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Solution structure of the HIV gp120 C5 domain. Paper-9561826.
Hereditary C5 deficiency in man: genetic linkage studies. Paper-2726811.
Linking C5 deficiency to an exonic splicing enhancer mutation. Paper-10734840.
These data suggest that AM release of LTB4 may be C5-dependent. Paper-6828088.
It is inhibited by antibodies to C5 but not affected by antibodies to C3. Paper-4547989.
Human B and T lymphoblastoid cell lines were shown to synthesize C5. Paper-6501897.
The younger had extensive dermatitis and was found to have the C5 defect. Paper-2795850.
The human C5 gene is 79 kilobases in length and is comprised of 41 exons. Paper-43045.
Chemotaxis under agarose utilizing human serum depleted of C-5 derived peptides. Paper-3758130.
Synthesis of complement component C5 by human B and T lymphoblastoid cell lines. Paper-6501897.
The fifth component of complement ( C5) has been detected in rabbit uterine flushings. Paper-5962943.
Characterization of endotoxin-induced C5-derived chemotactic activity in aqueous humor. Paper-4547989.
13C-NMR evidence of bacteriochlorophyll a formation by the C5 pathway in Chromatium. Paper-5386276.
Southern blot analysis indicates that the C-5 gene belongs to a multigene family. Paper-6785814.
In 19 of 30 family members, levels of hemolytic C5 ranged from 13 to 64 per cent of normal. Paper-3253778.
In situ hybridization refined the assignment of C5 to chromosome 9q22-33. Paper-6016110.
In both cases the treatment involves oxidation of methionine residues in the C5 protein. Paper-7527900.
Some level of C5 synthesis was detected in all eight of the B and T cell lines examined. Paper-6501897.
The isolated C5 can be used for the detection of the C5b receptors on murine macrophages. Paper-5152523.
Hereditary C5 deficiency in man. III. Studies of hemostasis and platelet responses to zymosan. Paper-2893033.
Association of the TRAF1- C5 locus on chromosome 9 with juvenile idiopathic arthritis. Paper-12882676.
Chlorophyll is synthesized from the intact carbon skeleton of glutamate via the C5 pathway. Paper-565541.
C3/ C5 convertase is a serine protease that cleaves C3 and C5. Paper-10029782.
Thirty family members in four generations were studied for C5 defiency and 32 genetic marker systems. Paper-2726811.
As a result, homozygotes and heterozygotes have a total or a partial C5 deficiency respectively. Paper-10776372.
Expression and characterization of the C345C/NTR domains of complement components C3 and C5. Paper-10102624.
Baseline SBP was significantly higher (p = 0.037) in group PL than in groups C5 and C2. Paper-1658736.
No linkage for C5 deficiency and the A or B loci of the major histocompatibility complex could be found. Paper-3253778.
T and B lymphocyte and complement ( C5) defects had no significant effect on natural resistance. Paper-5853082.
In many organisms aminolevulinate is synthesized from glutamate in a three-step pathway ( C5 pathway). Paper-6804910.
A human C5 clone (pC5HG2) was isolated from a cDNA library constructed from Hep G2 mRNA. Paper-5936125.
We performed an operation with replacement of C4 and C5 and dorsal stabilization at the same time. Paper-9326638.
This interaction is specific and involves a single C5 binding site located on the beta-subunit of C8. Paper-5697952.
Variations in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma. Paper-10630759.
Activation of the fifth component of human complement, C5, without cleavage, by methionine oxidizing agents. Paper-54587.
Earlier it was found that oxidation of C5 by hydroxyl radicals leads to its activation without cleavage. Paper-54587.
Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9. Paper-5936125.
These results indicate that, like C6, the FIMs alone in C7 mediate reversible binding to C5. Paper-10631618.
Lytic activity of NOD sera could be reconstituted with human C5 electrofocused in polyacrylamide gel. Paper-102393.
To our knowledge, this is the first human kindred with recognized combined C5 and C4 deficiency. Paper-6609932.
