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The presence of LCK in B cells was confirmed by western blotting. Paper-12123522.
One hepatic metastasis expressed more LCK message than the primary lesion. Paper-709538.
CONCLUSION: LCK is reduced in T cell lipid rafts from patients with SLE. Paper-9660158.
Human tonsillar B-cell subsets were analyzed by flow cytometry for LCK expression. Paper-12123522.
Tyrosine phosphorylation was dependent on expression of LCK in lipid rafts. Paper-2167748.
Our results indicate that the LCK message is strongly expressed in some colorectal cancers. Paper-709538.
The developed method is applied toward the QM docking study for the p56 LCK SH2 domain. Paper-13534292.
Chromosomal translocations joining LCK and TCRB loci in human T cell leukemia. Paper-42479.
We have analyzed LCK gene expression in HSB-2 and SUP- T12 cell lines. Paper-128314.
Oncogenes common to both lymphomas are MAFB, MET, NF-kappaB2, LCK, and LYN. Paper-11268469.
The lymphocyte-specific protein-tyrosine kinase Lck plays a critical role in T cell activation. Paper-9160988.
These results suggest that mutation of LCK may contribute to some human T-cell leukemias. Paper-116214.
The LCK gene codes for p56lck, a member of the SRC family of cytoplasmic tyrosine protein kinases. Paper-51985.
Both LCK-positive and BAFF-R-positive DLBCL tended to be of germinal-center phenotype. Paper-12123522.
T cell phenotype and levels of intracellular LCK were determined by flow cytometry. Paper-9660158.
P56lck A lymphocyte specific protein tyrosine kinase: activation, regulation and signal transduction. Paper-7908282.
In humans, the LCK gene is located at the breakpoint of the t(1;7)(p34;q34) chromosomal translocation. Paper-116214.
These results indicate that the LCK is a novel risk gene for AD regardless of the APOE genotype. Paper-11105110.
Lymphocyte activation provokes modification of a lymphocyte-specific protein tyrosine kinase (p56lck). Paper-6193505.
This study also indicated that the rat genome contains 2 LCK genes, unlike the human and murine genomes. Paper-6672210.
The differential expression of LCK and BAFF-receptor and their role in apoptosis in human lymphomas. Paper-12123522.
Augmented expression of LCK message directed from the downstream promoter in human colorectal cancer specimens. Paper-709538.
The structure-activity relationship of the series of non-peptide small antagonists for p56lck SH2 domain. Paper-13210087.
Sorafenib inhibits activation of human peripheral blood T cells by targeting LCK phosphorylation. Paper-12826077.
RESULTS: LCK was detected for the first time in germinal centers and, at lower levels, in mantle zone B cells. Paper-12123522.
The LCK gene encodes a lymphocyte-specific member of the Src family of protein tyrosine kinases. Paper-129228.
Despite the absence of LCK or FYN, IL-2 induced similar patterns of rapid tyrosine phosphorylation. Paper-79425.
Further Western analysis demonstrated that the zinc binding protein LCK was elevated in these zinc-deficient mice. Paper-8878926.
Molecular analysis of the T-cell acute lymphoblastic leukemia-associated t(1;7)(p34;q34) that fuses LCK and TCRB. Paper-128314.
Indeed, crosslinking of CD28 increases the activity of the intracellular tyrosine kinases EMT/ ITK and LCK. Paper-1410847.
The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in T cell maturation and signaling. Paper-9157588.
We selected five genes ( DUSP6, MMD, STAT1, ERBB3, and LCK) for RT-PCR and decision-tree analysis. Paper-12393144.
Furthermore, T cells allowed to "rest" in vitro showed a reversal of the changes in LCK, CD45, and GM1 expression. Paper-10404761.
Evidence for the involvement of LCK and MAP kinase ( ERK-1) in the signal transduction mechanism of interleukin-15. Paper-987568.
Increased ubiquitination and reduced expression of LCK in T lymphocytes from patients with systemic lupus erythematosus. Paper-9660158.
The IC50 for LCK inhibition by imatinib was 0.6 microM to 0.8 microM in an in vitro tyrosine kinase assay. Paper-11501766.
Functional LCK Is required for optimal CD28- mediated activation of the TEC family tyrosine kinase EMT/ ITK. Paper-550906.
Oncogenic activation of the Lck protein accompanies translocation of the LCK gene in the human HSB2 T-cell leukemia. Paper-116214.
