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Solution structure of the HIV gp120 C5 domain. Paper-9561826.
Hereditary C5 deficiency in man: genetic linkage studies. Paper-2726811.
These data suggest that AM release of LTB4 may be C5-dependent. Paper-6828088.
It is inhibited by antibodies to C5 but not affected by antibodies to C3. Paper-4547989.
Human B and T lymphoblastoid cell lines were shown to synthesize C5. Paper-6501897.
The younger had extensive dermatitis and was found to have the C5 defect. Paper-2795850.
The human C5 gene is 79 kilobases in length and is comprised of 41 exons. Paper-43045.
Chemotaxis under agarose utilizing human serum depleted of C-5 derived peptides. Paper-3758130.
Synthesis of complement component C5 by human B and T lymphoblastoid cell lines. Paper-6501897.
The fifth component of complement ( C5) has been detected in rabbit uterine flushings. Paper-5962943.
Characterization of endotoxin-induced C5-derived chemotactic activity in aqueous humor. Paper-4547989.
13C-NMR evidence of bacteriochlorophyll a formation by the C5 pathway in Chromatium. Paper-5386276.
Southern blot analysis indicates that the C-5 gene belongs to a multigene family. Paper-6785814.
In 19 of 30 family members, levels of hemolytic C5 ranged from 13 to 64 per cent of normal. Paper-3253778.
In situ hybridization refined the assignment of C5 to chromosome 9q22-33. Paper-6016110.
In both cases the treatment involves oxidation of methionine residues in the C5 protein. Paper-7527900.
Some level of C5 synthesis was detected in all eight of the B and T cell lines examined. Paper-6501897.
The isolated C5 can be used for the detection of the C5b receptors on murine macrophages. Paper-5152523.
Hereditary C5 deficiency in man. III. Studies of hemostasis and platelet responses to zymosan. Paper-2893033.
Chlorophyll is synthesized from the intact carbon skeleton of glutamate via the C5 pathway. Paper-565541.
Thirty family members in four generations were studied for C5 defiency and 32 genetic marker systems. Paper-2726811.
As a result, homozygotes and heterozygotes have a total or a partial C5 deficiency respectively. Paper-10776372.
No linkage for C5 deficiency and the A or B loci of the major histocompatibility complex could be found. Paper-3253778.
T and B lymphocyte and complement ( C5) defects had no significant effect on natural resistance. Paper-5853082.
In many organisms aminolevulinate is synthesized from glutamate in a three-step pathway ( C5 pathway). Paper-6804910.
This interaction is specific and involves a single C5 binding site located on the beta-subunit of C8. Paper-5697952.
Activation of the fifth component of human complement, C5, without cleavage, by methionine oxidizing agents. Paper-54587.
Earlier it was found that oxidation of C5 by hydroxyl radicals leads to its activation without cleavage. Paper-54587.
Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9. Paper-5936125.
The presence of the fifth component of complement ( C5) in rabbit uterine flushings in relation to reproductive state. Paper-5962943.
The parents and the other children have half-normal levels of C5, and they were heterozygotes for the double mutation. Paper-10776372.
With the use of a panel of hamster-human somatic cell hybrids, the C5 gene was mapped to human chromosome 9. Paper-5936125.
Purified human C5 was incubated with chloramine T (Cl-T) or N-chloro-succinimide (N-Cl-S) in barbital buffer, pH 7. Paper-54587.
We observed the absence of component C5 in the serum of 3 siblings from a Brazilian family with history of consanguinity. Paper-13796286.
Complement C5 in experimental autoimmune encephalomyelitis (EAE) facilitates remyelination and prevents gliosis. Paper-10028711.
Both products are C5-like in that they are capable of binding C6 and form the nucleus for the cytotoxic complex C5-9. Paper-7527900.
Stereospecific elimination of hydrogen at C-10 in eicosapentaenoic acid during the conversion to leukotriene C5. Paper-4356279.
Further, oxidation of about 60% of the methionine residues of C5 by Cl-T was demonstrated by amino acid analysis. Paper-54587.
The C5 convertases appeared to follow Michaelis-Menten kinetics and exhibited similar catalytic rate constants (kcat). Paper-1500991.
Bacteriochlorophyll c formation via the C5 pathway of 5-aminolevulinic acid synthesis in Chloroflexus aurantiacus. Paper-7084319.
The solution structure of the C5 peptide in 40% trifluoroethanol/aqueous buffer was determined by NMR spectroscopy. Paper-9561826.
