Paper-11808855.
In contrast, expression of inducible (nitric oxide synthase-2) or constitutive endothelial (nitric oxide synthase-3) nitric oxide synthase, prostacyclin synthase, and constitutive cyclooxygenase ( COX-1) were not altered after CP-Rep. Paper-2036053.
Over that period, wherein trophoblastic invasion of the uterine spiral arteries occurs, the placentae showed a significant increase in concentrations of PGH synthase (r = 0.73, p less than 0.001; n = 20), but not in those of PGI synthase. Paper-5449172.
CONCLUSION: Specific PTGIS and TBXAS1 variants may affect breast cancer susceptibility, but common variants in PTGS1, PTGS2, PTGES, PTGDS, and PGDS have no major role in breast cancer susceptibility. Paper-13667737.
The observed increased expressions of endothelial COX-1, prostacyclin synthase, thromboxane synthase and enhanced TP receptor sensitivity are not prerequisites for, but intensify the magnitude of endothelium-dependent contractions. Paper-13724437.
The peptide sequence showed a 39% similarity with human prostacyclin synthase ( CYP8) and 31% similarity with the rate-limiting enzyme in over-all synthesis of bile acids, the cholesterol 7alpha-hydroxylase ( CYP7) of the rabbit. Paper-817059.
While the variation between individual placentae was much larger for PGI synthase than for PGH synthase concentrations, there was no evidence for a large excess of PGI synthase over that of PGH synthase in any of these early placentae. Paper-5449172.
BACKGROUND: We tested the hypothesis that combined cyclooxygenase-1 ( COX-1) and prostacyclin synthase ( PGIS) gene transfer selectively augments prostacyclin production without a concurrent overproduction of other prostanoids. Paper-10716246.
Thus, we succeeded in obtaining a sufficient amount of the purified recombinant human PGIS from infected insect cells for spectral analyses that has high specific activity and the characteristics of a P450, indicating substrate specificity. Paper-10451337.
Oxidative stress may drive this imbalance because lipid peroxides activate the cyclooxygenase enzyme to increase thromboxane synthesis, but at the same time they inhibit prostacyclin synthase to decrease prostacyclin synthesis. Paper-10344379.
Quantification of the total cross-sectioned aortic endothelium revealed a 6- to 7-fold greater expression of COX-1 relative to COX-2 (55 vs. 8%) and greater co-distribution of PGIS with COX-1 compared to COX-2 (19 vs. 3%). Paper-12422642.
Myometrial microsomes from pre-eclamptic women contained ten times more PGI synthase than PGH synthase and the ratio of PGI synthase to PGH synthase (mean +/- SD; 10.1 +/- 3.9) was not different from that in normotensive pregnancies. Paper-4878520.
Overall, our data support a role for genetic variations in transcription factor AP-2 beta, tumor necrosis factor receptor-associated factor 1, and prostacyclin synthase in the persistent patency of the ductus arteriosus seen in preterm infants. Paper-13696905.
Our results show that optimal ratios of adenoviral titers to achieve a large prostacyclin augmentation without overproduction of prostaglandin E2 or F2alpha were 50 to 100 plaque forming units (pfu) of Ad- COX-1 to 50 pfu of Ad- PGIS per cell. Paper-10716246.
These results support the hypothesis that PGIS and TXAS interact with the endoplasmic reticulum membrane in different ways, in which the NH2-terminal anchor domain of PGIS, as with P450 2C1, appears to have a single transmembrane segment. Paper-1391801.
In a Minnesota-based case-control study of adenomatous (n = 517) or hyperplastic (n = 192) polyps versus polyp-free controls (n = 618), we investigated the role of promoter repeat polymorphisms in PGIS and ALOX5 as well as ALOX5 -1700 G>A. Paper-11359112.
Next, we determined the optimal ratio of Ad- COX-1 to Ad- PGIS by transfecting human umbilical vein endothelial cells with various titers of these 2 adenoviral constructs and determined the level of protein expression and prostanoid synthesis. Paper-10716246.
To test the hypothesis that its colocalization with PGHS is crucial for prostacyclin synthesis, we determined subcellular locations of PGIS, PGHS-1, and PGHS-2 in bovine aortic endothelial cells by immunofluorescent confocal microscopy. Paper-10583685.
Incubation of human umbilical vein endothelial cells (HUVECs) with human recombinant CRP (5 microg mL(-1)) suppressed PGI2 synthase expression and significantly increased thromboxane receptor levels. Paper-13929436.
