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These control elements include the UCE element, H1-box and H4-box. Paper-11124325.
Recently, we demonstrated that several CLIC proteins, including CLIC4, interact with AKAP350. Paper-10186569.
CLIC5 shares 52-76% overall identity with human CLIC1, CLIC2, CLIC3, and CLIC4. Paper-8398044.
CLIC4 mediates TGF-beta1-induced fibroblast-to-myofibroblast transdifferentiation in ovarian cancer. Paper-13902984.
We cloned human cDNAs and generated antibodies specific for CLIC5, CLIC1/ NCC27, and CLIC4/ huH1/ p64H1. Paper-8398044.
Northern blot analysis showed that CLIC5 has a distinct pattern of expression compared with CLIC1 and CLIC4. Paper-8398044.
Gel overlay and reverse pull-down assays indicate that the binding of CLIC4 to dynamin I and 14-3-3zeta is direct. Paper-9136069.
Moreover, in contrast to CLIC1 and CLIC4, CLIC5 is associated with the detergent-insoluble cytoskeletal fraction of microvilli. Paper-8398044.
CLIC4 is a member of the chloride intracellular channel ( CLIC) protein family whose principal cellular functions are poorly understood. Paper-10186569.
Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin. Paper-12641764.
CLIC1 and CLIC5, but not CLIC4, were strongly and reversibly inhibited (or inactivated) by 'cytosolic' F-actin in the absence of any other protein. Paper-12641764.
Using RT-PCR, comparison with CLIC1, CLIC2, CLIC3, and CLIC5 demonstrated that CLIC4 was unique by being up-regulated by TGF-beta1 in myofibroblasts. Paper-9528150.
Chloride intracellular channel protein CLIC4 ( p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms. Paper-9136069.
Immunoblot analysis of extracts from placental tissues demonstrated that CLIC4 and CLIC5 are enriched in isolated placental microvilli, whereas CLIC1 is not. Paper-8398044.
Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts. Paper-9528150.
These findings lead to the conclusion that CLIC4 and possibly other CLIC proteins have alternate cellular functions that are distinct from their proposed roles as chloride channels. Paper-10186569.
Results from spectral screening tests indicate that most H1 and H2 cells are maximally sensitive to orange light, whereas the H3 cells hyperpolarize maximally to green and depolarize maximally to red. Paper-11889321.
In this study, we incorporated purified, recombinant mammalian CLIC1, CLIC4 and (for the first time) CLIC5 into planar lipid bilayers, and tested the hypothesis that the channels are regulated by actin. Paper-12641764.
Northern blot analysis showed that p64H1 is expressed abundantly in brain and retina, whereas the other members of this family (e.g., p64 and NCC27) are expressed only at low levels in these tissues. Paper-1804713.
Indirect immunofluorescence microscopy revealed that CLIC4 and CLIC5 are concentrated within the apical region of the trophoblast, whereas CLIC1 is distributed throughout the cytoplasm. Paper-8398044.
Brain CLIC4 binds dynamin I, alpha-tubulin, beta-actin, creatine kinase and two 14-3-3 isoforms; the interactions are confirmed in vivo by immunoprecipitation. Paper-9136069.
These studies suggest that CLIC1, CLIC4, and CLIC5 play distinct roles in chloride transport and that CLIC5 interacts with the cortical actin cytoskeleton in polarized epithelial cells. Paper-8398044.
CLIC4 recruitment is strictly dependent on Galpha(13)- mediated RhoA activation and F-actin integrity, but not on Rho kinase activity; it is constitutively induced upon enforced RhoA-GTP accumulation. Paper-14161033.
Finally, a high level of aquaporin 1 unexpectedly appeared at the apical surfaces of CLIC4-suppressed RPE cells, together with a concomitant loss of basal surface expression of monocarboxylate transporter MCT3. Paper-15335866.
There is a second set of genes, consisting of 39 genes, containing two histone H1 genes, 34 genes encoding core histone proteins (H2a, H2b, H3 and H4) and three genes expressed only in the testis. Paper-12345048.
S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho- extracellular signal-regulated kinase and chloride intracellular channel 4. Paper-14024351.
We speculate that brain CLIC4 might be involved in the dynamics of neuronal plasma membrane microdomains (micropatches) containing caveolin-like proteins and might also have other cellular roles related to membrane trafficking. Paper-9136069.