The presence of the fifth component of complement ( C5) in rabbit uterine flushings in relation to reproductive state. Paper-5962943.
The parents and the other children have half-normal levels of C5, and they were heterozygotes for the double mutation. Paper-10776372.
With the use of a panel of hamster-human somatic cell hybrids, the C5 gene was mapped to human chromosome 9. Paper-5936125.
A candidate gene approach identifies the TRAF1/ C5 region as a risk factor for rheumatoid arthritis. Paper-12485987.
Complement C5 in experimental autoimmune encephalomyelitis (EAE) facilitates remyelination and prevents gliosis. Paper-10028711.
Immunoelectrophoretic analysis of hydrogen peroxide-treated serum showed that C3 and C5 proteins were activated. Paper-7530453.
Both products are C5-like in that they are capable of binding C6 and form the nucleus for the cytotoxic complex C5-9. Paper-7527900.
Sera from eight family members who were apparently heterozygous for C5 deficiency gave normal chemotactic scores. Paper-2573969.
Stereospecific elimination of hydrogen at C-10 in eicosapentaenoic acid during the conversion to leukotriene C5. Paper-4356279.
Further, oxidation of about 60% of the methionine residues of C5 by Cl-T was demonstrated by amino acid analysis. Paper-54587.
The C5 convertases appeared to follow Michaelis-Menten kinetics and exhibited similar catalytic rate constants (kcat). Paper-1500991.
Recent studies have provided evidence that C3, C3a receptor, and C5 are linked to airway hyperresponsiveness. Paper-10630759.
Physiologic anterior subluxation: case report of occurrence at C5 to C6 and C6 to C7 spinal levels. Paper-10874096.
Bacteriochlorophyll c formation via the C5 pathway of 5-aminolevulinic acid synthesis in Chloroflexus aurantiacus. Paper-7084319.
Human C5 cDNA fragments were used to identify five overlapping cosmid clones that spanned the entire C5 gene. Paper-43045.
The solution structure of the C5 peptide in 40% trifluoroethanol/aqueous buffer was determined by NMR spectroscopy. Paper-9561826.
These studies suggest that C5 may have a regulatory role in the release of chemotactic activity by alveolar macrophages. Paper-5588658.
First, we have cloned newt cDNAs encoding C3 and C5 and have generated Abs specifically recognizing these molecules. Paper-9667007.
A novel role of complement: mice deficient in the fifth component of complement ( C5) exhibit impaired liver regeneration. Paper-8716033.
C5 chemotactic fragment induces leukocyte production of tissue factor activity: a link between complement and coagulation. Paper-3287502.
Anti-trypsinized C5-absorbed human serum was substituted for unabsorbed human serum in the chemotaxis under agarose system. Paper-3758130.
(6) Complications consisted of three episodes (15%) of bradycardia in the C5 group and two of bradypnea (10%) in the same group. Paper-1658736.
These labeling patterns provide evidence for the exclusive operation of the C5 pathway in ALA biosynthesis in the bacterium. Paper-5386276.
Bidirectional charge shifts of C5 mobility were observed with the two detergent systems TX-100 + DOC and TX-100 + CTAB. Paper-5968950.
Active sites in complement components C5 and C3 identified by proximity to indels in the C3/4/5 protein family. Paper-1865012.
A method has been developed for detecting cleavage of human C5 in serum and whole blood as a consequence of complement activation. Paper-4265513.
The mean distances between the dura and the ganglion and the mean spinal nerve angles increased consistently from C-5 to T-4. Paper-8574165.
Hypochlorite and taurine chloramine (T-NCI) convert native fifth component of human complement ( C5) to an activated state. Paper-234531.
Complement components C3, C4, and C5 are members of the thioester-containing alpha-macroglobulin protein superfamily. Paper-10102624.
The C5 structural gene is present as a single copy in the mouse genome as demonstrated by Southern blot analysis. Paper-34684.
C5a is an 11,000-D fragment of the fifth component of complement ( C5) with potent anaphylatoxic and leukocyte chemotactic activities. Paper-4909636.
