The most recent information on SQSTM1 is here. Click here for the function of SQSTM1. Edit this page in Wiki Genes - SQSTM1 or see Wiki Gene. p62 functions as a p38 MAP kinase regulator. Paper-2167695. These inclusions were also positive for p62 but not for TDP-43. Paper-12517164. The myoglobin of the killer whale ( Orcinus orca). Paper-2692635. Mutations of the SQSTM1/ p62 gene are commonly observed in PDB. Paper-12415738. The mRNA expression of ZIP1 and ZIP3 in RWPE1 and RWPE2 was comparable. Paper-11774634. We have previously shown that p62/ SQSTM1 binds to p38. Paper-12856023. Total DDT was highest (28.4 ppm) in a neonatal Orcinus orca. Paper-8134701. In brain of p62 knock-out mice we did not recover polyubiquitinated TRAF6. Paper-10974223. Evidence for involvement of p62 in MEK5- ERK5 signaling is presented. Paper-9977887. Sequestosome 1/ p62 shuttles polyubiquitinated tau for proteasomal degradation. Paper-11262553. This study revealed genetic associations of SLC1A4, SQSTM1, and EIF4EBP1 with MSA. Paper-12876046. Age-associated oxidative damage to the p62 promoter: implications for Alzheimer disease. Paper-13596763. It is proposed that the 5q35-qter and 5q31 loci be named " PDB3" and " PDB4," respectively. Paper-9036347. PSI treatment abrogates the PDEF- stimulated increase of p62 promoter activity by 50%. Paper-9881197. Two isoenzymes of this activity ( p45 and p60) were identified by chromatography. Paper-907228. Specific regulation of cytokine-dependent p38 MAP kinase activation by p62/ SQSTM1. Paper-12856023. The p60 protein ( IRAK-4) was the earliest component of the complex to be phosphorylated. Paper-13418962. Furthermore, p62 interacts both with MEK5 and NBR1 in addition to the aPKCs. Paper-9977887. Reassignment of residue 122 in the myoglobin from the killer whale, Orcinus orca. Paper-3905505. The N-terminus and Phe52 residue of LC3 recruit p62/ SQSTM1 into autophagosomes. Paper-12963937. This approach revealed that p62- and LC3-positive bodies are degraded in autolysosomes. Paper-13385268. Strikingly, p62 and LC3 formed a shell surrounding aggregates of mutant huntingtin. Paper-11504287. To date, five L. monocytogenes autolysins have been identified: p60, P45, Ami, MurA and Auto. Paper-10638828. In both infected and uninfected cells, p60 was found in a complex with the cdc2 protein kinase. Paper-6386542. DNA ploidy, c-myc oncoprotein p62 and v-H-ras oncoprotein p21 in colorectal carcinoma. Paper-86753. Our analyses demonstrate that the XPB, XPD, p44, and p62 proteins interact with each other. Paper-569429. The p62 subunit did not interact with the related copper ATPase, ATP7A. Paper-12004925. Sequestosome 1 ( SQSTM/ p62) has been identified as the causative PDB gene in this region. Paper-10469660. During S phase, p150 and p60 are concentrated at sites of intranuclear DNA replication. Paper-1472889. The human p62 cDNA encodes Sam68 and not the RasGAP- associated p62 protein. Paper-467643. The most frequently expressed was c-myc p62 in 70% of cases followed by ras p21, 55% and c-erbB-2, 35%. Paper-108. Functional analysis of nuclear pore complex protein Nup62/ p62 using monoclonal antibodies. Paper-12008048. No SQSTM1/ p62 coding mutations were found in the remaining 9 families or in 113 age-matched controls. Paper-10469660. Antibodies to p62 ( sequestosome 1) showed novel oligodendroglial inclusions in the motor cortex. Paper-11986936. Recurrent mutation of the gene encoding sequestosome 1 ( SQSTM1/ p62) in Paget disease of bone. Paper-9161805. Three novel missense mutations of SQSTM1 were identified in familial PDB, all affecting the UBA domain. Paper-10457260. Immediate early response of the p62 gene encoding a non-proteasomal multiubiquitin chain binding protein. Paper-1651957. We identified the atypical protein kinase C ( aPKC) scaffold protein p62 as an Ajuba binding partner. Paper-10764634. Associations between multiple system atrophy and polymorphisms of SLC1A4, SQSTM1, and EIF4EBP1 genes. Paper-12876046. Also, p62/ SQSTM1 binds directly to LC3 and GABARAP family proteins via a specific sequence motif. Paper-13607611. Immunocytochemical analyses were performed for p65, Gab2, sequestosome 1/ p62, and ubiquitin. Paper-12282872. We propose that NBR1 and p62 act as receptors for selective autophagosomal degradation of ubiquitinated targets. Paper-13642325. ( p62). p62 corresponds to a polypyrimidine tract-binding protein recently described by Garcia-Blanco et al. Paper-19977. In this manner, several novel TAAs including HCC1, p62, p90, and others have been identified. Paper-13726955. Mutations in the UBA domain of SQSTM1 are a common cause of Paget's disease of bone. Paper-11237867. IHBs observed in the hepatocellular carcinoma cells presented are the first indications of a role of p62 in disease. Paper-1898054. Similarly, p62 immunoreactivity colocalizing with desmin aggregates was found in desminopathies. Paper-12694904. PBMC that were activated by the H. pylori hsp60 did not respond to the autologous human p60 heat shock protein. Paper-8011340. The recombinant baculoviral RT obtained with the pAc373 expression vector was purified as a p66/ p60 heterodimer. Paper-489674. In profiles corresponding to adhered pig platelets, p85 and p62 were absent, and p115 appeared highly phosphorylated. Paper-10176180. p53, c-myc p62 and proliferating cell nuclear antigen ( PCNA) expression in non-Hodgkin's lymphomas. Paper-114981. In reticulocyte lysate, the C90 fusion protein recognized the TPR proteins p60, FKBP52, and Cyp40. Paper-1508427. p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells. Paper-9881197. This increased macroautophagic flux is regulated by BAG3 in concert with the ubiquitin-binding protein p62/ SQSTM1. Paper-13707784. CVX is able to activate platelets and induce their aggregation by acting via p62/ GPVI collagen receptor. Paper-11998231. Here we show that PDEF stimulates the p62 promoter through at least two sites, and likely acts as a coactivator. Paper-9881197. All three subunits of human CAF-1 ( p150, p60, and p48) are present during the entire cell cycle. Paper-1472889. Furthermore, we demonstrate that hAsf1 proteins can interact directly with the p60 subunit of hCAF-1. Paper-9160185. Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent. Paper-1898054. In newly born cells early in G1, p34 was unphosphorylated, not associated with p62, and inactive as a protein kinase. Paper-6127019. The N-terminal region was found to be important for interaction between LC3 and p62/ SQSTM1 (hereafter termed p62). Paper-12963937. Sequestosome 1: Mutation Frequencies, Haplotypes, and Phenotypes in Familial Paget's Disease of Bone. Paper-12415738. Genomic structure and promoter analysis of the p62 gene encoding a non-proteasomal multiubiquitin chain binding protein. Paper-1588277. In this study, we report that IFN-gamma plus TLR signaling stimulates late expression of p62 in murine macrophages. Paper-13607154. The p62 scaffold regulates nerve growth factor- induced NF-kappaB activation by influencing TRAF6 polyubiquitination. Paper-10974223. We showed using tandem mass spectrometry that p60 is identical to interleukin-1 receptor-associated kinase (IRAK)-4. Paper-13418962. Deprenyl increased the expression of HO-1, PrxI, TrxI, TrxRxI, gammaGCS, and p62/ A170 in SH-SY5Y cells. Paper-10816317. Additionally, p62/ sequestosome 1 has been identified as a common component of neuropathological and hepatocytic inclusions. Paper-10044794. Ajuba recruits TRAF6 to p62 and in vitro activates PKCzeta activity and is a substrate of PKCzeta. Paper-10764634. GM-CSF also stimulated slightly but consistently tyrosine phosphorylation of GAP and p190 but not p62. Paper-48278. In vitro production of Mallory bodies and intracellular hyaline bodies: the central role of sequestosome 1/ p62. Paper-12500758. The Cul3/ Klhdc5 E3 Ligase Regulates p60/Katanin and Is Required for Normal Mitosis in Mammalian Cells. Paper-13723453. Transcript levels for the zinc transporter genes ZnT1 and Zip3 were increased and decreased, respectively, by zinc supplementation. Paper-10818213. In addition, strong p62 immunoreactivity colocalizing with myotilin aggregates was observed in myotilinopathies. Paper-12694904. In addition, LC3 directly interacts with p62/ SQSTM1 (hereafter named p62), a common constituent of protein aggregates. Paper-13065197. In response to insulin, most of the tyrosine-phosphorylated p62 was complexed to p85 alone rather than with the PIK heterodimer. Paper-119505. In interphase, p150 and p60 are bound to the nucleus, but they predominantly dissociate from chromatin during mitosis. Paper-1472889. The hypoxic degradation of p62 was blocked