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Activation of coagulation factor X in human semen. Paper-1514721.
Severe sepsis results in an elevation of F10/ 50 only. Paper-851603.
Of the three peptides, only F10 was found in the hemolymph. Paper-1250802.
Three murine melanomas (F1, F10, B16) were studied. Paper-4000241.
This suggests a broad role for FX in adenovirus infectivity. Paper-13153086.
Congenital factor X (Stuart-Prower factor) deficiency: a family study. Paper-6016878.
Oral contraceptives caused increased fibrinogen, FVII, FX, and fibrinolysis. Paper-7385427.
Kinetics of coagulation factor X activation by platelet-bound factor IXa. Paper-6538645.
SCE was encountered in 29 and 13% of second division metaphases of BL6 and F10. Paper-6112413.
FX genes of the affected family members were analyzed by sequence analysis. Paper-12478647.
The cumulus cell monolayer was prepared in follicular fluid (FC) and Ham's F10 (HC). Paper-10987159.
Coagulation factor X also binds to Mac-1 on monocytes at a similar site to C3bi. Paper-126851.
Activated coagulation factor X: a novel mitogenic stimulus for human mesangial cells. Paper-8795174.
The structural gene for human coagulation factor X is located on chromosome 13q34. Paper-4927783.
In addition, ixolaris interacts with FX possibly through the heparin-binding proexosite. Paper-10732673.
Improved human zygote development in a modified Ham's F10 medium in vitro. Paper-859757.
Prostacyclin stimulation of the activation of blood coagulation factor X by platelets. Paper-5392876.
Impaired human embryo development in Earle's culture medium compared to Ham's F10. Paper-7425232.
LGMM did not appear to improve the pregnancy outcome compared to normal Ham's F10. Paper-859757.
The proband is the first reported case of homozygote for the FX Gly366Ser mutation. Paper-11239410.
The number of SCEs per chromosome was twice as high in F10, BL6 and BL6-ML8 as in the F1 cells. Paper-6112413.
Cloning and expression in COS-1 cells of a full-length cDNA encoding human coagulation factor X. Paper-31206.
An aliquot was also incubated for 60 min and Ham's F10 medium containing 50 USP/ml heparin. Paper-1701958.
Intracranial hemorrhage (ICH) is a severe complication of Factor X ( FX) deficiency. Paper-11187208.
More than 80 mutations of the F10 gene have been identified, most of which are missense mutations. Paper-13548717.
The treatment groups were as follows: normal Hams F10, MM, and MM with 0.5 mM glucose (LGMM). Paper-859757.
Evidence for an essential catalytic role of the F10 protein kinase in vaccinia virus morphogenesis. Paper-10208119.
Differences between human and rabbit coagulation factor X-implications for in vivo models of thrombosis. Paper-9603336.
Acquired, transient factor X ( Stuart factor) deficiency in patient with mycoplasma pneumonial infection. Paper-3411484.
The effect of calcium (II) on the binding of anticoagulation factor I with activated coagulation factor X. Paper-8786126.
In this system, BL6 cells could transfer dye into endothelial cells but F10 cells could not. Paper-8488846.
The coagulation factor X activator from Russell's viper venom (RVV-X) is a heterotrimeric glycoprotein. Paper-12908875.
These FX variants should be useful for site-specific induction of blood coagulation in the tumor vasculature. Paper-11542479.
Classical hemophilia results from a defect of the intrinsic tenase complex, the main factor X ( FX) activator. Paper-11153360.
The patients with FII, FVII or FX defects had a total of 14 pregnancies and often needed transfusion therapy. Paper-10810412.
When supplemented with protein, Ham's F10 was the poorest of the media in supporting embryo development. Paper-6688531.
Washed human healthy sperm were suspended in Ham's F10 and exposed to varying concentrations of curcumin. Paper-10102199.
Finally, we were able to define a linear epitope recognized by the Ul/ F10 antibody on the nucleocapsid protein. Paper-122123.
A flow cytometric method for determining the binding of coagulation factor X to monocytes in whole human blood. Paper-10658816.
Russel viper venom directly activates FX, in presence of PF3, is an indicator of common pathway of coagulation. Paper-13391054.
Pharmacokinetics and pharmacodynamics of the dual FII/ FX inhibitor BIBT 986 in endotoxin-induced coagulation. Paper-13242229.
Inactivation of human blood coagulation factor X by chemical modification of gamma-carboxyglutamic acid residues. Paper-4661326.
Renewing the medium (Ham's F10 with 30% serum) at 7 h resulted in better sperm motility and velocity at 22 h. Paper-5320636.
Also after 24 h, the percentage of spermatozoa which were highly motile was greater in HGMM than in Ham's F10. Paper-970858.
F10 MAb was obtained using cell membrane components from a trophoblast cell line HT as immunogen. Paper-490454.
In this study we investigated anti-tumour properties of angioarrestin in B16 ( F10) melanoma tumour model. Paper-11463814.
F9 and F10 also cross-reacted with the antisera against ascariasis and F15, with antisera against angiostrongylosis. Paper-1558803.
Herpes simplex virus type 1-encoded glycoprotein C contributes to direct coagulation factor X-virus binding. Paper-10842329.
However, motility rapidly decreased (P < 0.05) with the addition of NaHCO3 to PBS or Ham's F10 nutrient mixture. Paper-884175.
Gel-filtration chromatography demonstrated 1:1 complex formation between fluorescein-labeled Ixolaris and FX. Paper-12669683.
These studies provide evidence for an essential catalytic role of the F10 kinase in vaccinia virus morphogenesis. Paper-10208119.
Blood coagulation Factor X is a serine protease required for both the intrinsic and extrinsic pathways of coagulation. Paper-65900.
Stuart Prower factor (Factor X) deficiency is a rare hereditary autosomal recessive coagulation disorder. Paper-11107412.
Furthermore, cellular oxidation of LDL could be partially mimicked by the addition of cysteine to Hams F10 medium. Paper-848480.
The internalization and efflux of (111)In-DOTA-GlyGlu-CycMSH were examined in B16/ F10 melanoma cells. Paper-13687050.
Several international registries of FX-deficient patients have greatly expanded the knowledge of clinical phenotype. Paper-13548717.
In all three cases, the hybridization pattern F10 - G9 - PT1526 indicates a centromere to telomere orientation. Paper-8852302.
We assessed the vaccine for its prophylactic and therapeutic effect against the murine B16 F10 melanoma. Paper-9386795.
Blood coagulation Factor X and its activated form Factor Xa play an essential role in the midphase of the clotting cascade. Paper-477709.
Furthermore, cellular oxidation of LDL could be partially mimicked by the addition of cysteine to Hams F10 medium. Paper-8053743.
The leukocyte beta2-integrins have several functions, including serving as receptors for coagulation factor X and fibrinogen. Paper-1662108.
Identification of the oligosaccharide structures of human coagulation factor X activation peptide at each glycosylation site. Paper-300604.
FX activity was markedly reduced (FX:C <1%) in prothrombin time and aPTT assays, and was 15% of normal in the RVV-X assay. Paper-13733858.
Complex media (RPMI 1640 and Ham's F10) generally caused a greater progesterone release than simple salt solution (EBSS). Paper-7074611.
