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The estimated molecular weight of PLTP is 75,000. Paper-7672441.
The role of PLTP in atherogenesis is still controversial. Paper-13178030.
Another important function of PLTP is connected to lipolysis. Paper-11290931.
Phospholipid transfer protein restored biexponential kinetics. Paper-7874604.
In humans, high PLTP is associated with type II diabetes and obesity. Paper-10087751.
The activity of PLTP in type 2 diabetes is decreased by atorvastatin. Paper-11826400.
Plasma PLTP activity was not affected by either dose of atorvastatin. Paper-9480569.
These variants and wild-type (WT) PLTP were expressed in COS-7 cells. Paper-12151835.
Role of cysteine residues in human plasma phospholipid transfer protein. Paper-1878785.
Substrate specificity of human plasma phospholipid transfer protein. Paper-5027146.
Fibrate treatment did not influence PLTP mRNA levels in human hepatocytes. Paper-11174394.
Results: Simvastatin significantly increased CSF PLTP activity (p = 0.005). Paper-12309489.
Phospholipid transfer activities in toad oocytes and developing embryos. Paper-5769604.
Both the transfer and exchange activities of PTP are stimulated by lipolysis. Paper-4985052.
Phospholipid transfer between phosphatidylcholine-taurocholate mixed micelles. Paper-5910480.
Neutralization and transfer of lipopolysaccharide by phospholipid transfer protein. Paper-562794.
PLTP activity was higher in affected than unaffected family members (P = 0.025). Paper-9937708.
For PL liposomes, DMSO sharpened the transitions. Paper-11233063.
Plasma phospholipid transfer protein activity, a determinant of HDL kinetics in vivo. Paper-12329015.
Localization of the human phospholipid transfer protein gene to chromosome 20q12-q13.1. Paper-421917.
Influence of obesity and insulin sensitivity on phospholipid transfer protein activity. Paper-8909767.
Complete cDNA encoding human phospholipid transfer protein from human endothelial cells. Paper-115621.
Characterization of phospholipid transfer between mixed phospholipid-bile salt micelles. Paper-6671510.
Phospholipid transfer protein reduces phosphorylation of tau in human neuronal cells. Paper-13991547.
Nonvesicular phospholipid transfer between peroxisomes and the endoplasmic reticulum. Paper-13037294.
HA- PLTP was subjected to a second heparin-Sepharose step and hydroxylapatite chromatography. Paper-9161444.
Size-exclusion and heparin-affinity chromatography assessed the molecular form of PLTP. Paper-10804967.
A possible importance of PLTP-mediated cholesterol transfer in the circulation was described. Paper-950295.
Plasma PLTP was chemically modified using diethylpyrocarbonate or ethylmercurithiosalicylate. Paper-8562754.
Genetics and molecular biology: phospholipid transfer protein in atherogenesis. Paper-12309473.
Finally, we demonstrate that bile acids are able to regulate PLTP gene expression in vivo. Paper-8507843.
As a net result, treatment with PLTP increased the cholesterol efflux into total plasma by 40%. Paper-575565.
Plasma phospholipid transfer protein activity and small, dense LDL in type 2 diabetes mellitus. Paper-9736919.
IHC demonstrated PLTP presence in neurons, astrocytes, microglia, and oligodendroglia. Paper-9905027.
PLTP was secreted during both early and late time points of hMADS adipocyte differentiation. Paper-12892669.
We have determined the exon/ intron organization of the human phospholipid transfer protein gene. Paper-165961.
Neither HA- nor LA- PLTP enhanced cholesterol efflux to lipid-free apoA-I. Paper-12524026.
In contrast to spontaneous transfer, PLTP mediates the accumulation of PC in small rHDL particles. Paper-966073.
First, we found that ERAD and cell surface re-uptake were not active in PLTP-null hepatocytes. Paper-10734065.
The molecular weight of PLTP is 78,000 as estimated by SDS-PAGE and by gel filtration. Paper-338543.
Serum phospholipid transfer protein mass as a possible protective factor for coronary heart diseases. Paper-10305943.
With diet-induced weight loss (16 +/- 7.3 kg), PLTP activity significantly decreased 9.1% (P = 0.002). Paper-9973760.
Bezafibrate-induced change in PLTP activity correlated with change in FFAs (r = 0.455, P = 0.058). Paper-9937698.
Plasma phospholipid transfer protein activity and subclinical inflammation in type 2 diabetes mellitus. Paper-10802714.
OBJECTIVE: To investigate the association between PLTP genetic variants and obesity-related phenotypes. Paper-11233524.
The facilitated transfer reaction of alpha-tocopherol could be blocked by specific anti- PLTP antibodies. Paper-1836792.
Wheat lipid transfer protein ( LTP) Tri a 14 is a major allergen associated with wheat allergy. Paper-13894896.
DNA sequences essential for transcription of human phospholipid transfer protein gene in HepG2 cells. Paper-956527.
Splenic T cells from these mice proliferated in response to native beta2GPI, alone or bound to anionic PL. Paper-14036171.
The decrease in plasma PLTP activity after insulin was larger in B1B1 than in B2B2 homozygotes (P < 0.05). Paper-1977887.
Separation of a plasma phospholipid transfer protein from cholesterol ester/phospholipid exchange protein. Paper-4261668.
We have studied the biosynthesis and secretion of PLTP using a stably transfected inducible HeLa cell line. Paper-1654605.
However, PLTP activity correlated with components of VLDL and LDL and was influenced by the type of obesity. Paper-13120770.
CONCLUSION: Intronic variants of the PLTP gene are significantly associated with obesity-related phenotypes. Paper-11233524.
Our study suggests that PLTP may be an important modulator of signal transduction pathways in human neurons. Paper-13991547.
