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Activation of coagulation factor X in human semen. Paper-1514721.
Severe sepsis results in an elevation of F10/ 50 only. Paper-851603.
Of the three peptides, only F10 was found in the hemolymph. Paper-1250802.
Three murine melanomas (F1, F10, B16) were studied. Paper-4000241.
This suggests a broad role for FX in adenovirus infectivity. Paper-13153086.
Congenital factor X (Stuart-Prower factor) deficiency: a family study. Paper-6016878.
Oral contraceptives caused increased fibrinogen, FVII, FX, and fibrinolysis. Paper-7385427.
Kinetics of coagulation factor X activation by platelet-bound factor IXa. Paper-6538645.
SCE was encountered in 29 and 13% of second division metaphases of BL6 and F10. Paper-6112413.
FX genes of the affected family members were analyzed by sequence analysis. Paper-12478647.
The cumulus cell monolayer was prepared in follicular fluid (FC) and Ham's F10 (HC). Paper-10987159.
Coagulation factor X also binds to Mac-1 on monocytes at a similar site to C3bi. Paper-126851.
Activated coagulation factor X: a novel mitogenic stimulus for human mesangial cells. Paper-8795174.
The structural gene for human coagulation factor X is located on chromosome 13q34. Paper-4927783.
In addition, ixolaris interacts with FX possibly through the heparin-binding proexosite. Paper-10732673.
Improved human zygote development in a modified Ham's F10 medium in vitro. Paper-859757.
Prostacyclin stimulation of the activation of blood coagulation factor X by platelets. Paper-5392876.
Impaired human embryo development in Earle's culture medium compared to Ham's F10. Paper-7425232.
LGMM did not appear to improve the pregnancy outcome compared to normal Ham's F10. Paper-859757.
The proband is the first reported case of homozygote for the FX Gly366Ser mutation. Paper-11239410.
The number of SCEs per chromosome was twice as high in F10, BL6 and BL6-ML8 as in the F1 cells. Paper-6112413.
Cloning and expression in COS-1 cells of a full-length cDNA encoding human coagulation factor X. Paper-31206.
An aliquot was also incubated for 60 min and Ham's F10 medium containing 50 USP/ml heparin. Paper-1701958.
Intracranial hemorrhage (ICH) is a severe complication of Factor X ( FX) deficiency. Paper-11187208.
The treatment groups were as follows: normal Hams F10, MM, and MM with 0.5 mM glucose (LGMM). Paper-859757.
Evidence for an essential catalytic role of the F10 protein kinase in vaccinia virus morphogenesis. Paper-10208119.
Differences between human and rabbit coagulation factor X-implications for in vivo models of thrombosis. Paper-9603336.
Acquired, transient factor X ( Stuart factor) deficiency in patient with mycoplasma pneumonial infection. Paper-3411484.
The effect of calcium (II) on the binding of anticoagulation factor I with activated coagulation factor X. Paper-8786126.
In this system, BL6 cells could transfer dye into endothelial cells but F10 cells could not. Paper-8488846.
The coagulation factor X activator from Russell's viper venom (RVV-X) is a heterotrimeric glycoprotein. Paper-12908875.
These FX variants should be useful for site-specific induction of blood coagulation in the tumor vasculature. Paper-11542479.
Classical hemophilia results from a defect of the intrinsic tenase complex, the main factor X ( FX) activator. Paper-11153360.
The patients with FII, FVII or FX defects had a total of 14 pregnancies and often needed transfusion therapy. Paper-10810412.
When supplemented with protein, Ham's F10 was the poorest of the media in supporting embryo development. Paper-6688531.
Washed human healthy sperm were suspended in Ham's F10 and exposed to varying concentrations of curcumin. Paper-10102199.
Finally, we were able to define a linear epitope recognized by the Ul/ F10 antibody on the nucleocapsid protein. Paper-122123.
A flow cytometric method for determining the binding of coagulation factor X to monocytes in whole human blood. Paper-10658816.
Russel viper venom directly activates FX, in presence of PF3, is an indicator of common pathway of coagulation. Paper-13391054.
Pharmacokinetics and pharmacodynamics of the dual FII/ FX inhibitor BIBT 986 in endotoxin-induced coagulation. Paper-13242229.
Inactivation of human blood coagulation factor X by chemical modification of gamma-carboxyglutamic acid residues. Paper-4661326.
Renewing the medium (Ham's F10 with 30% serum) at 7 h resulted in better sperm motility and velocity at 22 h. Paper-5320636.
