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Spatially distinct molecular specificity of DAG incorporation into cellular PtdCho at the level of hCEPT1 exists in situ. Paper-10661144.
Here, we propose that in addition to previously identified CEPT, hEPT1 is involved in the biosynthesis of PE via the Kennedy pathway. Paper-13122211.
The CEPT1 enzyme displayed an apparent Km of 36 microM for CDP-choline and 4.2 mol% for di-18:1 DAG with a Vmax of 14.3 nmol min(-1) mg(-1). Paper-9620516.
The preferred diradylglycerol substrates used by CEPT1 with CDP-choline as the phosphobase donor were di-18:1 DAG, di-16:1 DAG, and 16:0/18:1 DAG. Paper-9620516.
We show through immunofluorescence that brefeldin A treatment relocalizes CPT1, but not CEPT1, implying CPT1 is found in the Golgi. Paper-9333769.
The protein kinase C inhibitor chelerythrine and the human DAG kinase inhibitor R59949 both inhibited CEPT1 activity with IC50 values of 40 microM. Paper-9620516.
Rather, time-course analyses of PtdCho and lyso-PtdCho pools showed that both arachidonate-containing DAG incorporation and turnover of PtdCho is increased in hCEPT1 overexpressors. Paper-10661144.
Two human genes, CEPT1 and CPT1, code for the total compliment of activities that directly synthesize phosphatidylcholine and phosphatidylethanolamine through the CDP-alcohol pathways. Paper-9333769.
The rise of CETP activity was significantly related with the plasma steady-state drug levels (r = 0.51, P less than 0.005), thus suggesting that probucol may directly stimulate CEPT synthesis and/or release. Paper-7135315.
In addition, a series of CEPT1 site-directed mutants was generated that allowed for the assignment of specific amino acid residues as structural requirements that directly alter either phospholipid head group or fatty acyl composition. Paper-9333769.
The hCPT1 and human choline/ethanolaminephosphotransferase ( hCEPT1) predicted amino acid sequences possessed 60% overall identity and had only one variation in the amino acid residues within the CDP-alcohol phosphotransferase catalytic motif. Paper-8536588.
CEPT1 transfers a phosphobase from either CDP-choline or CDP-ethanolamine to diacylglycerol to synthesize both phosphatidylcholine and phosphatidylethanolamine, whereas CPT1 synthesizes phosphatidylcholine exclusively. Paper-9333769.
Incubation of plasma with increasing concentrations of Cu2+ resulted first in the disappearance of alpha 2-migrating HDL, the usual carrier of CEPT; free CETP and high molecular weight apoA-I-containing particles were also generated during oxidation. Paper-6954878.
In vitro assessment of hCPT1 and hCEPT1 derived cholinephosphotransferase activities also revealed differences in diradylglycerol specificities including their capacity to synthesize platelet-activating factor and platelet-activating factor precursor. Paper-8536588.
We assessed whether in vitro molecular selectivity of DAG (diacylglycerol) incorporation between CPTs is manifest in situ, by monitoring choline-d(9) incorporation into PtdCho and lyso-PtdCho molecular species over 3 h in control cells and in CHO-K1 cells overexpressing hCEPT1. Paper-10661144.
A combination of coimmunofluorescence and subcellular fractionation experiments with various endoplasmic reticulum, Golgi, and nuclear markers confirmed that CPT1 was found in the Golgi and CEPT1 was found in both the endoplasmic reticulum and nuclear membranes. Paper-9333769.
The human choline/ ethanolamine phosphotransferase 1 ( CEPT1) gene codes for a dual-specificity enzyme that catalyzes the de novo synthesis of the two major phospholipids through the transfer of a phosphobase from CDP-choline or CDP-ethanolamine to DAG to form PC and PE. Paper-9620516.
These synonyms are used for gene CEPT1 (choline/ethanolamine phosphotransferase 1): PRO1101, MGC45223, hCEPT1, DKFZp313G0615, Choline/ethanolaminephosphotransferase 1.
These accession numbers are used for gene CEPT1: Q9P0Y8 (UNIPROT__AC), Q69YJ9 (UNIPROT__AC), CAI19367 (NCBI_GENBANK__AC), AAH49196 (NCBI_GENBANK__AC).
CEPT1 is a homologue of zgc:171762 (zgc:171762) from Danio rerio.
CEPT1 is a homologue of Y49A3A.1 (hypothetical protein) from Caenorhabditis elegans.
CEPT1 is a homologue of phosphotransferase (hypothetical protein) from Caenorhabditis elegans.
CEPT1 is a homologue of Os02g0119800 (Os02g0119800) from Oryza sativa Japonica Group.
CEPT1 is a homologue of NCU03223 (similar to sn-1,2-diacylglycerol cholinephosphotransferase) from Neurospora crassa OR74A.
CEPT1 is a homologue of MGG_03690 (hypothetical protein) from Magnaporthe grisea 70-15.
CEPT1 is a homologue of CEPT1 (choline/ethanolamine phosphotransferase 1) from Bos taurus.
CEPT1 is a homologue of CEPT1 (choline/ethanolamine phosphotransferase 1) from Pan troglodytes.
CEPT1 is a homologue of CEPT1 (choline/ethanolamine phosphotransferase 1) from Gallus gallus.
CEPT1 is a homologue of CEPT1 (choline/ethanolamine phosphotransferase 1) from Canis lupus familiaris.
CEPT1 is a homologue of Cept1 (choline/ethanolaminephosphotransferase 1) from Mus musculus.
CEPT1 is a homologue of Cept1 (choline/ethanolamine phosphotransferase 1) from Rattus norvegicus.
CEPT1 is a homologue of AAPT2 (AAPT2 (AMINOALCOHOLPHOSPHOTRANSFERASE); phosphatidyltransferase/...) from Arabidopsis thaliana.
CEPT1 is a homologue of AAPT1 (AAPT1 (AMINOALCOHOLPHOSPHOTRANSFERASE 1); phosphatidyltransferase/...) from Arabidopsis thaliana.
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Concept & Implementation by Robert Hoffmann.