Liver infection by the FX-Ad5 complex is mediated through a heparin-binding exosite in the FX serine protease domain. Paper-12736621.
Activation of coagulation factor X via the intrinsic pathway requires the assembly of factors IXa and VIII on lipid membranes. Paper-6952195.
There was a significant association between serum triglyceride levels with FVII and FX, but not with the ACE I/D genotype. Paper-9937065.
In this study we explored the use of human maternal serum (HMS) from early gestation as the medium supplement to Ham's F10. Paper-7867207.
The highly metastatic line, B16 F10, forms less prostaglandin D2 compared to the moderately metastatic parent line, B16 F1. Paper-3334758.
Antibody phage display was employed to isolate two scFv antibody fragments, D8 and F10, with specificity for the VEGF165 isoform. Paper-9790137.
With husband insemination, 264 Testyolk and 253 Ham's F10 cycles had pregnancy rates of 37% and 35%, respectively (P = .23). Paper-7639162.
Directed glycosylation of human coagulation factor X at residue 333. Insight into factor Va-dependent prothrombin catalysis. Paper-8640272.
No predominant structure was found in the chain segment from Ala(1) to Gly(6) for F10 in both H2O and dimethyl sulfoxide. Paper-6082163.
Unlike 4-HPR, NONO-AM induced apoptosis in a dose-dependent manner in both parental MDA-MB-231 cells and F10 cells. Paper-10823014.
Many host molecules, including coagulation factor X, have been identified and suggested to serve as mediators of Ad liver tropism. Paper-13740375.
Tissue factor (TF)-bound factor (F)VIIa plays a critical role in activating FX, an event that rapidly results in blood coagulation. Paper-10084627.
F10 antibody reacted with an ATLV structural polypeptide ( gp21 ) with a m.w. of 21,000 that was glycosylated on cell surfaces. Paper-4700661.
In situ hybridization was used to assign F10 to region 13q32----qter of chromosomes from normal human lymphocytes. Paper-5249336.
A number of studies suggest that blood-clotting factor X ( FX) uses secondary site(s) to interact (as a substrate) with its activators. Paper-10328116.
The construct was transfected into dexamethasone receptor negative F1 and receptor positive F10 cells of the B16 murine melanoma. Paper-138567.
Treatment of mice with rHuIFN-alphaA/D following B16 F10 injection resulted in no significant inhibition of pulmonary metastases. Paper-4646369.
During blood coagulation, factor IXa (FIXa) activates factor X ( FX) requiring Ca2+, phospholipid, and factor VIIIa (FVIIIa). Paper-10980781.
The association of the ACE D allele with lower activities of FVII and FX was also seen in patients with coronary artery disease (CAD). Paper-9937065.
Human FX substantially enhanced hepatocyte transduction by CAR-permissive and mutated viruses in an ex vivo liver perfusion model. Paper-12256137.
The highly metastatic F10 subline of the B16 melanoma was maintained in tissue culture to assure high NK sensitivity of the cells. Paper-4627736.
We investigated the antitumor effects of human recombinant interleukin-6 (hrIL-6) on the highly metastatic B16 melanoma clone F10. Paper-7866184.
Fifty microg/ml LDL protein was incubated with macrophages in Ham's F10 medium, supplemented with additional Fe2+, for up to 48 h. Paper-1871722.
Two cases of Hansen disease demonstrating a lupus-like inhibitor directed against the activation of coagulation factor X are described. Paper-3596091.
The bone metastatic F10 cells were more sensitive to the anti-invasive effects of 4-HPR and NONO-AM than were MDA-MB-231 cells. Paper-10823014.
Activation of blood coagulation factor X by arginine-specific cysteine proteinases (gingipain-Rs) from Porphyromonas gingivalis. Paper-1065918.
The gene coding for coagulation factor X was studied in a family segregating chromosomal abnormalities involving chromosomes 13 and 6. Paper-4927783.
Oocytes classified as mature were cultured for 0, 2, 4, 6, or 8 h in Ham's F10 medium and then stained for nuclear maturation. Paper-8145596.
The embryos of the C57BL X CBA strain showed the highest hatching rate in BWW but the expanded blastocyst rate was highest in F10. Paper-5952717.
All patients were homozygous for a novel FX mutation (Gly381Asp) in the structurally conserved region of the serine protease active site. Paper-9828314.
P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Paper-3683145.
Measurement of the kinetics of inhibition of activated coagulation factor X in human plasma: the effect of plasma and inhibitor concentration. Paper-5300016.
With donor sperm, 229 cycles with Testyolk and 314 cycles with Ham's F10 had cumulative pregnancy rates of 68% and 48%, respectively (P = .52). Paper-7639162.
Fertilization rates were significantly lower in oocytes cultured in MM (23.8%) compared to LGMM (75.5%), HGMM (73.6%) or Ham's F10 (71.1%). Paper-970858.
In the sepsis patient group, a significantly higher F10/50 was measured (52% +/- 3% severe sepsis vs 40% +/- 1% control subjects, p < .01). Paper-851603.
Upon complex formation with its cofactor FVIIIa and Ca2+-mediated binding to phospholipid membranes, FIXa becomes a very potent activator of FX. Paper-1125614.
The F10 gene encodes a 52-kDa serine/ threonine protein kinase (protein kinase 2), an essential protein involved in virus morphogenesis. Paper-876804.
Blastocyst formation was lower (P < 0.05) on oviductal cells than in mKrb-Ham's F10 treatment and was < 20% in all treatments. Paper-742726.
The potential link between ACE I/D polymorphism and the plasma activities of FVII and FX is probably independent of triglyceride metabolism. Paper-9937065.
Family studies were performed in order to distinguish the contributions of individual mutant F10 alleles to the clinical and laboratory phenotypes. Paper-2164537.
RESULTS: The patient we studied was homozygous for a substitution of arginine for serine at codon -30 in the signal sequence of F10. Paper-11525610.
Anticoagulant and antithrombotic properties of a gamma-carboxyglutamic acid-rich peptide derived from the light chain of blood coagulation factor X. Paper-5333931.
Penetrance and expressivity of the gene for double podding was studied in an F2 population and F10 recombinant inbred lines (RILs) of this cross. Paper-8367605.
Antibodies against the Activated Coagulation Factor X (FXa) in the Antiphospholipid Syndrome That Interfere with the FXa Inactivation by Antithrombin. Paper-12322459.
Searching the literature, we discovered only 9 additional patients with prothrombin deficiency or FX deficiency, having a total of 16 pregnancies. Paper-10810412.
F10 remained associated with the particulate core fraction of mature virions after treatment with a nonionic detergent and reducing agent. Paper-10208120.
LDL, preincubated with 5 microM copper or with macrophages in Ham's F10 medium, accumulated in macrophages much more than did native LDL. Paper-7951999.
This integral membrane glycoprotein forms a ternary complex with factor VIIa (FVIIa) and zymogen factor ( FX), which is then activated to factor Xa (FXa). Paper-12620406.
It was found that thymidine did not increase the cytotoxicity of BCNU for B16/ F10 melanoma or L1210 leukemia cells in vitro. Paper-1414215.
The identity of the insert in a selected phage lambda clone was confirmed to be FX by nucleotide (nt) sequence analysis and restriction mapping. Paper-31206.