The PL molsp profile of an animal species is not determined by phylogeny or thermal behavior. Paper-11000022.
DNA sequences responsible for reduced promoter activity of human phospholipid transfer protein by fibrate. Paper-8320509.
Haptoglobin levels and PLTP activity are inversely proportional in hyperlipidemic plasmas (R2 = 0.57, p > 0.05). Paper-13944579.
PLTP is involved in the generation of pre beta-HDL that can act as initial acceptors of cellular cholesterol. Paper-8998347.
PLTP expression was observed in elicited mouse peritoneal macrophages and in cultured Raw264.7 cells as well. Paper-9937695.
Phospholipid transfer from the viral envelope to the lipid bilayer membrane occurred within 30 s at pH 4.5-5. Paper-3844782.
However, the PLTP activity level in the medium of cultured macrophages was determined by HDL levels in the medium. Paper-13894711.
No changes in the activities of phospholipid transfer protein and cholesterol ester transfer protein were evident. Paper-10394314.
However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. Paper-12524026.
Bezafibrate-induced diminished insulin resistance is associated with a reduction of CET and PLTP activity. Paper-9937698.
It is proposed that plasma PLTP mass levels are related to glucose metabolism rather than to lipid metabolism. Paper-1754515.
Expression of plasma phospholipid transfer protein mRNA in normal and emphysematous lungs and regulation by hypoxia. Paper-1469325.
CONCLUSIONS: The allergen involved in the lettuce-induced anaphylaxis of our patients was the LTP Lac s 1. Paper-13810095.
Phospholipid transfer protein from maize seedlings has been crystallized using trisodium citrate as precipitant. Paper-8034349.
The complete amino acid sequence phospholipid-transfer protein isolated from spinach leaves has been determined. Paper-5635466.
PLTP stimulates hepatic triglyceride secretion and reduces plasma levels of high density lipoproteins (HDL). Paper-12231007.
We have used the human hepatoma cell line, HepG2, as a model to study PLTP secreted from hepatic cells. Paper-9973751.
Consistent with these findings, PLTP activity in cerebrospinal fluid amounted to 23% +/- 3% of that in rabbit plasma. Paper-9408605.
Enhancement of phospholipid transfer from Sendai virus to erythrocytes is mediated by target cell membrane. Paper-3668952.
PLTP activity is elevated in diabetes mellitus (both type 1 and type 2), obesity and insulin resistance. Paper-9400876.
Using multivariate logistic regression analysis, PLTP activity was found to have independent predictive value for CAD. Paper-10087751.
Association of plasma phospholipid transfer protein activity with IDL and buoyant LDL: impact of gender and adiposity. Paper-9459661.
This study defines the mechanism of the remodeling of HDL by PLTP and determines why it is enhanced in TG-enriched HDL. Paper-9018969.
This finding suggests that high glucose upregulates the transcription of human PLTP gene via nuclear hormone receptors. Paper-8852119.
Here we report the molecular cloning of pig PLTP and the eukaryotic cell expression of its complementary DNA. Paper-1141552.
PLTP activity inversely correlated with average periodontal pocket depth (PPD) per tooth (r(s) = -0.372; P = 0.002). Paper-13017425.
Secretion of phospholipid transfer protein by human hepatoma cell line, Hep G2, is enhanced by sodium butyrate. Paper-8302514.
Only total Lp(A-I) triglyceride in women (not men) (r = 0.71, P = 0.009) was significantly correlated with PLTP activity. Paper-9937707.
At baseline, PLTP activity was positively correlated with waist circumference, HbA(1c), glucose, and apoE (all P < 0.05). Paper-11826400.
Firstly, wild type macrophages were injected into wild type, PLTP transgenic (PLTPtg) and apoAI transgenic (apoAItg) mice. Paper-13801514.
It is concluded that human PLTP catalyses exchange/transfer processes of alpha-T between lipid compartments. Paper-155026.
D-myo-inositol 1-phosphate and glycerylphosphorylinositol modestly enhanced PITP-alpha-mediated phospholipid transfer. Paper-10042858.
Multivariate logistic regression analysis revealed that PLTP activity is independently associated with the presence of PAD. Paper-12690156.
Significantly higher PLTP activity levels were measured in seminal plasma samples with low seminal vesicle secretions. Paper-9925447.
In this study, we investigated the effects of lipoproteins on the secretion of PLTP in cultured BeWo choriocarcinoma cells. Paper-10661877.
Phospholipid transfer protein activity is associated with inflammatory markers in patients with cardiovascular disease. Paper-11486757.
The rate of PPyDPC transfer by PLTP decreases with increasing free cholesterol content in rHDL and with decreasing HDL size. Paper-966073.
PLTP activity is elevated in patients with diabetes, a condition with strongly elevated risk for coronary heart disease. Paper-11826400.
Thus, the up-regulatory effect of butyrate on PLTP gene expression seemed to have occurred at the transcriptional level. Paper-8302514.
The rate and extent of mixing of core lipids correlated with the amount of PLTP added and with the increase in particle size. Paper-462626.
Almost all sera recognized specifically the main allergen of apple peal Mal d 3 with molecular weight <10kDa ( LTP). Paper-12721373.
Performing gene hunting in brain of patients with Down syndrome (DS) we detected the absence of a fragment identified as PLTP. Paper-8793444.
Plasma phospholipid transfer protein-mediated reactions are impaired by hypochlorite-modification of high density lipoprotein. Paper-10141915.
RESULTS: We analyzed the PLTP activity and Apo-A1 and Haptoglobin content in six hyperlipidemic and six normolipidemic plasmas. Paper-13944579.
Crystallization and preliminary X-ray crystallographic analysis of phospholipid transfer protein from maize seedlings. Paper-8034349.