Also after 24 h, the percentage of spermatozoa which were highly motile was greater in HGMM than in Ham's F10. Paper-970858.
F10 MAb was obtained using cell membrane components from a trophoblast cell line HT as immunogen. Paper-490454.
In this study we investigated anti-tumour properties of angioarrestin in B16 ( F10) melanoma tumour model. Paper-11463814.
F9 and F10 also cross-reacted with the antisera against ascariasis and F15, with antisera against angiostrongylosis. Paper-1558803.
Herpes simplex virus type 1-encoded glycoprotein C contributes to direct coagulation factor X-virus binding. Paper-10842329.
However, motility rapidly decreased (P < 0.05) with the addition of NaHCO3 to PBS or Ham's F10 nutrient mixture. Paper-884175.
Gel-filtration chromatography demonstrated 1:1 complex formation between fluorescein-labeled Ixolaris and FX. Paper-12669683.
These studies provide evidence for an essential catalytic role of the F10 kinase in vaccinia virus morphogenesis. Paper-10208119.
Blood coagulation Factor X is a serine protease required for both the intrinsic and extrinsic pathways of coagulation. Paper-65900.
Stuart Prower factor (Factor X) deficiency is a rare hereditary autosomal recessive coagulation disorder. Paper-11107412.
Furthermore, cellular oxidation of LDL could be partially mimicked by the addition of cysteine to Hams F10 medium. Paper-848480.
In all three cases, the hybridization pattern F10 - G9 - PT1526 indicates a centromere to telomere orientation. Paper-8852302.
We assessed the vaccine for its prophylactic and therapeutic effect against the murine B16 F10 melanoma. Paper-9386795.
Blood coagulation Factor X and its activated form Factor Xa play an essential role in the midphase of the clotting cascade. Paper-477709.
Furthermore, cellular oxidation of LDL could be partially mimicked by the addition of cysteine to Hams F10 medium. Paper-8053743.
The leukocyte beta2-integrins have several functions, including serving as receptors for coagulation factor X and fibrinogen. Paper-1662108.
Identification of the oligosaccharide structures of human coagulation factor X activation peptide at each glycosylation site. Paper-300604.
Complex media (RPMI 1640 and Ham's F10) generally caused a greater progesterone release than simple salt solution (EBSS). Paper-7074611.
Liver infection by the FX-Ad5 complex is mediated through a heparin-binding exosite in the FX serine protease domain. Paper-12736621.
Activation of coagulation factor X via the intrinsic pathway requires the assembly of factors IXa and VIII on lipid membranes. Paper-6952195.
There was a significant association between serum triglyceride levels with FVII and FX, but not with the ACE I/D genotype. Paper-9937065.
In this study we explored the use of human maternal serum (HMS) from early gestation as the medium supplement to Ham's F10. Paper-7867207.
The highly metastatic line, B16 F10, forms less prostaglandin D2 compared to the moderately metastatic parent line, B16 F1. Paper-3334758.
Antibody phage display was employed to isolate two scFv antibody fragments, D8 and F10, with specificity for the VEGF165 isoform. Paper-9790137.
With husband insemination, 264 Testyolk and 253 Ham's F10 cycles had pregnancy rates of 37% and 35%, respectively (P = .23). Paper-7639162.
Directed glycosylation of human coagulation factor X at residue 333. Insight into factor Va-dependent prothrombin catalysis. Paper-8640272.
No predominant structure was found in the chain segment from Ala(1) to Gly(6) for F10 in both H2O and dimethyl sulfoxide. Paper-6082163.
Unlike 4-HPR, NONO-AM induced apoptosis in a dose-dependent manner in both parental MDA-MB-231 cells and F10 cells. Paper-10823014.
Tissue factor (TF)-bound factor (F)VIIa plays a critical role in activating FX, an event that rapidly results in blood coagulation. Paper-10084627.
F10 antibody reacted with an ATLV structural polypeptide ( gp21 ) with a m.w. of 21,000 that was glycosylated on cell surfaces. Paper-4700661.
In situ hybridization was used to assign F10 to region 13q32----qter of chromosomes from normal human lymphocytes. Paper-5249336.
A number of studies suggest that blood-clotting factor X ( FX) uses secondary site(s) to interact (as a substrate) with its activators. Paper-10328116.
The construct was transfected into dexamethasone receptor negative F1 and receptor positive F10 cells of the B16 murine melanoma. Paper-138567.