These studies suggest that C5 may have a regulatory role in the release of chemotactic activity by alveolar macrophages. Paper-5588658.
A novel role of complement: mice deficient in the fifth component of complement ( C5) exhibit impaired liver regeneration. Paper-8716033.
C5 chemotactic fragment induces leukocyte production of tissue factor activity: a link between complement and coagulation. Paper-3287502.
Anti-trypsinized C5-absorbed human serum was substituted for unabsorbed human serum in the chemotaxis under agarose system. Paper-3758130.
(6) Complications consisted of three episodes (15%) of bradycardia in the C5 group and two of bradypnea (10%) in the same group. Paper-1658736.
These labeling patterns provide evidence for the exclusive operation of the C5 pathway in ALA biosynthesis in the bacterium. Paper-5386276.
Bidirectional charge shifts of C5 mobility were observed with the two detergent systems TX-100 + DOC and TX-100 + CTAB. Paper-5968950.
Active sites in complement components C5 and C3 identified by proximity to indels in the C3/4/5 protein family. Paper-1865012.
A method has been developed for detecting cleavage of human C5 in serum and whole blood as a consequence of complement activation. Paper-4265513.
The mean distances between the dura and the ganglion and the mean spinal nerve angles increased consistently from C-5 to T-4. Paper-8574165.
Hypochlorite and taurine chloramine (T-NCI) convert native fifth component of human complement ( C5) to an activated state. Paper-234531.
The C5 structural gene is present as a single copy in the mouse genome as demonstrated by Southern blot analysis. Paper-34684.
C5a is an 11,000-D fragment of the fifth component of complement ( C5) with potent anaphylatoxic and leukocyte chemotactic activities. Paper-4909636.
Finally, the C5 gene was found to be expressed in naive, GC, and memory B lymphocytes at both the mRNA and the protein level. Paper-1865004.
Comparison of C5 with the homologous family members C3 and C4 revealed striking similarities in exon size and number. Paper-43045.
Genetic linkage studies were performed on the only reported kindred with genetic deficiency of the fifth component of complement ( C5). Paper-2726811.
We also identified a haplotype of the C5 gene that was protective against childhood BA (P = 1.4 x 10(-6)) and adult BA (P = 0.00063). Paper-10630759.
The deficiency of complement C5 is rare and frequently associated with severe and recurrent infections, especially caused by Neisseria spp. Paper-13796286.
The C5 alpha-chain was encoded by 49 kilobases containing 26 exons; the beta-chain was encoded by 29 kilobases containing 16 exons. Paper-34684.
The conversion is accompanied and probably caused by oxidation of methionine residues in the C5 protein to methionine sulfoxide. Paper-234531.
In contrast, T and B lymphocyte and complement ( C5) defects had little effect on the clearance of Escherichia coli from the kidneys. Paper-4872169.
In contrast, T and B lymphocyte and complement ( C5) defects had little effect on the clearance of Escherichia coli from the kidneys. Paper-4543506.
The C1 and C5 domains of gp120 are thought to directly interact with gp41 but are largely missing from the available X-ray structure. Paper-9561826.
Twenty-three synthetic peptides with C5 sequences and 10 with C3 sequences were also tested for the ability to inhibit C hemolytic activity. Paper-1865012.
Macrophages from such mice were unable to present biosynthesized C5 to class II-restricted T cells, even when preactivated in vivo. Paper-518375.
They contained taurine chloramine, estimated photometrically, and they activated C5 upon incubation, assayed functionally by reactive lysis. Paper-839510.
Recently, C5-derived peptides have been implicated as being mediators of acute lung injury (i.e., " shock lung") in some clinical situations. Paper-4302610.
The C5 structural gene was found to be a highly interrupted gene of approximately 78 kilobases containing 42 exons and 41 introns. Paper-34684.
The complex of activated C5 with C6 is capable of combining with the components C7, C8, and C9 forming the cytotoxic terminal complement complex C5-9. Paper-234531.
Generation of slow reacting substance by human leukocytes. II. Comparison of stimulation by antigen, anti-IgE, calcium ionophore, and C5-peptide. Paper-3647016.
A method for purification of C5 is described in which polyethyleneglycol (PEG) or (NH4)2SO4 precipitation, as well as low or high pH, was avoided. Paper-4520354.