The presence of C5 in trout suggests that C3, C4, and C5 must have diverged before the appearance of teleost fish. Paper-8976402.
Finally, the C5 gene was found to be expressed in naive, GC, and memory B lymphocytes at both the mRNA and the protein level. Paper-1865004.
Comparison of C5 with the homologous family members C3 and C4 revealed striking similarities in exon size and number. Paper-43045.
RESULTS: We show that DCs produce C3, C5, C9, Factor (F)I, FH, FB, FD and properdin at levels similar to macrophages. Paper-12141590.
Genetic linkage studies were performed on the only reported kindred with genetic deficiency of the fifth component of complement ( C5). Paper-2726811.
We also identified a haplotype of the C5 gene that was protective against childhood BA (P = 1.4 x 10(-6)) and adult BA (P = 0.00063). Paper-10630759.
Studies reported over 30 years ago revealed that latent, nonactivated C5 binds specifically and reversibly to C6 and C7. Paper-10631618.
Analysis of interactions between purified C8 and C5, C6, or C7 indicate that C5 and C8 associate to form a dimer in solution. Paper-5697952.
The C5 alpha-chain was encoded by 49 kilobases containing 26 exons; the beta-chain was encoded by 29 kilobases containing 16 exons. Paper-34684.
In contrast, T and B lymphocyte and complement ( C5) defects had little effect on the clearance of Escherichia coli from the kidneys. Paper-4872169.
In contrast, T and B lymphocyte and complement ( C5) defects had little effect on the clearance of Escherichia coli from the kidneys. Paper-4543506.
The conversion is accompanied and probably caused by oxidation of methionine residues in the C5 protein to methionine sulfoxide. Paper-234531.
The C1 and C5 domains of gp120 are thought to directly interact with gp41 but are largely missing from the available X-ray structure. Paper-9561826.
Reconstitution of C5-deficient mice with murine C5 or C5a significantly restored hepatocyte regeneration after toxic injury. Paper-8716033.
Twenty-three synthetic peptides with C5 sequences and 10 with C3 sequences were also tested for the ability to inhibit C hemolytic activity. Paper-1865012.
Macrophages from such mice were unable to present biosynthesized C5 to class II-restricted T cells, even when preactivated in vivo. Paper-518375.
They contained taurine chloramine, estimated photometrically, and they activated C5 upon incubation, assayed functionally by reactive lysis. Paper-839510.
Recently, C5-derived peptides have been implicated as being mediators of acute lung injury (i.e., " shock lung") in some clinical situations. Paper-4302610.
In the absence of added taurine the hypochlorite formed by MPO oxidized endogenous amines that also activated C5. Paper-839510.
(4) Sedation and anxiolysis were more pronounced (p = 0.0001) in groups C5 and C2.5 than in groups D and PL and in group C5 than in group C2. Paper-1658736.
This process requires the availability of the fifth component of complement ( C5) but not the presence of other components ( C2 and C6- C9). Paper-6336480.
These C3b-containing C3/ C5 convertases exhibited Km (0.0051 microM) well below the normal concentration of C5 in blood (0.37 microM). Paper-10029782.
Combined complete C5 and partial C4 deficiency in humans: clinical consequences and complement-mediated functions in vitro. Paper-6609932.
The asymptomatic first degree family members had C5 levels compatible with a heterozygous state of C5 deficiency. Paper-6609932.
Activation of the fifth component of human complement ( C5) induced by monosodium urate crystals: C5 convertase assembly on the crystal surface. Paper-3986787.
The C5 structural gene was found to be a highly interrupted gene of approximately 78 kilobases containing 42 exons and 41 introns. Paper-34684.
The complex of activated C5 with C6 is capable of combining with the components C7, C8, and C9 forming the cytotoxic terminal complement complex C5-9. Paper-234531.
Generation of slow reacting substance by human leukocytes. II. Comparison of stimulation by antigen, anti-IgE, calcium ionophore, and C5-peptide. Paper-3647016.
