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This binding is prevented by DPI but not by L-NAME. Paper-13633515.
Pemphigus vulgaris with autoantibodies to desmoplakin. Paper-9098039.
Desmoplakin as a specific marker of lymphatic vessels. Paper-8829329.
Neither apocynin nor DPI affected leukocyte sequestration. Paper-8358634.
Intracellular distribution of desmoplakin in human odontoblasts. Paper-10751638.
Antibodies to desmoplakin in a patient with pemphigus foliaceous. Paper-1648949.
However, DPII mRNA could not be detected in cardiac muscle tissue. Paper-6754062.
Desmoplakin-positive true desmosomes were found in 5 specimens. Paper-5303976.
Desmoplakin is a member of the plakin family of IF-binding proteins. Paper-9521972.
Structure of desmoplakin and its association with intermediate filaments. Paper-7478304.
Mucosal dominant pemphigus vulgaris with anti- desmoplakin autoantibodies. Paper-10456313.
Molecular structure of the human desmoplakin I and II amino terminus. Paper-7237337.
Duration of immunity ( DPI) was established at 12 months post vaccination. Paper-12638292.
A DSP based multi-frequency 3D electrical impedance tomography system. Paper-12898428.
Striate palmoplantar keratoderma resulting from desmoplakin haploinsufficiency. Paper-2057775.
Loss of desmoplakin tail causes lethal acantholytic epidermolysis bullosa. Paper-10798337.
EGCG and DPI inhibited cytosolic subunits from translocating into membrane. Paper-13419409.
Autoantibodies to desmoplakin I and II in patients with erythema multiforme. Paper-146373.
The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. Paper-9521972.
Desmoplakin and Plakoglobin--specific markers of lymphatic vessels in the skin? Paper-10429339.
However, Desmoplakin did not stain several positively identified lymphatic vessels. Paper-10429339.
Novel mutation in desmoplakin causes arrhythmogenic left ventricular cardiomyopathy. Paper-11300468.
Erythema multiforme associated human autoantibodies against desmoplakin I and II. Paper-1785171.
Under current-clamp recordings, DPI prolonged action potential duration in GH3 cells. Paper-13055029.
The desmosome consists of several proteins, of which desmoplakin is the most abundant. Paper-8607286.
Detection of autoantibodies to desmoplakin in a patient with oral erythema multiforme. Paper-13236753.
Haploinsufficiency of desmoplakin causes a striate subtype of palmoplantar keratoderma. Paper-1757516.
Desmoplakin I & II are important components of the attachment plaque of desmosomes. Paper-8225142.
Expression of intermediate filaments and desmoplakin in vertebrate olfactory mucosa. Paper-5850809.
The catalytic domain of DSP18 adopts a fold similar to that known for other DSP structures. Paper-12005666.
The predicted amino acid sequence of DP was obtained by analysis of overlapping cDNA clones. Paper-7478304.
Twenty-six family members (11 males and 15 females) were found to carry a DSP mutation. Paper-11468456.
Inhibitors of NAD(P)H oxidase, apocynin and DPI, did not block E2-induced ROS formation. Paper-12001140.
Endothelial cells were more sensitive to the effects of DSP than smooth muscle cells. Paper-4357461.
Desmoplakin, another marker of epithelial differentiation, was not found in melanoma cells. Paper-6498831.
Salmonella was reisolated from different organs at 7, 14, 21, and 28 postinoculation ( DPI). Paper-8885845.
Body mass index and functioning in long-term schizophrenia. Results of the DSP project. Paper-13359407.
Superoxide production was measurable in all placenta tissues and was inhibited by DPI and TIRON. Paper-10548897.
RESULTS: After DSP treatment, the arterial endothelium was swollen and loosing its attachment. Paper-10808457.
The DPI device is a multiple-dose inhaler with a counter containing agglomerates of MF and lactose. Paper-12590166.
Downregulation of desmoplakin in cardiomyocytes from HM was also seen on the protein level. Paper-13083751.
Nevertheless, it had not yet been demonstrated that DP is required for attaching IF to the desmosome. Paper-645581.
At 0.15 mM, desmosome formation occurs, and desmoplakin is associated with the desmosomal plaque. Paper-6496074.
Desmoplakin expression and organization at human umbilical vein endothelial cell-to-cell junctions. Paper-882562.
Autoantibodies against desmoplakin I and II define a subset of patients with erythema multiforme major. Paper-534124.
Changes in the concentration and pH value of DPI solutions do not significantly affect the NMR spectra. Paper-11860249.
DPI may also increase intracellular Ca2+ by directly altering sarcoplasmic reticulum Ca2+ handling. Paper-5957601.
Simultaneously, they started to express desmoplakin, form desmosomes and flatten on the culture substratum. Paper-1511940.
We have used transient RNA interference (RNAi) to downregulate Dp specifically in HaCaT keratinocytes. Paper-13294309.
Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis. Paper-8426423.
RESULT: Immunofluorescence for desmoplakin confirmed desmosome presence throughout all three epithelia. Paper-2060078.
DPIP inhibited NADPH-d activity in all regions except for the olfactory/ vomeronasal nerve and glomeruli. Paper-727374.
RESULTS: We found no qualitative correlation of desmoplakin or plakoglobin expression with risk of metastasis. Paper-10175411.
We showed that this Dp downregulation also caused reduced expression of several other desmosomal proteins. Paper-13294309.
CONCLUSIONS: This is the first description of a phenotype caused by a mutation affecting only one DSP isoform. Paper-11388475.
Furthermore, no significant correlation could be detected between Leydig cell numbers and DSP in these 50 men. Paper-5608940.
Serum potassium, glucose and formoterol plasma profiles were comparable for the CFC, HFA and DPI devices. Paper-11414567.
Within the immune system, PGD(2) binding to DP generally leads to suppression of cellular functions. Paper-13396678.
Distribution of desmoplakin in normal cultured human keratinocytes and in basal cell carcinoma cells. Paper-6496074.
The working conditions (mediator concentration, applied potential and pH) were optimised for both DPIP and PMS. Paper-11828231.
The above effects of DPI on human neutrophils are similar to those found in chronic granulomatous disease. Paper-5803110.
There was a loss of punctate staining evident when using a desmosome plaque protein ( desmoplakin) antibody. Paper-1611644.
DPI normalized for body weight (DPIN) increased significantly from 0.75 +/- 0.1 to 1.02 +/- 0.18 (p = 0.02). Paper-7136426.
DPI 201-106 ( DPI) as a positive inotropic drug might be useful in treating dilated cardiomyopathy (DCM). Paper-5401568.
Liver metastases were detected with DPI, CT, US, and laparotomy in 56, 45, 27, and 36 patients, respectively. Paper-180461.
Each section was stained either with a monoclonal antibody specific for desmoplakin or with hematoxylin and eosin. Paper-8479004.
Increased keratinocyte proliferation initiated through downregulation of desmoplakin by RNA interference. Paper-13294309.
Therefore, different DSP mutations might produce different clinical phenotypes, with different modes of inheritance. Paper-9635078.
Evidence shows that the loss of desmoplakin in vivo results in leaky capillaries and/or capillary malformation. Paper-10500699.
The cytoplasmic side of the plaque is attached to intermediate filaments through desmoplakin, a major plaque protein. Paper-1571145.
DPI inhibition of the oxidase was associated with defective aerobic killing of staphylococci by human neutrophils. Paper-5803110.
Among them, 2,6-dichlorophenol indophenol ( DPIP) and phenazine methosulfate (PMS) were chosen for further development. Paper-11828231.
Paraneoplastic pemphigus is associated with autoantibodies directed against the desmosomal plaque protein, desmoplakin. Paper-1488553.
The second reaction pathway found for DPI and DPB in DNA is addition of an aryl radical to the thymine 5,6 double bond. Paper-1418397.
This study demonstrates the importance of desmoplakin in the attachment of intermediate filaments to the desmosome. Paper-8607286.
Exons of the desmoplakin gene were analyzed by single-strand conformational polymorphism and direct sequencing. Paper-9783674.
The DPI and DFR values of controls and patients with overt liver metastases were clearly separated (p less than 0.0001). Paper-7012683.
Unique epidermolytic bullous dermatosis with associated lethal cardiomyopathy related to novel desmoplakin mutations. Paper-13705964.
Both in monolayers and in vesicles reductive cleavage of the disulfide bond of DSP could be obtained by glutathione (GSH). Paper-11341581.