Here, we report that intracellular prostacyclin formed by expressing prostacyclin synthase in human embryonic kidney 293 cells promotes apoptosis by activating endogenous peroxisome proliferator-activated receptor delta ( PPAR delta). Paper-8863842.
The expression of prostacyclin synthase was by far the most abundant, explaining why the majority of the COX-1-derived endoperoxides are transformed into prostacyclin, substantiating the role of prostacyclin as an endothelium-derived contracting factor. Paper-12749511.
The Sf-9 cells' membrane fraction, rich in TriCat enzyme, exhibited strong TriCat functions (K(m)=3muM and K(cat)=100molecules/min) for the TriCat enzyme and was 3-folds faster in converting AA to PGI(2) than the combination of the individual COX-2 and PGIS. Paper-13091304.
These findings indicate that PGIS protects the brain by enhancing PGI(2) synthesis and creating a favorable PGI(2)/TXA(2) ratio.Journal of Cerebral Blood Flow & Metabolism (2006) 26, 491-501. doi:10.1038/sj.jcbfm.9600205; published online 10 August 2005. Paper-11399900.
Hypertension augmented the expression of COX-1, prostacyclin synthase, thromboxane synthase, and hematopoietic-type prostaglandin D synthase in endothelial cells and prostaglandin D(2) ( DP), EP(3), and EP(4) receptors in SMC. Paper-12749511.
We have performed double-label immunofluorescence microscopy studies to evaluate the extent of co-localization of prostacyclin synthase ( PGIS) and thromboxane synthase (TXS) with cyclooxygenase (COX)-1 and COX-2 in normal aortic endothelium. Paper-12422642.
Combined genotype analysis of these polymorphisms yielded a lowest odds ratio of 0.47 for the genotypes of AA or AG for ITGA2, GA or AA for GCK, and CC for PTGIS, which were present in 1.1% and 2.0% of hypertensive and control individuals, respectively. Paper-12292686.
In conclusion, this study has confirmed increased expression and signalling of PGIS and IP receptor during the menstrual phase and outlines a potential autocrine/paracrine role for PGI(2) on several cellular compartments in the endometrium including the endothelium. Paper-10268638.
METHODS: The steady state transcript levels for eNOS and two other shear stress regulated genes (angiotensin-converting enzyme [ACE] and prostacyclin synthase [PGI2S]) were measured in samples of skeletal muscle from patients with CHF before and after 12 weeks of training. Paper-9012189.
To characterize the membrane anchor function of the PGIS NH2-terminal region, we have used the peptidoliposome reconstitution assay to identify the membrane anchor segment in the PGIS NH2-terminal domain and compared it with the anchor segment of P450 2C1. Paper-1391801.
It also led us to conclude that the PGIS substrate, prostaglandin H(2) (PGH(2)), produced by prostaglandin H(2) synthase ( PGHS) in the ER lumenal side must pass through the ER membrane barrier to the catalytic site of the PGIS in the cytoplasmic side of the ER membrane. Paper-8370465.
In a previous paper we reported that arachidonic acid (20:4(n-6] strongly enhances the endothelial cell synthesis of prostaglandin I3 (PGI3) from eicosapentaenoic acid (20:5(n-3], in stimulating the cyclooxygenase rather than the prostacyclin synthase (Bordet et al. Paper-5789864.
Our results also underline the importance of PTGIS and PPARD in the trophoblast and PTGIR in the uterus, suggesting that PGI2 is of both uterine and trophoblastic origin and is involved in a complex signalling pathway at around the time of implantation in the ewe. Paper-11838934.
Four peptides, mimicking putative NH2-terminal membrane anchor segments of PGIS and P450 2C1, containing residues 1-28 (PGIS-LP1 and P450 2C1-LP1) or residues 25-54 (PGIS-LP2 and P450 2C1-LP2), were synthesized and their ability to insert in a lipid bilayer was evaluated. Paper-1391801.
Here we review the findings obtained in these studies regarding CYP1A1, CYP1A2, CYP1B1, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C18, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, CYP3A7, CYP8A1 and CYP21 gene polymorphisms. Paper-10450094.
These results provided the first experimental evidence to localize the membrane contact residues in the F/G loop region of microsomal P450 and are valuable to further define and understand the membrane topology of PGIS and those of other microsomal P450s in the native membrane environment. Paper-9823125.
Prostaglandin endoperoxide synthase (i.e. cyclooxygenase; PGH synthase) and prostacyclin synthase ( PGI synthase) were quantitated with specific immunoradiometric assays in microsomes from human placentae (n = 20) obtained from 7 up to 17 weeks of gestation. Paper-5449172.