by autophagy inhibitors as well as by the attenuation of Atg8/ LC3 expression. Paper-13584935. Reduction of chromatin assembly factor 1 p60 and C21orf2 protein, encoded on chromosome 21, in Down syndrome brain. Paper-10138962. Together, these results demonstrate that p62 plays roles not only as an anchor but also as a regulator for the p38 kinase activity. Paper-2167695. p62 protects SH-SY5Y neuroblastoma cells against H2O2- induced injury through the PDK1/Akt pathway. Paper-13552059. Fewer subjects with PBC have autoantibodies giving nuclear rim labeling that recognize nucleoporin p62 and LBR. Paper-12572537. The binding of endogenous p62 to TRAF6 is stimulus dependent, reinforcing the notion that this is a physiologically relevant interaction. Paper-2198110. Attenuation of p62 in normoxia activated and forced expression of p62 in hypoxia blocked the activation of ERK-1/2. Paper-13584935. These MAbs specifically recognized Nup62/ p62 as evidenced by immunoblotting analysis using a nuclear membrane fraction. Paper-12008048. Abnormal accumulation of p60 in nucleus of Ndel1 null mutants supports an essential role of NDEL1 in p60 regulation. Paper-11526903. An indirect immunofluorescence study indicates that p62 colocalizes with p38 in the nucleus in response to the stimulation. Paper-2167695. Structure of the ubiquitin-associated domain of p62 ( SQSTM1) and implications for mutations that cause Paget's disease of bone. Paper-9988969. Consistent with these findings, a transgenic AD mouse model also exhibited increased p62 promoter damage and reduced p62 levels in brain. Paper-13596763. Other jasmonate-regulated (secondary) metabolic pathways might also be controlled by ORCA-like AP2/ERF-domain transcription factors. Paper-8760325. Nucleotide sequence of the protamine P1 gene from the whale Orcinus orca predicts a unique N-terminal amino-acid motif. Paper-7244716. Both p60 and FKBP52 bound specifically to the C90 domain fused to glutathione S-transferase and competed with each other for binding. Paper-1508427. Using X-ray crystallography and NMR, we herein report the structural basis for Atg8- Atg19 and LC3- p62 interactions. Paper-13503442. CDV infection induced nuclear translocation of p65 and led to a dramatic increase in sequestosome 1/ p62 and ubiquitin expression. Paper-12282872. This indicates that the endocytic uptake of AGE proteins by SMCs is mediated by an AGE receptor distinct from MSR, RAGE, p60, and p90. Paper-903699. Activation of human CDC2 protein as a histone H1 kinase is associated with complex formation with the p62 subunit. Paper-6476664. DNA ploidy and expression of the c-myc oncoprotein p62 and the v-H-ras oncoprotein p21 were examined in 54 colorectal carcinomas. Paper-86753. The absence of the p62 gene in mouse brain leads to biochemical and cognitive deficits that resemble Alzheimer disease (AD). Paper-13596763. Improvement of photoaffinity SPR imaging platform and determination of the binding site of p62/ SQSTM1 to p38 MAP kinase. Paper-13010644. However, it has not been shown how the interaction of Nup62/ p62 with transport factors is involved in nucleocytoplasmic transport. Paper-12008048. Role of p85 subunit of phosphatidylinositol-3-kinase as an adaptor molecule linking the insulin receptor, p62, and GTPase-activating protein. Paper-119505. The p60 and p64 species appear to represent phosphorylated versions on serine and threonine residues of p58 and p62. Paper-6130009. The p80 WD40 domain does not participate in p60 dimerization, but localizes to centrosomes in transfected mammalian cells. Paper-1413297. We present the first characterisation at the molecular, cellular and functional level of a non- UBA domain missense mutation (A381V) of SQSTM1. Paper-13519292. Small amounts of GTPase-activating protein (GAP) were detected in anti-Csk immunoprecipitates, suggesting that p60 may be a GAP-associated protein. Paper-536441. The ATP7B/ p62 interaction required copper, the metal-binding CXXC motifs, and the region between MBS 4 and MBS 6 of ATP7B. Paper-12004925. We have found that both of these cDNA clones encode p62, first identified as a phosphorylation independent p56(lck) SH2 domain binding protein. Paper-2167695. These results suggest that CDC2 protein may be activated as an M-phase-specific protein kinase in part by its association with the p62 subunit. Paper-6476664. Later analyses revealed the presence of fibrillarin, nucleoporin p62, and La in BIG1 and nucleolin immunoprecipitates. Paper-12769214. Increased 8-OHdG staining, a marker of oxidative stress, was observed in brain sections from mice deficient in the p62 gene compared to control. Paper-13596763. Based on these observations, we examined the interactions between Cul3, Ctb9/ KLHDC5, and the microtubule-severing protein, p60/katanin. Paper-13723453. Ubiquitin-associated domain mutations of SQSTM1 in Paget's disease of bone: evidence for a founder effect in patients of British descent. Paper-11237867. Indirect immunofluorescence showed that, like p62, p90 localized to the cytoplasm in cultured cells and mouse fetal, but not adult liver. Paper-9216145. Only a fraction of total p48 is associated with p150 and p60, and the majority is present in other high molecular weight complexes. Paper-1472889. Mutations affecting the UBA (ubiquitin-associated) domain of SQSTM1 ( Sequestosome 1) ( p62) are a common cause of Paget's disease of bone. Paper-10639923. The VP16- and p53- binding domains in these factors lie in the p62 subunit of TFIIH and in the homologous subunit, TFB1, of factor b. Paper-132970. Proteomic analysis revealed that MBs consist of ubiquitinated keratins and the stress proteins Hsp70, Hsp25, and p62. Paper-10770603. Here we demonstrate that ATM is a highly selective inhibitor of PB1-PB1 domain interactions between PKCiota and the two adaptors Par6 and p62. Paper-12232129. p62/ SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. Paper-11504287. Furthermore, the amount of the p60 and p150 subunits of CAF-1 associated with chromatin was a function of the dose of UV irradiation. Paper-1643166. The expression of six selected genes ( SQSTM1, SSRP1, DDIT4, ENAH, MAF, and PDK4) was investigated with SYBRGreen RT-Real time PCR. Paper-13756708. Treatment of MEF cells deficient in p62 with H(2)O(2) resulted in decreased cell survival and an absence of Nrf2 nuclear translocation. Paper-13596763. p62/ SQSTM1 binds directly to Atg8/ LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. Paper-13385268. We confirm the data on the existence of both a mutational hotspot at the UBA domain of SQSTM1/ p62 and a founder effect in the PDB population. Paper-13565939. We hypothesised that p62 overexpression increased EGF family receptor expression and worse outcome in breast cancer would be associated. Paper-12479354. Our findings reveal that p62 is the first protein identified that interacts with a region of the GluR receptor other than the C-terminal tail. Paper-13698282. In addition to p62, we found that Ajuba also interacted with tumor necrosis factor receptor- associated factor 6 ( TRAF6) and PKCzeta. Paper-10764634. A significant positive correlation was established between p53 LI and c-myc p62 LI (rs = 0.453) as well as between p53 LI and PCNA LI (rs = 0.338). Paper-114981. Significant correlations between immunoreactivity for TDP-43 and p62 were observed, particularly in p-FTLD-MND (Pearson correlation coefficient, 0.976). Paper-12884493. Immune complexes containing p60 and cdc2 display a cell cycle-dependent histone H1 kinase activity that is most active in interphase. Paper-6386542. Knockdown of LC3 isoforms and overexpression of LC3 mutants that fail to interact with p62 blocked the incorporation of p62 into autophagosomes. Paper-12963937. An Aspergillus fumigatus cytosolic fraction complex (CFC) composed of four proteins ( p90, p60, p40, and p37) has been identified. Paper-644661. Our data demonstrate that two independent mutational events at the same position in SQSTM1/ p62 caused PDB in a high proportion of French Canadian patients. Paper-9161805. Recurrent mutations in the ubiquitin-associated ( UBA) domain of sequestosome 1 ( SQSTM1/ p62) are identified in patients with Paget's disease. Paper-10639469. Previous work demonstrated an essential role for the atypical protein kinase C interacting protein, p62, in neurotrophin survival and differentiation signaling. Paper-9865334. A fraction of the most highly phosphorylated form of p34 was also associated with p62, a newly identified protein that became phosphorylated in vitro. Paper-6127019. The phosphorylation state of p34, its association with p62, and the protein kinase activity of the complex