Treatment of mice with rHuIFN-alphaA/D following B16 F10 injection resulted in no significant inhibition of pulmonary metastases. Paper-4646369.
During blood coagulation, factor IXa (FIXa) activates factor X ( FX) requiring Ca2+, phospholipid, and factor VIIIa (FVIIIa). Paper-10980781.
The association of the ACE D allele with lower activities of FVII and FX was also seen in patients with coronary artery disease (CAD). Paper-9937065.
Human FX substantially enhanced hepatocyte transduction by CAR-permissive and mutated viruses in an ex vivo liver perfusion model. Paper-12256137.
The highly metastatic F10 subline of the B16 melanoma was maintained in tissue culture to assure high NK sensitivity of the cells. Paper-4627736.
We investigated the antitumor effects of human recombinant interleukin-6 (hrIL-6) on the highly metastatic B16 melanoma clone F10. Paper-7866184.
Fifty microg/ml LDL protein was incubated with macrophages in Ham's F10 medium, supplemented with additional Fe2+, for up to 48 h. Paper-1871722.
Two cases of Hansen disease demonstrating a lupus-like inhibitor directed against the activation of coagulation factor X are described. Paper-3596091.
The bone metastatic F10 cells were more sensitive to the anti-invasive effects of 4-HPR and NONO-AM than were MDA-MB-231 cells. Paper-10823014.
Activation of blood coagulation factor X by arginine-specific cysteine proteinases (gingipain-Rs) from Porphyromonas gingivalis. Paper-1065918.
The gene coding for coagulation factor X was studied in a family segregating chromosomal abnormalities involving chromosomes 13 and 6. Paper-4927783.
Oocytes classified as mature were cultured for 0, 2, 4, 6, or 8 h in Ham's F10 medium and then stained for nuclear maturation. Paper-8145596.
The embryos of the C57BL X CBA strain showed the highest hatching rate in BWW but the expanded blastocyst rate was highest in F10. Paper-5952717.
All patients were homozygous for a novel FX mutation (Gly381Asp) in the structurally conserved region of the serine protease active site. Paper-9828314.
P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Paper-3683145.
Measurement of the kinetics of inhibition of activated coagulation factor X in human plasma: the effect of plasma and inhibitor concentration. Paper-5300016.
With donor sperm, 229 cycles with Testyolk and 314 cycles with Ham's F10 had cumulative pregnancy rates of 68% and 48%, respectively (P = .52). Paper-7639162.
Fertilization rates were significantly lower in oocytes cultured in MM (23.8%) compared to LGMM (75.5%), HGMM (73.6%) or Ham's F10 (71.1%). Paper-970858.
In the sepsis patient group, a significantly higher F10/50 was measured (52% +/- 3% severe sepsis vs 40% +/- 1% control subjects, p < .01). Paper-851603.
Upon complex formation with its cofactor FVIIIa and Ca2+-mediated binding to phospholipid membranes, FIXa becomes a very potent activator of FX. Paper-1125614.
The F10 gene encodes a 52-kDa serine/ threonine protein kinase (protein kinase 2), an essential protein involved in virus morphogenesis. Paper-876804.
Blastocyst formation was lower (P < 0.05) on oviductal cells than in mKrb-Ham's F10 treatment and was < 20% in all treatments. Paper-742726.
The potential link between ACE I/D polymorphism and the plasma activities of FVII and FX is probably independent of triglyceride metabolism. Paper-9937065.
Family studies were performed in order to distinguish the contributions of individual mutant F10 alleles to the clinical and laboratory phenotypes. Paper-2164537.
RESULTS: The patient we studied was homozygous for a substitution of arginine for serine at codon -30 in the signal sequence of F10. Paper-11525610.
Anticoagulant and antithrombotic properties of a gamma-carboxyglutamic acid-rich peptide derived from the light chain of blood coagulation factor X. Paper-5333931.
Penetrance and expressivity of the gene for double podding was studied in an F2 population and F10 recombinant inbred lines (RILs) of this cross. Paper-8367605.
Antibodies against the Activated Coagulation Factor X (FXa) in the Antiphospholipid Syndrome That Interfere with the FXa Inactivation by Antithrombin. Paper-12322459.
Searching the literature, we discovered only 9 additional patients with prothrombin deficiency or FX deficiency, having a total of 16 pregnancies. Paper-10810412.