Association of the tyrosine kinase LCK with phospholipase C-gamma 1 after stimulation of the T cell antigen receptor. Paper-7483045.
Moreover, DNA fragmentation was also induced by TBT in cells deficient in expression of p56lck, ZAP-70 and SHP-1. Paper-12710981.
The LCK gene is involved in the t(1;7)(p34;q34) in the T-cell acute lymphoblastic leukemia derived cell line, HSB-2. Paper-51985.
The breakpoint in SUP- T12 occurred 2 kb 5' of the type II promoter, leaving an intact LCK gene on the der(1) chromosome. Paper-128314.
The results revealed an increase in protein ubiquitination, and specifically LCK ubiquitination, in T cells from SLE patients. Paper-9660158.
All four hydroxyl groups of RosA were essential for binding with the p56lck SH2 domain and T-cell inhibitory activity. Paper-13210087.
These findings suggest that sorafenib may cause the loss of T-cell immune response by inducing apoptosis and targeting LCK. Paper-12826077.
A member of the src gene family, the lymphocyte-specific protein tyrosine kinase, p56lck, has been implicated in IL-2 production. Paper-7483219.
The T cell specific protein-tyrosine kinase ( PTK), p56lck, has been implicated in the tyrosine phosphorylation of TCR-zeta. Paper-6801390.
LCK B+7- mRNA is detected in all tested peripheral blood T lymphocytes total RNA samples but is apparently sequestered in the nucleus. Paper-11125908.
cDNA array analysis identifies thymic LCK as upregulated in moderate murine zinc deficiency before T-lymphocyte population changes. Paper-8878926.
Tyrosine phosphorylation-dependent activation of NF-kappa B. Requirement for p56 LCK and ZAP-70 protein tyrosine kinases. Paper-8724608.
Analysis of CD28 cytoplasmic tail tyrosine residues as regulators and substrates for the protein tyrosine kinases, EMT and LCK. Paper-851163.
Furthermore, co-transfection of LCK and EMT into COS-7 cells showed that EMT becomes phosphorylated in the presence of LCK. Paper-550906.
Absence of transcription of lck ( lymphocyte specific protein tyrosine kinase) message in IL-2-independent, HTLV-I-transformed T cell lines. Paper-6197264.
The EMT/ ITK/ TSK ( EMT) tyrosine kinase is activated during TCR signaling: LCK is required for optimal activation of EMT. Paper-524647.
The functional significance of p56-LCK kinase activation for IL-2-mediated lymphocyte responses, however, has never been directly assessed. Paper-84661.
Lymphocyte-specific protein tyrosine kinase (Lck) inhibitors have treatment potential for autoimmune diseases and transplant rejection. Paper-13736153.
Localization of a lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosomal abnormalities in human lymphomas. Paper-5434851.
To identify the likely causes of reduced LCK, we explored the possibility that chronic activation of T lymphocytes underlies LCK degradation. Paper-9660158.
We suggest that the CD4-associated lymphocyte-specific protein tyrosine kinase p56lck may be involved in preventing CD4 endocytosis in T cells. Paper-6887793.
Lead validation and SAR development via chemical similarity searching; application to compounds targeting the pY+3 site of the SH2 domain of p56lck. Paper-11794659.
However, in this report, we now demonstrate that neither LCK nor FYN are obligatory for IL-2-induced growth of HTLV-I-infected human T cells. Paper-79425.
We demonstrate that EMT can phosphorylate all four tyrosines of the CD28 tail, in contrast to LCK, which phosphorylates only tyrosine 173. Paper-851163.
CA125 did not reduce proliferation or induce apoptosis of NK cells and alter the expression of p56lck, phospholipase Cgamma1, ZAP70, or CD3zeta. Paper-11467269.
Gene transfer investigations of p56-LCK function in IL-2-dependent T-cell lines: implications for mechanisms of IL-2-signal transduction. Paper-84661.
Somatic cell mutants of the Jurkat T cell line, which lack the SRC family kinase LCK (JCaM1.6), fail to produce IL-2 when stimulated through the TCR complex. Paper-524647.
Engagement of the CD19 receptor on human B-lineage leukemia cells activates LCK tyrosine kinase and facilitates radiation-induced apoptosis. Paper-7878071.
Interleukin 4 inhibits IL-2- induced proliferation of a human T-leukemia cell line without interfering with p56-LCK kinase activation. Paper-74160.