Thus, the lower the insulin sensitivity, the lower was HDL cholesterol and the higher were plasma PLTP activity and prebeta-HDL. Paper-8826123.
Phospholipid transfer protein generates small pre beta-HDL particles that are initial acceptors of cell-derived cholesterol. Paper-10086800.
Human studies indicated that PLTP activity positively correlated with aging, obesity, diabetes and coronary artery disease. Paper-9496706.
Phospholipid transfer exhibits saturable first-order kinetics with respect to both cell and vesicle membrane concentrations. Paper-5066794.
Comparison of spontaneous phospholipid transfer between phospholipid vesicles and between phospholipid-bile salt mixed micelles. Paper-6727252.
PLTP mRNA levels in subcutaneous, but not in the visceral, adipose tissue were positively related to the BMI of the subjects. Paper-2054807.
To assess the role of this hydrophobic cluster for the functional activity of PLTP, single point alanine mutants were engineered. Paper-8699386.
CONCLUSION: An elevated PLTP activity in HD patients may be considered as a further aspect of uremic dyslipidemia in HD patients. Paper-13178030.
In the non-obese subjects, HDL(2) cholesterol was found to be significantly and positively related to PLTP activity (r(s) = 0.44). Paper-2171933.
High plasma PLTP activity is associated with insulin resistance in conjunction with altered NEFA and triglyceride metabolism. Paper-1607880.
Phospholipid transfer protein is present in human atherosclerotic lesions and is expressed by macrophages and foam cells. Paper-9937695.
These results suggest that secretion-competent PLTP requires glycosylation but that no single glycosylation site is required. Paper-12151835.
PLTP activity is a risk factor for subsequent cardiovascular events in CAD patients under statin therapy: the AtheroGene Study. Paper-13672804.
Exogenous addition of recombinant PLTP to primary human astrocytes significantly increased apoE secretion to the conditioned medium. Paper-10783843.
Alterations in plasma vitamin E distribution in type 2 diabetic patients with elevated plasma phospholipid transfer protein activity. Paper-10614756.
Lipid transfer protein ( LTP) has been reported as an important allergen inducing severe systemic reactions in allergic subjects. Paper-12721373.
In hyperlipidemic plasma, PLTP activity was found to be increased by Abelcet and AmBisome but not changed by Fungizone. Paper-13983763.
Haplotypes in the phospholipid transfer protein gene are associated with obesity-related phenotypes: the Québec Family Study. Paper-11233524.
Thus, the PLTP gene is highly expressed in alveolar type II epithelial cells and is induced during hypoxia and in emphysema. Paper-1469325.
Moreover, overexpression of PLTP significantly decreased faecal ( 3)H-tracer levels compared with wild type and apoAItg mice. Paper-13801514.
Elevated plasma phospholipid transfer protein activity is a determinant of carotid intima-media thickness in type 2 diabetes mellitus. Paper-10846793.
Whether macrophage PLTP acts at the plasma membrane or intracellularly or shuttles between these compartments needs further study. Paper-12162034.
A total extract of hen egg yolk is used as a simple PL mixture to demonstrate the capabilities of this method. Paper-12581922.
General linear model analysis showed that only apolipoprotein AI, age, BMI, and log(CRP) were independent determinants of PLTP activity. Paper-10802714.
Phospholipid transfer protein enhances removal of cellular cholesterol and phospholipids by high-density lipoprotein apolipoproteins. Paper-1931957.
PLTP contributes to the HDL maturation and increases the ability of HDL to extract the cellular cholesterol. Paper-12516467.
PLTP is synthesized in the liver, and elevated serum transaminases are considered to predict nonalcoholic fatty liver disease (NAFLD). Paper-12752736.
Determination of the label profiles showed that cholesterol as well as phosphatidylcholine were transferred from the vesicles to PLTP. Paper-950295.
Plasma phospholipid transfer protein activity in patients with low HDL and cardiovascular disease treated with simvastatin and niacin. Paper-8946203.
Phospholipid Transfer Protein Sec14 Is Required for Trafficking from Endosomes and Regulates Distinct trans-Golgi Export Pathways. Paper-13653429.
It is suggested that, apart from HDL, plasma PLTP activity is a determinant of cholesterol efflux via stimulation of pre beta-HDL formation. Paper-8998347.
RESULTS: In normolipidemic plasma, PLTP activity was found to be increased by Abelcet and AmBisome but inhibited by Fungizone. Paper-13983763.
Plasma phospholipid transfer protein prevents vascular endothelium dysfunction by delivering alpha-tocopherol to endothelial cells. Paper-1836792.
Crystallization and preliminary X-ray diffraction studies of the Saccharomyces cerevisiae phospholipid-transfer protein Sec14p. Paper-11783290.
Clinical data have recently indicated that plasma PLTP activity and mass were both higher in diabetic patients concomitant with hyperglycemia. Paper-8852119.
Inhibition was highly specific since the activities of phospholipid transfer protein and lecithin cholesterol acyl transferase were not affected. Paper-8522198.
These observations suggest that a hypoxic stimulus occurring in emphysema may be a novel mechanism that contributes to enhanced expression of PLTP. Paper-1469325.
Enzyme-linked immunosorbent assay indicated that seminal PLTP concentrations represented 25% of the concentration measured in blood plasma. Paper-9925447.
Most of these monoclonal antibodies (mAbs) also enhance phospholipid transfer to LDL but in a lesser and variable proportion relative to cholesterol. Paper-204711.
HDL-cholesterol concentrations were substantially increased by fenofibrate in PLTP overexpressing mice (+72%), but unaffected in wild-type mice. Paper-11174394.
Cellular cholesterol efflux to plasma was positively related to pre-beta-HDL and PLTP activity but not significantly to apoM. Paper-13950635.