F10 remained associated with the particulate core fraction of mature virions after treatment with a nonionic detergent and reducing agent. Paper-10208120.
LDL, preincubated with 5 microM copper or with macrophages in Ham's F10 medium, accumulated in macrophages much more than did native LDL. Paper-7951999.
This integral membrane glycoprotein forms a ternary complex with factor VIIa (FVIIa) and zymogen factor ( FX), which is then activated to factor Xa (FXa). Paper-12620406.
It was found that thymidine did not increase the cytotoxicity of BCNU for B16/ F10 melanoma or L1210 leukemia cells in vitro. Paper-1414215.
The identity of the insert in a selected phage lambda clone was confirmed to be FX by nucleotide (nt) sequence analysis and restriction mapping. Paper-31206.
We generated an amphotropic retroviral vector with the human FX cDNA and delivered it to rat hepatocytes in vivo during liver regeneration. Paper-905791.
In addition, sperm motility in fresh sperm was observed in samples incubated in Testyolk or Ham's F10, with motility counts performed at 0, 6 and 24 hours. Paper-7639162.
The bleeding patterns of the enrolled patients showed differences that are associated with the types of F10 mutation, and the corresponding genotypes. Paper-12184305.
The NTC-121 cells were injected intracranially in C57BL/6 mice (N = 10/group) along with non-irradiated, non-transfected B16/ F10 (wild type) melanoma cells. Paper-9963979.
We began our research by elaborating a method for the construction of subtracted cDNA libraries, and made it applicable to BL6 and F10 cells. Paper-9603441.
Inhibition of the tissue factor-factor VII mediated activation of coagulation factor X by a monoclonal IgM antibody expressing lupus anticoagulant activity. Paper-6539887.
Spermatozoa from 15 fertile men were washed with Ham's F10 and incubated with two commercially available sperm nutrient media for 2, 4, 6, and 24 h. Paper-5965917.
The effect of Platinum (II) complexes with mercaptopyridines on cell lines ( fibroblasts 3T3 and the tumour ones F10, Föhn, Lovo) were studied. Paper-7814376.
Semen was collected in 15 mL medium (Ham's F10 [Gibco, Grand Island, NY] + 10% whole blood serum) and prepared with the wash and swim-up method. Paper-6619104.
After chorionic villus biopsy of human placenta, cell cultures were propagated with Ham's F10 medium or Eagle's minimum essential medium (MEM). Paper-262757.
The activation of human blood coagulation factor X on the surface of endothelial cells: a comparison with various vascular cells, platelets and monocytes. Paper-140067.
The lowest concentrations capable of 100% kill after exposure for 1 min were 0.1 ml l(-1) for TriGene, 0.33 ml l(-1) for F10 and 100 ml l(-1) for Betadine. Paper-13177974.
Complete amino acid sequence of the light chain of human blood coagulation factor X: evidence for identification of residue 63 as beta-hydroxyaspartic acid. Paper-4460677.
The transferred gene was switched on in two transfectant clones of F10, by exposure to 10(-6) M dexamethasone, but not in clones of the receptor negative F1 line. Paper-138567.
Procoagulant activity was demonstrated to activate prothrombin in rat uterus, to activate human coagulation factor X, and to cause clot formation by human plasma. Paper-6595165.
FX derivatives were constructed in which point mutations were made or parts of h163-170 were substituted with the corresponding region of either FVIIa or FIXa. Paper-12527883.
Several metabolic consequences of the synergistic interaction between ara-C and guanosine on cell growth were determined in B16 F10 mouse melanoma cells. Paper-7898345.
Four hundred ninety-two Testyolk cycles and 579 Ham's F10 cycles were compared, with cumulative pregnancy rates at one year of 53% and 44%, respectively (P = .58). Paper-7639162.
This study demonstrates that there is no enhanced pregnancy rate or increased sperm motility when sperm are treated with Testyolk Buffer instead of Ham's F10. Paper-7639162.
Using these glass beads, we next found that exogenously added Sph-1-P inhibits F10 cell motility from outside through its specific binding site(s) on the cell. Paper-1497572.
The elimination rate constant (kel) was higher in broilers than in ducks, whereas the oral bioavailability of FX was higher in ducks than in broilers. Paper-13083943.
Two sublines of B16 melanoma cells, F10 and BL6, are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-2109347.
Thus, F24 and F39 may be intermediates in the biosynthesis of F10 and may themselves be released locally from endocrine/paracrine cells in the midgut epithelium. Paper-1250802.