were each subject to cell cycle regulation. Paper-6127019. GluR1 receptor intracellular loop L2-3 and the ZZ-type zinc finger domain of p62 are essential for the interaction between these two proteins. Paper-13698282. These heterocomplexes are formed by a multiprotein chaperone machinery consisting of at least four ubiquitous proteins--hsp90, hsp70, p60 and p23. Paper-1354584. These anti-pea antibodies were specifically cross-reactive with the pea nuclear p65, p60, and p54 proteins and also with chicken lamins. Paper-10275086. Differential effects of B cell receptor and B cell receptor-FcgammaRIIB1 engagement on docking of Csk to GTPase-activating protein (GAP)-associated p62. Paper-1097212. Although NBR1 and p62 interact and form oligomers, they can function independently, as shown by autophagosomal clearance of NBR1 in p62-deficient cells. Paper-13642325. Solid phase in vitro binding assays demonstrated specific interaction of p60 with integrin alpha 3 and alpha 5 subunits but not with the beta 1 subunit. Paper-6913040. The cytoplasmic domain of the p80 TNF receptor associates with a protein kinase, termed p80TRAK, that phosphorylates both the p60 and p80 TNF receptors. Paper-984030. These SPR analysis data and empirical biologic data reveal that the binding site of p62 to p38 is the domain corresponding to 173-182. Paper-13010644. All of the mutations identified to date affect the ubiquitin-associated ( UBA) domain of SQSTM1, a region of the protein that binds noncovalently to ubiquitin. Paper-10623672. To investigate the physiological role of CAF-1 in vivo, we used RNA interference (RNAi) to silence the 60-kDa subunit of CAF-1 ( p60) in human cells. Paper-10407028. The complete amino acid sequence of the major component myoglobin from killer whale, Orcinus orca, was determined by automated Edman degradation. Paper-3905505. Antisera against the tsetse p60 protein also recognizes p63 protein of B. aphidicola, suggesting that the abundant tsetse endosymbiont protein is a chaperonin. Paper-228437. Thus far, all disease-causing mutations in SQSTM1 affect the ubiquitin-associated ( UBA) domain of the gene product and cause loss of ubiquitin binding. Paper-12415737. Novel UBA domain mutations of SQSTM1 in Paget's disease of bone: genotype phenotype correlation, functional analysis, and structural consequences. Paper-10457260. And in vitro kinase assays using MBP, but not ATF-2, as a substrate show that p62 enhances p38 activities in a dose-dependent manner. Paper-2167695. Consistent with this we show that p62 selectively interacts with the TRAF domain of TRAF6 but not that of TRAF5 or TRAF2 in co-transfection experiments. Paper-2198110. We show that p62 interacts with IFN regulatory factor 8 and Ro52, the transcription factor and ubiquitin E3 ligase that are important for IL-12p40 expression. Paper-13607154. The lack of cleavage of p60 is in contrast to the properties of p30 precursors detected in cells containing replicating avian or mammalian RNA tumor viruses. Paper-2861556. TrkA interacts with p75 receptor through the p62- TRAF-6 (tumour-necrosis-factor-receptor-associated factor 6) complex bridging the two receptors. Paper-12280281. Two out of 20 (10%) PBC sera precipitated recombinant gp210 glycoprotein and 11 out of 20 (55%) PBC sera reacted with p62 nucleoporin. Paper-13422010. Mutations in the Sequestosome 1 gene ( SQSTM1; also known as p62) have recently been identified as the cause of 5q35-linked Paget's disease of bone (PDB). Paper-10623672. Our work revealed that liganding of IL-1RI causes its strong and stable association with IL-1RAcP, MyD88, and the previously unidentified protein p60 ( IRAK-4). Paper-13418962. There was no correlation between DNA ploidy and expression of the p62 oncoprotein, and DNA ploidy did not correlate with expression of the p21 oncoprotein. Paper-86753. These octadecanoid-derivative responsive Catharanthus AP2-domain ( ORCA) proteins bind in a sequence-specific manner the JA- and elicitor-responsive element. Paper-1986150. Recently, we have reconstituted a receptor.hsp90 heterocomplex assembly system with purified rabbit hsp90 and hsp70 and bacterially expressed human p23 and p60. Paper-1380754. These include TNFRSF11A, which encodes RANK, TNFRSF11B which encodes osteoprotegerin, VCP which encodes p97, and SQSTM1 which encodes p62. Paper-13040590. Familial Paget's disease of bone has been shown to be associated with mutations in the ubiquitin-associated ( UBA) domain of the sequestosome 1 ( SQSTM1) gene. Paper-12415739. Based on these results, p60 is a plasma C3b- binding protein that shares epitopes with C4bp and MCP, and is probably not a soluble form of MCP. Paper-6322. Overexpression of p62 led to the dissociation of PDK1 from the 14-3-3theta protein, which is thought to be a negative regulator of PDK1 kinase activity. Paper-13552059. No correlation between different p62 patterns or extent of p62 expression, histological subtypes or proliferation marker Ki-67 was noted. Paper-11775588. Ubiquitin was found in a strong and constant association with keratin aggregates, whereas binding of p62 to keratin was variable. Paper-10770603. Identification and Molecular Characterization of a Novel Splice-Site Mutation (G1205C) in the SQSTM1 Gene Causing Paget's Disease of Bone in an Extended American Family. Paper-12337723. Here, we report that p62 functions to facilitate K63-polyubiquitination of TRAF6 and thereby mediates nerve growth factor-induced activation of the NF-kappaB pathway. Paper-10974223. Herein, we describe the improvement of the photoaffinity-thiol linker of our SPR imaging platform, which enabled us to determine the binding site of p62 to p38. Paper-13010644. The presence of Paget's disease in offspring who had inherited an SQSTM1 mutation was determined by bone scintigraphy and measurement of serum alkaline phosphatase ( ALP). Paper-13120964. ATM has no appreciable inhibitory effect on other PB1-PB1 domain interactions, including p62- p62, p62- NBR1, and MEKK3-MEK5 interactions. Paper-12232129. In an asynchronous cell population only a subfraction of the two large CAF-1 subunits, p150 and p60, were found to exist in a chromatin-associated fraction. Paper-1643166. Moreover, little or no murine p30 is detected in the m1MSV-transformed cells, suggesting that the murine group p30 antigenic reactivity of S + L- cells is ude to p60. Paper-2861556. Mutations affecting the ubiquitin-associated ( UBA) domain of sequestosome 1 ( SQSTM1) have recently been shown to be an important cause of PDB. Paper-11237867. Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration. Paper-11526903. Complete loss of NDEL1 or expression of dominant negative mutants of p60 in migrating neurons results in defective migration and elongation of nuclear-centrosomal distance. Paper-11526903. There was, however, a close correlation between expression of the p62 oncoprotein and that of the p21 oncoprotein being P < 0.01 according to Peason's chi-square test. Paper-86753. Altogether, these findings reveal Lys(63)-linked polyubiquitin chains and the shuttling protein p62 co-ordinately regulate TrkA internalization, trafficking and sorting. Paper-12280281. Likewise, deletion of p62 N-terminal dimerization domain or the TRAF6 binding site had similar effects on both polyubiquitination and oligomerization of TRAF6. Paper-10974223. The resulting pY-depleted DYRK1A could not regain pY during autophosphorylation but was as active as the untreated control. Paper-13296268. Alterations in the polypyrimidine tract that reduce the binding of p62 yield a corresponding reduction in the efficiency of formation of a U2 snRNP/pre-mRNA complex and splicing. Paper-5270. The AGE-receptor complex, originally described as p60 and p90, has been characterised in hemopoietic cells and the component proteins identified and designated AGE-R1, -R2 and -R3. Paper-1794772. Among them, peptides p37, p62 and p45 elicited Th1 cytokine profiles in vivo, providing help for the induction of potent CTL responses. Paper-12432761. ERp57 deletion did not affect maturation of the model facultative calnexin substrates E1 and p62 (and of most cellular proteins, as shown by lack of induction of ER stress). Paper-11310046. In our recent report, we mapped the regions in LC3 involved in its binding to p62 and showed that this binding is essential for the incorporation of p62 into autophagosomes. Paper-13065197. Here we show that p60/katanin interacts with a complex consisting of Cul3 and Ctb9/ KLHDC5, which results in ubiquitin laddering of p60/katanin. Paper-13723453. Relationship between p62 and p56, two proteins of the mammalian cortical granule envelope, and hyalin, the