On the other hand, the much larger increase in PGH synthase than in PGI synthase in pregnant as compared to non-pregnant myometrium, may serve to promote preferential synthesis of prostaglandins that are potent myometrial stimulants and of critical importance in human parturition. Paper-4794877.
To evaluate possible causes of the diminished prostaglandin production in advanced hepatorenal syndrome, prostaglandin endoperoxide synthase and prostacyclin synthase were localized and semiquantitated by immunofluorescence in postmortem, biopsy and nephrectomy renal tissues. Paper-5785289.
Methods: In a Chinese case control study of MI patients (n=356) and healthy controls (n=350), we investigated the roles of polymorphisms in the PGIS gene ( CYP8A1) and the COX-2 gene ( PTGS2) using polymerase chain reaction-restriction fragment length polymorphism analysis. Paper-13688895.
The aim of this study was to elucidate how endogenous PGI(2) overexpression affects the expressions of PPAR delta and mitogen-activated protein kinases (MAPKs) in the development of neointimal formation in experimental angioplasty with adenovirus-mediated PGI(2) synthase (Ad- PGIS) gene transfer. Paper-12611589.
This study was designed to outline further the role of PGI(2) in menstruation by investigating the temporal pattern and site of expression of prostaglandin I synthase ( PGIS) and the prostacyclin receptor ( IP receptor) in the non-pregnant human endometrium across the menstrual cycle. Paper-10268638.
Carcinogen-induced lung tumor incidence was similar in IP(+/+), IP(+/-), and IP(-/-) mice, and overexpression of PGIS gave equal protection in all three groups, indicating that the protective effects of prostacyclin are not mediated through activation of IP. Paper-13548406.
Incubation of uterine arteries with highly purified hCG resulted in a dose-dependent increase in immunoreactive cyclooxygenase-1, cyclooxygenase-2, prostacyclin synthase, and 6-keto-prostaglandin-F1 alpha and a decrease in prostaglandin-E2, thromboxane-A2 synthase, and thromboxane-B2. Paper-8067194.
To clarify the mechanism in PGI(2) secretion from the myometrium, we first investigated the protein expression of cytosolic phospholipase A(2), cyclooxygenase (COX)-1, COX-2, and prostacyclin synthase ( PGIS) in the human uterine myometrium at various gestational ages before labor. Paper-9577322.
Immunoblotting with patient sera and fragments of each candidate autoantigen expressed as Escherichia coli gene 10 fusion proteins confirmed CYP8 and CYP5A1 as possible antigens, and revealed the presence of IgG1 and IgG3 antibodies against a construct mimicking fungal CYP52A10. Paper-1575499.
Hydropathy analysis suggests that the putative NH2-terminal membrane anchor domain of PGIS is similar to many other membrane-bound microsomal P450s, which are believed to be anchored by a single transmembrane segment, and thus different from the TXAS anchor, which appears to have two transmembrane segments. Paper-1391801.
Concentrations of prostaglandin endoperoxide synthase (i.e. cyclooxygenase; PGH synthase) and prostacyclin synthase ( PGI synthase) were quantified with specific radioimmunometric assays in human myometrium during the last trimester of pregnancy (n = 23) and in non-pregnant controls (n = 8). Paper-4794877.
The nearest neighbors to the P45014DM cluster in the phylogenetic tree were CYP7 ( cholesterol 7 alpha-hydroxylase) and CYP8 ( prostacyclin synthase), and the divergence point of fungal and mammalian P45014DMs was clearly more recent than that of P45014DM and CYP7/ CYP8. Paper-928343.
At 3 days before operation, 400 microg of human HGF and PGIS naked plasmid DNA or control plasmid was transfected into the flaps by needle-less injection using a Shima Jet, resulting in successful expression of human HGF and PGIS in the skin flaps. Paper-12637429.
RESULTS: Multivariable logistic regression analysis with adjustment for age, sex, body mass index, and the prevalence of smoking revealed that four polymorphisms (1648G-->A in ITGA2, -30G-->A in GCK, A-->G in SAH, and 1117C-->A in PTGIS) were significantly (P < .01) associated with hypertension. Paper-12292686.
We focused on a combined strategy to stimulate not only angiogenesis, but also vasodilation of local microvessels, using co-transfection of the hepatocyte growth factor ( HGF) and prostacyclin synthase ( PGIS) genes to enhance the survival of random-pattern skin flaps. Paper-12637429.
Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta ( PPARD) and retinoid x receptor alpha ( RXRA) in rat endometrium during artificially-induced decidualization. Paper-13524177.