Plasma phospholipid transfer protein activity is related to insulin resistance: impaired acute lowering by insulin in obese Type II diabetic patients. Paper-1607880.
These kinetic perturbations are probably related to central obesity, insulin resistance, hypertriglyceridemia, and low plasma PLTP activity. Paper-11374918.
The presence of the PLTP antibody resulted in a 20% decrease in the percentage AmpB recovered in the HDL fraction following the incubation of Abelcet. Paper-12110119.
Atorvastatin treatment resulted in decreased PLTP activity (10 mg atorvastatin: -8.3%, P < 0.05; 80 mg atorvastatin: -12.1%, P < 0.002). Paper-11826400.
However, strong coronary risk factors like obesity, diabetes, cigarette smoking and inflammation increase circulating levels of active PLTP. Paper-12690156.
No stabilizing effect of butyrate on PLTP mRNA was apparent upon treatment of the cultured cells with the RNA synthesis inhibitor, actinomycin D. Paper-8302514.
PLTP is distributed widely in the central nervous system (CNS), is synthesized by glia and neurons, and is active in cerebrospinal fluid (CSF). Paper-10783843.
The present study was conducted to determine the essential DNA sequences required for the transcription of the human phospholipid transfer protein gene. Paper-956527.
PLTP helps to enhance the uptake of cell-derived cholesterol by pre beta 1-LpA-I and, thereby, the cholesterol efflux capacity of normal plasma. Paper-575565.
In adherent peripheral blood human macrophages, this PLTP expression was increased by culture with granulocyte macrophage colony-stimulating factor. Paper-9937695.
PLTP is an 80-kDa glycoprotein that is expressed/secreted by a wide variety of tissues including lung, liver, adipose tissue, brain, and muscle. Paper-11290931.
Western blot and phosholipid transfer activity assay demonstrated secretion of active PLTP by neurons, microglia, and astrocytes in culture. Paper-9905027.
Kinetics of phospholipid transfer between liposomes (neutral or negatively charged) and high-density lipoproteins: a spin-label study of early events. Paper-5066817.
Role of phospholipid transfer protein on the plasma distribution of amphotericin B following the incubation of different amphotericin B formulations. Paper-12110119.
The phosphatidylcholine binding affinity and capacity were greater when PLTP was incubated with phosphatidylcholine vesicles without cholesterol. Paper-950295.
Human PLTP transcripts were detected in total RNA from adipose tissue, lung, heart, and spleen of the two distinct lines (A and C) of transgenic mice. Paper-1025887.
Fenofibrate reverses the decline in HDL cholesterol in mice overexpressing human phospholipid transfer protein. Paper-11174394.
High titers of anti- PL to beta2GPI appeared after the second immunization, with T cell reactivity to beta2GPI detectable only after the fourth immunization. Paper-14036171.
In contrast to mammals, PLTP-facilitated HDL remodeling did not enhance cholesterol efflux efficiency of chicken HDL particles. Paper-13894710.
CONCLUSION: These findings indicate that DAGs are probably formed from TG during lipolysis and that they can be transported to HDL through the action of PLTP. Paper-1824150.
PLTP activity was measured at baseline in 46 subjects ( body mass index = 19-64 kg/m2) and after diet-induced weight loss in 19 of the obese subjects. Paper-9973760.
The fraction of phospholipid transfer necessary for a shape change from discoid to two connected vesicles was of the order of 0.1% of the total phospholipids. Paper-7505101.
HRT enhances the plasma capacity to carry out cholesterol efflux from the Fu5AH system and decreases the activity of PLTP, a key protein regulating HDL levels. Paper-9564411.
Importantly, also the large fused HDL particles formed during incubation of HDL with HA- PLTP acted as efficient cholesterol acceptors. Paper-12524026.
Mutagenesis of these PPRE-like sequences, especially that at -322 to -299, abolished most of the reducing effects of fibrate on the PLTP promoter activity. Paper-8320509.
Phospholipid transfer activity of microsomal triacylglycerol transfer protein is sufficient for the assembly and secretion of apolipoprotein B lipoproteins. Paper-11807157.
Human plasma phospholipid transfer protein activity is decreased by acute hyperglycaemia: studies without and with hyperinsulinaemia in Type 1 diabetes mellitus. Paper-11194448.
PLTP mediated equally the transfer of the various headgroup derivatives except phosphatidylethanolamine ( PE), which was transferred 2-3-fold more slowly. Paper-746400.
Use of phospholipid transfer protein as a probe to study the lipid dynamics and alkaline phosphatase activity in the brush border membrane of human term placenta. Paper-12413480.
CONCLUSIONS: APF could be considered as a specific marker against CAD and type 2 diabetes mellitus and our results confirm the atherogenic behavior of PLTP in CAD. Paper-13774518.
Phospholipid transfer protein activity is determined by type 2 diabetes mellitus and metabolic syndrome, and is positively associated with serum transaminases. Paper-12752736.
Whether this is caused by epitopic differences between Rosaceae and bean LTPs or by the fact that LTP is not expressed in bean remains to be established. Paper-11275568.
In insulin resistance, the ability of plasma to promote cellular cholesterol efflux may be maintained consequent to increases in PLTP activity and pre beta-HDL. Paper-10086800.
The molecular and macromolecular specificities of recombinant human PLTP were studied using a fluorometric assay based on the excimer fluorescence of pyrenyl lipids. Paper-966073.
The mechanism of phospholipid transfer between membranes has been studied as a function of the configuration and concentration of donor and recipient membranes. Paper-104911.
Linear regression analysis showed that plasma PLTP activity, triglyceride and age were the major determinants of LDL-III concentration (r2 = 28%, P < 0.001). Paper-9736919.