major component of the echinoderm hyaline layer, in hamsters. Paper-2191007. Several mutations in the ubiquitin-associated ( UBA) domain of sequestosome 1 ( SQSTM1/ p62) have been identified in patients with Paget's disease. Paper-11392257. Cotransfection of p62, keratin 8, and ubiquitin was necessary to produce in vitro type 2 MBs-like aggregates consisting of randomly oriented 10- to 15-nm filaments. Paper-12500758. Together, these results indicate that p62 is an important intermediary not only in TNFalpha but also in IL-1 signaling to NF-kappaB through the specific adapters RIP and TRAF6. Paper-2198110. The specific interaction between p62 and LC3, requiring the motif we have mapped, is instrumental in mediating autophagic degradation of the p62-positive bodies. Paper-13385268. Three proteins with approximate molecular masses of 60 ( p60), 36 ( p36), and 90 kDa ( p90) became phosphorylated after treatment with IL-1. Paper-13418962. Expression of p62 has been seen in inclusions in neoplasias like hepatocellular and breast carcinomas but also in neuronal inclusions of degenerative brain disorders. Paper-11775588. Sequestosome 1/ p62 has recently been shown to interact with polyubiquitinated proteins through its UBA domain and may direct proteins to either the UPS or autophagosome. Paper-12276297. In two pairs and one triad of unrelated Italian PDB families from different Italian regions, we detected a common SQSTM1/ p62 mutation for each P392L, M404V, and G425R group. Paper-13565939. METHODS AND RESULTS: Overexpression of p62 was detected in prostate cancer cell lines and tissues by reverse transcriptase-polymerase chain reaction. Paper-10826002. White matter lesions in the brain with frontotemporal lobar degeneration with motor neuron disease: TDP-43-immunopositive inclusions co-localize with p62, but not ubiquitin. Paper-12884493. IgE anti-Borrelia burgdorferi components ( p18, p31, p34, p41, p45, p60) and increased blood CD8+CD60+ T cells in children with Lyme disease. Paper-12474239. The presence of a CpG island as well as multiple binding sites for SP-1, AP-1, NF-kappaB, and Ets-1 family in the promoter region supports the regulated activation of the p62 gene. Paper-1588277. The mouse p62 promoter exhibited elevated oxidative damage with increasing age, and the degree of p62 promoter damage was also age-correlated in human brain samples. Paper-13596763. We originally described a methionine-->valine substitution at codon 404 (M404V) of exon 8, in the ubiquitin protein-binding domain of p62/ SQSTM1 gene in an Italian PDB patient. Paper-11472953. The gene for Sequestosome 1 ( p62; SQSTM1) has been identified as the causative gene for PDB3 in numerous French-Canadian families and families predominantly of British descent. Paper-10191233. These results indicate katanin's activities are segregated into a subunit ( p60) that possesses enzymatic activity and a subunit ( p80) that targets the enzyme to the centrosome. Paper-1413297. Studies with antibodies to GTPase activating protein (alpha-GAP) and p62 GAP-associated protein suggested that p62 was the same as (or closely related to) p62 GAP-associated protein. Paper-119505. In human subjects, the expression of p62 was decreased in AD brain relative to age-matched controls, and likewise decreased p62 expression correlated with oxidative damage to the promoter. Paper-13596763. Immunofluorescence studies with tumor-bearer sera showed only a very weak cytoplasmic fluorescence, possibly due to the nature of the p60 SH-groups in situ being masked. Paper-6087307. Screening an updated sample from Quebec and using previously published data from other populations, we compared frequency estimates for SQSTM1/ p62 mutations and haplotype distribution. Paper-12415738. Several other mutations of the SQSTM1 gene have been described in PDB patients, affecting the ubiquitin-associated domain ( UBA) of the SQSTM1/ p62 protein. Paper-12428458. It is certainly possible that the presence or absence of these ubiquitinated p62-positive yet TDP-43-negative cerebellar inclusions may act as a useful correlative factor in the future. Paper-13533302. It was also associated with the proliferation state as expressed by PCNA LI and c-myc p62 expression, indicating that the expression of these three cell cycle-related genes might be interrelated. Paper-114981. Reduction of p62 protein levels or interference with p62 function significantly increased cell death that was induced by the expression of mutant huntingtin. Paper-11504287. Northern blot analysis indicated that the p65 EcR protein is encoded by EcRB1 transcript and that the p60 and p64 USP bands were the products of USP-1 transcript. Paper-9830339. It bound to several of the repeat-containing nucleoporins ( Nup358, Nup214, Nup153, Nup98, and p62) in overlay and solution-binding assays and was competed away by karyopherin beta1. Paper-997122. Nup62/ p62, a major component of the NPC, has been reported to interact directly with several nuclear transport factors, including importin-beta and NTF2. Paper-12008048. We report the crystal structure of an ASF1a- HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1. Paper-12256737. Sporadic hyperphosphatasia syndrome featuring periostitis and accelerated skeletal turnover without receptor activator of nuclear factor-kappaB, osteoprotegerin, or sequestosome-1 gene defects. Paper-13223341. A deletion mutant of hsp90 lacking the C90 domain was unable to bind p60, whereas deletion of the approximately 25-kDa N-terminal domain of hsp90 did not affect complex formation. Paper-1508427. The gene encoding the p60 subunit of chromatin assembly factor I ( CAF1P60) maps to human chromosome 21q22.2, a region associated with some of the major features of Down syndrome. Paper-660700. The LIM protein Ajuba influences interleukin-1- induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/ p62/ TRAF6 signaling complex. Paper-10764634. Incubation with the thiol antioxidant N-acetylcysteine strongly inhibited both the Nrf2 accumulation and the expression of Nrf2- regulated genes such as HO-1, GCLM, and SQSTM1. Paper-13095938. Ubiquitin-associated ( UBA) domain mutations of SQSTM1 are an important cause of Paget's disease of bone (PDB), which is a human skeletal disorder characterized by abnormal bone turnover. Paper-12105684. Although the dose-response of p60 phosphorylation mirrors that of IRS-1, the time course is slightly slower, with maximal phosphorylation observed 5 min after addition of insulin. Paper-246040. Pull-down assays showed the non- UBA region of SQSTM1 which contains A381 is important in mediating ubiquitin-binding affinity, and that the A381V mutation exerts weak negative effects on ubiquitin-binding. Paper-13519292. MATERIALS AND METHODS: Blood was obtained from 94 unrelated French PDB patients and 100 controls for mutation screening of exons 7 and 8, encoding for the UBA domain of SQSTM1. Paper-12428458. NBR1 was localized to Ub-positive inclusions in patients with liver dysfunction, and depletion of NBR1 abolished the formation of Ub-positive p62 bodies upon puromycin treatment of cells. Paper-13642325. We conclude that the G1181 allele of TNFRSF11B, encoding lysine at codon 3 of the OPG protein, predisposes to the development of sporadic PDB and familial PDB that is not caused by SQSTM1 mutations. Paper-10653848. We demonstrate that phosphorylation of NDEL1 by Cdk5 facilitates interaction between NDEL1 and p60, suggesting that P- NDEL1 regulates the distribution of katanin p60. Paper-11526903. Under the optimum conditions, phosphoserine ( P-Ser), phosphothreonine (P-Thr) and phosphotyrosine ( P-Tyr) were satisfactorily separated in 8 min. Paper-12486377. The present study reports on the preparation of monoclonal antibodies (MAbs) directed against human Nup62/ p62 and a functional analysis of Nup62/ p62 using antibodies in living cells. Paper-12008048. In this study, we examined the extent of motor neuron disease-related changes in the brain of 9 subjects using ubiquitin-binding protein p62/ sequestosome 1 ( p62) immunohistochemistry. Paper-13495573. In mitosis, the p60 subunit of inactive CAF-1 is hyperphosphorylated, whereas active CAF-1 in interphase contains hypophosphorylated and/or phosphorylated forms of p60. Paper-1472889. To further understanding the molecular mechanism of LIS1 and lissencephaly, we identified the katanin p60 microtubule-severing protein as an additional molecular target of NDEL1. Paper-11526903. Also, recent data suggest that PB1 domains may serve to orchestrate signaling cascades not involving other PB1 domains, such as the MEK5- ERK5 and p62- ERK1 interactions. Paper-12208769. Neurons