The most positive lod score (1.07, theta=0.05) was for linkage between C5 and haptoglobin, but this score does not reach statistical significance. Paper-2726811.
Structural aspects of the human C5 gene. Intron/exon organization, 5'-flanking region features, and characterization of two truncated cDNA clones. Paper-43045.
Histamine suppresses in vitro synthesis of precursor (pro- C5) of the fifth complement component ( C5) by mouse peritoneal macrophages. Paper-4198438.
Soluble histamine added to mouse peritoneal macrophages in culture suppressed the synthesis of the functional and antigenic complement component C5. Paper-4198438.
When taurine was omitted from the PMN suspensions during stimulations much less oxidant was found in the supernatants and less C5 activating potency. Paper-839510.
These findings provide direct support for previous evidence suggesting the biologic activity for neutrophils derives entirely from the alpha subunit of C5. Paper-3258549.
Polymorphonuclear (PMN) leukocytes from LPS-treated rabbits exhibit diminished responsiveness in vitro to complement ( C5) -derived peptides. Paper-5127632.
The C5a molecule is one of two spasmogenic fragments (i.e. C3a and C5a) released from serum components C3 and C5 during complement activation. Paper-3050930.
Chemotactic activity for human polymorphonuclear leukocytes was generated that was inhibitable by incubation with anti- C5, but not with anti-C3 antisera. Paper-3794470.
Because one potent chemotactic factor released by AMs is leukotriene B4 ( LTB4), we hypothesized that cell surface C5 may modulate LTB4 release. Paper-6828088.
The family is the largest C5-deficient kindred to be reported and emphasizes the importance of C5 in host susceptibility to invasive Neisseria infections. Paper-3956020.
Three of the C5 peptides and one of the C3 peptides showed 50% inhibition of both C hemolytic and bactericidal activities at a concentration of 100 microM. Paper-1865012.
Stereoselective synthesis of conformationally constrained 2'-deoxy-4'-thia beta-anomeric spirocyclic nucleosides featuring either hydroxyl configuration at C5'. Paper-11232200.
Overall, the results suggest that regions important for function of both C3 and C5 lie proximal to residues 150-200 and 1600-1620 in the precursor sequences. Paper-1865012.
The reversible interactions of C5 with C6, C7, and major polypeptide fragments derived from these were investigated with solid phase binding assays. Paper-68433.
Two truncated human C5 clones, pHC5A and pHC5B, were isolated from an adult human liver cDNA library, and contained inserts of 2930 and 2181 bp, respectively. Paper-6864082.
The DNA sequence showed that the pC5HG2 insert was comprised of 3309 base pairs of pro- C5 coding sequence and 404 base pairs of 3'-untranslated sequence. Paper-5936125.
Truncated C5 mRNA were not detected in Raji, MOLT-4, human fibroblast or U937 cells, although the full-length 6.0-kb transcript was seen in MOLT-4 cells. Paper-6864082.
RNase P from Escherichia coli is a tRNA-processing enzyme and consists of a catalytic RNA subunit ( M1 RNA) and a protein component ( C5 protein). Paper-8408629.
CONCLUSIONS: A common genetic variant at the TRAF1- C5 locus on chromosome 9 is associated with an increased risk of anti-CCP-positive rheumatoid arthritis. Paper-12484865.
We have analyzed the role of complement component C5 in experimental autoimmune encephalomyelitis (EAE) using C5-deficient (C5-d) and C5-sufficient (C5-s) mice. Paper-10028711.
The results indicate that much of the random migration observed in the chemotaxis under agarose system is due to C5-derived peptides present in normal human serum. Paper-3758130.
In contrast to the recently described C5-dependent pathway in PMN receptor modulation by CU, this effect seemed to depend on the presence of the complement factor C3. Paper-8704602.
Based on the experimental results, an intercalation model that permits competitive hydrogen abstraction from C5' and C4' of deoxyribose moiety has been proposed. Paper-6843399.