RESULTS: According to the diagnoses of the primary care physicians, 7.4% of the residents had mental and behavioural disorders due to alcohol ( ICD-10: F10). Paper-2077959.
Human chorionic gonadotropin (hCG) output by early gestation cells was also 50% less in HB102 but term cells in HB102 produced twice as much hCG as those in Ham's F10. Paper-6787327.
The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X. Paper-9821087.
The factor X ( F10) genes of 14 unrelated individuals with factor X deficiency (12 familial and two sporadic cases) were sequenced yielding a total of 13 novel mutations. Paper-2164537.
Utilizing both clotting and chromogenic assays, the fibrinolysis protease plasmin was found to irreversibly inhibit the pivotal function of factor X ( FX) in coagulation. Paper-1808057.
Fresh sperm motility in 22 samples compared at 0, 6 and 24 hours in Ham's F10 (76%, 67.8%, 56.6%) versus Testyolk (76%, 67.7%, 58.8%) revealed no significant differences. Paper-7639162.
Sequence analysis of the FX-FVIII337-372 adduct suggested that the first 12 NH2-terminal residues of the FX and peptide do not participate in cross-link formation. Paper-1660813.
The F10 line, with melanin production between the B16 and F1, was intermediate in terms of inhibition of 14C-leu and 3H-TdR incorporation and dopamine binding. Paper-4000241.
These are F4 (castor oil-1% docusate sodium); F10 (castor oil-3% poloxamer 188); F14 (G33/01-10% lecithin); F17 (G33/01-1% docusate sodium), and F20 (Suppocire BP). Paper-9891183.
Luteal cells (50,000/ml) were incubated in Ham's F10 medium for 3 h at 37 degrees C either in the presence or absence of 100 ng/ml human chorionic gonadotropin (hCG). Paper-4788896.
Infection of HepG2 cells was promoted by FX but not by FVII or FIX, while transduction of CHO cells was blocked in heparan sulfate proteoglycan-deficient cells. Paper-13153086.
Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-2167753.
In the present study, we report the establishment of a monoclonal antibody (Mab) designated F10 that recognized an antigen commonly shared by human trophoblasts and leucocytes. Paper-490454.
When vascular smooth muscle cells were incubated with LDL in Ham's F10 at 37 degrees C for 48 h. release of all these substances was increased dose-dependently by oxidized LDL. Paper-701556.
Thrombin time (TT), prothrombin time (PT), and the activities of clotting factors ( FVII, FIX, FX ) in plasma contributing to the extrinsic coagulation were determined. Paper-2046169.
Tumours derived from an angioarrestin-secreting B16 ( F10) cell clone grew in vivo more slowly than tumours derived from a cell clone transfected with empty vector. Paper-11463814.
The sequence extends 1024 3' of the polyadenylation site.The gene contains 8 exons and 7 introns which were determined by comparing the mouse FX cDNA and gene sequences. Paper-8414785.
An in vivo (in C57BL/6 mice) and in vitro (in plastic plates) radiation dose-response relationship for the F10 subline of B16 melanoma was determined (200 to 1100 rads). Paper-4037626.
Morula to blastocyst stage embryos were cultured in Nutrient Mixture F10 (HAM) plus 20% fetal bovine serum gassed with 5% CO(2), 5% O(2) and 90% N(2). Paper-12091058.
Recombinant Penthalaris was expressed in insect cells and shown to inhibit factor VIIa (FVIIa)/tissue factor(TF)-induced factor X ( FX) activation with an IC50 of approximately 100 pM. Paper-10416879.
In Study II, oocytes were fertilized and cultured in either mKrb (w/ glucose or glutamine) or in a complex medium, Ham's F10 (w/ 10% fetal bovine serum [FBS]). Paper-742726.
Human coagulation factor X deficiency caused by a mutant signal peptide that blocks cleavage by signal peptidase but not targeting and translocation to the endoplasmic reticulum. Paper-7856992.
The structural gene for the human clotting factor 10 ( F10) has been mapped to chromosome 13 with a cDNA probe hybridized to DNAs from a panel of human X hamster hybrids. Paper-5249336.
2. Oocytes obtained were randomly allocated to Ham's F10 culture medium supplemented with human fetal cord serum, primate serum or commercial fetal bovine serum respectively. Paper-5466937.
A mouse hybridoma cell line producing monoclonal antibody, F10, was established from mice hyperimmunized with cells bearing adult T cell leukemia (ATL) virus ( ATLV). Paper-4700661.