treated with supernatants from SIV-infected microglia develop a decrease in autophagy-inducing proteins, a decrease in neuronal autophagy vesicles, and an increase in sequestosome-1/ p62. Paper-13003484. The pathological features were typical for FTLD-U but with additional significant alpha-synuclein pathology, and unusual ubiquitin-positive, p62-positive, TDP-43-negative inclusions in the cerebellum. Paper-13533302. AIMS--To investigate the immunohistochemical expression of p53 protein in non-Hodgkin's lymphomas (NHL) and its relation to that of c-myc p62 oncoprotein and proliferating cell nuclear antigen ( PCNA). Paper-114981. The sequestosome 1/ p62 attenuates cytokine gene expression in activated macrophages by inhibiting IFN regulatory factor 8 and TNF receptor-associated factor 6/NF-kappaB activity. Paper-13607154. Our results reveal that IRAK is recruited to the p75- NGF receptor leading to formation of a complex between IRAK, atypical protein kinase C interacting protein, p62, and TRAF6. Paper-9167162. In BCR- ABL delta 1-40 reactivated by the SH3 deletion, transphosphoryation of other cellular proteins like p62 or SHC in vivo and autophosphorylation with recruitment of GRB-2 were also recovered. Paper-518407. Also, Cul3-deficient cells or Ctb9/ KLHDC5-deficient cells show an increase in p60/katanin levels, indicating that Cul3/Ctb9/ KLHDC5 is required for efficient p60/katanin removal. Paper-13723453. Previously reported Sequestosome 1(SQSTM1)/p62 gene mutations associated with Paget's disease of bone (PDB) cluster in, or cause deletion of, the ubiquitin-associated (UBA) domain. Paper-13853731. Deletion of this 15-aa ankyrin-binding sequence from CD44s results in a drastic reduction of HA-mediated binding/ cell adhesion, Src p60 kinase(s) interaction and anchorage-independent growth in soft agar. Paper-1388400. It was previously demonstrated that the 60,000 dalton ( p60) precursor-like polyprotein containing murine p30 was a constituent of the feline leukemia virus pseudotype of Moloney sarcoma virus [m1MSV(FeLV)]. Paper-2861556. Although the kinase activity of DYRK2 is dispensable for its ability to mediate EDVP complex formation, it is required for the phosphorylation and subsequent degradation of its downstream substrate, katanin p60. Paper-13696682. The accumulation of p62 was accompanied by elevated levels of polyubiquitylated detergent-insoluble structures. p62, however, is not required for LC3 lipidation, autophagosome formation and targeting to lysosomes. Paper-12963937. The inefficient cleavage of intracellular p60 and the kinetics of appearance of murine p30 in extracellular m1MSV(FeLV) suggest that p60 cleavage to p30 occurs in cells shortly before virus release. Paper-2861556. The monoclonal antibody Y13 259 has been used for the ras p21, the monoclonal antibody Myc1-9E10 for the c-myc p62 and the polyclonal antibody pAb1 (from Triton Bioscience Inc.) for the c-erbB-2 p185 oncoproteins. Paper-108. Using 2D gel electrophoresis and mass spectrometry, we identified p62 as a novel MB component. p62 and cytokeratins (CKs) are major MB constituents; HSP 70, HSP 25, and ubiquitinated CKs are also present. Paper-9353948. Expression of the p62 oncoprotein, determined by immunohistochemical staining, was intensely positive in 18 samples while that of the p21 oncoprotein, also determined by immunohistochemical staining, was positive in 29. Paper-86753. Nerve growth factor treatment of PC12 cells induced TRAF6 polyubiquitination along with formation of a p62-TRAF6-IKKbeta-PKC iota signal complex, while inhibition of the p62/ TRAF6 interaction had an opposite effect. Paper-10974223. Strikingly, p62/ SQSTM1, an adaptor protein that delivers ubiquitinated proteins for autophagosomal degradation, is recruited to the MR at the time of its ubiquitination and is required for its efficient degradation. Paper-13742807. The reduction in capacity for accumulation of intracellular zinc in tumorigenic prostate epithelial cells may be caused by the decrease in the ZIP1 protein expression and the intracellular redistribution of ZIP3 in RWPE2. Paper-11774634. Our results support the proposal that LC3 is responsible for recruiting p62 into autophagosomes, a process mediated by phenylalanine 52, located within the ubiquitin core, and the N-terminal region of the protein. Paper-12963937. Our results suggest that NDEL1 is essential for mitotic cell division and neuronal migration not only via regulation of cytoplasmic dynein function but also by modulation of katanin p60 localization and function. Paper-11526903. In the absence of E1A, p60 binds to the human homologue of the Schizosaccharomyces pombe cdc2 gene product, p34, to form a complex that has kinase activity that oscillates during the cell cycle. Paper-14360. The deletion also removes the promoter and initiator methionine for the p40 form of IE2 and initiator methionine for the p60 form of the protein, and therefore, these late gene products are not produced. Paper-9385802. We revealed in a previous report that p62/ SQSTM1, known to participate in proteasomal or autophagosomal protein degradation and cytokine receptor signal transduction pathways, binds to p38 to regulate specifically. Paper-13010644. Wild-type DYRK1A with a reduced level of phosphotyrosine (pY) was prepared by treating E. coli- produced DYRK1A with two different protein tyrosine phosphatases. Paper-13296268. Mammalian cortical granules contain two polypeptides ( p62 and p56) that are incorporated into the cortical granule envelope after fertilization and function in cleavage of the zygote and the preimplantation blastomeres. Paper-2191007. We recently found that a non-proteasomal protein p62 also preferentially binds multiubiquitin chains and forms a novel cytoplasmic structure " sequestosome" which serves as a storage place for ubiquitinated proteins. Paper-1715515. Here we demonstrate that the inhibition of the aPKCs or the down-regulation of p62 severely abrogates NF-kappaB activation by IL-1 and TRAF6, suggesting that both proteins are critical intermediaries in this pathway. Paper-2198110. Total kinase activity of Csk immunoprecipitates increased upon FcepsilonRI stimulation. p60 did not react with antibodies to a number of known signaling molecules, including the recently cloned, GAP-associated protein, p62dok. Paper-1560676. Unlike the bulk protein degradation that occurs during starvation, autophagic degradation of misfolded proteins can have a degree of specificity, determined by ubiquitin modification and the interactions of p62/ SQSTM1 and HDAC6. Paper-12697725. In addition, we found increased levels and aggregation of p62, suggesting that abnormal accumulation of ubiquitinated protein inclusions may contribute to the neurodegenerative phenotype observed in MLIV patients. Paper-12888041. SPR imaging experiments using a new photoaffinity linker 2 to immobilize the peptides derived from p62 on gold substrate indicate that the domain comprising amino acids 164-190 of p62 binds to p38 directly. Paper-13010644. Frontotemporal lobar degeneration with ubiquitinated tau-negative inclusions and additional alpha-synuclein pathology but also unusual cerebellar ubiquitinated p62-positive, TDP-43-negative inclusions. Paper-13533302. Immunoblotting performed with fresh cell lysates under non-reducing conditions using tumor-bearer sera revealed a diffuse p60 double band, but under reducing conditions only one sharp p60 band was observed. Paper-6087307. We examined the immunoreactivity of ubiquitin-binding protein p62 and its association with ubiquitin (Ub), alpha-synuclein, and paired helical filament (PHF)-tau in the affected brain areas of human tauopathies and synucleinopathies. Paper-8849736. We propose that the ATP7B interaction with p62 is a key component of the copper- induced trafficking pathway that delivers ATP7B to subapical vesicles of hepatocytes for the removal of excess copper into bile. Paper-12004925. Positive titres were also found in a striped dolphin ( Stenella coeruleoalba), a bottlenose dolphin ( Tursiops truncatus), a killer whale ( Orcinus orca) and a pilot whale ( Globicephala melas). Paper-1288028. These include Sp100 and promyelocytic leukemia proteins, which generate a nuclear dot IIF pattern, and two components of the nuclear pore complex specifically associated with a perinuclear pattern (i.e., gp210 and p62). Paper-11252485. Small interfering RNA (SiRNA) against Nrf2 significantly attenuated the expression of Nrf2- regulated genes such as HO-1, SQSTM1, NQO1, glutamate-cysteine ligase modifier subunit ( GCLM), and ferritin heavy chain. Paper-13095938. The PB1-domain-containing proteins p62, aPKC, MEKK2/ MEKK3, MEK5, and Par-6 play roles in critical cell processes like osteoclastogenesis, angiogenesis, and early cardiovascular development or cell polarity. Paper-12208769. This work confirms the importance of UBA domain mutations of SQSTM1 as a cause of PDB but shows that there is no correlation between the ubiquitin- binding properties of the different mutant UBA domains and disease occurrence or extent. Paper-10457260. Such cases have pathological similarities to sporadic cases with neuronal inclusions positive for ubiquitin, the ubiquitin binding protein, p62 and the newly recognised protein TDP-43 but negative for hyperphosphorylated (HP) tau. Paper-13533302. The ubiquitin-binding protein p62/ sequestosome-1 promoted aggregation of CUL3- modified caspase-8 within p62-dependent foci, leading to full activation and processing of the enzyme and driving commitment to cell death. Paper-13777524. A prominent function of p62 is the regulation of NF-kappaB activation in response to interleukin-1 ( IL-1) and tumor necrosis factor signaling through the formation of an aPKC/ p62/ TRAF6 multiprotein signaling complex. Paper-10764634. The gene encoding sequestosome 1 ( SQSTM1/ p62) maps to within the PDB3 critical region, and recent studies have identified a proline-leucine amino acid change at codon 392 of SQSTM1 (P392L) in French-Canadian patients with PDB. Paper-9338086. These results support the idea that the mammalian cortical granule envelope proteins, p62/ p56, share a common antigenic epitope(s) with echinoderm hyalin, and that p62/ p56, like hyalin, play a role in early embryogenesis. Paper-2191007. p62 is a multi-functional protein, which induces nuclear factor-kappaB (NFkappaB) activation through multiple upstream signalling pathways, including those triggered by the epidermal growth factor ( EGF) family of receptors. Paper-12479354. To monitor the autophagic sequestration of p62- and LC3-positive bodies, we developed a novel pH-sensitive fluorescent tag consisting of a tandem fusion of the red, acid-insensitive mCherry and the acid-sensitive green fluorescent proteins. Paper-13385268. Our reports identify an important role for autophagy in the selective turnover of p62, and demonstrate that in addition to the essential role of LC3 in autophagosome formation, LC3 is also involved in sorting autophagy-specific substrate(s). Paper-13065194. Notably, autophagy factors, such as the ubiquitin adaptor p62 (Refs 4, 5) and the ubiquitin-related protein Atg8 (ref. 6), associate with the MR during abscission, suggesting that autophagy is coupled to cytokinesis. Paper-13564285. ATM sensitivity correlates positively with expression of PKC iota and Par6, but not with the PKC iota binding protein p62, or the proposed targets of ATM in rheumatoid arthritis (RA), thioredoxin reductase 1 or 2. Paper-12886365. INTRODUCTION: Mutations affecting the ubiquitin-associated ( UBA) domain of Sequestosome 1 ( SQSTM1) gene have recently been identified as a common cause of familial Paget's disease of bone (PDB), but the mechanisms responsible are unclear. Paper-10457260. These pathways are also modulated by NF-kappaB mediators, including TRAF6, aPKC, p62/ SQSTM1 and deubiquitinating enzyme CYLD that are involved in the ubiquitin-proteasome system during RANK-mediated osteoclastogenesis. Paper-13513720. It is now evident that cPAcP functions as a neutral protein tyrosine phosphatase ( PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine ( p-Tyr) residues. Paper-11009111. Using polymerase chain reaction, interleukin-6 ( IL-6) cDNA fragments from harbor seal ( Phoca vitulina), killer whale ( Orcinus orca), and Southern sea otter ( Enhydra lutris nereis) were cloned and sequenced. Paper-492955. Here we show for the first time a direct interaction between p62 and the autophagic effector proteins LC3A and -B and the related gamma-aminobutyrate receptor-associated protein and gamma-aminobutyrate receptor-associated-like proteins. Paper-13385268. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport, and rapid thermal processing of biological samples contained in glass capillaries. Paper-2140202. Pulse-chase studies in cells producing m1MSV(FeLV) show that p60 is the largest polypeptide detectable during the pulse, and that intracellular p60 is not cleaved into smaller (for example, p30) polypeptides during chase periods of up to 10 hr. Paper-2861556. We identified SQSTM1 mutations in 12 of 49 families screened (24.5%), comprising 9 families with the P392L mutation and 1 family each with the following mutations: K378X, 390X, and a novel P364S mutation in exon 7, upstream of the UBA. Paper-13853731. Moreover, the binding of 125I-AGE-BSA to SMCs was affected neither by amphoterin, a ligand for one type of the AGE receptor, named RAGE, nor by 2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole-hexanoic acid-BSA, a ligand for the other AGE receptors, p60 and p90. Paper-903699. Sequestosome 1/ p62 is a scaffolding protein with several interaction modules that include a PB1 dimerization domain, a TRAF6 ( tumor necrosis factor receptor-associated factor 6) binding site, and a ubiquitin-associating (UBA) domain. Paper-10974223. The major CRS-BPs ( p60, p66, and p72) purified from bovine liver plasma membranes were found to have identical N-terminal amino acid sequence and were assumed to represent different forms of the same gene product, which we have designated CRS-BP1. Paper-154109. As we outlined in the scheme of Fig. 1, the multicomponent receptor-hsp90 heterocomplex assembly system is being reconstituted, and the importance of individual proteins, such as hsp70, p60, and p23, in the assembly process is becoming recognized. Paper-1087755. Identification of autophagy receptors, such as p62/ SQSTM1 and NBR1, which simultaneously bind both ubiquitin and autophagy-specific ubiquitin-like modifiers, LC3/ GABARAP, has provided a molecular link between ubiquitination and autophagy. Paper-13778524. The aims of this study were to examine the prevalence of SQSTM1 mutations in Australian patients, genotype/ phenotype correlations and the functional consequences of a novel point mutation (P364S) located upstream of the UBA. Paper-13853731. After applying TDP-43 immunohistochemistry to a series of 44 cases of FTLD-U with no secondary pathology, three cases (7%) were identified with ubiquitin- and p62-positive neuronal cytoplasmic inclusions (NCI) that were negative for TDP-43. Paper-12884490. Overexpression of SQSTM1 and DPP3 in IMR-32 cells stimulated NF-E2-related factor 2 ( NRF2) nuclear translocation and led to increased levels of NAD(P)H:quinone oxidoreductase 1, a protein which is transcriptionally regulated by the ARE. Paper-12465109. Chromatin assembly factor 1 ( CAF-1), a heterotrimeric complex consisting of three subunits ( p150/ p60/ p48), is one of the replication-coupled assembly factors involved in the reconstitution of S-phase chromatin. Paper-12305676. We propose that Ajuba is a new cytosolic component of the IL-1 signaling pathway modulating IL-1- induced NF-kappaB activation by influencing the assembly and activity of the aPKC/ p62/ TRAF6 multiprotein signaling complex. Paper-10764634. Four genes with different expression levels in at least two muscles ( SQSTM1, SSRP1, DDIT4, and MAF) are assigned to transcriptional cascades related to cell death and may thus indicate pathways for further investigations on congenital splay leg in piglets. Paper-13756708. The structure of the SH2/ pY complex provides insight into the ligand specificity; the BG loop in the ligand-free trans SH2 conformer is pre-arranged for optimal contacts with the pY+3 residue of the ligand. Paper-11406595. To date, five L. monocytogenes autolysins have been characterized: p60, p45, Ami, MurA and Auto and the preliminary results of our studies show that FlaA, a flagellar protein of L. monocytogenes, also has murein-degrading activity. Paper-12140725. An external geometry optimizer (BOptimize) is described that can be used together with a number of existing quantum-chemical codes (Gaussian, Gamess-UK, Turbomole, ADF, Orca, Priroda, Spartan-PM3, Mopac) and allows flexible and general constrained optimizations. Paper-13344268. Abstract Mutations affecting the ubiquitin-associated ( UBA) domain of sequestosome 1 ( SQSTM1/ p62) are commonly found in Paget's disease of bone (PDB) and impair SQSTM1's ability to bind ubiquitin, resulting in dysregulated NFkappaB signalling. Paper-13519292. Protein levels of phosphorylated eIF2alpha, a selective translation inhibitor and UPR hallmark, activating transcription factor 4 ( ATF4) and sequestosome-1 were moderately increased, whereas the cell