The multivariate adjusted relative risk of CHD was 0.46 (95% confidence interval, 0.20-1.07) for an increase of 1 standard deviation in the PLTP value (p=0.071). Paper-10305943.
Phosphatidylcholine (PC)16:0/16:0 is highly variable between mammals and is not the major PL in the wombat, dunnart, shrew, or Tasmanian devil. Paper-11000022.
As determined by linear regression analysis, BMI was the sole predictor of phospholipid transfer protein activity in plasma explaining 22.2% of the activity (p< 0.01). Paper-8909767.
RESULTS: Pronounced weight loss after gastric banding surgery resulted in a significant decrease of PLTP activity from 8.42+/-2.04 to 7.43+/-2.21 micromol/ml/h (P=0.02). Paper-10562041.
Role of phospholipid transfer protein and prebeta-high density lipoproteins in maintaining cholesterol efflux from Fu5AH cells to plasma from insulin-resistant subjects. Paper-8826123.
Furthermore, no changes in trafficking were observed when C. trachomatis was grown in a mutant cell line with a nonfunctional, nonspecific phospholipid transfer protein. Paper-1251570.
No correlation between clinical symptoms and sensitization to recombinant apple allergens has been found, LTP may be useful in diagnosis of apple allergy. Paper-12721373.
Plasma phospholipid transfer protein enhances transfer and exchange of phospholipids between very low density lipoproteins and high density lipoproteins during lipolysis. Paper-4985052.
Art v 3, the lipid-transfer protein ( LTP) of Artemisia vulgaris pollen is a relevant allergen showing frequent cross-reactivity with homologues in other plants. Paper-13796292.
We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Paper-4301777.
Under reducing conditions, however, apo-PITP recovered more than 80% of the native transfer activity and was similar to holo-PITP in the kinetics of phospholipid transfer. Paper-1135021.
Such proteolyzed PLTP had reduced ability (i) to transfer PL from liposome donor particles to acceptor HDL(3) particles, and (ii) to facilitate the formation of pre-beta-HDL. Paper-9717548.
To identify the importance of these and the remaining cysteine residues to PLTP secretion and activity, each was replaced by a glycine by site-directed mutagenesis. Paper-1878785.
This indicates that the inhibition was partly due to unspecific effects of the modification on acceptor particle surface properties, but included an aspect specific for PLTP. Paper-1407422.
In this study we clarify the role of the two forms of PLTP in cholesterol efflux from [3H]cholesterol oleate-acetyl-LDL-loaded THP-1 macrophages. Paper-12524026.
PLTP catalysed alpha-T exchange between different lipoprotein classes, as well as the transfer of alpha-T from artificial liposomes to high-density lipoproteins. Paper-155026.
Phospholipid transfer proteins are generally localized in the cytosolic fraction of cells and are capable of catalyzing the flux of phospholipid molecules among membranes. Paper-5183169.
Postprandial variations in the cholesteryl ester transfer protein activity, phospholipid transfer protein activity and plasma cholesterol efflux capacity in normolipidemic men. Paper-9799450.
Efflux to hypertriglyceridemic diabetic plasma is enhanced, in association with increased plasma PLTP activity and cholesterol esterification. Paper-12714755.
Whereas simvastatin significantly increased PLTP activity in an endogenous lipoprotein-dependent assay (P < 0.01), no variation was observed in a lipoprotein-independent assay. Paper-1870732.
The present data indicate that during the acute-phase response, plasma PLTP activity and mass are strongly affected by the lipoprotein distribution as well as lipid composition. Paper-9067669.
Cholesterol efflux from Fu5AH cells to plasma, plasma lipoproteins, PLTP activity and prebeta-HDL formation as measured in incubated plasma were determined after a 12-h fast. Paper-8826123.
Type 2 diabetes mellitus is associated with differential effects on plasma cholesteryl ester transfer protein and phospholipid transfer protein activities and concentrations. Paper-10439136.
The organization of the phospholipid transfer protein gene strikingly resembles that encoding another plasma lipid transfer protein, the human cholesterol ester transfer protein. Paper-165961.
Parallel with the phospholipid-transfer activity modifications in neoplasic cells, changes in the phospholipid composition of microsomes and mitochondria have been observed. Paper-5429879.
In Langmuir monolayer studies high-activity (HA) and low-activity (LA) forms of PLTP associated with fluid phosphatidylcholine monolayers spread at the air/buffer interphase. Paper-13099401.
CONCLUSION: LTP may induce sensitization via the respiratory tract due to inhalation of air-dispersed food particles, and this may precede the onset of food allergy. Paper-12946671.
The reversible nature of the binding was shown by the transfer of labeled cholesterol and phosphatidylcholine bound to PLTP to the acceptor vesicles or low density lipoprotein. Paper-950295.
Hence, in MetS, the effects of fenofibrate on plasma lipid transfer protein activities, especially PLTP activity, may partially explain the associated changes in apoB kinetics. Paper-12172434.
Plasma PLTP activity was determined as the transfer of radiolabelled phosphatidylcholine from small unilamellar phosphatidylcholine vesicles to ultracentrifugally isolated HDL. Paper-12329015.
In vitro studies indicated that PLTP deficiency led to a significant decrease in alpha-tocopherol content and increased oxidative stress in bone marrow cells. Paper-12046556.
One of the deletion mutants (delta 470-475) showed similar reductions in cholesteryl ester and triglyceride transfer activities but normal or increased phospholipid transfer activity. Paper-69273.
We analysed the relationship between carotid artery intima-media thickness (IMT), an established marker of atherosclerosis, and PLTP activity in diabetic patients and control subjects. Paper-10846793.
Plasma HDL-cholesterol and insulin concentrations and activities of cholesterol ester transfer protein and phospholipid transfer protein did not differ significantly between the diets. Paper-10394314.