F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Paper-8488846.
The motility of spermatozoa was higher at 6-22 h in Ham's F10 culture medium supplemented with 20-30% human serum than with lower proportions of serum or with 1% human serum albumin. Paper-5320636.
Macrophage-independent oxidation of LDL, mediated by the addition of copper ions, was inhibited by cystine and cysteine in phosphate buffered saline, but not in Hams F10 medium. Paper-848480.
The deduced amino acid sequence shows 74-83% identity to group D prothrombin activators from snake venom and approximately 42% identity to mammalian FX and has identical domain structure. Paper-10947179.
Pentoxifylline (PTX), a methyl xanthine derivative, inhibits B16F10 melanoma lung homing by inhibiting F10 invasion, MMP secretion and adhesion to matrix components. Paper-12877743.
The first intron of the trocarin D gene is also nearly identical to that of the FX gene, except for two deletions (255 and 1406 bp) and three insertions (214, 1975, and 2174 bp). Paper-12433519.
Guanosine is shown to potentiate markedly the antiproliferative effect of cytosine-beta-D-arabinoside (ara-C) on B16 F10 mouse and SKMEL-28 human melanoma cell lines. Paper-7898345.
After liquefaction, 1 ml of semen was centrifuged twice in Ham's F10 medium supplemented with 10% of homologous serum, and the final suspension was used to inseminate the preovulatory eggs. Paper-5348648.
They are characterized by defective inhibition of thrombin and activated blood coagulation factor X, reduced affinity to heparin and partial immunological identity with the normal molecule. Paper-5139281.
Fertilization rate was lower (P < 0.01) in Ham's F10 but embryo development to the morulae stage and cell number per embryo were comparable (P > 0.05) for all treatments. Paper-742726.
Thus, the Pro55Ser mutation causes hemophilia primarily through to an impaired ability to activate FX whereas at least in vitro the Pro55Leu defect interferes with the activation of FIX. Paper-9932220.
Affected members of both families exhibit prolongation in prothrombin time (PT) with normal partial thromboplastin time (PTT) and low assay levels of FX, when measured by PT-based assay. Paper-12478647.
The hydrolysis of a peptide bond activates FX and the product, Xa, is yet bound to the catalytic complex in a manner such that it must leave before a new molecule of X encounters the complex. Paper-11824524.
Macrophage-independent oxidation of LDL, mediated by the addition of copper ions, was inhibited by cystine and cysteine in phosphate buffered saline, but not in Hams F10 medium. Paper-8053743.
Embryo culture was carried out in Ham's F10 supplemented with polyvinylpyrrolidone in the presence or absence of human recombinant growth factors in concentrations ranging from 1 to 100 ng/ml. Paper-1225207.
The gene for human coagulation factor X is located at 13q34, and we have found a restriction fragment length polymorphism ( RFLP) that is revealed by a cloned cDNA coding for this protein. Paper-5429663.
The effect of glucose in a modified Ham's F10 medium (MM) without hypoxanthine, phosphate and transition metals on human fertilization and sperm survival in vitro was determined. Paper-970858.
The CA-1 strain reverted in nine generations to cyromazine; however, the lowest levels of abamectin and spinosad resistance reverted to was 3.1-fold at F8 and 3.2 at the F10, respectively. Paper-10403627.
We determined the complete amino acid sequence of RVV-X, the blood coagulation factor X activating enzyme, isolated from Russell's viper venom and studied structure-function relationships. Paper-64798.
Follow-up studies were performed 3 to 4 months after delivery and the diagnoses were reconfirmed on these babies by a repeat factor assay for FX and FVII deficiency, respectively. Paper-13152984.
These findings suggest that guanosine potentiates the growth-inhibitory effect of ara-C in B16 F10 melanoma cells by increasing the intracellular concentration of its active metabolites. Paper-7898345.
In addition, flow cytometry revealed that most ( > 95%) peripheral blood lymphocytes, monocytes, as well as polymorphonuclear leukocytes (PMN) were positively stained with F10 MAb. Paper-490454.
Coagulation factor X activating enzyme from Russell's viper venom (RVV-X). A novel metalloproteinase with disintegrin ( platelet aggregation inhibitor)-like and C-type lectin-like domains. Paper-64798.
In the present study, data of a total of 27 women are presented, 11 patients with homozygous or double heterozygous deficiencies of FII, FVII and FX, together with 16 cases of hemophilia B carriers. Paper-10810412.