Structural aspects of the human C5 gene. Intron/exon organization, 5'-flanking region features, and characterization of two truncated cDNA clones. Paper-43045.
Soluble histamine added to mouse peritoneal macrophages in culture suppressed the synthesis of the functional and antigenic complement component C5. Paper-4198438.
When taurine was omitted from the PMN suspensions during stimulations much less oxidant was found in the supernatants and less C5 activating potency. Paper-839510.
These findings provide direct support for previous evidence suggesting the biologic activity for neutrophils derives entirely from the alpha subunit of C5. Paper-3258549.
The C5a molecule is one of two spasmogenic fragments (i.e. C3a and C5a) released from serum components C3 and C5 during complement activation. Paper-3050930.
Chemotactic activity for human polymorphonuclear leukocytes was generated that was inhibitable by incubation with anti- C5, but not with anti-C3 antisera. Paper-3794470.
Because one potent chemotactic factor released by AMs is leukotriene B4 ( LTB4), we hypothesized that cell surface C5 may modulate LTB4 release. Paper-6828088.
Three of the C5 peptides and one of the C3 peptides showed 50% inhibition of both C hemolytic and bactericidal activities at a concentration of 100 microM. Paper-1865012.
Overall, the results suggest that regions important for function of both C3 and C5 lie proximal to residues 150-200 and 1600-1620 in the precursor sequences. Paper-1865012.
The DNA sequence showed that the pC5HG2 insert was comprised of 3309 base pairs of pro- C5 coding sequence and 404 base pairs of 3'-untranslated sequence. Paper-5936125.
Two truncated human C5 clones, pHC5A and pHC5B, were isolated from an adult human liver cDNA library, and contained inserts of 2930 and 2181 bp, respectively. Paper-6864082.
Truncated C5 mRNA were not detected in Raji, MOLT-4, human fibroblast or U937 cells, although the full-length 6.0-kb transcript was seen in MOLT-4 cells. Paper-6864082.
We have analyzed the role of complement component C5 in experimental autoimmune encephalomyelitis (EAE) using C5-deficient (C5-d) and C5-sufficient (C5-s) mice. Paper-10028711.
The results indicate that much of the random migration observed in the chemotaxis under agarose system is due to C5-derived peptides present in normal human serum. Paper-3758130.
In contrast to the recently described C5-dependent pathway in PMN receptor modulation by CU, this effect seemed to depend on the presence of the complement factor C3. Paper-8704602.
These labelling patterns provide evidence for the operation of the C5 pathway of 5-aminolevulinic acid synthesis for bacteriochlorophyll c formation in the bacterium. Paper-5389364.
Based on the experimental results, an intercalation model that permits competitive hydrogen abstraction from C5' and C4' of deoxyribose moiety has been proposed. Paper-6843399.
When added to sensitive target cells ( guinea pig or chicken erythrocytes), C5 mediated lysis by C7-C9 without the addition of C6 or any activation procedure. Paper-4520354.
Seven of these aptamers formed a closely related sequence homology family; these aptamers bound C5 with a Kd 20-40 nM and also inhibited human serum hemolytic activity. Paper-1970778.
Biophysical studies of the HIV gp120 C5 domain (residues 489-511 of HIV-1 strain HXB2), which corresponds to the carboxy terminal region of gp120, have been undertaken. Paper-9561826.
Human polymorphonuclear leukocytes (PMN) were stimulated in vitro by incubation with opsonized zymosan; thereafter the supernatants were tested for C5 activating potency. Paper-839510.
From the relative hydrophobicity, the hydrophobic sites of C5 can be localized to the alpha 2 fragment (Mr = 58 K) since C5FIII lacked the alpha 2 fragment and was not hydrophobic. Paper-5968950.
Experiments with mastocytoma cells showed that a hydrogen is stereospecifically eliminated from C-10 during the conversion of eicosapentaenoic acid to leukotriene C5. Paper-4356279.
However, near the 3'-ends, at exon/ intron boundaries, the nucleotide sequences of pHC5A and pHC5B diverged from each other and from the full-length 6.0-kb C5 cDNA sequence. Paper-6864082.
The oxidation product of methionine, its sulphoxide, may cause a change in structural conformation of C5 which involves expression of the C6 binding site. Paper-54587.
CD studies of the C5 domain suggest that it is unstructured in aqueous solutions but partially helical in trifluoroethanol/aqueous and hexafluoroisopropanol/aqueous buffers. Paper-9561826.