Phospholipid transfer protein mediates transfer of not only phosphatidylcholine but also cholesterol from phosphatidylcholine-cholesterol vesicles to high density lipoproteins. Paper-950295.
9. The platelet phospholipid transfer protein is able to catalyze the transfer of phosphatidylinositol and phosphatidylcholine between vesicles and human platelet plasma membranes. Paper-5110565.
Cholesterol efflux from macrophage foam cells is enhanced by active phospholipid transfer protein through generation of two types of acceptor particles. Paper-12524026.
To test this, we established a novel assay to monitor phospholipid transfer between the ER and peroxisomes and found that phospholipids are rapidly trafficked between these compartments. Paper-13037294.
Stimulation of phospholipid exchange was confirmed in experiments where PTP was found to augment transfer of [14C]phosphatidylcholine radioactivity from HDL to VLDL during lipolysis. Paper-4985052.
Patients with > 15% stenosis of at least one carotid artery as determined by US underwent intravenous PL (200 mg/kg) or placebo infusions weekly for 8 weeks. Paper-12474367.
Differential display showed the absence of a cDNA fragment and cloning, sequencing and gene bank work revealed 100% homology with human PAC 337018 on chromosome 20q containing the PLTP gene. Paper-8793444.
A fluorescent phospholipid, 1-palmitoyl-2-pyrenedecanoylphosphatidylcholine, was used to study the mechanism of spontaneous phospholipid transfer between single-walled phospholipid vesicles. Paper-3599323.
CONCLUSION: Cellular cholesterol efflux to plasma from patients with nephrotic-range proteinuria is enhanced, in conjunction with elevated pre-beta HDL formation and plasma PLTP activity. Paper-10824608.
Increased phospholipid transfer protein activity associated with the impaired cellular cholesterol efflux in type 2 diabetic subjects with coronary artery disease. Paper-12516467.
A wheat non specific phospholipid transfer protein has been isolated from wheat seeds and its amino acid sequence reveals that it is composed of 90 residues for a molecular weight of 9607. Paper-7401458.
The ATP-dependent lipid transfer was inhibited by N-ethylmaleimide, indicating the involvement of cytosolic (but no phospholipid transfer proteins) or membrane proteins in the transfer process. Paper-7929139.
Investigations have been carried out on phospholipid-transfer activity of the cytosol and the phospholipid composition of subcellular membranes from human liver and primary liver carcinoma. Paper-5429879.
In situ hybridization studies revealed specific, high-level synthesis of PLTP mRNA in choroid plexus and ependyma, the organs responsible for production of cerebrospinal fluid. Paper-9408605.
The lipid transfer protein ( LTP), Pru p 3, has been identified as the major allergen present in peach, and its sequence obtained by direct amino acid sequencing has been previously reported. Paper-13832653.
In this study we have determined the influence of bile salt structure, incorporation of cholesterol, and temperature on the rate and mechanism of phospholipid transfer between mixed micelles. Paper-6671510.
These results show that this phospholipid transfer protein is unique from the human plasma cholesteryl ester transfer protein, and may play an important role in human lipoprotein lipid metabolism. Paper-5927652.
Phosphatidylinositol transfer protein alpha (PITP-alpha) is a bifunctional phospholipid transfer protein that is highly selective for phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho). Paper-10042858.
The non-specific phospholipid transfer protein purified from bovine liver has been used to modify the phospholipid content and phospholipid composition of the membrane of intact human erythrocytes. Paper-4675911.
In this review the major phospholipid transfer proteins are discussed with respect to their phospholipid substrate specificity and the contributions of membrane physical properties to this process. Paper-5183169.
In conclusion, elevated PLTP activity in obese subjects is likely a result of increased body fat, reflected by SQF, and is influenced by NEFAs but is not directly related to insulin resistance. Paper-9973760.
The addition of either the PTP or the d greater than 1.21 g/ml fraction resulted in a 2- to 3-fold stimulation of the transfer of phospholipid radioactivity from VLDL into HDL during lipolysis. Paper-4985052.
In experiments performed with human VLDL and human HDL3, both the d greater than 1.21 g/ml fraction and the PTP were found to stimulate phospholipid mass transfer from VLDL into HDL during lipolysis. Paper-4985052.
Baseline HDL cholesterol and phospholipids, pre beta-HDL in incubated plasma, plasma apolipoprotein (apo) AI, PLTP activity and cholesterol efflux to plasma were not different between the groups. Paper-8998347.
To evaluate how acute-phase HDL (AP-HDL) functions in PLTP-mediated HDL conversion, we collected plasma samples from patients with severe acute-phase response (n=17), and from healthy controls (n=30). Paper-9067669.
Weak associations were also observed between PLTP parameters and determinants of glucose homeostasis ( glucose, insulin, and homeostasis model assessment for insulin resistance). Paper-10610550.
To study the role of these sites on PLTP structure and function, seven variants in which asparagine (N) residues were converted to glycine ( G) were prepared by site-directed mutagenesis. Paper-12151835.
This was confirmed by in vitro studies demonstrating that cholesterol loading of macrophages leads to 2- to 3-fold increases in PLTP steady-state mRNA levels, protein expression, and activity. Paper-9772399.
Although PL liposomes are simple model membranes with sharp transitions which give detailed information about the effects of enhancers, they can provide misleading results. Paper-11233063.
OBJECTIVE: To evaluate the potential role of recombinant Tri a 14 as a novel tool for the diagnosis of baker's asthma, and to test the heat and proteolytic resistance of the wheat LTP allergen. Paper-13894896.
Tangier disease patients, with a mutated ABCA1 transporter, have extremely low plasma HDL concentration and reduced PLTP activity levels, a phenotype that is also observed in mice lacking ABCA1. Paper-13894711.