cycle/progression protein cyclin D1 was decreased in HL60. Paper-13598110. Expression of a GTPase-deficient mutant Rab33B (Rab33B-Q92L) induced the lipidation of LC3, which is an essential process in autophagosome formation, even under nutrient-rich conditions, and attenuated macroautophagy, as judged by the degradation of p62/ sequestosome 1. Paper-12865984. In vitro translation experiments demonstrated that both the p60 and p66 species are encoded by a 3.9-kilobase (kb) mRNA which retains intron 1, while only the p60 protein is translated from a 3.6-kb L-myc mRNA which has had intron 1 removed. Paper-5849673. These proteins are also involved in selective incorporation of specific cargo molecules into autophagosomes, in which Atg8 and LC3 interact with Atg19 and p62, receptor proteins for vacuolar enzymes and disease-related protein aggregates, respectively. Paper-13503442. Sequestosome 1 Mutations in Paget's Disease of Bone in Australia: Prevalence, Genotype/ Phenotype Correlation, and a Novel Non- UBA Domain Mutation (P364S) Associated With Increased NF-kappaB Signaling Without Loss of Ubiquitin Binding. Paper-13853731. In addition, co-transfection of vectors expressing fluorescent-protein-fused TRAF6 and non-fluorescent MyD88, IRAK1 and IRAK2 revealed an inverse correlation between increased sequestosome formation and activation of both PI 3-kinase and NF-kappaB. Paper-11391603. Stimulation of a B lymphoma cell line, A20, with intact anti-IgG antibody induced a direct, SH2-mediated association between Csk and a 62-kD phosphotyrosine-containing protein that was identified as RasGTPase-activating protein-associated p62 (GAP-A.p62). Paper-1097212. Out of n = 106 tumours, 20.3% stained positively. p62 expression correlated with grade (P = 0.010) and distant metastasis (P = 0.04) and EGF receptor ( EGFR) (P = 0.012), HER2 (P = 0.016), HER3 (P = 0.007) and HER4 (0.002) expressions. Paper-12479354. Two empirical experiments, a phase discrimination experiment [Johnson et al., Animal Sonar Processes and Performance ( Plenum, New York, 1988)] and a cylinder wall thickness discrimination tasks [Au and Pawolski, J. Comp. Physiol. A 170, 41-47 (1992)] were then simulated. Paper-12509728. Evidence that BIG1 and nucleolin, but not fibrillarin, can be present with p62 at the nuclear envelope confirms the presence of BIG1 and nucleolin in dynamic molecular complexes that change in composition while moving through nuclei. Paper-12769214. To determine which subunit of PIK ( p110 or p85) p62 associates with, we first immunoprecipitated insulin-treated cell lysates with alpha-p110 and subsequently immunoprecipitated with alpha-p85 followed by Western blotting analysis with anti-phosphotyrosine antibody (alpha-PY). Paper-119505. Mutational analysis has identified MOR1, a probable stabilizer of microtubules that is a homologue of the TOGp-XMAP215 class of high-molecular-weight microtubule-associated proteins, and a katanin p60 subunit homologue implicated in the severing of microtubules. Paper-9284938. GTF2H1, the p62 subunit of the multiprotein complex transcription factor IIH ( TFIIH), participates in both the nucleotide excision repair process and transcription control by specifically interacting with a variety of factors important in carcinogenesis. Paper-13565374. Signal specificity of multifunctional enzymes is achieved through protein-protein interactions involving specific domains on scaffold proteins. p62 (also known as sequestosome 1) is such a scaffold protein that possesses PB1 and UBA domains, and the TRAF6 binding sequence. Paper-13104723. However, infected, but not uninfected children made IgG anti-B. burgdorferi proteins p18, p31, p34, p41, p45, but not IgG anti-p60, and IgE anti-B. burgdorferi proteins p31, p34, p41, p45, p60, but not IgE anti-p18. Paper-12474239. CHO-K1, TIB73, and HeLa cells were transfected with keratin 8, keratin 18, ubiquitin, p62, and p62 lacking the ubiquitin binding domain (p62DeltaUBA) and analyzed by immunofluorescence, immunoelectron microscopy, and immunoblotting. Paper-12500758. To construct the PAC and cosmid contig map spanning the HOXA cluster on human chromosome 7, we used 9 DNA markers (D7S2243, D7S3010, HOXA1, EVX1, 750, pBH8, p60, p8.0, and HOXA11), among which the final 4 were generated in this study by shotgun cloning strategy. Paper-1920173. Recently, immunoreactivity for ubiquitin-binding protein p62 has been reported in several ubiquitin-containing intraneuronal or intraglial inclusions (e.g. neurofibrillary tangles, Pick bodies, Lewy bodies, glial cytoplasmic inclusions) in various neurodegenerative diseases. Paper-10227428. Furthermore, structural analysis reveals an interaction of Trp-340 and Leu-343 of p62 with different hydrophobic pockets in the ubiquitin-fold of LC3. p62 mutants, defective in binding the LRS, escape efficient turnover by autophagy, forming ubiquitin- and p62-positive inclusions. Paper-13065194. We also show that upon macrophage stimulation, p62 binds to TNFR-associated factor 6, another E3 ligase important for NF-kappaB activation, but later this interaction was replaced by the recruitment of the deubiquitinating enzyme, cylindromatosis, an inhibitor of NF-kappaB activity. Paper-13607154. A381V mutant SQSTM1 produced a level of activation of NFkappaB signalling greater than wild type and similar to that of UBA domain mutants, indicating that non- UBA and UBA domain mutations may exert their effects via a common mechanism involving dysregulated NFkappaB signalling. Paper-13519292. Nbr1 targets the ubiquitin- associated p62/ SQSTM1 to sarcomeres, and p62 in turn interacts with MuRF2, a muscle-specific RING-B-box E3 ligase and ligand of the transactivation domain of the serum response transcription factor ( SRF). Paper-10795331. The mRNA level of p62 was increased in the brain in SIV encephalitis and as well as in brains from individuals with HIV dementia, and abnormal neuronal p62 dot structures immunoreactivity was present and had a similar pattern with abnormal ubiquitinylated proteins. Paper-12963055. MGF induced tyrosine phosphorylation of a complex of GTPase-activating protein (GAP, 120 kD) and GAP associated proteins ( p62/ p190) as detected by anti-GAP Ab immunoprecipitation followed by immunoblotting with anti-phosphotyrosine mAb. Paper-48278. Hypoxia activates mitophagy as well as macroautophagy that regulates carcinoma cell survival. p62/ SQSTM1, a multifunctional protein that targets proteins to degradation by proteasomes and autophagy, is itself downregulated by hypoxia-activated autophagy in carcinoma cells. Paper-13676619. Loss of COPI causes defects in early endosome function, as both transferrin recycling and EGF internalization and degradation are impaired, and this loss of function causes an inhibition of autophagy, an accumulation of p62/ SQSTM-1, and ubiquitinated proteins in autophagosomes. Paper-13726568. Despite the fact that calreticulin is thought to be localized in the endoplasmic reticulum, a pool of Ro/ SS A antigen homologous 60-kDa polypeptide was found to be present in the soluble cytoplasm, indicating the feasibility of an interaction of p60 with the integrin alpha subunits. Paper-6913040. To investigate whether clusterin has a function in the stress response to misfolded keratins, we performed transfection studies utilizing expression constructs encoding ubiquitin, p62, Hsp27, clusterin, keratin 8, and keratin 18. Paper-10770603. Novel polyubiquitin binders AWP1, CALCOCO2, N4BP1, RIO3, TEX27, TTC3, UBFD1 and ZNF313 were identified using this approach, while known NF-kappaB regulators including NEMO, A20, ABIN-1, ABIN-2, optineurin and p62 were also identified. Paper-13774140. These neurons display increased amounts of several autophagy-related molecules such as the scrapie-responsive gene one ( Scrg1), LC3B-II and p62 without showing any changes in mRNA expression; in addition, autolysosomes accumulate in all neuronal compartments including axon terminals. Paper-13681425. Remarkably, Atg8 and LC3 were shown to interact with Atg19 and p62, respectively, in a quite similar manner: they recognized the side-chains of Trp and Leu in a four-amino acid motif, WXXL, in Atg19 and p62 using hydrophobic pockets conserved among Atg8 homologues. Paper-13503442. Hip regions targeted for deletion and/or truncation included the C-terminal region (which has some limited homology with Saccharomyces cerevisiae Sti1 and its vertebrate homolog p60), a glycine-glycine-methionine-proline (GGMP) tandem repeat, and a tetratricopeptide repeat ( TPR). Paper-760522. Furthermore, coexpression of p62 and wild-type PDK1, the upstream kinase of Akt, further increased Akt