We generated an amphotropic retroviral vector with the human FX cDNA and delivered it to rat hepatocytes in vivo during liver regeneration. Paper-905791.
In addition, sperm motility in fresh sperm was observed in samples incubated in Testyolk or Ham's F10, with motility counts performed at 0, 6 and 24 hours. Paper-7639162.
The bleeding patterns of the enrolled patients showed differences that are associated with the types of F10 mutation, and the corresponding genotypes. Paper-12184305.
The lowest concentrations capable of 100% kill after exposure for 1 min were 0.1 ml l(-1) for TriGene, 0.33 ml l(-1) for F10 and 100 ml l(-1) for Betadine. Paper-13177974.
The NTC-121 cells were injected intracranially in C57BL/6 mice (N = 10/group) along with non-irradiated, non-transfected B16/ F10 (wild type) melanoma cells. Paper-9963979.
We began our research by elaborating a method for the construction of subtracted cDNA libraries, and made it applicable to BL6 and F10 cells. Paper-9603441.
Inhibition of the tissue factor-factor VII mediated activation of coagulation factor X by a monoclonal IgM antibody expressing lupus anticoagulant activity. Paper-6539887.
Spermatozoa from 15 fertile men were washed with Ham's F10 and incubated with two commercially available sperm nutrient media for 2, 4, 6, and 24 h. Paper-5965917.
The effect of Platinum (II) complexes with mercaptopyridines on cell lines ( fibroblasts 3T3 and the tumour ones F10, Föhn, Lovo) were studied. Paper-7814376.
Semen was collected in 15 mL medium (Ham's F10 [Gibco, Grand Island, NY] + 10% whole blood serum) and prepared with the wash and swim-up method. Paper-6619104.
After chorionic villus biopsy of human placenta, cell cultures were propagated with Ham's F10 medium or Eagle's minimum essential medium (MEM). Paper-262757.
The activation of human blood coagulation factor X on the surface of endothelial cells: a comparison with various vascular cells, platelets and monocytes. Paper-140067.
Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid. Paper-4460677.
The transferred gene was switched on in two transfectant clones of F10, by exposure to 10(-6) M dexamethasone, but not in clones of the receptor negative F1 line. Paper-138567.
Procoagulant activity was demonstrated to activate prothrombin in rat uterus, to activate human coagulation factor X, and to cause clot formation by human plasma. Paper-6595165.
FX derivatives were constructed in which point mutations were made or parts of h163-170 were substituted with the corresponding region of either FVIIa or FIXa. Paper-12527883.
Several metabolic consequences of the synergistic interaction between ara-C and guanosine on cell growth were determined in B16 F10 mouse melanoma cells. Paper-7898345.
Four hundred ninety-two Testyolk cycles and 579 Ham's F10 cycles were compared, with cumulative pregnancy rates at one year of 53% and 44%, respectively (P = .58). Paper-7639162.
This study demonstrates that there is no enhanced pregnancy rate or increased sperm motility when sperm are treated with Testyolk Buffer instead of Ham's F10. Paper-7639162.
Using these glass beads, we next found that exogenously added Sph-1-P inhibits F10 cell motility from outside through its specific binding site(s) on the cell. Paper-1497572.
The elimination rate constant (kel) was higher in broilers than in ducks, whereas the oral bioavailability of FX was higher in ducks than in broilers. Paper-13083943.
Two sublines of B16 melanoma cells, F10 and BL6, are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-2109347.
Thus, F24 and F39 may be intermediates in the biosynthesis of F10 and may themselves be released locally from endocrine/paracrine cells in the midgut epithelium. Paper-1250802.
RESULTS: According to the diagnoses of the primary care physicians, 7.4% of the residents had mental and behavioural disorders due to alcohol ( ICD-10: F10). Paper-2077959.
Human chorionic gonadotropin (hCG) output by early gestation cells was also 50% less in HB102 but term cells in HB102 produced twice as much hCG as those in Ham's F10. Paper-6787327.
The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X. Paper-9821087.
The factor X ( F10) genes of 14 unrelated individuals with factor X deficiency (12 familial and two sporadic cases) were sequenced yielding a total of 13 novel mutations. Paper-2164537.
Utilizing both clotting and chromogenic assays, the fibrinolysis protease plasmin was found to irreversibly inhibit the pivotal function of factor X ( FX) in coagulation. Paper-1808057.
Fresh sperm motility in 22 samples compared at 0, 6 and 24 hours in Ham's F10 (76%, 67.8%, 56.6%) versus Testyolk (76%, 67.7%, 58.8%) revealed no significant differences. Paper-7639162.
Sequence analysis of the FX-FVIII337-372 adduct suggested that the first 12 NH2-terminal residues of the FX and peptide do not participate in cross-link formation. Paper-1660813.
The F10 line, with melanin production between the B16 and F1, was intermediate in terms of inhibition of 14C-leu and 3H-TdR incorporation and dopamine binding. Paper-4000241.
These are F4 (castor oil-1% docusate sodium); F10 (castor oil-3% poloxamer 188); F14 (G33/01-10% lecithin); F17 (G33/01-1% docusate sodium), and F20 (Suppocire BP). Paper-9891183.
Luteal cells (50,000/ml) were incubated in Ham's F10 medium for 3 h at 37 degrees C either in the presence or absence of 100 ng/ml human chorionic gonadotropin (hCG). Paper-4788896.
Infection of HepG2 cells was promoted by FX but not by FVII or FIX, while transduction of CHO cells was blocked in heparan sulfate proteoglycan-deficient cells. Paper-13153086.
Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-2167753.
In the present study, we report the establishment of a monoclonal antibody (Mab) designated F10 that recognized an antigen commonly shared by human trophoblasts and leucocytes. Paper-490454.
When vascular smooth muscle cells were incubated with LDL in Ham's F10 at 37 degrees C for 48 h. release of all these substances was increased dose-dependently by oxidized LDL. Paper-701556.
Thrombin time (TT), prothrombin time (PT), and the activities of clotting factors ( FVII, FIX, FX ) in plasma contributing to the extrinsic coagulation were determined. Paper-2046169.
Tumours derived from an angioarrestin-secreting B16 ( F10) cell clone grew in vivo more slowly than tumours derived from a cell clone transfected with empty vector. Paper-11463814.
The sequence extends 1024 3' of the polyadenylation site.The gene contains 8 exons and 7 introns which were determined by comparing the mouse FX cDNA and gene sequences. Paper-8414785.
An in vivo (in C57BL/6 mice) and in vitro (in plastic plates) radiation dose-response relationship for the F10 subline of B16 melanoma was determined (200 to 1100 rads). Paper-4037626.
Morula to blastocyst stage embryos were cultured in Nutrient Mixture F10 (HAM) plus 20% fetal bovine serum gassed with 5% CO(2), 5% O(2) and 90% N(2). Paper-12091058.
Recombinant Penthalaris was expressed in insect cells and shown to inhibit factor VIIa (FVIIa)/tissue factor(TF)-induced factor X ( FX) activation with an IC50 of approximately 100 pM. Paper-10416879.
In Study II, oocytes were fertilized and cultured in either mKrb (w/ glucose or glutamine) or in a complex medium, Ham's F10 (w/ 10% fetal bovine serum [FBS]). Paper-742726.