One anionic PL, phosphatidic acid, shows some quenching interference to both the C14 and C18 dyes but only at concentrations above the working range for sample analysis. Paper-12996584.
The present study was conducted to determine if the promoter activity of the human PLTP gene is affected by fibrate, a hypolipidemic drug, and to identify DNA sequences that are responsible for the effect. Paper-8320509.
Employing thermal analysis, we investigated the mechanism of action of novel enhancers and probed phospholipid ( PL) versus stratum corneum lipid (SCL) liposomes as model membranes. Paper-11233063.
Moreover, chymase effectively depleted the pre-beta-HDL particles generated from HDL(3) by PLTP and significantly inhibited the high affinity component of cholesterol efflux from macrophage foam cells. Paper-9717548.
Decreases in plasma EST and CET in such patients, as well as a low PLTP activity in acromegaly suggest that reverse cholesterol transport may be impaired, contributing to increased cardiovascular risk. Paper-8557360.
Phospholipid transfer protein deficiency, produced by gene knockout in mice, results in decreased HDL levels, reflecting decreased transfer of phospholipids from triglyceride-rich lipoproteins into HDL. Paper-10583703.
Monoclonal immunoaffinity chromatography of plasma or its fractions showed complete removal of cholesteryl ester and triglyceride transfer activities but incomplete removal of phospholipid transfer activity. Paper-5808081.
PLTP mRNA of 1.8 kb was widely distributed in all the examined regions of the central nervous system at either comparable or slightly lower levels than in the other major organs, depending on the region. Paper-9905027.
In the brain, phospholipids in addition are integral constituents of myelins and synaptosomes (Johnson etc) and deficient PLTP levels may account for the deteriorated functions described to occur in DS brain. Paper-8793444.
When the lettuce allergen was incubated with both Pru p 3 from peach peel and recombinant Pru p 3, the immunodetection-inhibition assay indicated that patients were sensitized to the lettuce LTP Lac s 1. Paper-13810095.
The EPA levels in the plasma phospholipid ( PL) fraction were significantly increased after the treatment (from 3.30 +/- 0.64 mol% to 8.01 +/- 0.47 mol%, p < 0.001). Paper-11765952.
The oxidation of HDL3 by Cu(II) and its effect on the ability of these particles to act as phospholipid acceptors in human plasma phospholipid transfer protein (PLTP)-mediated lipid transfer were investigated. Paper-1407422.
CONCLUSION: These findings suggest an inhibitory effect of Haptoglobin over PLTP activity in hyperlipidemic plasma that may contribute to the regulation of reverse cholesterol transport. Paper-13944579.
However, not unexpectedly, there is no correlation between the effects of mAbs on alpha-helicity and their effects on cholesterol or phospholipid transfer since each mAb has a discrete effect on these transfers. Paper-204711.
A partially purified preparation of phospholipid transfer activity was obtained from the d 1.20-1.26 g/ml fraction by a sequence of phenyl-Sepharose, heparin-Sepharose, and carboxymethylcellulose chromatography. Paper-4294510.
Using plasma from healthy subjects, cholesterol efflux was correlated positively with HDL cholesterol, HDL phospholipids, pre beta-HDL in incubated plasma, plasma apo AI and PLTP activity (P<0.05 to P<0.001). Paper-8998347.
Phospholipid transfer from apolipoprotein B containing lipoproteins was increased in heterozygotes when compared with controls (46.66 +/- 23.3 vs. 28.91 +/- 18.05 micromol mL-1 h-1/micromol mL-1 PL, P = 0.05). Paper-9811735.
Our data show that HDL from different chicken models is similar in chemical and physical properties to that of man based on PLTP activity, cholesterol efflux, and HDL conversion assays. Paper-13894710.
Consistent with this interpretation, a membrane-permeable stabilizer of F-actin, jasplakinolide, prevents antigen-stimulated changes in DRM PL composition. Paper-13875132.
No prospective studies have examined the effect of weight loss on PLTP activity and assessed whether the resultant changes in activity are related to changes in body weight, insulin resistance, or both. Paper-9973760.
RESULTS: Plasma total cholesterol (P<0.05), triglycerides (P<0.05), apolipoprotein (apo) A-I (P<0.001), apo B (P<0.001), PLTP activity (P<0.005) and pre-beta HDL formation (P<0.001) were higher in proteinuric patients. Paper-10824608.
Apolipoprotein E could stimulate the phospholipid transfer protein-mediated transfer of surface fragments of triglyceride-rich lipoproteins to high-density lipoprotein, and promote high-density lipoprotein remodelling. Paper-13233644.
The transfer of spin-labeled and fluorescent lipids between sonicated vesicles and different host membranes has been measured in the presence or absence of a phospholipid transfer protein purified from maize seedlings. Paper-32228.
As opposed to previous studies on the structure prediction from the amino acid sequence, the results obtained show that plant non specific phospholipid transfer proteins are not almost entirely composed of beta-sheets. Paper-7401458.
The presence of phospholipid transfer protein in macrophages and atherosclerotic lesions suggests that it could be either anti-atherogenic by facilitating lipid efflux or pro-atherogenic by facilitating lipid retention. Paper-10435001.
CONCLUSIONS: The results of this prospective study indicate that the serum PLTP concentration would serve as a predictor of CHD, independent of HDL cholesterol, triglycerides and other established risk factors. Paper-10305943.
Transmission electron microscopy as well as particle size analysis show that, as a result of PL reactions with Au particles, the initial Au nanoparticle size increases to 5 nm. Paper-11622372.
RESULTS: Urinary nitrogen and vitamin C were higher (P<0.05) during LE, while hepatic levels of vitamin C were higher (P<0.05) with LIP+MVP. Paper-11009312.