phosphorylation and cell viability, whereas the expression of kinase-defective PDK1 reversed the cytoprotective effects of p62 under oxidative stress. Paper-13552059. The finding of ubiquitin- and p62-positive, TDP-43-negative cytoplasmic inclusions in the hippocampus and neocortex suggests reclassification of the neuropathology of FTD-3 as a unique subtype of frontotemporal lobar degeneration with ubiquitin-positive inclusions that are TDP-43-negative. Paper-12517164. These elements share no apparent sequence motif and bind different trans-acting proteins; a member of the NF kappa B family binds to the HIV-1 enhancer, the heterodimer of Jun and Fos ( AP-1) binds to the collagenase enhancer, and the serum response factors p67 and p62 bind to fos. Paper-1843. Overexpression of p62 inhibited expression of multiple cytokines, IL-12p40, TNF-alpha, IL-1beta, IL-6, and IFN-beta, whereas p62 underexpression by small hairpin RNA markedly elevated their expression, indicating that p62 is a broad negative regulator of cytokine expression in stimulated macrophages. Paper-13607154. The high penetrance of the PDB trait in this family together with the study of the asymptomatic gene carriers will allow us to confirm the proposed genotype-phenotype correlation and to evaluate the potential use of mutational analysis of the p62/ SQSTM1 gene in the early detection of relatives at risk for PDB. Paper-11472953. We have previously reported that antioxidant response element (ARE)-regulated genes, such as heme oxygenase 1 ( HO-1), sequestosome 1 ( SQSTM1), and NAD(P)H quinone oxidoreductase 1 ( NQO1), are induced in human umbilical vein endothelial cells (HUVEC) upon exposure to laminar shear stress. Paper-13095938. In this report, we describe the characterization of hZip2, a human zinc transporter identified by its similarity to zinc transporters recently characterized in fungi and plants. hZip2 is a member of the ZIP family of eukaryotic metal ion transporters that includes two other human genes, hZIP1 and hZIP3, and genes in mice and rats. Paper-2146141. Sarcomeric myosin remained in non-striated structures, Z-disk proteins, such as alpha-actinin, were mainly found in primitive dot-like structures on actin stress fibres, M-band-associated proteins ( myomesin, obscurin, Nbr1, p62 and MURF2) remained punctate. Paper-12269230. New insights from the biology of adolescent osteosarcomas, VCP and SQSTM1 mutations now defined in patients with Paget's disease, and emerging evidence that stromal lesions are present in patients with Paget's disease are changing the way we think about the pathogenesis of PDB and the rare complication of pagetic osteosarcomas. Paper-12415741. CONCLUSION: Our studies show that in vitro development of classical type 2 MBs requires overexpression of keratin 8 (or keratin 18), ubiquitin, and p62 containing the ubiquitin binding domain, whereas IHBs result from overexpression of p62 together with ubiquitin without keratin involvement. Paper-12500758. When transfected into IMR-32 neuroblastoma cells that were depleted of transcription factor NRF2 by RNA interference, SQSTM1 and DPP3 were unable to activate the ARE or induce NAD(P)H:quinone oxidoreductase 1 expression, indicating that the ARE activation upon ectopic expression of these cDNAs is mediated by NRF2. Paper-12465109. The cdc2- cyclin B kinase complex that we have isolated, consisting of two polypeptides of p60 ( cyclin B) and p34 ( cdc2), phosphorylated both the p34 and p70 subunits of the three-subunit human single-stranded DNA-binding protein (also called RP-A), a DNA replication and repair factor. Paper-100201. Our findings support the proposal that the disease mechanism in PDB with SQSTM1 mutations involves a common loss of ubiquitin binding function of SQSTM1 and implicate a sequence extrinsic to the compact globular region of the UBA domain as a critical determinant of ubiquitin recognition by the full-length SQSTM1 protein. Paper-12105684. Since the echinoderm hyaline layer and mammalian cortical granule envelope are analogous, and since the hyaline layer protein, hyalin, functions in early echinoderm embryogenesis, this study was done to determine whether p62 and p56 and/or other components of the mammalian cortical granule envelope are related to hyalin. Paper-2191007. M-CSF induced tyrosine phosphorylation of p140-200, p110, p60, p44, and p42 frequently, and that of p95 and p55 less frequently, in primary myeloid leukemic cells, whereas M-CSF-induced phosphorylation of proteins was not detected in the normal human hematopoietic cells tested. Paper-8962892. By specifically expressing TgMTP1 in Arabidopsis thaliana shoots we show that TgMTP1, localized at the vacuolar membrane, can drive enhanced shoot accumulation of Zn by initiating a systemic Zn-deficiency response that includes increased expression of Zn transporters ( ZIP3, ZIP4, ZIP5, and ZIP9) in both shoot and root tissue. Paper-13521294. Sequence analysis of exon 1 of TNFRSF11A encoding RANK, TNFRSF11B encoding OPG, and SQSTM1 encoding sequestosome-1 searched for mutations responsible for familial expansile osteolysis, expansile skeletal hyperphosphatasia, or PDB2, juvenile Paget's disease, or Paget's disease of bone (PDB), respectively. Paper-13223341. The general role of p62 in the cellular response to misfolded proteins was substantiated by detection of p62 in other cytoplasmic inclusions, such as neurofibrillary tangles, Lewy bodies, Rosenthal fibers, intracytoplasmic hyaline bodies in hepatocellular carcinoma, and alpha1- antitrypsin aggregates. Paper-9353948. To gain insight into the structural features associated with the activated form of Itk, we have solved the NMR structure of the SH2 domain bound to a phosphotyrosine-containing peptide ( pY) and analyzed changes in trans-hydrogen bond scalar couplings ((3h)J(NC')) that result from pY binding. Paper-11406595. Concomitantly, proliferating cells lacking p60 accumulated DNA double-strand breaks and increased levels of the phosphorylated histone H2A.X. Nuclear extracts from cells lacking p60 exhibited a 10-fold reduction of nucleosome assembly activity during DNA synthesis, which was restored upon addition of recombinant p60 protein. Paper-10407028. We conclude that (1) p62 protein is overexpressed in breast cancer; (2) p62 mRNA and protein increase in response to PSI, with no change of basal promoter activity; (3) PDEF upregulates p62 promoter activity through at least two sites; and (4) PSI downregulates PDEF- induced p62 promoter activation through one of these sites. Paper-9881197. In addition to this one-way cross-reaction with C4bp, a protein with a m.w. of approximately 60,000 ( p60) was found in two of three C4bp preparations that also cross-reacted with antiserum to MCP. p60 was present in trace quantities in the C4bp preparation and was successfully isolated from plasma by C3b affinity chromatography. Paper-6322. We recently identified a ubiquitin-binding protein, p62/ A170/ SQSTM1, as a molecule involved in inclusion formation. p62 interacts with LC3 which regulates autophagosome formation, through an 11 amino acid sequence rich in acidic and hydrophobic residues, named the LC3-recognition sequence (LRS), and the LC3- p62 complex is degraded by autophagy. Paper-13065194. Human p48 can bind to histone H4 in the absence of CAF-1 p150 and p60. p48, also a known subunit of a histone deacetylase, copurifies with a chromatin assembly complex (CAC), which contains the three subunits of CAF-1 ( p150, p60, p48) and H3 and H4, and promotes DNA replication-dependent chromatin assembly. Paper-717999. These synonyms are used for gene SQSTM1 (sequestosome 1): ZIP3, Ubiquitin-binding protein p62, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, PDB3, p62B, p62, p60, OSIL, ORCA, EBIAP, EBI3-associated protein of 60 kDa, A170. These accession numbers are used for gene SQSTM1: Q9BUV7 (UNIPROT__AC), BAG53577 (NCBI_GENBANK__AC), BAG35358 (NCBI_GENBANK__AC), B2R661 (UNIPROT__AC). SQSTM1 is a homologue of SQSTM1 (sequestosome 1) from Bos taurus. SQSTM1 is a homologue of SQSTM1 (sequestosome 1) from Pan troglodytes. SQSTM1 is a homologue of SQSTM1 (sequestosome 1) from Gallus gallus. SQSTM1 is a homologue of Sqstm1 (sequestosome 1) from Mus musculus. SQSTM1 is a homologue of Sqstm1 (sequestosome 1) from Rattus norvegicus. SQSTM1 is a homologue of sqstm1 (sequestosome 1) from Danio rerio. SQSTM1 is a homologue of LOC100148880 (similar to Sequestosome 1) from Danio rerio. SQSTM1 is a homologue of AgaP_AGAP009928 (AGAP009928-PA) from Anopheles gambiae str. PEST. Important links ! iHOP - Information Hyperlinked over Proteins . Concept & Implementation by Robert Hoffmann.