The HSV1 ( herpes simplex virus type 1) surface has been shown recently to initiate blood coagulation by FVIIa (activated Factor VII)-dependent proteolytic activation of FX ( Factor X). Paper-10842329.
Inhibition is maximal at a CaCl2 concentration of 1.5 mM during incubation and involves the calcium dependent binding of a plasma component(s) to the TP-FVII complex, preventing the activation of FX. Paper-5449220.
Instead, thymidine appeared to augment the activity of tumor-specific cytotoxic T-cells in tumor-bearing mice, which specifically rejected a secondary challenge with the B16/ F10 tumor. Paper-1414215.
Collected fluid was centrifuged and the supernatant was frozen at -20 degrees C. For co-culture, Vero cells were commercially obtained in a frozen state and cultured using Ham's F10 medium. Paper-9204808.
This study extends previous observations of the repair of potentially lethal damage in human melanoma cells to parallel in vitro and in vivo experiments with the F10 subline of B16 melanoma. Paper-4037626.
The wide type M13 phage, 3' HVR region of alpha globin gene, F10 and various synthetic oligonucleotide probes have been shown to produce multiallelic and individual specific DNA fingerprints. Paper-499785.
The patterns of integrated virus genomes in the revertants differs markedly from that of the T637 line; one of the revertant cell lines, F10, appears to have lost all virus DNA sequences. Paper-3051362.
The deletion allele of a novel hexanucleotide insertion/deletion polymorphism in the F10 gene promoter region was shown by reporter gene assay, to reduce promoter activity by approximately 20%. Paper-2164537.
The experimental B16 murine melanomas F1, BL-6, and F10 extract glucose at higher rates than muscle tissue, a property necessary for successful PET imaging of cutaneous melanoma. Paper-34649.
PURPOSE: Our purpose was to determine the effect of modified Ham's F10 media with and without glucose, hypoxanthine, phosphate, and transition metals (MM) on human embryo development. Paper-859757.
Treatment of the cells with the combination of FVIIa (10 nM) and FX (150 nM), reduced apoptosis by nearly 50% compared with untreated, control cells using an ELISA that detects histone-DNA fragments. Paper-12167131.
Effects of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) on murine and hamster fibroblasts (3T3 and BHK) and murine metastatic melanoma cells ( F10). Paper-5263886.
Twelve SNPs were genotyped in gilts observed for age at first estrus and ovulation rate from the F8 and F10 generations of one-quarter Meishan descendants of the USMARC resource population. Paper-12247431.
However, one isoform shows approximately 94% sequence identity with the snake FX from Tropidechis carinatus, whereas the other is much closer (90% identity) to the catalytic subunit of pseutarin C (PCCS). Paper-12108943.
RESULTS: Compared with the control subjects, platelet count, FVII, FX activities, and D-Dimer levels were significantly increased in patients with PHPT (p<0.001, p<0.05, p<0.001, and p<0.05, respectively). Paper-13074623.
Although suppression of matrix metalloprotease-9 activity may be one mechanism by which 4-HPR decreases the invasion of F10 cells, it does not appear to be the anti-invasion mechanism of NONO-AM. Paper-10823014.
Based on these findings, we compared pregnancy rates and sperm motility in intrauterine inseminations done with sperm samples washed and resuspended in Ham's F10 as compared with Testyolk buffer. Paper-7639162.
To identify host genomic sequences at the coagulation Factor X transgene integration site, DNA from a tail snip of the transgenic mouse was digested with NcoI and circularized using T4 DNA ligase. Paper-12976222.
In this report, we show that recombinant (125)I-ixolaris interacts with rat and human FX in plasma and prolongs the prothrombin time (PT) and activated partial thromboplastin time (aPTT) in vitro. Paper-12134366.
Temperature-sensitive mutants of vaccinia virus, with genetic changes that map to the open reading frame encoding the F10 protein kinase, exhibit a defect at an early stage of viral morphogenesis. Paper-10208119.
This vector was transferred to B16 ( F10) cells and recombinant angioarrestin secreted from the transfected cells was tested for anti-angiogenic activity using endothelial cell proliferation assay. Paper-11463814.
Restriction enzyme analysis placed the phi X gene A protein cleavage site in St-1 RF DNA in the HinfI restriction DNA fragment F10 and in the overlapping HaeIII restriction DNA fragment Z7. Paper-3527565.