Human coagulation factor X deficiency caused by a mutant signal peptide that blocks cleavage by signal peptidase but not targeting and translocation to the endoplasmic reticulum. Paper-7856992.
B16/ F10 pulmonary melanoma metastases were clearly visualized with (111)In-DOTA-GlyGlu-CycMSH 2 h post-injection rather than with [(18)F]FDG 1 h post-injection. Paper-13687050.
The structural gene for the human clotting factor 10 ( F10) has been mapped to chromosome 13 with a cDNA probe hybridized to DNAs from a panel of human X hamster hybrids. Paper-5249336.
2. Oocytes obtained were randomly allocated to Ham's F10 culture medium supplemented with human fetal cord serum, primate serum or commercial fetal bovine serum respectively. Paper-5466937.
A mouse hybridoma cell line producing monoclonal antibody, F10, was established from mice hyperimmunized with cells bearing adult T cell leukemia (ATL) virus ( ATLV). Paper-4700661.
F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-8488846.
The motility of spermatozoa was higher at 6-22 h in Ham's F10 culture medium supplemented with 20-30% human serum than with lower proportions of serum or with 1% human serum albumin. Paper-5320636.
Macrophage-independent oxidation of LDL, mediated by the addition of copper ions, was inhibited by cystine and cysteine in phosphate buffered saline, but not in Hams F10 medium. Paper-848480.
The deduced amino acid sequence shows 74-83% identity to group D prothrombin activators from snake venom and approximately 42% identity to mammalian FX and has identical domain structure. Paper-10947179.
Pentoxifylline (PTX), a methyl xanthine derivative, inhibits B16F10 melanoma lung homing by inhibiting F10 invasion, MMP secretion and adhesion to matrix components. Paper-12877743.
The first intron of the trocarin D gene is also nearly identical to that of the FX gene, except for two deletions (255 and 1406 bp) and three insertions (214, 1975, and 2174 bp). Paper-12433519.
Guanosine is shown to potentiate markedly the antiproliferative effect of cytosine-beta-D-arabinoside (ara-C) on B16 F10 mouse and SKMEL-28 human melanoma cell lines. Paper-7898345.
After liquefaction, 1 ml of semen was centrifuged twice in Ham's F10 medium supplemented with 10% of homologous serum, and the final suspension was used to inseminate the preovulatory eggs. Paper-5348648.
They are characterized by defective inhibition of thrombin and activated blood coagulation factor X, reduced affinity to heparin and partial immunological identity with the normal molecule. Paper-5139281.
Fertilization rate was lower (P < 0.01) in Ham's F10 but embryo development to the morulae stage and cell number per embryo were comparable (P > 0.05) for all treatments. Paper-742726.
Thus, the Pro55Ser mutation causes hemophilia primarily through to an impaired ability to activate FX whereas at least in vitro the Pro55Leu defect interferes with the activation of FIX. Paper-9932220.
Affected members of both families exhibit prolongation in prothrombin time (PT) with normal partial thromboplastin time (PTT) and low assay levels of FX, when measured by PT-based assay. Paper-12478647.
The hydrolysis of a peptide bond activates FX and the product, Xa, is yet bound to the catalytic complex in a manner such that it must leave before a new molecule of X encounters the complex. Paper-11824524.
Macrophage-independent oxidation of LDL, mediated by the addition of copper ions, was inhibited by cystine and cysteine in phosphate buffered saline, but not in Hams F10 medium. Paper-8053743.
Embryo culture was carried out in Ham's F10 supplemented with polyvinylpyrrolidone in the presence or absence of human recombinant growth factors in concentrations ranging from 1 to 100 ng/ml. Paper-1225207.
The gene for human coagulation factor X is located at 13q34, and we have found a restriction fragment length polymorphism ( RFLP) that is revealed by a cloned cDNA coding for this protein. Paper-5429663.
The effect of glucose in a modified Ham's F10 medium (MM) without hypoxanthine, phosphate and transition metals on human fertilization and sperm survival in vitro was determined. Paper-970858.
The CA-1 strain reverted in nine generations to cyromazine; however, the lowest levels of abamectin and spinosad resistance reverted to was 3.1-fold at F8 and 3.2 at the F10, respectively. Paper-10403627.
We determined the complete amino acid sequence of RVV-X, the blood coagulation factor X activating enzyme, isolated from Russell's viper venom and studied structure-function relationships. Paper-64798.
Follow-up studies were performed 3 to 4 months after delivery and the diagnoses were reconfirmed on these babies by a repeat factor assay for FX and FVII deficiency, respectively. Paper-13152984.
These findings suggest that guanosine potentiates the growth-inhibitory effect of ara-C in B16 F10 melanoma cells by increasing the intracellular concentration of its active metabolites. Paper-7898345.
In addition, flow cytometry revealed that most ( > 95%) peripheral blood lymphocytes, monocytes, as well as polymorphonuclear leukocytes (PMN) were positively stained with F10 MAb. Paper-490454.
Coagulation factor X activating enzyme from Russell's viper venom (RVV-X). A novel metalloproteinase with disintegrin ( platelet aggregation inhibitor)-like and C-type lectin-like domains. Paper-64798.
In the present study, data of a total of 27 women are presented, 11 patients with homozygous or double heterozygous deficiencies of FII, FVII and FX, together with 16 cases of hemophilia B carriers. Paper-10810412.
The HSV1 ( herpes simplex virus type 1) surface has been shown recently to initiate blood coagulation by FVIIa (activated Factor VII)-dependent proteolytic activation of FX ( Factor X). Paper-10842329.
We identified a novel deletion of exon 6 and a missense mutation (c.856G>A, Val286Met) in exon 7 of the F10 gene leading to a compound heterozygous state and causing severe factor X deficiency. Paper-13743112.
Inhibition is maximal at a CaCl2 concentration of 1.5 mM during incubation and involves the calcium dependent binding of a plasma component(s) to the TP-FVII complex, preventing the activation of FX. Paper-5449220.
Instead, thymidine appeared to augment the activity of tumor-specific cytotoxic T-cells in tumor-bearing mice, which specifically rejected a secondary challenge with the B16/ F10 tumor. Paper-1414215.
Collected fluid was centrifuged and the supernatant was frozen at -20 degrees C. For co-culture, Vero cells were commercially obtained in a frozen state and cultured using Ham's F10 medium. Paper-9204808.
This study extends previous observations of the repair of potentially lethal damage in human melanoma cells to parallel in vitro and in vivo experiments with the F10 subline of B16 melanoma. Paper-4037626.
The wide type M13 phage, 3' HVR region of alpha globin gene, F10 and various synthetic oligonucleotide probes have been shown to produce multiallelic and individual specific DNA fingerprints. Paper-499785.
The patterns of integrated virus genomes in the revertants differs markedly from that of the T637 line; one of the revertant cell lines, F10, appears to have lost all virus DNA sequences. Paper-3051362.
The deletion allele of a novel hexanucleotide insertion/deletion polymorphism in the F10 gene promoter region was shown by reporter gene assay, to reduce promoter activity by approximately 20%. Paper-2164537.
There is no specific FX replacement product yet readily available, but fresh frozen plasma and prothrombin complex concentrates can be used for treatment of bleeding symptoms and preparation for surgery. Paper-13548717.