DX-induced ZAP-70 phosphorylation did not occur in the absence of active p56-lck as examined in the p56-lck kinase-deficient Jurkat cell line JCaM1. Paper-10825382.
Proteins in the lipid raft and nonraft fractions were analyzed by Western blotting and probed for phosphotyrosine activity and for LCK, LAT, and CD3 epsilon. Paper-9660158.
The effect of p56lck, a lymphocyte specific protein tyrosine kinase, on the syncytium formation induced by human immunodeficiency virus envelope glycoprotein. Paper-7457560.
The lymphocyte-specific protein tyrosine kinase pp56lck, encoded by a member of the src gene family, is implicated in the control of T-cell growth and differentiation. Paper-5732452.
Taken together, the data indicate that the EMT tyrosine kinase is activated following cross-linking of the TCR, a process in which LCK likely plays an important role. Paper-524647.
Ligation of CD4 by zanolimumab effectively inhibits early TCR signaling events but, interestingly, activates signaling through the CD4-associated tyrosine kinase p56lck. Paper-12526396.
Efficient CD28 signalling leads to increases in the kinase activities of the TEC family tyrosine kinase EMT/ ITK/ TSK and the SRC family tyrosine kinase LCK. Paper-1410847.
Using gene transfer approaches, we have achieved markedly elevated levels of p56-LCK kinase activity in the IL-2-dependent cytolytic T-cell line CTLL-2 and the helper line HT-2. Paper-84661.
In addition, increases in EMT association with CD28 were eliminated in a LCK-negative Jurkat cell line, but were restored following transfection of wild type LCK. Paper-550906.
Resequencing of LCK in 32 individuals detected seven single nucleotide polymorphisms ( SNPs) with allele frequencies >3%, including four common SNPs previously reported. Paper-10538045.
In contrast, the distal three tyrosines in CD28 are required for optimal IL2 production as well as for optimal activation of the LCK and EMT/ ITK tyrosine kinases. Paper-1410847.
INTERPRETATION AND CONCLUSIONS: The reciprocal expression pattern of LCK and BAFF-R in germinal center and mantle zone B cells may reflect their opposing roles in apoptosis. Paper-12123522.
The lymphocyte-specific protein tyrosine kinase p56lck is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD2. Paper-6487407.
These results also demonstrate changes in the function and localization of critical signalling molecules such as the LCK tyrosine kinase and the CD45 tyrosine phosphatase. Paper-10500299.
The Lin(-)Sca1(+)cKit(+) ( LSK) compartment is expanded and "right-shifted," accompanied by increased stem cell factor (SCF)-evoked colony formation and Erk and Akt activation. Paper-13744890.
Human HCK, C-SRC (encoding p60 Src PTK), C-FGR and LCK (encoding p56 Lck, PTK) genes thus share very similar exon/ intron structures for the conserved exons. Paper-7509088.
LCK transcription is regulated from two distinct promoters and initiated exclusively from the downstream promoter in colon carcinoma cell lines in contrast to peripheral lymphocytes. Paper-709538.
Fc gamma RIIA- mediated signal transduction was defective in a transfected mutant T-cell line exhibiting reduced expression of the tyrosine kinases LCK and FYN. Paper-135120.
CD45 tyrosine phosphatase, which regulates LCK activity, was differentially expressed and its localization into lipid rafts was increased in T cells from patients with SLE. Paper-10404761.
Using these systems, we show that KIR CYT, once phosphorylated by the src-family tyrosine kinase LCK, additionally bind the p85alpha regulatory subunit of phosphatidylinositol (PI) 3-kinase. Paper-1587757.
The T cell-specific protein tyrosine kinase ( PTK) ZAP-70 is believed to play a key role in early tyrosine phosphorylations of cellular proteins induced by CD3 stimulation. Paper-784172.
In support of a role for LCK in EMT activation, reconstitution of the LCK-negative Jurkat T cell line by enforced expression of LCK restored TCR- mediated EMT activation. Paper-524647.
By restriction mapping, Southern blot analysis, and DNA sequencing we showed that the translocation breakpoint on chromosome I is located within the first intron of the LCK gene. Paper-51985.
The lymphocyte-specific protein-tyrosine kinase p56lck has been purified 90-fold to approximately 30% purity in 30% yield from a baculovirus expression system by a two-column purification procedure. Paper-7194578.
To assess the in vivo role of B-myb, we investigated the phenotype of mouse transgenic lines in which B-Myb expression in lymphoid tissues was driven by the LCK proximal promoter. Paper-8995096.