Over 73% of the variation in DSP/g could be attributed to variation in the percentage loss during postprophase of meiosis. Paper-4343362.
Mutations in genes encoding the cell adhesion proteins like plakoglobin and desmoplakin were related with these syndromes. Paper-12453656.
Intermediate-filaments are tissue-specific molecular cytoskeletal markers; desmoplakin is the major desmosomal protein. Paper-5500015.
Analysis of the desmoplakin gene reveals striking conservation with other members of the plakin family of cytolinkers. Paper-2069357.
Six GbNV ORFs are homologous to nonconserved baculovirus genes dnaligase, helicase 2, rr1, rr2, iap-3, and desmoplakin. Paper-13207993.
The CAN ( Control Area Networks) embedded module in the DSP provides robust transmission of information within the system. Paper-12412433.
Differentiation of human trophoblast cells in vitro as revealed by immunocytochemical staining of desmoplakin and nuclei. Paper-6836186.
Edema disease was prevented with the low- and high-dose Stx antibody treatments administered at 2 and 4 DPI, respectively. Paper-8682988.
The effects of DPI on ion currents and membrane potential in pituitary tumor (GH3) cells were investigated in this study. Paper-13055029.
JD-1 cells from a patient with a DP COOH-terminal truncation were also more weakly adherent compared with normal keratinocytes. Paper-9313508.
The immunohistochemical expression of desmoplakin and its role in vivo in the progression and metastasis of breast cancer. Paper-8275391.
Epidermal growth factor suppresses induction by progestin of the adhesion protein desmoplakin in T47D breast cancer cells. Paper-10311296.
Clinical profile of four families with arrhythmogenic right ventricular cardiomyopathy caused by dominant desmoplakin mutations. Paper-11468456.
However, parenchymal weights and numbers of epididymal spermatozoa were similar (p greater than 0.05) between DSP/ T groups. Paper-5999821.
Involvement of desmoplakin phosphorylation in the regulation of desmosomes by protein kinase C, in HeLa cells. Paper-1986931.
Using a similar approach, we have now investigated the behavior of ectopically expressed full-length DP I in cultured cells. Paper-7601823.
An inhibitor of nitric oxide synthase, dichloroindophenol ( DPIP), was used to distinguish the characteristics of NADPH-d activity. Paper-727374.
Percentage Hepatic Replacement (PHR) by metastases, measured using a computed tomography scanner, did not correlate with DFR or DPI. Paper-7766952.
L' was inhibited by tetrodotoxin and ryanodine, suggesting that DPI acts at both the sarcolemma and the sarcoplasmic reticulum. Paper-5957601.
In the present study, desmoplakin was localized to intercellular junctions in human dermal microvascular endothelial cells. Paper-1655732.
Monoclonal antibodies against Desmoplakin and Plakoglobin were tested for their suitability as specific markers of lymphatic vessels. Paper-10429339.
Desmoplakin as one of the desmosomal plaque components has proven to be a reliable marker for diagnosis of meningeal tumors. Paper-10001726.
Based on morning peak expiratory flow rate, once-daily MF DPI 400 microg was more effective than placebo (P < or = .001) at endpoint. Paper-2200304.
Background: Desmoplakin plays a vital role in cell adhesion, linking the transmembrane desmosomal complex to the cytoskeletal network. Paper-13705964.
The binding of probes to liposomes leads to an increase in fluorescence quantum yield as compared to organic solvents ( DSP) and water. Paper-4750002.
Nicotine, an important compound in cigarette smoke had, in an equivalent concentration as in DSP, no such effects (p > 0.05). Paper-10808457.
LV efficiency increased with DPI (group A, +22%; group B, +55%), but myocardial oxygen consumption (MVO2) did not change significantly. Paper-5401568.
Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. Paper-11092400.
The expression of intermediate filaments (IF) and desmoplakin was investigated in frog, bovine, and human (fetal) olfactory mucosa. Paper-5850809.
Recently, autoantibodies to desmoplakin I and II have been identified in a subset of patients with a severe form of erythema multiforme. Paper-10140189.
Results: This complex cardiocutaneous phenotype is associated with compound heterozygosity for two novel nonsense desmoplakin mutations. Paper-13705964.
Here, we report a patient with severe fragility of skin and mucous membranes caused by genetic truncation of the DP tail. Paper-10798337.
After a 1-year follow-up, 23 patients had developed liver metastases, all of which were predicted on the basis of an abnormal DPI value. Paper-180461.
Similarly, DP, detected on the cell membrane in the control, shifts into the cytosol of the keratinocytes following treatment. Paper-8427963.
Consequently, the goal of this study was to determine the function of DP in complexus adherens junctions during capillary formation. Paper-10500699.
CONCLUSION: Results suggest that DCDS measurement of the DPI is the most sensitive technique in detection of colorectal liver metastases. Paper-180461.
Antibodies to intermediate filaments and to desmoplakin were used to investigate the histogenetic origin of the olfactory neuroepithelium. Paper-5500015.
Desmoplakin I/II are found only in the detergent insoluble fraction suggesting that most of the protein is linked to the cytoskeleton. Paper-882562.
CONCLUSIONS: DPI can be used to identify patients with colorectal cancer at high risk of recurrence who are in need of adjuvant treatment. Paper-2065256.
A series of c-myc-tagged mammalian expression vectors encoding specific predicted domains of DPI were transiently expressed in COS-7 cells. Paper-7478304.
However, immunoblotting revealed a significant increase in DPI and II levels in the insoluble protein pool during desmosome formation. Paper-6754062.
DPI may produce an increase in intracellular Ca2+ by increasing intracellular Na+ and altering Na+-Ca2+ exchange across the sarcolemma. Paper-5957601.
Conversely, when linked to a foreign protein, as few as 86 NH2-terminal DP residues are sufficient to target to desmosomes efficiently. Paper-1459993.
Two-hybrid analysis reveals fundamental differences in direct interactions between desmoplakin and cell type-specific intermediate filaments. Paper-1135127.
Northern blot analysis revealed DPI and II mRNA to be present in all cell lines as well as simple and stratified epithelial tissues. Paper-6754062.
The widening of action potentials induced by DPI in these cells may be explained mainly by its block of I(K(DR))) in a state-dependent manner. Paper-13055029.
The crabs were fed with mussels containing DSP toxins (2500mug total okadaic acid equivalents/kg) for 17 days and then fasted for 19 days. Paper-12747878.
Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: II. Different types of lymphatic vessels. Paper-8037199.
The mutation was a heterozygous G > A transition at the donor + 1 site of intron 7 of the desmoplakin gene (939 + 1 G > A; Genbank M77830). Paper-2057775.
Various measurements related to testicular size were positively but weakly correlated with DSP/ testis (values for r ranged from +0.45 to +0.66). Paper-3578818.
MATERIALS AND METHODS: DCDS measurement of the DPI and CT, US, and laparotomy were performed in 161 consecutive colorectal cancer patients. Paper-180461.
Despentapeptide (des-B26-B30) insulin ( DPI), an active modified insulin, has been crystallized in the presence of 20% acetic acid pH 2. Paper-11812458.
These experiments suggested that the DP carboxyl-terminal domain is involved either directly or indirectly in linking IF with the desmosome. Paper-7601823.
A heterozygous, single adenine insertion (2034insA) in the desmoplakin gene was identified in affected individuals only. Paper-11300468.
Study II: AUC ratios were 97.9% (budesonide) and 82.2% ( formoterol) (budesonide/ formoterol pMDI versus budesonide pMDI+formoterol DPI). Paper-13014353.
Results were compared with inotropic responses to DPI 201-106 ( DPI, 0.1-3 mumol/l), Ca2+ (1.8-15 mmol/l) and isoprenaline (0.001-1 mumol/l). Paper-7169528.
The effect of Ca2+ on the DPI phosphorylation was further enhanced by the addition of ubiquitous Ca2+-dependent regulator protein calmodulin. Paper-4939739.
1. DPI 201-106 ( DPI) is a novel inotropic agent, with Na+ channel agonistic action combined with a sensitization of contractile proteins to Ca++. Paper-5644973.
Desmoplakin is an intracellular component of desmosomes which plays a role in the anchorage of intermediate filaments to these structures. Paper-882562.