The experimental B16 murine melanomas F1, BL-6, and F10 extract glucose at higher rates than muscle tissue, a property necessary for successful PET imaging of cutaneous melanoma. Paper-34649.
PURPOSE: Our purpose was to determine the effect of modified Ham's F10 media with and without glucose, hypoxanthine, phosphate, and transition metals (MM) on human embryo development. Paper-859757.
Treatment of the cells with the combination of FVIIa (10 nM) and FX (150 nM), reduced apoptosis by nearly 50% compared with untreated, control cells using an ELISA that detects histone-DNA fragments. Paper-12167131.
Effects of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) on murine and hamster fibroblasts (3T3 and BHK) and murine metastatic melanoma cells ( F10). Paper-5263886.
Twelve SNPs were genotyped in gilts observed for age at first estrus and ovulation rate from the F8 and F10 generations of one-quarter Meishan descendants of the USMARC resource population. Paper-12247431.
However, one isoform shows approximately 94% sequence identity with the snake FX from Tropidechis carinatus, whereas the other is much closer (90% identity) to the catalytic subunit of pseutarin C (PCCS). Paper-12108943.
RESULTS: Compared with the control subjects, platelet count, FVII, FX activities, and D-Dimer levels were significantly increased in patients with PHPT (p<0.001, p<0.05, p<0.001, and p<0.05, respectively). Paper-13074623.
Although suppression of matrix metalloprotease-9 activity may be one mechanism by which 4-HPR decreases the invasion of F10 cells, it does not appear to be the anti-invasion mechanism of NONO-AM. Paper-10823014.
Based on these findings, we compared pregnancy rates and sperm motility in intrauterine inseminations done with sperm samples washed and resuspended in Ham's F10 as compared with Testyolk buffer. Paper-7639162.
To identify host genomic sequences at the coagulation Factor X transgene integration site, DNA from a tail snip of the transgenic mouse was digested with NcoI and circularized using T4 DNA ligase. Paper-12976222.
In this report, we show that recombinant (125)I-ixolaris interacts with rat and human FX in plasma and prolongs the prothrombin time (PT) and activated partial thromboplastin time (aPTT) in vitro. Paper-12134366.
Temperature-sensitive mutants of vaccinia virus, with genetic changes that map to the open reading frame encoding the F10 protein kinase, exhibit a defect at an early stage of viral morphogenesis. Paper-10208119.
This vector was transferred to B16 ( F10) cells and recombinant angioarrestin secreted from the transfected cells was tested for anti-angiogenic activity using endothelial cell proliferation assay. Paper-11463814.
Restriction enzyme analysis placed the phi X gene A protein cleavage site in St-1 RF DNA in the HinfI restriction DNA fragment F10 and in the overlapping HaeIII restriction DNA fragment Z7. Paper-3527565.
Coagulation factor X is a serine protease containing three noncatalytic domains: an N-terminal gamma-carboxyglutamic acid (Gla)1 domain followed by two epidermal growth factor (EGF)-like domains. Paper-675872.
Compared with control subjects, no significant differences were observed in MRR30 or F30/50, whereas a higher value for F10/50 was measured (48% +/- 1% critical illness vs 40% +/- 1% control subjects, p < .01). Paper-851603.
In previous research we studied the cytotoxic effect of new Pt mercaptopyridine (MP) complexes on several tumoral cell lines ( F10, Föhn, LoVo and HeLa) as well as on the fibroblast cell line (3T3). Paper-1002621.
The objective of this study was to characterize the pharmacokinetics of a PCC (Beriplex P/N) containing coagulation factors II (FII), VII (FVII), IX (FIX) and X ( FX) and anticoagulant proteins C and S. Paper-12575763.
Activation of coagulation factor X (fX) by activated factors IX (fIXa) and VIII (fVIIIa) requires the assembly of the enzyme-cofactor-substrate fIXa-fVIIIa-fX complex on negatively charged phospholipid membranes. Paper-10829981.
In vivo, global down-regulation of vitamin K-dependent zymogens by warfarin significantly diminished liver uptake of CAR-deleted Ads; however, this phenomenon was fully rescued by acute infusion of human FX. Paper-12256137.
Surprisingly, Ham's F10 with no buffering component or with both NaHCO3 and N-Z-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) maintained sperm motility at levels similar (P > or = 0.05) to PBS. Paper-884175.
Mice given a similar treatment regimen of rHuIFN-alphaA/D had elevated natural killer (NK) cell activity as measured by in vitro cytotoxicity against YAC-I or in vivo pulmonary clearance of B16 F10 cells. Paper-4646369.
Tissue factor was detected on the cell surface of cultured human brain pericytes by immunocytochemistry and was shown to form a functional complex with factor (F) VIIa to effect both FIX and FX activation. Paper-873487.
To further study the role of the enzyme, we constructed recombinant vaccinia virus vF10V5i, which expresses inducible V5 epitope-tagged F10 and is dependent on a chemical inducer for plaque formation and replication. Paper-10208119.
Double immunofluorescence staining with F10 antibody and anti-ATLA-positive human serum caused co-capping on cell surfaces, which suggests that gp21 co-exists with other ATLV antigens expressed on cell surfaces. Paper-4700661.
We previously reported that some human antiphospholipid Abs (aPL) in patients with the antiphospholipid syndrome (APS) bind to the homologous enzymatic domains of thrombin and the activated coagulation factor X (FXa). Paper-13582997.
We suggest that F10 may be useful for the identification of a novel epitope that is commonly shared by all trophoblasts and leukocytes and such an epitope may be potentially active in maternal-fetal interactions. Paper-490454.
Increased platelet count, F VII and FX activities and D-Dimer levels in patients with PHPT represent a potential hypercoagulable state, which might augment the risk for atherosclerotic and atherothrombotic complications. Paper-13074623.
Optimal cell viability and growth were achieved using Ham's F10 medium enriched with 20% fetal bovine serum, although cells from a patient with acromegaly also grew in serum-free, defined, hormone-containing medium. Paper-4223107.
Among tumor cell products or activities which may promote clot formation, cancer procoagulant (CP), a direct activator of coagulation factor X, has been suggested to be selectively associated with the malignant phenotype. Paper-5413802.
We have compared hormone production by early gestation and term human placental trophoblasts cultured in Ham's F10 medium containing 10% fetal bovine serum with that by cells cultured in serum-free HB102 medium. Paper-6787327.
In a previous study we characterized cancer procoagulant (CP), a 68 kd cysteine proteinase which directly activates coagulation factor X in various subtypes (from M1 to M5) of acute non-lymphoblastic leukemia ( ANLL). Paper-6599776.
In the present work we characterized the interaction of Ixolaris with human FX quantitatively, and identified a precursor state of the heparin-binding exosite (proexosite, HBPE) as the Ixolaris-binding site on the zymogen. Paper-12669683.
The variability in phenotype, the uncertainty of the minimal haemostatic FX:C concentration and the association of PCC's with thrombosis make thrombin generation of interest in the management of FX deficient patients. Paper-13609259.
We show here that recombining the N-terminal subdomain from coagulation factor X with the C-terminal subdomain from trypsin creates a potent enzyme (fXYa) with novel properties, in particular a broad substrate specificity. Paper-1547278.