Overall results revealed important structural requirements of the p56lck SH2 antagonists for in vitro T-cell inhibitory activity and in vitro protein binding activity. Paper-13210087.
These results, therefore, suggested that nocodazole prevented initial phosphorylation of the TCR by LCK after stimulation, and as a result, it blocked activation of downstream signaling pathways. Paper-1439764.
LCK is required for the activation and phosphorylation of EMT induced by ligation of the TCR or CD28 placing LCK upstream of EMT in T cell signaling cascades. Paper-1638489.
A mechanism for the inhibition of TCR signaling by nocodazole was suggested by in vitro assays, which revealed that the drug inhibited the kinase activity of LCK and, to a lesser extent, FYN. Paper-1439764.
We applied this technique to the analysis of in vitro sites of tyrosine phosphorylation induced on the T cell-specific protein tyrosine kinase ZAP-70 in the absence and presence of p56lck. Paper-8005653.
Increased levels of phosphotyrosine in HSB2 cells resulted from mutations in the LCK gene that activated its function as a phosphotransferase and converted it into a dominant transforming oncogene. Paper-116214.
With the use of different Jurkat cell mutants it was demonstrated that CD2- mediated activation of EMT required expression of LCK, but not require surface expression of the CD3 zeta chain. Paper-882356.
A rare mRNA variant of the human lymphocyte-specific protein tyrosine kinase LCK gene with intron B retention and exon 7 skipping encodes a putative protein with altered SH3-dependent molecular interactions. Paper-11125908.
A non-myristylated form ( LCK M) of the human T-lymphocyte-specific protein tyrosine kinase ( LCK) was produced at high levels in a baculovirus expression system ( BVES) using two strategies. Paper-562769.
The promoter methylation status of three of these down-regulated genes ( CD10, CD19, and LCK) was further studied in microdissected L&H cells, and this revealed that their promoters were unmethylated. Paper-12604750.
Previously, we identified rosmarinic acid (alpha-o-caffeoyl-3,4-dihydroxyphenyl-lactic acid; RosA) from Prunella vulgaris as an antagonist for the p56lck SH2 domain by screening natural products. Paper-13210087.
In support of a role for LCK in EMT activation, reconstitution of a LCK-negative Jurkat T cell line by transfection with normal LCK recreates CD28- mediated EMT activation. Paper-550906.
We demonstrate herein that murine Y170 (equivalent to human Y173) in CD28 is also dispensable for activation of the SRC family tyrosine kinase LCK and the TEC family tyrosine kinase EMT/ ITK. Paper-1410847.
Mutant T cell lines contained a more active pool of p56lck tyrosine kinase and responded with increased phosphorylation of Zap70 and TCR-zeta and an enhanced Ca2+ flux to TCR/CD3 stimulation. Paper-10824006.
BACKGROUND AND OBJECTIVES: We explored the expression of LCK and BAFF-R ( B-cell activating factor receptor) both of which are known to play a role in signaling and apoptosis, in routine tissue biopsies. Paper-12123522.
The presence of intron B sequence does not disrupt the reading frame and results in the insertion of 58 aminoacids, containing a proline-rich region just upstream of p56lck SH3 domain. Paper-11125908.
Most lymphomas of germinal center origin (e.g. follicular lymphoma) and also many mantle cell lymphomas, chronic lymphocytic leukemia (CLL) and most T-cell neoplasms expressed LCK. Paper-12123522.
Localization of Zap70, the gene for a T cell-specific protein tyrosine kinase, to mouse and rat chromosomes by fluorescence in situ hybridization and molecular genetic linkage analyses. Paper-900319.
The enzymatic properties of the re-proteins and their inhibition by protein kinase inhibitors were comparable to the native enzyme ( LCK N) derived from Jurkat cells and wild-type LCK derived from the BVES. Paper-562769.
Interaction of the interleukin 2 receptor (IL-2R) beta chain with the lymphocyte-specific protein tyrosine kinase ( PTK), p56lck, has led to the speculation that p56lck participates in growth signal transduction. Paper-159099.
ZAP-70 becomes phosphorylated and activated by LCK protein tyrosine kinase after interaction of its two NH2-terminal SH2 domains with tyrosine-phosphorylated subunits of the activated TCR. Paper-1069856.
Ligation of the CD2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src kinases, LCK and FYN. Paper-882356.