Role of the retinoblastoma (pRb)-E2F/ DP pathway in cancer chemopreventive effects of green tea polyphenol epigallocatechin-3-gallate. Paper-9362346.
Desmosomes (spots reactive with desmoplakin antibodies) were present at the lateral membranes of the amnion epithelial cells. Paper-6088827.
At a Ca2+ concentration of 0.13 mM desmoplakin is arrayed like beads on a "string" of intermediate filaments at areas of cell-cell association. Paper-6496074.
Clear separation of the DPI and DFR values of controls and patients with histologically confirmed liver metastases was observed (P less than 0.001). Paper-6950114.
Pemphigoid, pemphigus and desmoplakin as antigenic markers of differentiation in normal and tumorigenic mouse keratinocyte lines. Paper-6247995.
In GD, HHD and DD, immunostaining showed a loss of desmoplakin I and II and plakoglobin from the desmosomes, and a diffuse staining in the cytoplasm. Paper-751455.
Furthermore, the potential of DP to interact with IF proteins in yeast seems to be regulated by phosphorylation of Ser 2849 within its COOH terminus. Paper-9894414.
Reduced expression of DP has been correlated with the progression of several cancers, but its role in in vivo breast cancer is yet to be established. Paper-8275391.
Desmosomal plaque proteins are preserved in all grades of breast cancer. An immunohistochemical study utilizing monoclonal antibodies to desmoplakin. Paper-5872162.
Platelets incubated with LDL cholesterol showed an increased O2- production, which was significantly inhibited by AACOCF3 (-78%) and DPI (-56%). Paper-9473066.
We have compared the expression of DPI and II at the mRNA and protein levels in cell lines derived from simple, transitional and stratified epithelia. Paper-6754062.
Specificity of superoxide generation by NAD(P)H oxidase was assessed using the inhibitors L-NAME, rotenone, allopurinol, DPI and TIRON. Paper-10548897.
Comparative genomic hybridization of squamous cell carcinoma of the esophagus: the possible involvement of the DPI gene in the 13q34 amplicon. Paper-1868234.
Furthermore, NaHS enhanced inhibitory effects of NAC, GSH, DPI, SOD, L-NAME, or vitamin C on oxidized DCF or O2- formation induced by HCY. Paper-12337670.
The desmosomal component desmoplakin plays a key role in tethering various intermediate filament networks through its C-terminal plakin repeat domain. Paper-12334364.
In this study, we assessed the involvement of the retinoblastoma (pRb)-E2F/ DP pathway as an important contributor in the antiproliferative effects of EGCG. Paper-9362346.
A confocal image by laser-scanning microscopy demonstrated the diffuse distribution of DPK in the cytoplasm throughout the odontoblast processes. Paper-10751638.
As cells aggregated, desmoplakin was redistributed and formed linear, punctate arrays at regions of cell-cell contact, consistent with desmosome formation. Paper-6836186.
Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Paper-7614960.
Involvement of the retinoblastoma (pRb)-E2F/ DP pathway during antiproliferative effects of resveratrol in human epidermoid carcinoma (A431) cells. Paper-9078928.
Each participant with SD- DPI was matched by age, gender, and type of speech involvement to 3 comparison speakers with speech delay of unknown origin (n = 87). Paper-11472401.
Transparent, barely visible sarcocysts found in tail, limbs and tongue striated muscles of the geckoes were 175-200 microns x 35-50 microns in size at 78 DPI. Paper-8609658.
Truncation of the carboxy terminus of desmoplakin and consequent disruption of intermediate filament binding may account for the predominant LV phenotype. Paper-11300468.
Immunosuppression and transplantation have been reported to induce porokeratosis (PK), especially its variant, disseminated superficial PK ( DSP). Paper-8541183.
To our knowledge, this is the first study showing the involvement of the pRb-E2F/ DP pathway as a mechanism of the cancer-chemopreventive effects of resveratrol. Paper-9078928.
Desmoplakin I and II are important components of the attachment plaque of the desmosome which mediates cell to cell adhesion, in epithelial cells. Paper-35303.
We found significant differences between DP-treated and nontreated knockout mice in calculi size and in urinary cystine excretion. Paper-13414530.
It is considered a disease of cell adhesion because mutations in desmosomal genes, desmoplakin and plakoglobin, have been implicated in the pathogenesis of ARVC. Paper-10827523.
Erythema multiforme associated human autoantibodies against desmoplakin I and II: biochemical characterization and passive transfer studies into newborn mice. Paper-1565375.
Using a monoclonal antibody specific for desmoplakin, we evaluated 52 breast carcinomas, 25 normal tissues, 10 benign breast lesions, and 14 nonepithelial tumors. Paper-5872162.
In addition, we showed that amino acids 1-34 in the amino terminus were necessary for subsequent recruitment of desmoplakin to the membrane and desmosome assembly. Paper-12041638.
The periodicity of acidic and basic residues in the rod suggests that DPI will aggregate with itself or similar molecules into higher order filamentous structures. Paper-6576149.
With cholesteatomas >5 mm, the sensitivity and specificity of DWI reached 100% and 88%, respectively, with values for DPI reaching 100% and 80%, respectively. Paper-12908640.
Desmoplakin is the principal plaque protein of desmosomes, specialized adhesion junctions found in various tissues including skin, heart and meninges. Paper-10733422.
We now report a further case of a desmoplakin mutation in a proband with striate palmoplantar keratoderma that also results in a null allele and haploinsufficiency. Paper-2057775.
DP IV has been studied extensively in disease and in the immune system by the use of enzyme assays which detect hydrolysis of Gly-Pro or Ala-Pro substrate. Paper-8655990.
For qualitative characteristics, alpha-methyldopa showed an ECC mechanism in terms of electron transfer reaction (placing it in DPI zone) at carbon paste electrode. Paper-11299030.
Here we provide evidence for the involvement of the pRb-E2F/ DP pathway as an important contributor of resveratrol-mediated cell cycle arrest and apoptosis. Paper-9078928.
Therefore, Desmoplakin and Plakoglobin antibodies are not specific markers of lymphatic vessels in the skin and the staining pattern is tissues and species dependent. Paper-10429339.
The total amount of desmosomal proteins applied was adjusted to give the same degree of staining intensity for desmoplakin, a cytoplasmic plaque protein of desmosomes. Paper-1274739.
We show that reduction of Dp staining correlates with loss of differentiation of the primary tumour, degree of invasion and presence of lymph node metastases. Paper-577712.
Reaction was also observed with a myocardial polypeptide from bovine and human hearts which had a similar Mr value (250,000) and isoelectric pH range as desmoplakin I. Paper-3973669.
This glycoprotein represents a general component of true desmosomes and it is coexpressed with obligatory desmosome-specific plaque proteins such as desmoplakin I. Paper-5303809.
This study indicates that Dp may participate in the regulation of keratinocyte cell proliferation by, in part at least, regulating cell cycle progression. Paper-13294309.
The new dramatic phenotype, which we named "lethal acantholytic epidermolysis bullosa," underscores the paramount role of DP in epidermal integrity. Paper-10798337.
Methods: Prospective cohort study with a six year follow-up of 126/158 adolescents who had taken part in a randomised trial of a family intervention following DSP. Paper-12218882.
Both types of FDC-LC contained desmoplakin immunoreactivity in a typical punctate pattern corresponding to intercellular junctions when tested with a specific antibody. Paper-6381268.
DPI scores were more closely related to study difficulty in the patients than controls; and served particularly to characterize their sylbism and work satisfaction. Paper-2769774.
This is in striking contrast to epithelial cells, where desmoplakin and alpha -+catenin are restricted to desmosomes and adherens junctions, respectively. Paper-1655732.
Desmoplakin I and II, which were present in the cytoplasm of keratinocytes in the low Ca2+ medium, moved to the cell surface after the medium was changed to MEM. Paper-7571369.
It is caused by a recessive mutation in desmoplakin, an intracellular protein that links desmosomal adhesion molecules to intermediate filaments of the cytoskeleton. Paper-11401487.
Whereas decreased Dp and Pg expression was closely associated with distant metastasis formation, reduced Pg expression was correlated to the development of large tumours. Paper-1966164.
In addition, spinous cells in epidermis are reactive with a mouse monoclonal antibody to desmoplakin, a desmosomal component immunologically distinct from pemphigus. Paper-6247995.
While most cell lines exhibited typical intercellular and in many cases cytoplasmic DP staining, T24 cells exhibited predominantly diffuse and dotty cytoplasmic staining. Paper-6754062.