Furthermore, both wheezing phenotypes showed decreased expression of the complement component 5 receptor 1 gene, a gene involved in the regulation of bronchial responsiveness. Paper-13066783.
Monoclonal antibodies as probes to investigate the molecular changes of C5 associated with the different stability of the molecule on sheep erythrocytes and Escherichia coli 0111:B4. Paper-23852.
The suppression of C5 activity in resident and thioglycollate-stimulated cultures was dependent on the dose of histamine with maximal suppression occurring at a concentration of 10(-3) M. Paper-4198438.
Replication of the tumor necrosis factor receptor-associated factor 1/ complement component 5 region as a susceptibility locus for rheumatoid arthritis in a European family-based study. Paper-12987500.
C5 was identified on guinea pig alveolar macrophages by epifluorescent microscopy, flow cytometry, and enzyme-linked immunoabsorbent assay of eluted macrophages. Paper-5588658.
Thus, among the genes for complement components which can be mapped because of deficiency states or polymorphic gene products, C5 joins C1r, C3 and C6 in not being closely linked to HLA. Paper-2726811.
The deduced amino acid sequence of a partial cDNA clone (2.25kb), representing approximately 44% of the coding sequence, showed 60 and 58% similarity to human and mouse C5, respectively. Paper-8976402.
Examination of cytoplasmic RNA prepared from human liver and the human hepatoma cell line Hep G2 by Northern transfer has indicated a C5 mRNA species of about 5.2 kilobase pairs. Paper-5082442.
The high binding affinities suggest that complexes composed of C5 bound to C6 or C7 exist in plasma before activation and may facilitate assembly of the complement membrane attack complex. Paper-10102624.
The derived amino acid sequence contained the entire coding sequence of the C5 alpha-chain, the beta-alpha-chain junction region, and 100 amino acids (approximately 50%) of the beta-chain. Paper-5936125.
It was also found that 5(-14)C-labelled glutamate and 2-oxoglutarate were incorporated into these chlorophylls which is consistent with the operation of both the C5 and Shemin pathways. Paper-4042296.
This report describes additional site-specific mutagenesis and synthetic peptide inhibition studies aimed at testing this idea for the paralogous complement C3, C4, and C5 proteins. Paper-1865012.
The C5 cDNA clone was sheared randomly by sonication, subcloned into M13 mp8, and sequenced at random by the dideoxy technique, thereby generating a contiguous sequence of 1703 base pairs. Paper-5082442.
The serum glycoprotein C5a, which is derived from the proteolytic cleavage of complement protein C5, has been implicated in the pathogenesis of a number of inflammatory and allergic conditions. Paper-1057732.
Previously, we have stablished that the fifth component of complement ( C5) serves as an important source of mediators that have locomotory (chemotactic) activity for leukocytes and tumor cells. Paper-3334779.
Clone pHC5B encoded the entire C5 signal peptide, the beta-chain, the linker peptide, nine amino acids of the alpha-chain, and six amino acids of divergent protein sequence in an open reading frame. Paper-6864082.
Most importantly, C5 deficiency was excluded ( lod score greater than -2.0) from linkage with the major histocompatibility locus (HLA) from a recombination frequency of greater than 15% (in females). Paper-2726811.
The chemotactic factor, of which the apparent molecular mass was 75 kDa, shared antigenicity with complement C5 and possessed the affinity to monocytes, but not to polymorphonuclear leukocytes. Paper-7209512.
The defect in Leiner's disease, which presents in early infancy with extensive dermatitis, diarrhoea, and failure to thrive, has been attributed to a defect of the fifth component of complement ( C5). Paper-2795850.
CVF-B purified by gel filtration could initiate the hemolysis of unsensitized guinea pig erythrocytes when incubated with human complement components C5 to C9 in 0.01 M EDTA buffer. Paper-2312473.
The 2-base-pair gene deletion and HindIII and PvuII restriction fragment length polymorphisms associated with murine C5 deficiency were localized to exon 7, exon 16, and intron 20, respectively. Paper-34684.
Moreover, challenged C5-deficient mice had a 3.4-fold and 2.7-fold increase in the levels of airway eosinophils and lung interleukin (IL)-4-producing cells, respectively, compared with challenged wild-type mice. Paper-11371702.
It is intriguing to speculate, therefore, that circulating C5-derived chemotactic peptides may play a role in the pathogenesis of the lung injury observed in some patients during the course of acute pancreatitis. Paper-4302610.