These labelling patterns provide evidence for the operation of the C5 pathway of 5-aminolevulinic acid synthesis for bacteriochlorophyll c formation in the bacterium. Paper-5389364.
When added to sensitive target cells ( guinea pig or chicken erythrocytes), C5 mediated lysis by C7-C9 without the addition of C6 or any activation procedure. Paper-4520354.
In particular, mAb 1.5 was active both on fluid-phase and on membrane-bound C5 and is therefore likely to interact with the binding site for the late components on C5. Paper-23852.
The C5 domain of the collagen VI alpha3(VI) chain is critical for extracellular microfibril formation and is present in the extracellular matrix of cultured cells. Paper-12034041.
Seven of these aptamers formed a closely related sequence homology family; these aptamers bound C5 with a Kd 20-40 nM and also inhibited human serum hemolytic activity. Paper-1970778.
In this study we show that mice lacking the hepatic transcription factor hepatocyte nuclear factor 1alpha (HNF1alpha) fail to transcribe C5 and C8A complement genes. Paper-8865876.
Both differ from C5b, the natural activation product of C5, as they comprise the whole, uncleaved C5 protein, and do not immediately decay when not bound to C6. Paper-7527900.
Similarly, during the process of lens regeneration, C3 was detected in the iris and cornea, while C5 was present in the regenerating lens vesicle as well as the cornea. Paper-9667007.
Biophysical studies of the HIV gp120 C5 domain (residues 489-511 of HIV-1 strain HXB2), which corresponds to the carboxy terminal region of gp120, have been undertaken. Paper-9561826.
Very high affinity C5 convertases were generated only when the low affinity C3/ C5 convertases (EAC1,C4b,C2a) were allowed to deposit C3b by cleaving native C3. Paper-10029782.
C5 levels of other family members were either normal or approximately half-normal, consistent with autosomal codominant inheritance of the gene determining C5 deficiency. Paper-2483366.
Since C5a peptides induce selective chemotactic attraction of polymorphonuclear leukocytes (PMN), measurement of PMN-induced chemotaxis was used as an index of C5 activation. Paper-11445583.
Human polymorphonuclear leukocytes (PMN) were stimulated in vitro by incubation with opsonized zymosan; thereafter the supernatants were tested for C5 activating potency. Paper-839510.
The ASP incubation mixture with C5 but not C3 elicited vascular leakage in a dose- and incubation time-dependent manner, which was inhibited by a histamine H(1)-receptor antagonist. Paper-12913419.
Experiments with mastocytoma cells showed that a hydrogen is stereospecifically eliminated from C-10 during the conversion of eicosapentaenoic acid to leukotriene C5. Paper-4356279.
However, near the 3'-ends, at exon/ intron boundaries, the nucleotide sequences of pHC5A and pHC5B diverged from each other and from the full-length 6.0-kb C5 cDNA sequence. Paper-6864082.
We observed a significant association (p < 0.05) of SNPs located in a haplotype block that encompasses a 65 kb region including the 3' end of C5 as well as TRAF1. Paper-12485987.
The oxidation product of methionine, its sulphoxide, may cause a change in structural conformation of C5 which involves expression of the C6 binding site. Paper-54587.
CD studies of the C5 domain suggest that it is unstructured in aqueous solutions but partially helical in trifluoroethanol/aqueous and hexafluoroisopropanol/aqueous buffers. Paper-9561826.
These findings indicate that the C5 activating agent was produced by stimulated PMN through MPO-generated hypochlorite, trapped as taurine chloramine. Paper-839510.
Furthermore, both wheezing phenotypes showed decreased expression of the complement component 5 receptor 1 gene, a gene involved in the regulation of bronchial responsiveness. Paper-13066783.
Monoclonal antibodies as probes to investigate the molecular changes of C5 associated with the different stability of the molecule on sheep erythrocytes and Escherichia coli 0111:B4. Paper-23852.
The suppression of C5 activity in resident and thioglycollate-stimulated cultures was dependent on the dose of histamine with maximal suppression occurring at a concentration of 10(-3) M. Paper-4198438.