Since RanBP7 specifies a key member of nuclear transport receptors responsible for the nuclear import of histone H1 and ribosomal proteins, we investigated whether this up-regulation might be proliferation-associated. Paper-8504948.
We conclude that CLIC4 is regulated by RhoA to be targeted to the plasma membrane, where it may function not as an inducible chloride channel but rather by displaying Cys-dependent transferase activity toward a yet unknown substrate. Paper-14161033.
Reduced CLIC4 expression does not interfere with S100A4/ Mts1 internalization or its interaction with myosin heavy chain IIA, but does alter alignment of myosin heavy chain IIA and actin filaments creating the appearance of vacuoles. Paper-14024351.
Three major types were found: a distal layer ( H1) of relatively small cells which were luminosity or L-type; a second and more proximal layer of larger L-type cells ( H2); and a third and yet more proximal layer of very stellate chromatic or C-type cells ( H3). Paper-11889321.
These results suggest that ROS-initiated CLIC4 up-regulation is required for TGF-beta1-induced fibroblast-to-myofibroblast transdifferentiaton in ovarian cancer, indicating that inhibiting the CLIC4 might have therapeutic potential targeting tumour stroma. Paper-13902984.
Here, we show that cytosolic CLIC4 undergoes rapid but transient translocation to discrete domains at the plasma membrane upon stimulation of G(13)-coupled, RhoA-activating receptors, such as those for lysophosphatidic acid, thrombin, and sphingosine-1-phosphate. Paper-14161033.
Conditioned medium from ovarian cancer cells (CM) or transforming growth factor-beta1 ( TGF-beta1) increased cellular reactive oxygen species (ROS) levels in fibroblasts, which initiated up-regulation of CLIC4 expression, then resulted in myofibroblast conversion. Paper-13902984.
CLIC4 is cytoplasmic in actively proliferating keratinocytes in vitro, but the cytoplasmic CLIC4 translocates to the nucleus in keratinocytes undergoing growth arrest by differentiation, senescence or transforming growth factor beta ( TGFbeta) treatment. Paper-13349093.
In HEK-293 cells, biochemical and immunofluorescence analyses show partial co-localization of recombinant CLIC4 with caveolin and with functional caveolae, which is consistent with a dynamin- associated role for CLIC4 in caveolar endocytosis. Paper-9136069.
RESULTS: We identified two copies each of the core histone H2a, H2b and H3 genes, and three copies of the H4 gene, at separate locations on chromosomes 3, 4 and 5 within the genome of Giardia intestinalis, but no gene encoding a H1 linker histone could be recognized. Paper-13238626.
Down-regulation of CLIC4 with a generic agent or specific siRNA both significantly reduces the expression of factors related to the phenotypes and functions of myofibroblasts, such as alpha-SMA, hepatocyte growth factor ( HGF) and vascular endothelial growth factor ( VEGF), thus reversing the myofibroblast phenotype back to fibroblasts. Paper-14062426.
These synonyms are used for gene CLIC4 (chloride intracellular channel 4): P64H1, p64H1, MTCLIC, Intracellular chloride ion channel protein p64H1, huH1, H1, FLJ38640, DKFZP566G223, DKFZp566G223, CLIC4L, Chloride intracellular channel protein 4.
These accession numbers are used for gene CLIC4: Q9UQJ6 (UNIPROT__AC), Q9UFW9 (UNIPROT__AC), BQ929781 (NCBI_GENBANK__AC), AL117424 (NCBI_GENBANK__AC).
CLIC4 is a homologue of LOC100334301 (chloride intracellular channel 4-like) from Danio rerio.
CLIC4 is a homologue of CLIC4 (chloride intracellular channel 4) from Pan troglodytes.
CLIC4 is a homologue of CLIC4 (chloride intracellular channel 4) from Canis lupus familiaris.
CLIC4 is a homologue of CLIC4 (chloride intracellular channel 4) from Bos taurus.
CLIC4 is a homologue of CLIC4 (chloride intracellular channel 4) from Gallus gallus.
CLIC4 is a homologue of Clic4 (chloride intracellular channel 4 (mitochondrial)) from Mus musculus.
CLIC4 is a homologue of Clic4 (chloride intracellular channel 4) from Rattus norvegicus.
CLIC4 is a homologue of clic4 (chloride intracellular channel 4) from Danio rerio.
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