We also observed markedly decreased amounts of specific immunoreactive signal for desmoplakin, plakoglobin, and the gap junction protein, connexin43, at intercalated disks. Paper-10447934.
Immunohistochemical labelling of desmoplakin was used as a marker for desmosomes, and of connexin43 as a marker for gap junctions, in single and double stainings. Paper-773185.
In a previous report, we described autoantibodies against the desmosomal plaque proteins desmoplakin I and II (dp I and II) in patients with erythema multiforme (EM) major. Paper-534124.
We have shown pronounced differences in the efficacy and potency of the enantiomers of DPI 201-206 and BDF 9148, which may be useful for future radioligand binding studies. Paper-7705977.
Using specific inhibitors of DP IV, it has been shown that this enzyme is involved in the regulation of DNA synthesis and in production of various cytokines in lymphocytes. Paper-8655999.
Case report of a Spanish patient with arrhythmogenic right ventricular cardiomyopathy and palmoplantar keratoderma without plakoglobin and desmoplakin gene modifications. Paper-13196565.
The integrated system was then applied to analyze the electrostatic charges of a DPI formulation composed of salbutamol sulphate-Inhalac 230(R) dispersed using a Rotahaler(R). Paper-13022203.
To accomplish this goal, we used siRNA technology to knock down desmoplakin expression in endothelial cells before they were induced to form microvascular tubes on matrigel. Paper-10500699.
Co-expression of GFP and DSP proteins in the odontoblast layer was detected in the recovered implants, suggesting that GFP+ FDPMCs can function as odontoblasts in vivo. Paper-13534889.
Mutations in genes encoding the cell adhesion proteins piakoglobin and desmoplakin that truncate the proteins at the C-terminal domains were identified to underlie this syndrome. Paper-10488200.
We report here that, despite the absence of desmosomes, cultured endothelial cells from human umbilical vein express desmoplakin I and II both at mRNA and protein level. Paper-882562.
It is caused by a recessive deletion mutation in desmoplakin, an intracellular protein that links desmosomal adhesion molecules to intermediate filaments of the cytoskeleton. Paper-10447934.
Our study demonstrates that most infiltrating breast carcinomas retain abundant desmoplakin, regardless of tumor grade, degree of differentiation, or tumor type (duct or lobular). Paper-5872162.
Persistent calculi are treated with thiols [i.e., d-penicillamine ( DP) and mercaptopropionylglycine ( MPG)] for cystine solubilization. Paper-13414530.
In cultured human keratinocytes (NHEK) maintained in medium containing low levels of Ca2+ (0.04 mM) desmoplakin is a component of certain electron-dense bodies in the cytoplasm. Paper-6496074.
In general, the injection of DPI into nucleus accumbens produced greater behavioral change in animals with hippocampal damage than in animals with either neocortical or sham lesions. Paper-4089363.
The indole component accepted electrons from radical intermediates of the light excited dye; the piperazine part of DPI acted as an electron donor in the same radical reactions. Paper-1111110.
The DPI values for a third group of subjects who had undergone resection for colonic cancer but who had no proven liver metastases overlapped the values for the other two groups. Paper-7463580.
Similarly, the extent of syncytialisation of term trophoblasts, quantitated by nuclei in desmoplakin-negative areas, was highest upon addition of hCG-A or recombinant EGF as a control. Paper-12381999.
Upon elevation of the level of Ca2+ in the culture medium to 1.2 mM, desmoplakin first appears at sites of cell-cell contact in association with bundles of intermediate filaments. Paper-6496074.
CONCLUSION: In this study, the combination of nebulized arformoterol 15mug BID plus tiotropium 18mug DPI QD was the most effective in improving pulmonary function and disease symptoms. Paper-13642984.
Additionally, the CO/NO scavenger hemoglobin (Hb) and CO specific synthetic inhibitor ZnPPIX, ASA and DPI reversed the darkness-induced stomatal closure and H(2)O(2) fluorescence. Paper-13511999.
In the circular apparatus, animals were observed after unilateral injection of the dopamine agonist, 3,4-dihydroxyphenylamino-2-imidazoline ( DPI) or saline into nucleus accumbens. Paper-4089363.
The 250-kd polypeptide comigrated with desmoplakin I (a protein found in the desmosomes of all epithelia), and the 230-kd antigen comigrated with the antigen of bullous pemphigoid. Paper-6634295.
In addition, we investigated whether changes in DPI and II expression occurred following calcium-induced cell contact formation and stratification in the human pharyngeal cell line, FaDu. Paper-6754062.
The identity of this desmoplakin clone was confirmed by demonstrating that antibody affinity purified on the protein product of the clone bound desmoplakins 1 and 2 in Western blots. Paper-6957216.
Epididymal transit time was calculated as the number of spermatozoa in a given region of the epididymis or in the entire epididymis divided by DSP/T of the connected testis. Paper-5999821.
To investigate the effect of an enhancer on the in vitro permeation, oleyl alcohol, 1-dodecyl-2-pyrrolidone ( DP), and lauric acid were used as enhancers. Paper-12692811.
Twenty-five primary molars each were restored with Galloy and DPI alloy respectively and evaluated, over a period of three months, for various aspects of corrosion in the oral cavity. Paper-8753595.
Increased cell proliferation and enhanced G(1)-to-S-phase entry in the cell cycle, as monitored by colonial cellular density and BrdU incorporation, were seen in Dp RNAi-treated cells. Paper-13294309.
The autoantibodies from these patients reacted with an antigen complex composed of desmoplakin I and the 230-kd antigen of bullous pemphigoid and two as yet unidentified epithelial antigens. Paper-6634295.
Aryl radicals generated from aromatic onium salts such as Ph3S+, and especially DPI and DPB, are found to be the agents which best abstract hydrogen atoms from the deoxyribose portion of DNA. Paper-1418397.
However, specific changes in the expression pattern of these proteins as well as desmoplakin I/II were detected by immunohistochemical staining in epithelial cells of menstrual endometria. Paper-327907.
The presence of keratins 7 and 19 and desmoplakin is highly associated with morphologically observable epithelial differentiation restricted to synovial sarcoma among spindle cell sarcomas. Paper-6950801.
6. In aortae from L2LPL mice an increased *O(2)(-) release occurred that was normalized by removing the endothelium and by the NAD(P)H oxidase inhibitor DPI and the PKC inhibitor GF109203X. Paper-9354098.
Subsequently, desmoplakin becomes incorporated into desmosomes in a manner comparable to that seen in mouse keratinocytes (Jones and Goldman: Journal of Cell Biology 101:506-517, 1985). Paper-6496074.
Desmoplakin I and II in acantholytic dermatoses: preservation in pemphigus vulgaris and pemphigus erythematosus and dissolution in Hailey-Hailey's disease and Darier's disease. Paper-35303.
Indeed, to date a variety of such markers have been proposed, including basement membrane components, constituents of junctional complexes such as desmoplakin and enzymes such as 5'-nucleotidase. Paper-9024245.
The regimen consisted of DCP/ DP repeated in exactly 28-day cycles, along with 50 mg cyclophosphamide per day, insistence on completing the treatment and avoiding irregular pulses in all patients. Paper-12855696.
Immunoblotting and immunoprecipitation demonstrated the presence of DPI and II in all cell lines at the whole-cell protein level as well as in association with cytoskeletal fractions. Paper-6754062.
The scavengers tested included neutral ( acrylamide, iodoacetamide) and cationic [ triphenylsulfonium (Ph3S+), o,o'-diphenylenebromonium (DPB) and o,o'-diphenyleneiodonium ( DPI)] compounds. Paper-1418397.
Analysis of a biopsy sample of the right ventricle from the proband showed markedly decreased localization of plakoglobin, desmoplakin, and connexin43 at intercalated discs in cardiac myocytes. Paper-12518973.
The resultant (15)N chemical shift correlates well with substituent constants, both in the simple Hammett or DSP relationships and the (13)C substituent-induced chemical shifts of the CN carbon. Paper-12302629.
The results show that proteins whose size, charge, and biochemical behavior are very similar to those of desmoplakin I and band 5 protein of cow snout epidermis are present in all desmosomes examined. Paper-4828994.
DSP encompasses approximately 45 kb organized into 24 exons and 23 introns, and the pattern of intron-exon borders bears a striking resemblance to other members of the plakin family. Paper-2069357.