This translocation fused the beta T-cell receptor gene ( TCRB) constant region and transcriptional enhancer with the type I transcription unit of the LCK gene on the derivative 1 [der(1)] chromosome. Paper-128314.
There are two classes of LCK transcripts (type I and type II), each expressed from a distinct promoter, and each having a unique 5' untranslated region (UTR); the protein coding regions of the two classes are identical. Paper-51985.
A heptamer-nonamer recognition sequence with a 12-bp spacer was found in the immediate vicinity of the 1p34/ LCK breakpoint and, thus, chromosomal breakage at 1p34 may be explained as resulting from recombinase activity. Paper-128314.
The frequency and intensity of TCR-CD3zeta chain, p56lck, p59fyn, ZAP-70, PI3-kinase and interferon (IFN)-gamma/interleukin (IL)-4 production in CD4 and CD8 T cells was examined by flow cytometry. Paper-12186426.
Activation of the IL-2R leads to serine and threonine phosphorylation of the SRC tyrosine kinase family member, LCK, and an increase in LCK tyrosine kinase activity. Paper-79425.
We previously described that lymphocyte-specific protein tyrosine kinase ( LCK) and FYN oncogene related to SRC, FGR, YES ( FYN) mediate GC-induced inhibition of T-cell-receptor (TCR) signalling. Paper-12251503.
RESULTS: C77G individuals showed an increased proportion of primed CD45RA and effector memory CD8 T cells and more rapid activation of the lymphocyte specific protein tyrosine kinase ( Lck) following CD3 stimulation. Paper-12161363.
Utilizing somatic cell mutants lacking LCK, we demonstrate that functional LCK is required for CD28- induced activation of EMT as evidenced by increased tyrosine phosphorylation and kinase activity. Paper-550906.
This IL-2- mediated increase in LCK kinase activity was manifested both by increased kinase autophosphorylation and by increased phosphorylation of the exogenous substrate enolase during in vitro kinase assays. Paper-74160.
In primary tumors and TCL1-transfected T-cell lines, TCR engagement leads to rapid recruitment of TCL1 and AKT to transient membrane activation complexes that include TCR-associated tyrosine kinases, including LCK. Paper-12668004.
The breakpoint in the t(1;7) separates the two LCK promoters and juxtaposes the constant region of the TCR beta locus with the proximal promoter and with the protein-coding region of the LCK gene on the der(I) chromosome. Paper-51985.
Finally, the intracellular regions of both CD4 and CD4REL possess the canonical CXC motif involved in the interaction of CD4 with p56LCK, implying that similar mechanisms for CD4(+) T cell activation are present in all vertebrates. Paper-12207262.
p56Lck and p59Fyn regulate CD28 binding to phosphatidylinositol 3-kinase, growth factor receptor-bound protein GRB-2, and T cell-specific protein-tyrosine kinase ITK: implications for T-cell costimulation. Paper-357268.
This tyrosine phosphorylation of p72ITK/ EMT is rapid (within 30 sec), occurs in the absence of LCK activation, and precedes tyrosine phosphorylation of the guanine nucleotide exchange factor VAV. Paper-133248.
To investigate the oncogenic potential of HOX11, we targeted its expression in lymphocytes of transgenic mice by placing the human cellular DNA under the transcriptional control of Ig heavy chain or LCK regulatory sequences. Paper-1649385.
We have previously shown that p56lck, a lymphocyte-specific protein tyrosine kinase, is hyperphosphorylated on serine and tyrosine residues 15 minutes after activation via CD2 with a concomitant shift to a higher molecular mass. Paper-7196152.
A rare mRNA variant of the human lymphocyte-specific protein tyrosine kinase LCK gene that retains intron B and excludes exon 7 (B+7-) due to alternative splicing of the canonical LCK transcripts was identified and characterized. Paper-11125908.
To investigate the pathogenesis of pX gene in lymphoid tissues, we established a series of novel transgenic rats carrying the pX gene under the control of a rat lymphocyte-specific protein tyrosine kinase (p56lck) proximal promoter. Paper-9625546.
Among the genes identified are uroguanylin (UG), cholecystokinin, lymphocyte-specific protein tyrosine kinase ( LCK), T-cell cytokine receptor, heat shock proteins and the DNA damage repair and recombination protein-23B. Paper-9691437.
Sequence comparisons of all 90 tyrosine kinase genes in the human genome for homology in the adenosine triphosphate ( ATP) binding pocket identified LCK, which is required for ZAP70 activation, as a likely target for imatinib. Paper-11501766.