Mycoplasma pulmonis inoculated parenterally into mice deficient in the fifth component of complement ( C5) caused a chronic arthritis of significantly greater magnitude than in immunologically normal mice. Paper-2991488.
Structure of the murine fifth complement component ( C5) gene. A large, highly interrupted gene with a variant donor splice site and organizational homology with the third and fourth complement component genes. Paper-34684.
The basophils of patients with asthma and control subjects responded similarly to most stimuli: they were significantly less responsive to C5-peptide and f-met peptide, and significantly more responsive to D2O. Paper-3611286.
OBJECT: The incidence of postoperative C-5 spinal nerve root palsy following decompressive cervical spinal surgery has been reported to be as high as 12% for anterior procedures and 30% for posterior procedures. Paper-13270275.
Fluorescence titration experiments suggest that HIV C5 forms a 1 : 1 complex with the HIV gp41 ectodomain in the presence of cosolvent with an apparent Kd of approximately 1.0 micro m. Paper-9561826.
Thus, the mouse quantitative trait gene led to the identification of an unknown gene underlying human susceptibility to liver fibrosis, supporting the idea that C5 has a causal role in fibrogenesis across species. Paper-10758238.
Regulation by H of formation of the C3 and C5 alternative pathway convertases of complement on cells is dependent on such chemical characteristics of the cell surfaces as their membrane content in sialic acid. Paper-4277144.
The fractional catabolic rate and metabolism of factor B in vivo is similar to that of C3, C4 and C5 complement proteins, which are among the most metabolically active plasma proteins in the circulatory system. Paper-6139292.
Culturing the AMs with anti- C5 Fab' antibodies inhibited the release of LTB4 induced by opsonized zymosan, immune complexes, or lipopolysaccharide (78%, 41%, and 82% inhibition, respectively, p less than 0.05). Paper-6828088.
These cells were challenged with several different stimuli: goat anti-human IgE-Fc, C5-peptide, N-formyl-methionyl-leucyl-phenylalanine (f-met peptide), Ca++ ionophore A23187, hyperosmolar mannitol, and D2O. Paper-3611286.
The chemotactic activity was heat-stable (56 degrees C for 30 min), inhibitable by treatment with antibodies to human C5, and exhibited an apparent molecular weight of 16,000 (determined by chromatography on Sephadex G-75). Paper-4302610.
The duplex stability of the ODN bearing isocytosine derivative with DNA was lower than that of the normal ODN/DNA duplex but the ODN bearing C5-substituted uracil derivative was similar to the normal ODN/DNA duplex. Paper-10958672.
The proof by 13C-NMR spectroscopy of the predominance of the C5 pathway over the Shemin pathway in chlorophyll biosynthesis in higher plants and of the formation of the methyl ester group of chlorophyll from glycine. Paper-4458527.
Platelet-rich-plasma from two hemostatically normal individuals, genetically lacking the fifth component of complement ( C5), failed to exhibit normal platelet aggregation, or serotonin release, in the presence of zymosan. Paper-2893033.
CONCLUSIONS: Transcranial electrical stimulation-induced MEPs and spontaneous EMG activity offer complementary information about evolving iatrogenic C-5 spinal nerve root impairment during anterior cervical spinal surgery. Paper-13270275.
Eculizumab, an antibody that targets complement fraction 5 ( C5), has proven highly effective in abolishing complement-mediated intravascular hemolysis in PNH; however, the hematologic benefit varies considerably among patients. Paper-13732096.
An N181S mutant lost affinity for adenosine agonists substituted at N6 or C-2, but not at C-5'. The mutant receptors I274A, S277A, and H278A showed full stimulation of adenylate cyclase at high concentrations of CGS 21680. Paper-252067.
PATIENTS: 80 ASA physical status I patients were randomized into four equal groups to receive oral premedication with either clonidine 5 mcg/kg ( C5), clonidine 2.5 mcg/kg (C2.5), diazepam 100 mcg/kg (D), or placebo (PL). Paper-1658736.
Direct sequencing of all C5 exons displayed no mutation of obvious functional significance, except for an A to G transition in exon 10 predicting an exchange from lysine to arginine. Paper-10734840.
Northern blot analysis of RNA from various tissues showed the presence of a single mRNA transcript in trout liver and Southern blot analysis indicated that the gene coding for C5 is present as a single copy in the trout genome. Paper-8976402.