These observations indicated a possible recruitment of soluble DPI/II into an insoluble pool after induction of desmosome assembly by the calcium switch, consistent with earlier reports for MDCK cells. Paper-6754062.
Mutations of DP may cause striate palmoplantar keratoderma, arrhythmogenic right ventricular dysplasia, skin fragility/woolly hair syndrome, Naxos-like disease, and Carvajal syndrome. Paper-10798337.
Chronic and subchronic toxicity following exposure to the DSP (Diarrhetic shellfish poisoning) toxin okadaic acid (OA) is receiving increasing attention as a public human health biohazard. Paper-13264664.
Kay Elemetric's Visi-Pitch, DSP 5500 Digital Spectrograph, and Nasometer are representative of these new instruments and are used as illustrations in the discussion of the assessment of speech acoustics. Paper-7086521.
Neighbor proteins were cross-linked with a cleavable homobifunctional reagent dithio-bis-(succinimidyl propionate) ( DSP) and solubilized before immunoprecipitation by anti-HLA monoclonal antibodies. Paper-5412884.
In both control and myopathic muscles, DPI produced concentration-dependent increases in action potential duration, resting muscle tension, peak isometric tension, and duration of isometric tension. Paper-5957601.
In myopathic muscles, 1 microM DPI sensitized the myofilaments to Ca2+, as evidenced by a significant shift of the [Ca2+]-force relationship towards lower [Ca2+], in both skinned and intact preparations. Paper-7442413.
Desmoplakins I and II ( DPI and II) are highly related proteins localized in the innermost part of the desmosomal plaque and are candidates for linking intermediate filaments (IF) to the desmosomal complex. Paper-6754062.
In addition, DP-NTP colocalized at cell-cell borders with alpha-catenin in the L-cell lines, and endogenous desmoplakin and alpha-catenin colocalized in cultured dermal microvascular endothelial cells. Paper-1655732.
After 24 h of culture, the cell preparations demonstrated spontaneous formation of microvilli and formation of small syncytial units as assessed by desmoplakin staining and FITC-dextran microinjection. Paper-1170194.
With respect to the intermediate filament-desmosome system, both cell types expressed desmoplakin I, but the vast majority of RL95-2 lacked well-formed desmosomes as demonstrated by electron microscopy. Paper-743884.
This activation by DPI is attributed to more favorable incorporation of P-450scc in these membranes and is consistent with previously reported effects of acidic phospholipids on other mitochondrial proteins. Paper-4460335.
In summary, the data suggest that loss of DP may be of potential importance in progression of breast cancer in vivo from normal, DCIS, well differentiated through to poorly differentiated, large tumours. Paper-8275391.
We find that, as expected, desmoplakin DPI/II and plakoglobin are expressed throughout the epidermis, gradually accumulating during differentiation, which probably reflects the increased numbers of desmosomes. Paper-7710295.
Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly. Paper-645581.
In keratinocytes, desmoplakin translocates from the cytoplasm to the plasma membrane following both high calcium switch and protein kinase C (PKC) activation by 12-O-tetradecanoylphorbol-13-acetate (TPA). Paper-188808.
When cholesterol was present in DMPC vesicles at 1:15 molar ratio, cardiolipin and L-alpha-phosphatidylinositol 4-monophosphate ( DPI) increased side chain cleavage by at least 5-fold (0.7 min-1-3.5 min-1). Paper-4460335.
Here we provide the first biochemical evidence that, in vivo, gamma-catenin also mediates interactions between classical cadherins and the intermediate filament cytoskeleton, linked through desmoplakin. Paper-12870956.
Transient transfection of a clone encoding full-length DP I into COS-7 cells produced protein that appeared in some cells to associate with desmosomes and in others to coalign with and disrupt IF. Paper-7601823.
The present study was designed to investigate the effect of lipid-soluble smoke particles ( DSP) on alpha-adrenoceptor expression in organ culture of rat mesenteric arteries and human epiploon arteries. Paper-12617110.
MATERIALS AND METHODS: Using duplex/colour Doppler sonography, the DPI was measured both before and after surgery (median, 9 months) in 14 patients undergoing apparently curative resection for colorectal cancer. Paper-8564689.
However, the potent DA agonists, apomorphine (0.05-0.33 mg/kg) and lisuride (0.02-0.08 mg/kg), and the DA and norepinephrine agonist, DPI (0.4 and 0.8 mg/kg), did not mimic the AMPH cue or did so only partially. Paper-5116266.
We found that in populations of palm keratinocytes, selected by their ability to adhere rapidly to type IV collagen, there were significantly reduced levels of desmoplakin and other major desmosome proteins. Paper-9984145.
The mean (S.D.) amount of sodium cromoglycate excreted in the urine during the first 30 min post inhalation was 38.1 (27.5), 222.3 (120.3) and 133.1 (92.2) microg following MDI, MDI+SP and DPI, respectively. Paper-9466653.
We compared online measured Kt/V(urea) and protein catabolic rate ( PCR) with single- and double-pool Daugirdas formula (DF and eDF) based Kt/V(urea) and with protein intake derived from dietary records ( DPI). Paper-10585951.
The olfactory epithelia of eight human fetuses, aged 7 to 10 weeks (fertilization age) were immunofluorescently labeled with antibodies to the five classes of intermediate-filament proteins and to desmoplakin. Paper-5500015.
These results suggest that PKC activation plays an important role in desmoplakin translocation from the cytoplasm to the plasma membrane as one of the processes of calcium-induced desmosome formation. Paper-6162775.
More recently, DCDS measurements of the Doppler perfusion index ( DPI: ratio of hepatic arterial to total liver blood flow) have been shown to be highly accurate in the detection of these occult liver metastases. Paper-1969503.
DP siRNA treated cells sent out filopodia and came in close contact with each other when plated onto matrigel; however, in most cases they failed to form tubes as compared with control endothelial cells. Paper-10500699.
The only relationship between numbers of Leydig cells and spermatogenesis appeared to be a threshold effect, in that men with fewer than 60 million Leydig cells (4 in this study) had drastically reduced DSP. Paper-5608940.
General transcription inhibitor actinomycin D (10(-5.4 )M), but not the translational inhibitor cycloheximide (10(-5 )M), significantly abolished the DSP-induced depressed contraction to norepinephrine. Paper-12617110.
CONCLUSIONS: Budesonide/ formoterol, administered via the HFA pMDI or DPI, is an effective and well-tolerated treatment for adult and adolescent patients with asthma, with both devices being therapeutically equivalent. Paper-12574197.
Analysis of the prospective relationship revealed a significant correlation with a decrease in urea clearance (due to loss of renal function) accompanied by a decrease in both PCR (r = -0.80) and DPI (r = -0.51). Paper-718784.
DSP diluted 1:25 compared to the smallest concentration that had significant effects on smooth muscle cells reduced proliferation of endothelial cells, and in dilutions of 1:4 almost all of the cells detached. Paper-4357461.
Kinetics of desmosome assembly in Madin-Darby canine kidney epithelial cells: temporal and spatial regulation of desmoplakin organization and stabilization upon cell-cell contact. I. Biochemical analysis. Paper-5883755.
Participants with SD- DPI had been characterized by speech clinicians and caregivers as having speech delay with psychosocial issues that required attention in the course of at least 1 semester of speech treatment. Paper-11472401.
This study examined how well one manufacturer's software was able to predict REARs for a DSP behind-the-ear product in 41 adults (N = 79 ears) under four conditions (two hearing loss configurations and two inputs). Paper-11025686.
OxLDL increased NAD(P)H-associated cellular fluorescence and flavoenzyme inhibitor DPI reduced basal and oxLDL-induced MTT activity, suggesting an enhancement of NAD(P)H-dependent cellular reduction potential. Paper-12767268.
Together, these data uncover a novel mechanism by which PGD(2) functions through DP to suppress type 1 and cytolytic functions of human NK cells, thus contributing to the promotion of a type 2 immune response. Paper-13396678.
In addition, activation of the NF-kappaB promoter by PMA was diminished by pretreatment with DPI, a flavoenzyme inhibitor as well as by rottlerin, suggesting a role for reactive oxygen species (ROS) as well as PKCdelta. Paper-10655193.
Indeed, the association of additional proteins, the phosphorylation state, the subcellular localization and the level of expression all contribute to modulating heterodimer activity and are all influenced by DP proteins. Paper-9986504.