The haplotypes of FX alleles were determined by the following allelic variants located in the promoter: g.1323_1330delTTGTGA (A1/A2), g.1449T>C, g.1451C>A, upstream to exon 3: g.17257C>T and downstream to exon 3: g.17396A>C. Paper-13183974.
We studied B16 murine melanomas of increasing metastatic potential (F1, low; BL-6, intermediate; F10, high) as a feasibility study to determine the potential for human melanoma imaging using FDG-PET. Paper-34649.
Increased platelet count, FV and FX activities and decreased AT III levels in patients with IHH represent a potential hypercoagulable state, which might augment the risk for atherosclerotic and atherothrombotic complications. Paper-12866111.
In one patient, a reappearance of PBMC containing the huM10 gene, but not the FX gene, occurred concomitant with a rise in the HIV-1 viral load during a period of nonadherence to the antiretroviral regimen. Paper-11506015.
CONCLUSION: A detailed analysis of deletions and insertions along with the sequence indicates that the second isoform is an intermediate caught in the evolution of venom prothrombin activator from the blood coagulation FX. Paper-12108943.
The highly metastatic clone F10 of B16 melanoma did not secrete any gelatinase in neutral culture media ( pH 7.1-7.3), whereas it secreted a high level of a 103-kDa gelatinase in an initial pH range of 5.4-6. Paper-7355343.
In situ hybridization analysis localized the F10 mRNA to a variety of cell types within the central nervous system (CNS), a peripheral neurosecretory cell (L1), and midgut endocrine cells, which suggests diverse functions. Paper-9432085.
Moreover, in B16 murine melanoma cells the ability to produce ADP and to support platelet aggregation appears to be unrelated to metastatic potential insofar as identical results were obtained with the F1 and F10 variants. Paper-5817547.
At the end of the 53-day treatment period, all animals were placed under chloroform anaesthesia; the caudal epididymides were removed, minced and placed in Ham's F10 solution for the evaluation of sperm count and motility. Paper-12182656.
The complete amino acid sequence of the light chain of human blood coagulation factor X has been determined by automated Edman degradation of peptides isolated from chemical and enzymatic digests of the carboxymethylated light chain. Paper-4460677.
A toxin immunologically similar to VacA produced by other strains of Helicobacter such as H. muridarum (ATCC49282), H. mustelae ( F10) and H. felis (ATCC49179) could not be detected by Bead-ELISA as well as Western Blot. Paper-1794896.
Plasma coagulation factor VII and FX activities were significantly lower in the DD genotype (n=42) than in the II genotype (n=67, P<0.001 and P<0.001, respectively) or the ID genotype (n=63, P<0.01 and P<0.05, respectively). Paper-9937065.
The A1-C-A-T-C haplotype was found on each allele bearing the Phe31Ser mutation in the eight FX deficient patients contrasting with its low frequency (8%) in a control Algerian population (in which the Phe31Ser substitution was absent). Paper-13183974.
We have investigated a new approach to efficiently find a novel inhibitor against a serine protease (i.e. an activated coagulation factor X, FXa) by using de novo design programs and the X-ray crystal structure of the target enzyme. Paper-9949506.
Organ cultures of central and peripheral human corneal epithelium were maintained for 1 and 2 weeks in Hams F10 medium supplemented with 20% foetal bovine serum, and in medium conditioned by conjunctival epithelial cells. Paper-7067101.
These results indicate that the association of FVIIIa and FX occurs from a salt linkage(s) formed between residues of the A1 acidic C-terminus of the cofactor (within residues 349-372) and the serine protease-forming domain of the substrate. Paper-1660813.
METHODS: We identified the ACE I/D genotype and measured the plasma coagulation factor VII and X (FVII and FX) activities and serum lipids in 172 patients (110 men and 62 women, mean age 56.7+/-13.3 years) undergoing coronary angiography. Paper-9937065.
In addition, while FIX activity remained stable, there were marked reductions in mean plasma FX and FII activities and a slight decrease in FVII activity that resulted in a significant prolongation of prothrombin time within normal ranges. Paper-10543725.
Two activators of coagulation factor X, 58kDa VAFXA-I and 70kDa VAFXA-II, were purified from the venom of long-nosed viper (Vipera ammodytes ammodytes) by chromatography on gel filtration, affinity, ion-exchange and hydroxyapatite media. Paper-13036937.
However, there was no significant difference in comparison of the fertilization rate between B2 and modified Ham's F10 medium, or statistical difference between the usual 5% O2-5% CO2 and 90% N2 gas mixture for culture and 5% CO2 in air. Paper-4496252.
Considering that activated coagulation factor X (FXa) is homologous to thrombin in the catalytic domains and is also regulated primarily by AT, we hypothesized that some thrombin-reactive Ab may bind to FXa and interfere with AT inactivation of FXa. Paper-12322459.
This international study analysed the phenotype and genotype of 102 subjects from Central Europe (Germany, Poland and Slovakia) and Latin America ( Costa Rica and Venezuela) with causative mutations in the F10 gene, via sequencing. Paper-12184305.
Under conditions designed to mimic normal tissue factor-initiated hemostasis in vivo, we found that only a very small amount of FX -- equivalent to about 3% of the normal plasma level -- was required to support a 'normal' level of thrombin generation. Paper-8438062.
Coagulation factor X, when activated to factor Xa by proteolytic cleavage, itself becomes an active serine protease which participates as a component of the macromolecular prothrombinase complex along with factor Va, phospholipid, and calcium ions. Paper-7402929.
Cancer procoagulant (CP) is a cysteine protease produced by fetal and malignant tissues, activating in vitro blood coagulation factor X. It has been demonstrated that CP is able to stimulate blood platelet adhesion to fibrinogen and collagen. Paper-11211049.
By exploiting naturally occurring variants (V342A and G381D, catalytic domain; E19A, gamma-carboxyglutamic acid (GLA)-rich domain), we investigated the relationship between the pro-coagulant activity and the signal transduction capacity of FX. Paper-13693964.
Cell surface reactivity with mAbs F8 and G253, detecting a 95 kilodalton (kD) glycoprotein ( gp95); with mAbs F10 and A103, detecting a 50 kD glycoprotein ( gp50); and with mAb S7 was found to cosegregate with human chromosome 19. Paper-5646211.
In in vivo experiments, the haemostatic activity of the combination treatment of FVIIa and FX was determined by measuring the bleeding time and TEG using a monkey haemophilia B model produced by the injection of anti-human FIX polyclonal antibodies. Paper-10202932.
Electron microscopic studies of spermatozoa of retrograde ejaculates collected in Baker's buffer and washed in Ham's F10 medium supplemented with 2% human serum albumin revealed considerable ultrastructural lesions to the sperm head and the midpiece. Paper-5318364.
Of 124 oocytes incubated in tissue culture Medium 199 or Ham's F10 supplemented with 10% fetal calf serum, 52 (42%) displayed germinal vesicle breakdown and 39 (31%) progressed to metaphase of the second meiotic division within 42 hours. Paper-2397232.
The exact position and the nucleotide sequence at the 3'-OH end of the nick were determined by DNA sequence analysis of the single-stranded DNA subfragment of the nicked DNA fragment F10 obtained by gelelectrophoresis in denaturing conditions. Paper-3527565.