Other marker systems excluded from linkage with C5 deficiency included the ceruloplasmin and Duffy loci at a recombination frequency of less than 15%, and the erythrocyte glyoxalase, MN, and Lewis loci at a recombination frequency of less than 5%. Paper-2726811.
Indeed, we have shown that hypocomplementation by cobra venom factor (CVF) dose-dependently attenuates LPS-induced fever in guinea pigs and wild-type (WT) mice, and that C5 gene-ablated mice are unable to develop fever after LPS. Paper-10244346.
Since hypochlorite and T-NCl are biological products generated by the myeloperoxidase-halide system of stimulated leukocytes, the activation of C5 by these agents may be one way to complement activation during inflammation and tissue injury. Paper-234531.
These findings provide the first direct evidence that neutrophils recruited to the skin via a C5-dependent pathway play an essential role in subepidermal blister formation in experimental BP, and suggest new directions for disease intervention. Paper-1148155.
In order to rule out interfering activities of proteolytic enzymes released from the PMN and known to attack C5, the supernatants were ultracentrifuged, and the ultrafiltrates, containing only low molecular weight compounds, were used for the further studies. Paper-839510.
A series of hexanucleotides possessing A-T, G-C, inosine (I)-C and 2-aminoadenine (ANH2)-T base pairs at 5'-side of the target thymine were prepared and their selectivity for C-5' and C4' oxidation in the NCS-mediated degradation was investigated. Paper-6843399.
We conclude that the two C5D individuals of the family reported here may not have sufficient C5 activity to provide efficient protection against Neisserial infections in conditions where complement functions beyond C3 opsonic activity are required in vivo. Paper-6609932.
Our results provide strong evidence that this binding corresponds to the previously described reversible binding of C5 to C6 and C7, and taken together with earlier work, indicate that the C5-C345C module interacts directly with the factor I modules in C6 and C7. Paper-10102624.
Recent identification of a C5-binding protein in the salivary gland of the soft tick Ornithodoros moubata has enabled development of a terminal pathway-specific reagent, OmCI, with potential to ameliorate disease while leaving key physiological processes unaffected. Paper-13144681.
The chemotactic activity in cerebrospinal fluid was heat stable (56 degrees C for 30 min), eluted from Sephadex G-75 with a profile identical to that of the chemotactic activity in zymosan-activated rabbit serum, and was inhibited by treatment with antibodies to native human C5. Paper-4646340.
However, the strains BcA67 and BcA72 showed discordant phenotypes with respect to their C5 status, suggesting additional complexity in the genetic control of the inter-strain difference in susceptibility observed in A/J and C57BL/6J following systemic infection with C. albicans. Paper-11470713.
Cleavage of 2' amino substituted substrates at different pH showed that substitution of Mg2+ by Mn2+ (or Ca2+), identity of residues at and near the cleavage site, and addition of C5 protein influenced the frequency of miscleavage at -1 (cleavage at the correct site is referred to as +1). Paper-10605826.
Complement activation as one source of BAL chemotactic activity was suggested by almost complete inhibition of BAL chemotactic activity after incubation with antibody to the fifth component of complement ( C5) and detection of C5a des Arg in BAL fluid from some subjects challenged with CBE. Paper-5271202.
This suggests that the C5 pathway of tetrapyrrole biosynthesis operates in both Zea mays and R. spheroides since in the alternative Shemin pathway the label would have been lost as 14CO2 during the formation of succinyl-CoA prior to the condensation with glycine to form 5-aminolaevulinate. Paper-4042296.
In this paper, we evaluate by immunochemical methods the C5 deficiency in a six-member family, in which one member suffered from meningococcal sepsis and several pneumonia episodes; and a second one with two bacterial meningitis episodes and frequent tonsillitis, pneumonia and herpetic episodes. Paper-10776372.
Recent findings indicate that distinct nucleotide sequences, termed exonic splicing enhancers (ESEs), influence the splicing process. cDNA from all family members harboring the mutated allele showed skipping of exon 10, which resulted in a premature STOP codon, explaining the lack of C5 in the propositus. Paper-10734840.
The isotope in the glutamate was specifically incorporated into the eight carbon atoms in the tetrapyrrole macrocycle derived from the C-5 of 5-aminolevulinic acid, while no specific enrichment of these eight carbon atoms was observed in the spectrum of the pigment formed in the presence of [2-13C]glycine. Paper-5389364.