Organ culture of rat mesenteric arteries in the presence of DSP (0.2 microl/ml for 24h) resulted in markedly elevated contractile responses to the Tx analog U46619, compared with the control DMSO. Paper-12714800.
Kinetics of desmosome assembly in Madin-Darby canine kidney epithelial cells: temporal and spatial regulation of desmoplakin organization and stabilization upon cell-cell contact. II. Morphological analysis. Paper-6004949.
Calmodulin antagonists such as chlorpromazine (CPZ), trifluoperazine (TFP), and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) inhibited the phosphorylation of DPI in the AChR-rich membranes. Paper-4939739.
An immunofluorescence and immunoblotting are crucial for the differential diagnosis between an erythema multiforme which is positive for anti- DP I and II antibodies and other autoimmune bullous diseases. Paper-13236753.
Few studies have focused on the function of DP in de novo capillary formation (vasculogenesis) and branching (angiogenesis) during tumorigenesis, embryonic development, cardiovascular development or wound healing. Paper-10500699.
On the other hand, calcium ionophore A23187 induced only a temporary increase in the number of desmoplakin-containing fluorescent spots in the cytoplasm and a temporary cell-cell attachment without desmosome formation. Paper-6162775.
However, like the normal cell lines, papilloma cells underwent a transition to predominantly a spinous cell population (i.e. reactive with pemphigus and desmoplakin antibody) in response to extracellular Ca2+. Paper-6247995.
The aim of this study was to assess the potential of delivering a combination antibiotic therapy, containing doxycycline and ciprofloxacin (both hydrochloride) as a dry powder ( DPI) formulation for inhalation. Paper-13085369.
The developed system consists of an eight-electrode belt worn around the thorax, an electronic unit containing analog and digital boards, and a stand-alone DSP based system with a designated software to analyze the data. Paper-12364350.
Papilloma cells cultured under conditions of low extracellular Ca2+ were comprised of three subpopulations: cells reactive only with pemphigoid anti-serum, cells reactive only with desmoplakin antibody. Paper-6247995.
This enhanced serine phosphorylation coincided with the increase in the protein solubility, suggesting that phosphorylation of desmoplakin may be a mechanism by which PKC mediates desmosome disassembly. Paper-1986931.
Recent evidence indicates that lymphatic and vascular endothelial cells express desmoplakin, a cytoplasmic desmosomal protein that attaches intermediate filaments to the plasma membrane in epithelial cells. Paper-1655732.
The use of DPB and DPI as radiation modifiers allows the elimination of undesirable side reactions of aryl radicals and through hydrogen abstraction results in high yields of a species identified as the DNA C1'. sugar radical. Paper-1418397.
Dual, activatory and inhibitory, effects of a cardiotonic drug DPI 201-106 on Na+ channels were compared with the redox properties of the DPI itself and of the constituents of its molecule, indole and piperazine. Paper-1111110.
The discovery of the potent and selective prostaglandin D2 (PGD2) receptor ( DP) antagonist [(3R)-4-(4-chlorobenzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4-tetrahydrocyclopenta[b]indol-3-yl]-acetic acid (13) is presented. Paper-13111925.
Daily spermatozoan production per testis ( DSP/T) and numbers of epididymal spermatozoa were determined from counts of maturation-phase spermatids or epididymal spermatozoa in tissue homogenized in a Waring blender. Paper-5999821.
Desmosomes have a characteristic staining pattern that is easy to interpret, and monoclonal antibodies to desmoplakin may prove useful as markers for distinguishing undifferentiated carcinomas from nonepithelial cancers. Paper-5872162.
This 2-week, multi-center (34 sites), randomized, modified-blind, parallel group study evaluated the efficacy and safety of concomitant treatment with nebulized arformoterol (the formoterol(R,R)-isomer) BID and tiotropium DPI QD. Paper-13642984.
The mean diameter of the particles produced and the crystallinity of phenytoin in the drug/polymer particles were evaluated with an Aerosizer DSP Particle Size Analyzer and powder X-ray diffraction, respectively. Paper-11392020.
In NHEK cells grown at Ca2+ concentrations of 0.11 mM, desmoplakin appears to be localized in electron-dense bodies associated with intermediate filaments at sites of cell-cell contact in the absence of formed desmosomes. Paper-6496074.
BACKGROUND: Besides being present in paraneoplastic pemphigus (PNP), circulating antidesmoplakin ( DP) antibodies have been found anecdotally in other bullous diseases, including pemphigus foliaceus and pemphigus vulgaris. Paper-11386338.
CONCLUSIONS: We have described a new dominant mutation in desmoplakin that causes left-sided ARVC, with arrhythmias of LV origin, lateral T-wave inversion, and late gadolinium enhancement in the LV on magnetic resonance images. Paper-11300468.
A novel luminol-coated target cell chemiluminescence assay is described in which luminol is conjugated to the target cell surface with the bifunctional crosslinker 3,3'-dithiobis(propionic acid N-hydroxysuccinimide ester) ( DSP). Paper-5354175.
In contrast, a leftward shift was present in the force-calcium relationship in the presence of DPI in aequorin-loaded and skinned muscles from myopathic hearts, indicating an increase in the sensitivity of the myofilaments to calcium. Paper-5976890.
However, the ratio of the hepatic arterial flow to the sum of the portal and hepatic arterial flows (Doppler perfusion index, DPI) was markedly elevated (P < 0.0001) in the patients with metastases when compared with the controls. Paper-7463580.
The MGBs are analogs of the potent antibiotic CC-1065 and consist of repeating 1,2-dihydro-3H-pyrrolo[3,2-e]indole-7-carboxylate ( DPI) subunits with N-3 carbamoyl or tert-butyloxycarbonyl groups (CDPI or BocDPI subunits, respectively). Paper-412257.
Taken together, these results clearly demonstrate an important role for PKC in the regulation ofdesmosomal junctions in HeLa cells, and identify serine phosphorylation of desmoplakin as a crucial event in this pathway. Paper-1986931.
Although differences were observed when comparing the total volume of the upper airway during inhalation via the DPI (70 [17] cm3) to the pMDI (56 [20] cm3, p = 0.037) or spacer (59 [12] cm3, p = 0.022), these did not reach significance. Paper-10877837.
BACKGROUND AND PURPOSE: Echo-planar diffusion-weighted imaging (DWI) and delayed postcontrast T1-weighted MR imaging ( DPI) have been proposed in previous studies to detect residual middle ear cholesteatomas, with varying results. Paper-12908640.
Desmoplakin, a constitutive component of the desmosomal plaque, is the most abundant protein present in such junctions and plays a critical role in linking the intermediate filament network to the plasma membrane in these tissues. Paper-1757516.
In 12 autopsy cases representing a 100-fold range of sperm production rates, the average difference between DSP/gm values obtained from simulated biopsy specimens and from entire testes was not significantly different from zero (P < 0.05). Paper-3578818.
RESULTS: The three formoterol formulations demonstrated significant (P < or = 0.05) improvements in bronchoprotection compared to placebo and non-inferiority of the HFA preparation compared to the CFC and DPI preparations was demonstrated. Paper-11414567.
These aAbs did not only bind to desmosomal plaques of epithelial cells where they colocalized with defined murine monoclonal antibodies directed against desmoplakin I and II, but also labeled the intercalated discs of myocardial cells. Paper-146373.
CONCLUSION: Our findings of similar efficacy, pharmacokinetics and systemic effects of the HFA formoterol inhaler compared to the CFC and DPI preparations supports the potential use of this novel formulation in the treatment of asthma. Paper-11414567.
AIM: In patients undergoing apparently curative resection for colorectal cancer, an elevated Doppler Perfusion Index ( DPI; ratio of hepatic arterial to total liver blood flow) before surgery is associated with a high risk of recurrence. Paper-8564689.
These data led us to suggest that in DP IV and related peptidases ligand and antibody binding sites are non-linear and that enzyme activity depends on charged sidechains that surround the entrance to the central tunnel of the beta-propeller. Paper-8656037.
Rabbit aortic smooth muscle cells were exposed to hypoxia and the DMSO-soluble particulate matter of cigarette smoke ( DSP) and the effects on the cells were compared to those of ultraviolet (UV) light, a wellknown cytotoxic stimulus. Paper-4357461.