EXPERIMENTAL DESIGN: DNA samples from 9,030 cases and controls were genotyped for 64 single nucleotide polymorphisms tagging known common variants (minor allele frequency > 0.05) in PTGS1, PTGS2, TBXAS1, PTGIS, PTGES, PTGDS, and PGDS with a two-stage case-control study design. Paper-13667737.
Furthermore, SQ29548, a thromboxane/ prostaglandin (PG) H(2) ( TP) receptor antagonist, significantly reduced the increased endothelial cell apoptosis and the expression of soluble intercellular adhesion molecule-1 that occurred in cells exposed to high glucose, without affecting the decrease in PGIS activity. Paper-9349868.
Furthermore, diabetes significantly suppressed PGIS activity in parallel with increased superoxide and PGIS nitration in the aortas of diabetic C57BL6 mice but had less effect in diabetic mice either lacking eNOS or overexpressing human SOD ( hSOD(+/+)), suggesting an eNOS-dependent PGIS nitration in vivo. Paper-12288931.
Concurrent administration of polyethylene-glycolated superoxide dismutase (SOD), l-nitroarginine methyl ester, or sepiapterin not only reversed the effects of high glucose on both angiotensin II-induced relaxation and PGI(2) release but also abolished high-glucose-enhanced PGIS nitration, as well as its association with eNOS. Paper-12288931.
The odds ratio (OR) estimated by the combined analysis for the CYP8A1 CC and PTGS2 -765CC genotypes [OR=5.44; 95% confidence interval (CI): 3.12-7.23] was markedly higher than that estimated separately for the CYP8A1 CC genotype (OR=1.37; 95% CI: 0.95-2.85) or the PTGS2 -765CC genotype (OR=2.92; 95% CI: 1.78-5.76) alone. Paper-13688895.
To investigate the protective effect of PGI(2) on cells undergoing adriamycin-induced apoptosis, this study selectively augmented PGI(2) production via adenovirus-mediated transfer of genes for cyclooxygenase-1 ( COX-1) and prostacyclin synthase ( PGIS) (two key enzymes of PGI(2) synthesis) to renal tubular cells. Paper-10823684.
This study investigated the effects of shear stress on gene expression of prostacyclin synthesis-related enzymes cyclooxygenases ( COX-1 and COX-2), prostacyclin synthase ( PGS), and thromboxane synthase ( TXS) and their metabolites prostaglandin (PGI(2)) and thromboxane A(2) (TXA(2)) in endothelium of intact conduit vessels. Paper-2148251.
Uterine prostacyclin synthase ( PGI synthase) and prostaglandin endoperoxide synthase ( PGH synthase) concentrations, measured by specific immunoradiometric assays, did not differ between patients with severe pregnancy-induced hypertension (syn. pre-eclampsia; n = 5) and normotensive gravidae (n = 6) with comparable gestational ages (34 - 38 weeks). Paper-4878520.
Ad- COX-1/ PGIS transfection was found to reduce the adriamycin-stimulated activities of caspase-3 and caspase-9, inhibit adriamycin-induced release of cytochrome c, elevate the expression of Bcl-x(L), and suppress the activation and translocation of nuclear factor-kappaB ( NF-kappaB) in adriamycin-treated renal tubular cells. Paper-10823684.
These synonyms are used for gene PTGIS (prostaglandin I2 (prostacyclin) synthase): PTGI, Prostaglandin I2 synthase, Prostacyclin synthase, PGIS, MGC126860, MGC126858, CYP8A1, CYP8.
These accession numbers are used for gene PTGIS: Q9HAX4 (UNIPROT__AC), Q3MII8 (UNIPROT__AC), CAC14162 (NCBI_GENBANK__AC), AAG31781 (NCBI_GENBANK__AC).
PTGIS is a homologue of ptgisl (prostaglandin I2 (prostacyclin) synthase like) from Danio rerio.
PTGIS is a homologue of PTGIS (prostaglandin I2 (prostacyclin) synthase) from Bos taurus.
PTGIS is a homologue of PTGIS (prostaglandin I2 (prostacyclin) synthase) from Pan troglodytes.
PTGIS is a homologue of PTGIS (prostaglandin I2 (prostacyclin) synthase) from Canis lupus familiaris.
PTGIS is a homologue of Ptgis (prostaglandin I2 (prostacyclin) synthase) from Mus musculus.
PTGIS is a homologue of Ptgis (prostaglandin I2 (prostacyclin) synthase) from Rattus norvegicus.
Important links !
iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.