Here, effects of anti-CD3 and anti-CD28 antibodies on Kv1.3 current were examined in three types of human T lymphocyte derived cell lines, Jurkat E6-1, p56lck-kinase deficient mutant JCaM.1, and CD45-phosphatase deficient mutant J45.01. Paper-12890177.
To investigate the structure-activity relationship of RosA and to identify a novel p56lck SH2 antagonist with more potent in vitro T-cell inhibitory activity, we synthesized several analogs of RosA by using rational design. Paper-13210087.
Furthermore in Jurkat T cell extracts, a recombinant intron B plus SH3 p56lck domain fails to interact with some TCR-induced tyrosine phosphorylated polypeptides and known p56lck partners such as Sam68 and c-Cbl. Paper-11125908.
Lymphocyte-specific protein tyrosine kinase ( LCK) is a lymphoid-specific, Src family protein tyrosine kinase that is known to play a pivotal role in T-cell activation and interact with the T-cell coreceptors, CD4 and CD8. Paper-11105110.
Our results indicate that the expression of raft-associated ganglioside, GM1, is increased in T cells from SLE patients and LCK may be differentially regulated due to an alteration in the association of CD45 with lipid raft domains. Paper-10404761.
EMT activation, as evidenced by increased EMT tyrosine phosphorylation and EMT-associated kinase activity, was also greatly reduced following stimulation of the TCR in the JCaM1.6 Jurkat T cell mutants that lack LCK. Paper-524647.
TSAd is required for normal TCR- induced synthesis of IL-2 and other cytokines in T cells and acts at least in part by promoting activation of the LCK protein tyrosine kinase at the outset of the TCR signaling cascade. Paper-12934795.
Thus, our results indicate a mechanism whereby TCR engagement promotes aggregation of lipid rafts, which facilitates colocalization of LCK, LAT, and the TCR whilst excluding CD45, thereby triggering protein tyrosine phosphorylation. Paper-2025755.
In the present paper, we report an extensive phenotypic analysis of L&H cells which revealed down-regulation of a number of markers associated with the B-cell lineage (eg CD19, CD37) and with the germinal centre maturation stage (eg PAG, LCK). Paper-12604750.
We investigated the expression of the downstream promoter-initiated LCK transcript in 18 colorectal primary cancer and normal mucosae, and two hepatic metastases, using a RNase protection assay with the EcoRI-BglII fragment of human LCK cDNA, YT16. Paper-709538.
In contrast to IL-2's effects on p56-LCK in T-cells, studies of an IL-2-responsive cell line of the B-cell lineage that lacks p56-LCK revealed that IL-2 specifically regulates the activity of the p53/56-LYN kinase. Paper-62284.
We examined the relationship between AD and the LCK and apolipoprotein E ( APOE) genes in 376 AD (including 323 late-onset AD (LOAD) cases and 53 early-onset AD (EOAD) cases) and 378 non-demented controls using a single nucleotide polymorphism (SNP). Paper-11105110.
IVMP significantly reduced mRNA levels for T-cell-specific transcription factor 7 (p=0.02), T-cell-specific protein-tyrosine kinase (p=0.02), T-cell surface glycoprotein CD5 (p=0.05) and interferon-stimulated gene factor 3 gamma subunit (p=0.04). Paper-10561618.
This translocation positions the beta T-cell receptor constant region enhancer upstream of the LCK gene without interrupting the LCK coding sequences, and a translocation of this sort occurs in both the HSB2 and the SUP-T-12 T-cell lines. Paper-116214.
RESULTS: LCK, an essential signaling molecule for T cell activation, was significantly reduced in both lipid raft and nonraft fractions of T lymphocytes from patients with active SLE compared with controls, and the reduction was independent of treatment. Paper-9660158.
In contrast, a strong positive prognostic value for the T-cell surrogate marker (lymphocyte-specific kinase ( LCK) metagene) was observed among all estrogen receptor (ER)-negative tumors and those ER-positive tumors with a HER2 overexpression. Paper-13814100.
METHODS: In this retrospective study, the records of 34,099 eyes of 17,388 patients who underwent LASIK for myopia, hyperopia, and astigmatism using the Moria LSK One manual microkeratome and the Bausch & Lomb Technolas 217 Z excimer laser were reviewed. Paper-13319394.
We show that tcasp8-/- mice develop an age-dependent lethal lymphoproliferative and lymphoinfiltrative immune disorder characterized by lymphoadenopathy, splenomegaly, and accumulation of T cell infiltrates in the lungs, liver, and kidneys. Paper-10798138.