Both beta- and gamma-catenins are reported to link classical cadherins to the actin cytoskeleton, but only gamma-catenin binds to the desmosomal cadherins, which links them to intermediate filaments through its association with desmoplakin. Paper-12870956.
RESULTS: We identified in the patient a homozygous missense mutation in exon 24 of the desmoplakin gene, leading to a Gly2375Arg substitution in the C-terminal of the protein where the binding site to intermediate filaments is located. Paper-9783674.
Lymphatic vessels were identified by using a cocktail of IgGs for desmoplakin I and II while the presence of inflammatory cytokines was determined by the expression of major histocompatibility complex (MHC) class II in the venules. Paper-1775888.
In experimental systems devoid of cell walls ( protoplasts and membrane fractions) superoxide anion (O(2)(-)) production was inhibited by 50 and 100 mM NaCl, 50 microM DPI, 10 mM EGTA, and 5mM verapamil, a Ca(2+) channel inhibitor. Paper-13101680.
Preliminary genotype-phenotype assessment indicates that mutations affecting the outer dense plaque of desmosome (desmoglein2, plakoglobin, plakophilin2 and the N-terminal of desmoplakin) result in ARVC with the ordinary described phenotype. Paper-12316174.
IMD-0354 (10(-7.5 )M), a specific nuclear factor-kappaB (NF-kappaB) pathway inhibitor, markedly reversed the DSP-induced down-regulation of alpha(1A)-adrenoceptor expression in the smooth muscle at both functional and mRNA levels. Paper-12617110.
Furthermore, the systemic bioavailability (adjusted to correspond to the same drug dose as that of the comparator product deposited in the lung) was found to be 1.6 times higher for the comparator product Tobi((R)) than for the two DPI formulations. Paper-12688184.
In the case of K8/K18, however, the interaction as assessed by yeast two-hybrid assays increased 9-fold when a serine located in a protein kinase A consensus phosphorylation site 23 residues from the end of DP was altered to a glycine. Paper-1135127.
The similarity in number of spermatozoa in epididymides of men whose DSP/T differed by threefold is consistent with the inability of the human epididymis to store many spermatozoa when no blockage is present.(ABSTRACT TRUNCATED AT 250 WORDS) Paper-5999821.
The uncoupled electron transport from photosystem II (PSII) (water to 2,6-dichlorophenol-indophenol ( DPIP)) and photosystem I (PSI) (2,6-dichlorophenol-indophenol reduced (DPIPred) to MV) was inhibited with 500 microM of 5d by 22 and 14% respectively. Paper-11316306.
When isolated frog ( Rana catesbeiana) rod outer segment (ROS) fragments were incubated with [gamma-32P]ATP in the dark, only two of phospholipids, i.e., phosphatidylinositol-4-phosphate ( DPI) and phosphatidic acid (PA) incorporated 32P. Paper-5115085.
In order to determine this we prepared a number of stratified squamous epithelial tissues for indirect immunofluorescence using antibody preparations directed against known desmosome components including desmoplakin and certain glycoproteins. Paper-5197543.
A total of 74 human embryos were stained with gap junction protein specific anti-peptide antibodies an antibodies to the desmosomal protein desmoplakin to reveal the expression pattern of intercellular junctions during preimplantation development. Paper-1141885.
The therapeutic efficacy of dry powder formulations PM(2), PM(10) of IL-1Ra was studied at nonforced inhalation in an aerosol chamber using the DPI " Spinhaler". Micronized powder formulations for insufflation were produced by air-jet milling. Paper-12652357.
Furthermore, thrombin (Trap6)-induced platelet aggregation and thrombus formation on collagen under high shear was inhibited by NAD(P)H oxidase inhibitors ( DPI and apocynin), whereas secretion and platelet shape change were not affected. Paper-11824467.
DPI increased Vmax for side chain cleavage in both DMPC and DOPC vesicles to the same rate (12 min-1) without effect on the Km for cholesterol, while cardiolipin both produced a similar increase in Vmax and decreased Km ( cholesterol). Paper-4460335.
A randomized, double-blind, double-dummy protocol was used to compare the safety and efficacy of beclomethasone dipropionate (BDP) delivered by a novel dry powder inhaler ( DPI, Clickhaler) or by a pressurized metered-dose inhaler (MDI) plus spacer. Paper-8496587.
This effect was completely inhibited by coincubation of IMAs with DPI (100 micromol/L), a nonspecific Ang II antagonist ([sar(1), thre(8)]-Ang II, 1 micromol/L) and a specific Ang II type 1 (AT(1)) receptor antagonist ( losartan, 1 micromol/L). Paper-8492540.
MAIN OUTCOME MEASURES: Subsequent pregnancies; school completion; children's development, indicated by the Developmental Profile II ( DPII); parental attitudes, indicated by the Adult-Adolescent Parenting Inventory (AAPI); and maternal perceptions. Paper-469249.
This study compared the efficacy and safety of a novel hydrofluoroalkane (HFA) pressurised metered-dose inhaler (pMDI) formulation of budesonide/ formoterol with that of budesonide pMDI and budesonide/ formoterol dry-powder inhaler ( DPI; Turbuhaler). Paper-12574197.
Analyses of the conversational speech samples indicated that in comparison with participants in the control group, those with SD- DPI had significantly more severe speech delay, averaging approximately 7% to 10% lowered speech competence in conversation. Paper-11472401.
The patterns of cytoskeletal differentiation were studied in 20 testicular non-seminomatous germ cell tumors by immunohistochemistry, using diverse monoclonal antibodies specific for different intermediate filament (IF) proteins and for desmoplakin. Paper-6875792.
Organ culture of the arterial segments in the presence of DSP (0.2 microl/ml) resulted in a significantly decreased contractile response to norepinephrine, compared to control (i.e. in the presence of dimethyl sulfoxide) (P < 0.05). Paper-12617110.
On three separate randomised study days, 7 days apart, subjects inhaled (i) 4x5 mg from an Intal metered dose inhaler (MDI), (ii) 4x5 mg from an MDI attached to a large volume spacer (MDI+SP) and (iii) 20 mg from an Intal Spinhaler ( DPI). Paper-9466653.
Doppler Perfusion Index, ( DPI, ratio of hepatic arterial to total liver blood flow) and Doppler Flow Ratio (DFR, ratio of hepatic arterial to portal venous blood flow) of controls and patients with overt liver metastases were clearly separated (p < 0.0001). Paper-7766952.
In basal cell carcinoma cells desmoplakin is not restricted to desmosomes but also occurs in certain electron-dense bodies morphologically similar to those seen in NHEK maintained in low levels of Ca2+ and during early stages of desmosome assembly. Paper-6496074.
T2DR serum enhanced E-selectin expression in a ROS-dependent manner since this process was significantly attenuated not only by tiron (1 mM), a superoxide scavenger, but also by DPI (10 micromol/L) and apocynin (100 micromol/L), inhibitors of NAD(P)H oxidase. Paper-11840529.
Four different microemulsions differing in their core lipid component ( triolein, canola oil, squalene, or seal oil) and containing 1,3-dihydroxypropan-2-one 1,3-diiopanoate ( DPIP), a potential radioimaging probe, were prepared by means of ultrasonication. Paper-1909095.
By double indirect immunofluorescence the desmoplakin antiserum stains a double fluorescent line along the contacting edges of cultured keratinocytes, whereas the positive pemphigus serum samples stain a single fluorescent line along this same border. Paper-5167711.
There were no significant differences in morning peak expiratory flow (PEF) (primary endpoint), evening PEF, overall daytime or nighttime symptom scores, or lung function parameters ( forced expiratory volume in 1 sec, forced vital capacity) between DPI and MDI. Paper-8496587.
These results indicate that DP is required for anchoring IF networks to desmosomes and furthermore suggest that the DP-IF complex is important for governing the normal spatial segregation of adhesive junction components during their assembly into distinct structures. Paper-645581.
This hypothesis has been tested by analyzing gap junctions in human cardiomyopathies caused by mutations in plakoglobin and desmoplakin, intracellular proteins that link adhesion molecules at cell-cell junctions to the cardiac myocyte cytoskeleton. Paper-11304325.
Computer-aided analysis of the DPI-predicted amino acid sequence indicates that the central domain, which contains the heptad repeat characteristic of many alpha-fibrous proteins, will participate in the formation of a coiled coil dimer approximately 130 nm in length. Paper-6576149.