Conditions were chosen for treatment of C5 with Cl-T which exclude attack on tryptophan, and we have ensured that human C5 does not contain free cysteine residues. Paper-54587.
Replication of the tumor necrosis factor receptor-associated factor 1/ complement component 5 region as a susceptibility locus for rheumatoid arthritis in a European family-based study. Paper-12987500.
C5 was identified on guinea pig alveolar macrophages by epifluorescent microscopy, flow cytometry, and enzyme-linked immunoabsorbent assay of eluted macrophages. Paper-5588658.
Inherited human complement C5 deficiency. Nonsense mutations in exons 1 (Gln1 to Stop) and 36 (Arg1458 to Stop) and compound heterozygosity in three African-American families. Paper-214428.
A novel promoter polymorphism in the gene encoding complement component 5 receptor 1 on chromosome 19q13.3 is not associated with asthma and atopy in three independent populations. Paper-10323009.
Thus, among the genes for complement components which can be mapped because of deficiency states or polymorphic gene products, C5 joins C1r, C3 and C6 in not being closely linked to HLA. Paper-2726811.
This is evident from loss of functional properties of native C5 and acquisition of a binding site for C6 which is characteristic of C5b, the physiological activation fragment of C5. Paper-234531.
The deduced amino acid sequence of a partial cDNA clone (2.25kb), representing approximately 44% of the coding sequence, showed 60 and 58% similarity to human and mouse C5, respectively. Paper-8976402.
Examination of cytoplasmic RNA prepared from human liver and the human hepatoma cell line Hep G2 by Northern transfer has indicated a C5 mRNA species of about 5.2 kilobase pairs. Paper-5082442.
The high binding affinities suggest that complexes composed of C5 bound to C6 or C7 exist in plasma before activation and may facilitate assembly of the complement membrane attack complex. Paper-10102624.
The derived amino acid sequence contained the entire coding sequence of the C5 alpha-chain, the beta-alpha-chain junction region, and 100 amino acids (approximately 50%) of the beta-chain. Paper-5936125.
PAF-induced eosinophil degranulation was inhibited by CB, CD, or anti-CD18 antibodies, whereas complement component 5-induced degranulation was not suppressed by CB. Paper-9777662.
Sera from all family members showed normal IIP values, with exception of the subject with combined partial deficiency in C4A, C4B, and complete deficiency in C5. Paper-6609932.
It was also found that 5(-14)C-labelled glutamate and 2-oxoglutarate were incorporated into these chlorophylls which is consistent with the operation of both the C5 and Shemin pathways. Paper-4042296.
In the present study we examined the C5 cleaving properties of classical pathway C3/ C5 convertase either bound to the surface of sheep erythrocytes or in its free soluble form. Paper-10029782.
Direct sequencing of all C5 exons displayed no mutation of obvious functional significance, except for an A to G transition in exon 10 predicting an exchange from lysine to arginine. Paper-10734840.
This report describes additional site-specific mutagenesis and synthetic peptide inhibition studies aimed at testing this idea for the paralogous complement C3, C4, and C5 proteins. Paper-1865012.
The C5 cDNA clone was sheared randomly by sonication, subcloned into M13 mp8, and sequenced at random by the dideoxy technique, thereby generating a contiguous sequence of 1703 base pairs. Paper-5082442.
The serum glycoprotein C5a, which is derived from the proteolytic cleavage of complement protein C5, has been implicated in the pathogenesis of a number of inflammatory and allergic conditions. Paper-1057732.
Previously, we have stablished that the fifth component of complement ( C5) serves as an important source of mediators that have locomotory (chemotactic) activity for leukocytes and tumor cells. Paper-3334779.
Lymphoblastoid cells with both distributions of C5 were shown to synthesize a messenger RNA that was similar in size to the C5 mRNA expressed by the HepG2 hepatoma cell line. Paper-6501897.