These findings thus imply the existence of other signal-transducing molecules, besides p56-LCK, that physically participate in IL-2R complexes and that are necessary for initiation of the biochemical events ultimately responsible for IL-2's pleiotropic actions on lymphocytes. Paper-84661.
Three protein tyrosine kinases, i.e., LCK, LYN, and SYK, were activated by occupancy of the Fc gamma RIIIA, and only LCK activity showed a divergence in effects induced by the various ligands, with strong autophosphorylation induced by mIgG upon cross-linking. Paper-463276.
CD40-resistant lines expressed pre-B-cell markers, including RAG and VPREB, whereas CD40-sensitive cells resembled mature B cells and expressed higher levels of transcripts encoding several members of the CD40 signaling pathway, including LCK and VAV. Paper-11395136.
Taken together, these data indicate that while IL-2 can up-regulate the enzymatic activity of p56-LCK, elevated levels of p56-LCK tyrosine kinase activity are insufficient to stimulate IL-2-mediated pathways required for T-cell growth and survival. Paper-84661.
Expression of one of these genes ( myeloid cell leukemia sequence-1) was depressed, whereas the others [ DNA damage repair and recombination protein 23B, the mouse laminin receptor and the lymphocyte-specific protein tyrosine kinase ( LCK)] were elevated in the zinc-deficient mice. Paper-8878926.
In TALL-103/2 cells, IL-2 stimulated concentration-dependent increases in p56-LCK activity that displayed rapid and transient kinetics: detectable increases occurred within 1 minute after IL-2 stimulation, peaked at 10 minutes, and declined to baseline levels by 30 minutes. Paper-74160.
Because phosphorylation of Tyr-505 in vivo regulates the tyrosine kinase activity of p56lck we amplified a region from LCK exon 12 that contains the codon for Tyr-505 and showed no mutation of this codon in HSB-2 DNA and, therefore, p56lck in HSB-2 is not activated by mutation of Tyr-505. Paper-128314.
Through genetic dissection strategies, we have identified that the acquired immune system is activated through the T cell receptor and signaling amplification systems, such as the tyrosine kinase p56lck, phosphatase CD45 and downstream ERK1/2, and the family of cytokines. Paper-10831890.
The introduction of a mutated cysteine motif in CD8alpha, which prevents its binding to LCK and linker for activation of T cells, did not adversely affect expression and T cell cytotoxicity, but counteracted the CD8alpha-mediated down-regulation of IL-4 and IL-5, but not IL-10. Paper-12057084.
Recently, the SRC-like non-receptor protein tyrosine kinase p56- LCK has been shown to physically associate with the interleukin-2 receptor ( IL-2-R) complex and to undergo rapid elevations in its tyrosine kinase activity upon stimulation of T lymphocytes with IL-2. Paper-84661.
Taken together, the tyrosine phosphorylation and presumably kinase activity of p56lck were swiftly enhanced by oxidative stress, indicating that T cells have a redox-sensitive signaling mechanism, which is partly mediated by the lymphocyte-specific protein tyrosine kinase p56lck. Paper-7872736.
Prior data associating the expression of lymphocyte-specific protein tyrosine kinase ( LCK) with type 1 diabetes, its critical function in lymphocytes, and the linkage of the region to diabetes in the nonobese diabetic (NOD) mouse model make LCK a premier candidate for a susceptibility gene. Paper-10538045.
Our previous studies have established that lymphocyte-specific protein tyrosine kinase ( LCK) is reduced in T lymphocytes from patients with SLE and that this reduction is associated with disease activity and parallels an increase in LCK ubiquitination independent of T cell activation. Paper-10404761.
HSCs can efficiently initiate apoptosis after activation of the SAC in LSK cells as indicated by increased hypodiploidy and increased levels of activated caspase 3, suggesting that HSCs behave more like somatic cells instead of ESCs with respect to this important cell cycle checkpoint. Paper-13016906.
IGF-1 administration increased bone marrow LSK (lineage(-), Sca-1(+), c-kit(+)) precursor proliferation and peripheral LSK populations, increased thymocyte populations in a sequential wave of expansion, and proportionately expanded TEC subpopulations and enhanced their chemokine expression. Paper-12951328.