CONCLUSION: Although antibodies to desmoplakin I and II have previously been associated with paraneoplastic pemphigus, their occasional finding in erythema multiforme and bullous pemphigoid indicates that they can also occur in non-neoplastic pemphigus. Paper-1648949.
The aim of this present paper was to determine the immunohistochemical (IHC) expression of DP in breast cancer specimens (n = 75) in comparison with ductal carcinoma in situ ( DCIS) (n = 26), tumour associated normal (n = 29) and normal breast tissue (n = 7). Paper-8275391.
A slow MyHC-related antigen is punctate on the cell surface and co-localizes with desmoplakin at desmosomal junctions of all suprabasal epidermal layers from rat foetal day 16 onwards, in the root sheath of the hair follicle and in intercalated disks of cardiomyocytes. Paper-10188696.
In functional tests, the total ACh-induced dilatation, the nitric oxide (NO)-mediated and the endothelium-derived hyperpolarization factor (EDHF)-mediated dilatations were significantly decreased by DSP in a time- and concentration-dependent manner (p < 0.05). Paper-10808457.
LPC-induced adhesion was significantly reduced when EC were co-incubated with IBX, or NAD(P)H oxidase inhibitor diphenyleneiodonium ( DPI, 5 microM) and also blocked by addition of 2-aminoethoxydiphenylborate (2-APB, 100 microM) or the calcium-chelator BAPTA (10 microM). Paper-12377390.
Corresponding to the finding of desmosomes in ultrathin sections, focal collections of intramembranous particles were observed in freeze-fracture replicas and a positive immunohistochemical reaction for desmoplakin in the sinuses was found at the light microscopic level. Paper-6440713.
OBSERVATION: A 61-year-old woman with pemphigus foliaceous by clinical, histological and immunofluorescent criteria was found to have circulating antibodies to 250 and 215 kDa antigens identified as desmoplakin I and II by immunoblotting and immunoprecipitation. Paper-1648949.
CONCLUSIONS: This study demonstrated the significance of interacting influences by variables commonly encountered in the use DPI such as variation in patient's inspiratory flow rate, ambient RH and repeated inhaler use on the electrostatic behavior of a lactose DPI carrier. Paper-13479062.
Mutations in genes encoding desmoplakin and plakoglobin suggest that altered integrity at cardiac myocyte cell-cell junctions may promote myocyte degeneration and death, with the repair process consisting of replacement of myocardium by adipose and fibrous tissue. Paper-9951564.
Tissue sections of mouse skin processed for double indirect immunofluorescence using each of the pemphigus serum samples and a rabbit antiserum directed against a component of the desmosomal plaque ( desmoplakin) show similar punctate cell surface staining patterns. Paper-5167711.
Immunoprecipitation experiments and immunoblotting with anti-phosphoserine, antiphosphothreonine and anti-phosphotyrosine antibodies revealed that desmoplakin was mainly phosphorylated on serine and tyrosine residues in both treated and untreated cells. Paper-1986931.
The protonation-state determinations were not above 2sigma (the best was 1.81sigma) and therefore an extrapolation is given, based on 100% data completeness and the average DPI, that a 3sigma determination could be possible if X-ray data could be measured to 1.02 A resolution. Paper-12836454.
The properties of a novel disulfide-bond-containing gemini surfactant bis[N,N-dimethyl-N-hexadecyl-N-(2-mercaptoethyl)ammonium bromide] disulfide ( DSP) were studied using a Langmuir balance, supported monolayers, differential scanning calorimetry, giant vesicles, and LUVs. Paper-11341581.
In this study we used well-characterized antibody against desmoplakin I and II immunohistochemically and immunoelectron microscopically on two cases of pemphigus vulgaris and one case of pemphigus erythematosus and two cases each of Hailey-Hailey's disease and Darier's disease. Paper-35303.
A bacterial strain DSP capable of utilizing chlorpyrifos as a sole source of carbon and energy was isolated 21 days after the third chlorpyrifos application, which indicated that the capability of soil microorganism for degrading chlorpyrifos was formed during the experiment. Paper-12999922.
When these men were sorted by increasing numbers of Leydig cells per man into two, three, or five groups, no difference could be found between or within these groups when daily sperm production per man ( DSP); seminiferous tubular volume, diameter, or length; or seminiferous epithelial volume was examined. Paper-5608940.
The objective of this study was to make nano-salbutamol sulphate (SBS) DPI, radiolabel it with Tc-99m using a novel surface labeling methodology, characterize the formulation and assess its in vitro and in vivo deposition in healthy human volunteers to estimate its bioavailability in the target area. Paper-13616655.
OBJECTIVES: The aim of this study was to compare the bronchoprotective and bronchodilator effects of a single dose of 12 microg of formoterol from the HFA Modulite inhaler with the Foradil Aerolizer (dry powder inhaler, DPI) and the Foradil CFC inhalers (Novartis Health Consumer, Basel, Switzerland). Paper-11414567.
With visual-auditory biofeedback as a cue for heel contact and toe push-off condition during treadmill ambulation, the improvement of four dynamic gait performance measures (Double Support Period, DSP, Constant Time Cadence, CTC, Single Support Period, SSP, and Stance/Swing Ratio, SSR) in amputees was verified. Paper-12627481.
Here we report, for the first time, that clusters of basal keratinocytes at the tips of the rete ridges in human palm, previously recognised as a major repository of stem cells, had very low levels of desmoplakin protein and mRNA expression, compared with cells at the sides of the ridges or above the dermal papillae. Paper-9984145.
On three DPI delivered images, namely relative regional cerebral blood volume (rrCBV), uncorrected mean transit time (MTTu) and CBF index images, correlations between initial lesion volume of and follow up infarction volume of three images and rCBF images delivered with singular value decomposition (SVD) methods were assessed. Paper-10097985.
OBJECTIVE: We sought to evaluate step-down therapy with a fluticasone propionate-salmeterol (FP-SM) combination administered through a dry powder inhaler ( DPI; Advair Diskus) versus a medium dose of hydrofluoroalkane 143a-beclomethasone dipropionate (HFA-BDP) administered through a breath-actuated pressurized metered-dose inhaler (QVAR Autohaler). Paper-9212467.
In this issue of the JCI, Garcia-Gras et al. demonstrate that cardiac-specific loss of the desmosomal protein desmoplakin is sufficient to cause nuclear translocation of plakoglobin, upregulation of adipogenic genes in vitro, and a shift from a cardiomyocyte to an adipocyte cell fate in vivo (see the related article beginning on page 2012). Paper-12139151.
Previous authors using immunolocalization of desmoplakin, a marker protein for the desmosomal plaque, in LCM-grown cells have interpreted positively stained, dense, cytoplasmic aggregates on intermediate filaments (IF) bundles as preformed plaque units which upon NCM shift would move to the plasma membrane and contribute to desmosome formation. Paper-6089594.
PURPOSE: To assess the value of the Doppler perfusion index ( DPI = the ratio of hepatic arterial to total liver blood flow) as measured with duplex and color Doppler sonography (DCDS) in comparison with computed tomography (CT), conventional ultrasound (US), and laparotomy for the detection of liver metastases in patients with colorectal cancer. Paper-180461.
In addition to the recently reported effectiveness of N-ethylmaleimide, nordihydroguaiaretic acid, and trifluoperazine the agents diacylglycerol kinase inhibitor II, emodin, D609, DPI, KT5720, KT5926, MK-886, bisindolylmaleimide I, and (+/-)methoxyverapamil were able to reduce aggregate stability in the presence of the haptenic sugar. Paper-2012314.
These synonyms are used for gene DSP (desmoplakin): PPKS2, KPPS2, DPII, DPI, DP, Desmoplakin, 250/210 kDa paraneoplastic pemphigus antigen.
These accession numbers are used for gene DSP: Q4LE79 (UNIPROT__AC), O75993 (UNIPROT__AC), AAF19785 (NCBI_GENBANK__AC), AAA85135 (NCBI_GENBANK__AC).
DSP is a homologue of DSP (desmoplakin) from Bos taurus.
DSP is a homologue of DSP (desmoplakin) from Pan troglodytes.
DSP is a homologue of DSP (desmoplakin) from Gallus gallus.
DSP is a homologue of Dsp (desmoplakin) from Mus musculus.
DSP is a homologue of Dsp (desmoplakin) from Rattus norvegicus.
Important links !
iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.