Parental MDA-MB-231 breast cancer cells were resistant to 4-HPR-induced apoptosis at clinically relevant doses, whereas 4-HPR-induced apoptosis in a dose-dependent manner in MDA-MB-231/ F10 bone metastatic breast cancer cells. Paper-10823014.
Preincubation of virions with heparin or pretreatment of cells with heparinase I indicated that the role of cell surface heparan sulfate during FIX- and FX-mediated adenovirus binding and infection is mechanistically serotype specific. Paper-13673034.
The extent of the acid-induced gelatinase secretion by the B16 melanoma cell lines was in the order of BL6 greater than F10 greater than F1 much greater than the parent B16 line, in good agreement with the order of their metastatic potentials. Paper-7355343.
Biodistribution experiments showed that tumor uptake values (percentage injected dose per gram of tumor [%ID/g]) of (18)F-FB-NAPamide were 1.19 +/- 0.11 %ID/g and 0.46 +/- 0.11 %ID/g, in B16/ F10 and A375M xenografted melanoma at 1 h after injection, respectively. Paper-13261549.
We have shown in previous studies that development of 8-cell hamster embryos to hatching and hatched blastocysts in vitro is stimulated by the addition to the culture medium of a group of 11 water-soluble vitamins and growth factors from Ham's F10 medium. Paper-6041935.
Using nonradioactive hybridization, the multilocal probes 33.15, F10 and (CAC)5 were shown to recognize multiple minisatellite regions in nuclear DNA of Leishmania, producing on Southern blots complex banding patterns typical of DNA fingerprints. Paper-7445452.
Three media for human semen were tested--(1) HAMS F10 supplemented with fructose, glycine and glycerol (MHAMS), (2) another chemically defined medium, but specifically designed for human semen (HSPM) and (3) medium containing egg yolk (EYCM). Paper-4183399.
Finally, the co-administration of FVIIa (80 microg/kg) and FX (800 microg/kg) in a monkey haemophilia B model resulted in a more robust and persistent haemostatic effect on the secondary bleeding time and whole-blood clotting time of TEG than that of FVIIa alone. Paper-10202932.
Three sublines of B16, F1, F10, and F1r with variable metastatic potential, and 50 B16 F1 clones all expressed the antigen with a mean antigen density above background of 8.84 +/- 2.52 [( mean cell fluorescence/ cell size] X 10(-3] ranging from 3.68 to 16.57. Paper-4838489.
The current report assesses the ability of hu-IFN-gammaR chains to transduce signals in the absence of background human gene products by expressing hu-IFN-gammaR2 in a transformed macrophage cell line ( F10/96) derived from a hu-IFN-gammaR1 transgenic mouse. Paper-822912.
Three metastatic variants, BL6 (high metastasis), F1 (nonmalignant) and F10 (intermediate malignancy) of the B16 murine melanoma, and a pulmonary metastatic line BL6-ML8 of the BL6 primary tumour have been examined for spontaneous sister chromatid exchange (SCE). Paper-6112413.
The precursor of the catalytic subunit of pseutarin C has several unique features.The activation peptide of the catalytic subunit of pseutarin C is significantly smaller (27 as compared to 52 residues in mammalian FX) and does not contain any glycosylation sites. Paper-10947179.
We investigated the action of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) in vitro on murine (3T3) and baby hamster kidney (BHK) fibroblasts and an in vivo highly metastatic subline of murine B16 melanoma ( F10). Paper-5263886.
This position appears critical for substrate specificity among the proteases of the blood coagulation cascade due to its prominent position in the macromolecular exosite and possibly via its interaction with the corresponding position in the substrate (i.e. FIX or FX). Paper-12384607.
Using Cu2+ or the free radical generator 2,2'-azobis-[2-amidinopropane] dihydrochloride (AAPH) to oxidize human LDL, we showed that the cell culture media Ham's F10 and RPMI are potent antioxidants which reduce LDL-protective effect of various thyroid compounds. Paper-10939719.
These results suggest that Arg143 and Lys147 of the autolysis loop are recognition sites for FX independent of factor VIIIa, and Arg150 is a specific recognition site for AT that can effectively interact with AT only if the serpin is in the heparin-activated conformation. Paper-9779948.
When platelets were incubated with prostacyclin, prostaglandin E1, or prostaglandin D2 at concentrations insufficient to increase the level of adenosine 3',5'-monophosphate ( cyclic AMP), coagulation factor X was activated by a platelet cysteine protease. Paper-5392876.
The antibodies are shown to bind to all the melanoma cells tested, including five different murine melanoma lines (S91, JB/RH, JB/MS, K1735, and B16), three different B16 sublines (F1, F10, and BL6), three human, one hamster, and two swine melanoma cell lines. Paper-6057112.
RESULTS: The frequency of the D allele was significantly higher in those with a history of myocardial infarction (MI) than in those with normal coronary arteries, but there was no significant association between FVII and FX activities and the stage of coronary disease. Paper-9937065.
Successful selective photothermal ablation of B16/ F10 melanoma with targeted HAuNS was confirmed by histologic and [ 18F]fluorodeoxyglucose positron emission tomography evaluation at 24 hours post near IR-region laser irradiation at a low-dose energy of 30 J/cm2. Paper-13599664.
Several types of routine media and media with human as well as foetal bovine serum (FBS) were found to support DNA synthesis in the cultured epithelium, labelled cells were not observed in epithelium maintained in Ham's F10 without serum, or with low serum concentration. Paper-6329439.
We evaluated the suitability of 3 additional disinfectants: two of these (TriGene Virucidal Disinfectant Cleaner and F10 Super Concentrate Disinfectant) are mixtures of chemicals and one ( Betadine Antiseptic Liquid) contains a single active ingredient, povidone iodine. Paper-13177974.
Recently ejaculated semen samples were washed twice to eliminate seminal plasma and a swim-up was performed to select the motile population which, in turn, was incubated up to 18 h at 37 degrees C in 3.5% human serum albumin-supplemented Ham's F10 to allow for capacitation. Paper-9906115.
When effects of DON were compared to other 8-ketotrichothecenes, the concentrations of DON, 3-acetyl deoxynivalenol (3-ADON), 15-acetyl deoxynivalenol (15-ADON), nivalenol (NIV) and fusarenon X ( FX) causing 50% apoptosis were 0.6, 0.5, 0.5, 0.5 and 7.5 microg/ml, respectively. Paper-11301548.
Variation in the antigen level of heterozygous relatives of probands was found to be significantly higher between families than within families, consistent with the view that the nature of the F10 lesion(s) segregating in a given family is a prime determinant of the laboratory phenotype. Paper-2164537.
However, very limited information is available so far on this issue; it has recently been shown that the direct activator of coagulation factor X is a vitamin K-dependent activity which is depressed by warfarin treatment, not by anticoagulation with heparin or defibrinating enzymes. Paper-4779742.
Comparison of results from the serological typing with the presence of a number of chromosome 19 DNA markers in hybrid cells and cytogenetic analysis suggests that MSK20, the gene coding for the F10/A103 antigen gp50, is located in chromosome region 19pter----19p13. Paper-5646211.
The ability of malignant tissue from 50 patients with colorectal carcinoma to activate blood coagulation factor X directly was compared with samples of adjacent, macroscopically normal colonic mucosa from the same patients, and tissue from four patients with non-malignant bowel disease. Paper-5733614.