We earlier reported that Fischer 344/jcl strain (F344) rats carrying a unique pX gene of human T lymphocyte virus type I ( HTLV-I) under control of a rat lymphocyte-specific protein tyrosine kinase (p56lck) type I promoter (lck-pX rats) spontaneously developed epithelial thymomas from the thymic medulla. Paper-10365536.
We have previously reported that lck mRNA (a lymphocyte-specific protein tyrosine kinase gene) is absent in human T-cell leukemia virus type I (HTLV-I)-infected interleukin-2(IL-2)-independent T-cell lines, while HTLV-I-negative T-cell lines and HTLV-I-positive IL-2-dependent ones express a large amount of lck mRNA. Paper-6819445.
Furthermore, when cultured in the absence of IL-2, transfected T cells whose relative levels of p56-LCK activity were elevated by approximately 20-50-fold died with the same kinetics as control cells and underwent apoptosis, as defined by uptake of trypan blue dye and DNA fragmentation assays, respectively. Paper-84661.
Apoptosis of antigen-specific T lymphocytes upon the engagement of CD8 by soluble HLA class I molecules is Fas ligand/Fas mediated: evidence for the involvement of p56lck, calcium calmodulin kinase II, and Calcium-independent protein kinase C signaling pathways and for NF-kappaB and NF-AT nuclear translocation. Paper-11492228.
The expression of lck gene ( lymphocyte specific protein tyrosine kinase) in the human system was examined by Northern blot analysis in human T cell leukemia virus type I (HTLV-I)-positive T cell lines, HTLV-I-T cell lines, normal T cell population, and a T cell line infected with human immunodeficiency virus. Paper-6197264.
CD28 interacts with three intracellular proteins-phosphatidylinositol 3-kinase ( PI 3-kinase), T cell-specific protein-tyrosine kinase ITK (formerly TSK or EMT), and the complex between growth factor receptor-bound protein 2 and son of sevenless guanine nucleotide exchange protein (GRB-2-SOS). Paper-357268.
In conclusion, CD8alpha down-regulates the production of major Th2-type cytokines, in part mediated by LCK and/or linker for activation of T cells, and may induce differentiation of tumor-specific Th1 cells, which makes this coreceptor an interesting candidate to improve the clinical potential of TCR gene transfer to treat cancer. Paper-12057084.
In addition, sorafenib induced T-cell apoptosis at concentrations higher than 10 muM. sorafenib also caused G(0)/G(1) phase arrest, inhibition of CD25 and CD69 expression, interleukin-2 production and LCK phosphorylation in the T cells; all of these effects exhibited dose and time dependence. Paper-12826077.
The following loci were newly assigned by Southern hybridization to sheep Chr 2: lipoprotein lipase ( LPL), glycoprotein-4-beta galactosyltransferase 2 ( GGTB2), neurofilament light polypeptide (68 kDa; NEFL), surfactant-associated protein 2 ( SFTP2), lymphocyte-specific protein tyrosine kinase ( LCK), and nebulin ( NEB). Paper-739607.
Our results indicate that 1) activation of EMT is partially dependent upon tyrosine 173 of the CD28 tail, although it does not require PI3-kinase activation; 2) activation of LCK is independent of CD28 cytoplasmic tail tyrosine residues; and 3) elements sufficient for the activation of both kinases are contained within the first half of the tail. Paper-851163.
These synonyms are used for gene LCK (lymphocyte-specific protein tyrosine kinase): YT16, T cell-specific protein-tyrosine kinase, Proto-oncogene tyrosine-protein kinase LCK, pp58lck, p56-LCK, p56lck, Lymphocyte cell-specific protein-tyrosine kinase, LSK.
These accession numbers are used for gene LCK: Q7RTZ3 (UNIPROT__AC), Q13152 (UNIPROT__AC), CAA29667 (NCBI_GENBANK__AC), AAC50287 (NCBI_GENBANK__AC).
LCK is a homologue of zgc:136695 (zgc:136695) from Danio rerio.
LCK is a homologue of LCK (lymphocyte-specific protein tyrosine kinase) from Bos taurus.
LCK is a homologue of LCK (lymphocyte-specific protein tyrosine kinase) from Canis lupus familiaris.
LCK is a homologue of Lck (lymphocyte protein tyrosine kinase) from Mus musculus.
LCK is a homologue of Lck (lymphocyte-specific protein tyrosine kinase) from Rattus norvegicus.
LCK is a homologue of lck (lymphocyte-specific protein tyrosine kinase) from Danio rerio.
Important links !
iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.