On examination, there was a dermal sinus in the skin overlying the spine of the seventh cervical vertebra, and myelography with computed tomography scan demonstrated a block to the flow of metrizamide contrast material at T-5 with cephalad extension of an intradural cystic lesion to the C-5 level. Paper-4627721.
Of clinical interest are (a) the documentation of membranous glomerulonephritis, vasculitis, and arthritis in an individual lacking C5 (and its biologic functions), and (b) a remarkable propensity to bacterial infections in the proband, even during periods of low-dose or alternate-day corticosteroid therapy. Paper-2483366.
Using microarray analysis of pulmonary gene expression and single nucleotide polymorphism-based genotyping, combined with quantitative trait locus analysis, we identified the gene encoding complement factor 5 ( C5) as a susceptibility locus for allergen-induced airway hyperresponsiveness in a murine model of asthma. Paper-8542915.
The 6-step procedure, involving removal of C1 by precipitation at pH 7.5, mu = 0.04, 2.0 M ammonium sulfate precipitation, acid precipitation at pH 5.6 mu = 0.1, and successive chromatographies on Sephadex G-200, DEAE-cellulose, and hydroxylapatite columns, yielded 1.6 to 4 mg of C5 from 250 ml of serum. Paper-3740672.
The isotope in the glutamate was specifically incorporated into eight carbon atoms in the tetrapyrrole macrocycle derived from the C-5 of 5-aminolevulinic acid ( ALA), and the 13C in glycine was incorporated into the methyl carbon of the methoxycarbonyl group attached to the isocyclic ring of bacteriochlorophyll a. Paper-5386276.
When cultured for 4 h with stimuli that induce the release of chemotactic activity or for 24 h without added stimuli, purified Fab' fragments of a goat anti- C5 antibody significantly inhibited the ability of macrophages to release chemotactic activity as determined by a blindwell chamber method (p less than 0.001, all comparisons). Paper-5588658.
On March 16, 2007, eculizumab (Soliris; Alexion Pharmaceuticals, Inc. Cheshire, CT), a humanized monoclonal antibody that binds to the human C5 complement protein, received accelerated approval by the U.S. Food and Drug Administration for the treatment of patients with paroxysmal nocturnal hemoglobinuria (PNH) to reduce hemolysis. Paper-13013778.
Irradiation of the forearms of two patients with erythropoietic protoporphyria and one patient with porphyria cutanea tarda resulted in an in vivo activation of the complement system, as assessed by diminution of the hemolytic titers of the third component of complement by 23-57%, and of the fifth component of complement ( C5) by 19-47%. Paper-4553008.
The fifth component of human complement, C5, was activated by non-enzymical, chemical treatment in either of two ways: 1) by oxidation with a hydroxyl radical (OH.) generating system consisting of H2O2, FeEDTA, and ascorbate, activation product called C5(H2O2); 2) by oxidation with chloramine T, activation product called C5(Cl-T). Paper-7527900.
Biosynthesis of 5-aminolevulinic acid (ALA) in Chloroflexus aurantiacus, a thermophilic bacterium forming bacteriochlorophyll c, is shown to proceed via the C5 pathway by demonstrating (1) the specific labeling of its chlorin ring with [1 - 13C]glutamate and (2) the enzyme activity to produce ALA from glutamate in a cell-free extract. Paper-7084319.
Comparison of the average EMG activity of the paraspinal C-5 muscle in different phases of cervical traction showed a more significant decrease of EMG activity during the pull phase of traction as well as after traction in the high muscle tension group (with EMG activity above 5 microV), especially with the biofeedback traction modality. Paper-905204.
These synonyms are used for gene C5 (complement component 5): MGC142298, FLJ17822, FLJ17816, CPAMD4, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4.
These accession numbers are used for gene C5: Q27I61 (UNIPROT__AC), Q14CJ0 (UNIPROT__AC), AAA51925 (NCBI_GENBANK__AC), AAA51856 (NCBI_GENBANK__AC).
C5 is a homologue of LOC565774 (similar to complement component C5-1) from Danio rerio.
C5 is a homologue of Hc (hemolytic complement) from Mus musculus.
C5 is a homologue of C5 (complement component 5) from Bos taurus.
C5 is a homologue of C5 (complement component 5) from Pan troglodytes.
C5 is a homologue of C5 (complement component 5) from Gallus gallus.
C5 is a homologue of C5 (complement component 5) from Rattus norvegicus.
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