Deposition of C3b converts the enzymes to high affinity C5 convertases, which cleave C5 in blood at catalytic rates approaching Vmax, thereby switching from C3 to C5 cleavage. Paper-10029782.
Amino acid sequence alignment of the homologous family members C3, C4, and alpha 2-macroglobulin with that of C5 demonstrated 27%, 25%, and 19% identity, respectively. Paper-5936125.
Clone pHC5B encoded the entire C5 signal peptide, the beta-chain, the linker peptide, nine amino acids of the alpha-chain, and six amino acids of divergent protein sequence in an open reading frame. Paper-6864082.
Most importantly, C5 deficiency was excluded ( lod score greater than -2.0) from linkage with the major histocompatibility locus (HLA) from a recombination frequency of greater than 15% (in females). Paper-2726811.
These results suggest that the C3 and C5 pathways of the complement system play important roles in the pathogenesis of BA and that polymorphisms of these genes affect susceptibility to BA. Paper-10630759.
The chemotactic factor, of which the apparent molecular mass was 75 kDa, shared antigenicity with complement C5 and possessed the affinity to monocytes, but not to polymorphonuclear leukocytes. Paper-7209512.
Sialic acid-independent modulation by H of C5 cleavage by the C5 convertase represents an additional regulatory step in the activation of the human alternative complement pathway. Paper-4277144.
Comparison of the derived partial human C5 protein sequence with that previously determined for murine C3 and human alpha 2-macroglobulin has indicated regions of pronounced sequence similarity. Paper-5082442.
The defect in Leiner's disease, which presents in early infancy with extensive dermatitis, diarrhoea, and failure to thrive, has been attributed to a defect of the fifth component of complement ( C5). Paper-2795850.
CVF-B purified by gel filtration could initiate the hemolysis of unsensitized guinea pig erythrocytes when incubated with human complement components C5 to C9 in 0.01 M EDTA buffer. Paper-2312473.
In addition, the C5 structure lends insight into the site of HIV envelope protein maturation by the host enzymes furin and PC7, which provides other possible targets for drug therapies. Paper-9561826.
BACKGROUND: The inflammatory functions of complement component 5 ( C5) are mediated by its receptor, C5R1, which is expressed on bronchial, epithelial, vascular endothelial and smooth muscle cells. Paper-10323009.
In three of the cell lines, C5 was detected in both culture supernatants and whole cell detergent lysates, whereas in the other five cell lines, C5 was detected only in the cell lysates. Paper-6501897.
To understand fifth complement component ( C5) gene regulation, splicing, and C5 protein deficiency at the molecular level, the organization of the murine C5 gene was determined. Paper-34684.
The 2-base-pair gene deletion and HindIII and PvuII restriction fragment length polymorphisms associated with murine C5 deficiency were localized to exon 7, exon 16, and intron 20, respectively. Paper-34684.
CONCLUSION: Our findings indicate that C5 gene variants and Gc-globulin levels co-define the proinflammatory and profibrogenic effects of C5 in patients at-risk for progression of liver fibrosis. Paper-13319650.
Moreover, challenged C5-deficient mice had a 3.4-fold and 2.7-fold increase in the levels of airway eosinophils and lung interleukin (IL)-4-producing cells, respectively, compared with challenged wild-type mice. Paper-11371702.
The Escherichia coli ribonuclease P ( RNase P) has a protein component, termed C5, which acts as a cofactor for the catalytic M1 RNA subunit that processes the 5' leader sequence of precursor tRNA. Paper-11057717.
It is intriguing to speculate, therefore, that circulating C5-derived chemotactic peptides may play a role in the pathogenesis of the lung injury observed in some patients during the course of acute pancreatitis. Paper-4302610.
Mycoplasma pulmonis inoculated parenterally into mice deficient in the fifth component of complement ( C5) caused a chronic arthritis of significantly greater magnitude than in immunologically normal mice. Paper-2991488.
To investigate the involvement of C5a in the pathophysiology of A. sobria infection, we examined release of C5a from human C5 by a serine protease (ASP), a putative virulence factor secreted by this bacterium. Paper-12913419.