We tested this concept in human melanoma cells cultured in either Ham's F10 medium to maintain amelanotic phenotype or DMEM to induce/stimulate melanin production, respectively; N-phenylthiourea ( PTU) and D-penicillamine were used as an inhibitor of melanogenesis. Paper-12939318.
Background: Protein Z, a vitamin K-dependent glycoprotein, serves as a cofactor for the inhibition of activated coagulation factor X. During recent years, a role for low levels of protein Z in prothrombotic disorders such as ischemic stroke and acute coronary syndromes (ACS) has been reported. Paper-12214923.
In the present study, we searched for an effective combination of plasma-derived FVIIa with other blood coagulation factors, and demonstrated that a therapeutic approach combining plasma-derived FVIIa and Factor X ( FX) was more useful for treating haemophiliacs with inhibitors than FVIIa alone. Paper-10202932.
CONCLUSIONS: Our data demonstrate that platelet activation, factor XII-driven thrombus formation, and plasmatic coagulation pathways downstream of FX are not a prerequisite for ensuing tissue damage in models of photothrombotic vessel injury indicating that other pathomechanisms are involved. Paper-12769275.
Sperm from nine giant pandas were assessed before and after rapid (- 40 and - 100 degrees C/min) cryopreservation by incubation in HEPES-buffered Ham's F10 medium with and without the capacitation accelerators, 3-isobutyl-1-methylxanthine (IBMX) and dibutyryl cyclic AMP ( dbcAMP). Paper-10354694.
This study indicated that Bothrops atrox venom possesses a thrombin-like action caused by one or several serine proteases sensitive to DFP treatment, but that its clotting action is in fact mainly due to components insensitive to DFP which activate prothrombin and factor X ( Stuart factor). Paper-4503197.
International Classification of Diseases, Tenth Revision ( ICD-10) codes were used to identify the presence of HCV (B17.1 and B18.2) and HAU (as indicated by alcohol-induced medical conditions, F10 and K70) either as the underlying cause or as one of the contributing causes of death. Paper-12437167.
Hence, it was concluded that F7, F8 and F10 caused haemagglutination and were responsible for the attachment to urinary epithelial cells, while the fimbriae antigenically termed pseudotype 1 did not induce haemagglutination and played little or no role for the attachment to this kind of epithelial cells. Paper-4201808.
Coumarin derivatives and anticonvulsants administered during pregnancy enter the fetal circulation, interfering with the action of vitamin K. Vitamin K plays a crucial part in the gamma-carboxylation of glutamic acid residues of the vitamin K-dependent coagulation factors prothrombin, FVII, FIX, and FX. Paper-682342.
These are a procoagulant activity, which is present on the cell membrane of LPS-treated rabbit liver M phi and acts by directly activating coagulation factor X, and a factor released into the supernatant by LPS-treated peritoneal exudate M phi, which suppresses steroidogenesis in explanted adrenocortical cells. Paper-4631771.
The previous suggestion that there is a hairpin loop involving residues Gly(12) to Val(15) in the A alpha chain of human fibrinogen is supported by the slow backbone NH exchange rates of Gly(14) and Val(15), by an unusually small NH chemical shift of Val(15), and by strong sequential NOE's involving this region in F10. Paper-6082163.
Comparison of the in vitro data with our earlier observations that cat's claw and Echinacea (but not saw palmetto) were each effective in reducing B16/ F10 lung tumor colony formation in C57BL/6J mice suggests macrophage activation is the primary means by which these herbs modulate the immune system. Paper-13218151.
In every subject the following measurements were performed: albuminemia before (t0) warfarin administration; plasma warfarin and vitamin K-dependent coagulation factors (FII, FVII, FIX, FX) levels, before and at time intervals from 0 to 48 h after drug administration; warfarin urinary excretion from 0 to 24 h. Paper-5228921.
When 10(5) cells per 60 mm dish were exposed to MNNG for 4 h in Ham's medium F10 containing 0.02 M HEPES buffer at pH 6.8, MNNG concentrations of less than 1 X 10(-6) M resulted in cell survivals near 100%, while the average survival was less than one percent at concentrations greater than 5 X 10(-6) M. Paper-3064995.
The fluorescence polarization of diphenylhexatriene ( DPH) and trimethylammonium diphenylhexatriene (TMA-DPH) was measured when these markers were imbedded in cells of the human melanoma cell lines IGR37, IGR39, IGR3 and IGR4, as well as in cells of the mouse melanoma cell lines B16F1 and B16 F10. Paper-4705607.
Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. Paper-8008467.
The resulting variant exhibited increased intrinsic activity, concurrent with maturation of the active site, a less accessible N-terminus, and, interestingly, an altered macromolecular substrate specificity reflected in an increased ability to cleave factor IX (FIX) and a decreased rate of FX activation compared to that of wild-type FVIIa. Paper-12384607.
The effect of a human recombinant hybrid alpha interferon (referred to as rHuIFN-alphaA/D) on pulmonary metastases induced by intravenous injection of B16 F10 melanoma cells in C57BL/6 mice was examined; rHuIFN-alphaA/D has been previously shown to have anti-viral, anti-proliferative and immunomodulatory activities in murine cells. Paper-4646369.
Peripheral blood mononuclear cells (PBMC) containing the huM10 gene or FX gene were detected by quantitative PCR at frequencies of approximately 1/10,000 in both subjects for the first 1-3 months following re-infusion of the gene-transduced bone marrow, but then were at or below the limits of detection (<1/1,000,000) at most times over 2 years. Paper-11506015.
B16 melanoma (B16M) cells with high glutathione (GSH) content show rapid proliferation in vitro and high metastatic activity in the liver in vivo. gamma-Glutamyl transpeptidase (GGT)-mediated extracellular GSH cleavage and intracellular GSH synthesis were studied in vitro in B16M cells with high ( F10) and low (F1) metastatic potential. Paper-9354225.
A total of 460 fertilized oocytes from 82 cycles of patients was transferred into one of four systems: (1) into droplets of Ham's F10 medium + 12% normal human serum (NHS); (2) co-cultured on a human granulosa monolayer; (3) co-cultured with bovine oviductal epithelial cells (BOEC); or (4) co-cultured with bovine uterine epithelial cells (BUEC). Paper-698210.
These synonyms are used for gene F10 (coagulation factor X): Stuart-Prower factor, Stuart factor, FXA, FX, Coagulation factor X precursor.
These accession numbers are used for gene F10: Q5JVE8 (UNIPROT__AC), O15095 (UNIPROT__AC).
F10 is a homologue of F10 (coagulation factor X) from Canis familiaris.
F10 is a homologue of F10 (coagulation factor X) from Bos taurus.
F10 is a homologue of F10 (coagulation factor X) from Bos taurus.
F10 is a homologue of F10 (coagulation factor X) from Gallus gallus.
F10 is a homologue of F10 (coagulation factor X) from Mus musculus.
F10 is a homologue of F10 (coagulation factor X) from Pan troglodytes.
F10 is a homologue of F10 (coagulation factor X) from Rattus norvegicus.
F10 is a homologue of f10 (coagulation factor X) from Danio rerio.
F10 is a homologue of CG11836 (CG11836) from Drosophila melanogaster.
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iHOP - Information Hyperlinked over Proteins .
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