Coagulation factor X is a serine protease containing three noncatalytic domains: an N-terminal gamma-carboxyglutamic acid (Gla)1 domain followed by two epidermal growth factor (EGF)-like domains. Paper-675872.
Compared with control subjects, no significant differences were observed in MRR30 or F30/50, whereas a higher value for F10/50 was measured (48% +/- 1% critical illness vs 40% +/- 1% control subjects, p < .01). Paper-851603.
In previous research we studied the cytotoxic effect of new Pt mercaptopyridine (MP) complexes on several tumoral cell lines ( F10, Föhn, LoVo and HeLa) as well as on the fibroblast cell line (3T3). Paper-1002621.
The objective of this study was to characterize the pharmacokinetics of a PCC (Beriplex P/N) containing coagulation factors II (FII), VII (FVII), IX (FIX) and X ( FX) and anticoagulant proteins C and S. Paper-12575763.
Activation of coagulation factor X (fX) by activated factors IX (fIXa) and VIII (fVIIIa) requires the assembly of the enzyme-cofactor-substrate fIXa-fVIIIa-fX complex on negatively charged phospholipid membranes. Paper-10829981.
In vivo, global down-regulation of vitamin K-dependent zymogens by warfarin significantly diminished liver uptake of CAR-deleted Ads; however, this phenomenon was fully rescued by acute infusion of human FX. Paper-12256137.
Surprisingly, Ham's F10 with no buffering component or with both NaHCO3 and N-Z-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) maintained sperm motility at levels similar (P > or = 0.05) to PBS. Paper-884175.
Mice given a similar treatment regimen of rHuIFN-alphaA/D had elevated natural killer (NK) cell activity as measured by in vitro cytotoxicity against YAC-I or in vivo pulmonary clearance of B16 F10 cells. Paper-4646369.
Tissue factor was detected on the cell surface of cultured human brain pericytes by immunocytochemistry and was shown to form a functional complex with factor (F) VIIa to effect both FIX and FX activation. Paper-873487.
To further study the role of the enzyme, we constructed recombinant vaccinia virus vF10V5i, which expresses inducible V5 epitope-tagged F10 and is dependent on a chemical inducer for plaque formation and replication. Paper-10208119.
Double immunofluorescence staining with F10 antibody and anti-ATLA-positive human serum caused co-capping on cell surfaces, which suggests that gp21 co-exists with other ATLV antigens expressed on cell surfaces. Paper-4700661.
We suggest that F10 may be useful for the identification of a novel epitope that is commonly shared by all trophoblasts and leukocytes and such an epitope may be potentially active in maternal-fetal interactions. Paper-490454.
Increased platelet count, F VII and FX activities and D-Dimer levels in patients with PHPT represent a potential hypercoagulable state, which might augment the risk for atherosclerotic and atherothrombotic complications. Paper-13074623.
Optimal cell viability and growth were achieved using Ham's F10 medium enriched with 20% fetal bovine serum, although cells from a patient with acromegaly also grew in serum-free, defined, hormone-containing medium. Paper-4223107.
Among tumor cell products or activities which may promote clot formation, cancer procoagulant (CP), a direct activator of coagulation factor X, has been suggested to be selectively associated with the malignant phenotype. Paper-5413802.
We have compared hormone production by early gestation and term human placental trophoblasts cultured in Ham's F10 medium containing 10% fetal bovine serum with that by cells cultured in serum-free HB102 medium. Paper-6787327.
In a previous study we characterized cancer procoagulant (CP), a 68 kd cysteine proteinase which directly activates coagulation factor X in various subtypes (from M1 to M5) of acute non-lymphoblastic leukemia ( ANLL). Paper-6599776.
In the present work we characterized the interaction of Ixolaris with human FX quantitatively, and identified a precursor state of the heparin-binding exosite (proexosite, HBPE) as the Ixolaris-binding site on the zymogen. Paper-12669683.
We show here that recombining the N-terminal subdomain from coagulation factor X with the C-terminal subdomain from trypsin creates a potent enzyme (fXYa) with novel properties, in particular a broad substrate specificity. Paper-1547278.
The haplotypes of FX alleles were determined by the following allelic variants located in the promoter: g.1323_1330delTTGTGA (A1/A2), g.1449T>C, g.1451C>A, upstream to exon 3: g.17257C>T and downstream to exon 3: g.17396A>C. Paper-13183974.
We studied B16 murine