Structure of the murine fifth complement component ( C5) gene. A large, highly interrupted gene with a variant donor splice site and organizational homology with the third and fourth complement component genes. Paper-34684.
The basophils of patients with asthma and control subjects responded similarly to most stimuli: they were significantly less responsive to C5-peptide and f-met peptide, and significantly more responsive to D2O. Paper-3611286.
OBJECT: The incidence of postoperative C-5 spinal nerve root palsy following decompressive cervical spinal surgery has been reported to be as high as 12% for anterior procedures and 30% for posterior procedures. Paper-13270275.
The results indicate that the carboxyl-terminal domains of C6 and C7, which have cysteine-rich modules homologous to those found in factors H and I, have the capacity to link specifically with C5. Paper-68433.
Fluorescence titration experiments suggest that HIV C5 forms a 1 : 1 complex with the HIV gp41 ectodomain in the presence of cosolvent with an apparent Kd of approximately 1.0 micro m. Paper-9561826.
CONCLUSIONS: These data indicate that C5 plays an important protective role in allergic lung disease by suppressing inflammatory responses and Th2 effector functions observed in this experimental model. Paper-11371702.
Treatment of DCs with lipopolysaccharide ( LPS) promoted an increase in the expression of C3 and FI mRNAs and a decrease in C5 mRNA, while C9, FH, FB, FD and properdin mRNA levels were not affected. Paper-12141590.
After enzymatic C3b coupling or standard amine C3b coupling, we analyzed and compared the binding of four C3b ligands to the surface: factor B, factor H, C5 and the soluble complement receptor 1 (sCR1, CD35). Paper-8955888.
Thus, the mouse quantitative trait gene led to the identification of an unknown gene underlying human susceptibility to liver fibrosis, supporting the idea that C5 has a causal role in fibrogenesis across species. Paper-10758238.
Because C5-deficient macrophages could be activated from extraneous C5 and TNF-alpha we suggest that both play a role in the macrophage-mediated killing as well as containment mechanisms in tuberculosis. Paper-8449715.
Trypsin-activated complex of human factor B with cobra venom factor (CVF), cleaving C3 and C5 and generating a lytic factor for unsensitized guinea pig erythrocytes. I. Generation of the activated complex. Paper-2312473.
Regulation by H of formation of the C3 and C5 alternative pathway convertases of complement on cells is dependent on such chemical characteristics of the cell surfaces as their membrane content in sialic acid. Paper-4277144.
The fractional catabolic rate and metabolism of factor B in vivo is similar to that of C3, C4 and C5 complement proteins, which are among the most metabolically active plasma proteins in the circulatory system. Paper-6139292.
Culturing the AMs with anti- C5 Fab' antibodies inhibited the release of LTB4 induced by opsonized zymosan, immune complexes, or lipopolysaccharide (78%, 41%, and 82% inhibition, respectively, p less than 0.05). Paper-6828088.
The C3 and C5 proteins were expressed in a complementary fashion during limb regeneration, with C3 being expressed mainly in the blastema and C5 exclusively in the wound epithelium. Paper-9667007.
The data suggest that C3/ C5 convertase assembled with either monomeric C4b or C4b-C4b complexes are inefficient in capturing C5 but cleave C3 opsonizing the cell surface with C3b for phagocytosis. Paper-10029782.
These cells were challenged with several different stimuli: goat anti-human IgE-Fc, C5-peptide, N-formyl-methionyl-leucyl-phenylalanine (f-met peptide), Ca++ ionophore A23187, hyperosmolar mannitol, and D2O. Paper-3611286.
A C5-depleted reagent (C5D) was generated from the non-adsorbed protein containing fractions obtained subsequent to the passage of freshly drawn NHS plus 10 mM EDTA through the monospecific anti- C5 Sepharose 4B column. Paper-3700429.
The SNP is in linkage disequilibrium with two genes relevant to chronic inflammation: TRAF1 ( encoding