Furthermore, recent evidence suggests that phospholipid transfer protein may play a role in reproductive processes, in lipid and lipoprotein metabolism in the central nervous system, and in neurodegenerative disease. Paper-10435001.
We compared the efficacy of MCT/LCT fat emulsions containing a usual (0.12) or a decreased (0.06) ratio of phospholipid to triacylglycerol ( PL:TG) in pediatric patients under surgical stress. Paper-11627857.
Cholesteryl ester and phospholipid transfer activities were determined in plasmas from 14 vertebrates, and lipid transfer values were analyzed in the light of the known atherogenesis susceptibility of studied species. Paper-1621774.
However, modification of a previously established ELISA assay to include a denaturing sample pretreatment with the anionic detergent sodium dodecyl sulphate was required for the detection of the secreted PLTP protein. Paper-9973751.
We propose that phospholipid transfer protein activity becomes reduced under cholestasis conditions because of changes in the chemical composition of high density lipoproteins, such as an increase in phospholipids content. Paper-10904807.
Fluorescent-labeled N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine was used to compare the spontaneous rates of phospholipid transfer between phospholipid vesicles and between phospholipid-bile salt micelles. Paper-6727252.
These studies demonstrate that PLTP plays a major role in facilitating the transfer of phospholipid between lipoproteins, and suggest that triglyceride is a significant modulator of intravascular phospholipid transport. Paper-713179.
This review article presents the state of knowledge on the implication of PLTP in lipoprotein metabolism, on its atherogenic potential, and the complexity of its implication in obesity, insulin resistance and T2DM. Paper-13876335.
HDL cholesterol was positively correlated with the M-value (r=0.65, p< 0.05), whereas plasma PLTP activity (r= -0.59, p <0.05) and prebeta-HDL in incubated plasma (r= -0.66, p<0.05) were negatively correlated with the M-value. Paper-8826123.
When phospholipid transfer protein and vesicles consisting of non-labeled phosphatidylcholine are added the protein catalyzes an exchange of phosphatidylcholine between the labeled donor and non-labeled acceptor vesicles. Paper-3818573.
However, little has been reported concerning the relationships of PLTP with plasma lipoprotein parameters, lipolytic enzymes, body fat distribution, insulin, and glucose in normolipidemic individuals, particularly females. Paper-2171933.
Tacrolimus- versus cyclosporine-treated patients demonstrated a significant increase in femoral neck BMD and PP n-3 PUFA content. Paper-11516413.
Plasma PLTP activity was highly, positively, and selectively correlated with the cholesterol concentration of the buoyant LDL/dense IDL fractions, yet demonstrated a complete absence of an association with the dense LDL fractions. Paper-9603843.
PLTP may be important in vivo in the recycling of PC from mature HDL to nascent HDL, the latter of which are the initial acceptors of cholesterol from peripheral tissue for reverse cholesterol transport to the liver. Paper-966073.
In conclusion, plasma PLTP activity was increased in type 2 diabetic patients with or without CAD, which could impair cellular cholesterol removal and might accelerate atherosclerosis in diabetic patients. Paper-12516467.
Differences in human phospholipid transfer protein activity following incubation of Fungizone compared to lipid-based Amphotericin-B formulations in normolipidemic and hyperlipidemic plasma. Paper-13983763.
To this end, endogenous cholesterol esterification activity, phospholipid transfer activity, and cholesteryl ester transfer activity were measured in total plasma from three analbuminemic patients and five control subjects. Paper-547492.
Comparison of the deduced amino acid sequence with N-terminal sequence data obtained for the intact purified bovine brain protein and rat lung phospholipid transfer protein verified that the cDNAs were PtdIns transfer protein clones. Paper-6213792.
PLTP activity and mass have been reported to be abnormally elevated in type 2 diabetes mellitus (T2DM) and insulin-resistant states, and this elevation is frequently associated with hypertriglyceridemia and obesity. Paper-13876335.
This may be explained, at least in part, by the fact that PLTP as a positive acute phase protein, can accelerate the alterations in (phospho)lipid homeostasis thereby playing a role in the attenuation of the acute phase response. Paper-13445606.
Cholesterol efflux from Fu5AH hepatoma cells induced by plasma of subjects with or without coronary artery disease and non-insulin-dependent diabetes: importance of LpA-I:A-II particles and phospholipid transfer protein. Paper-954075.
Cellular cholesterol efflux tended to be correlated with HDL cholesterol (r=0.55, p < 0.10) as well as with plasma PLTP activity (r=0.56, p<0.10) and was positively correlated with prebeta-HDL in incubated plasma (r=0.74, p<0.01). Paper-8826123.
PLTP activity was positively related to body mass index (P < 0.01), waist to hip circumference ratio (P < 0.001), as well as to fasting blood glucose (P < 0.05) and plasma C-peptide (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) Paper-8201839.
Those alterations in the mole ratios of the protein and the two phospholipids that made the bilayer of the reconstituted vesicles more like the membrane of the endoplasmic reticulum resulted in an increase in phospholipid-transfer rate. Paper-4023807.
These observations demonstrate that only HA- PLTP increases macrophage cholesterol efflux, via formation of efficient cholesterol acceptors, prebeta-HDL and large fused HDL particles. Paper-12524026.
Plasma PLTP activity was higher in obese healthy subjects and obese Type II diabetic patients compared with non-obese healthy subjects (p < 0.05 to 0.01) and was correlated with insulin resistance, plasma TG and NEFA (p = 0.02 to < 0.01). Paper-1607880.
Among non-insulin-dependent diabetes mellitus patients, PLTP levels were positively correlated with fasting glycemia and glycohemoglobin levels (r=0.341, P=0.0220; and r=0.382, P=0.0097, respectively) but not with plasma lipid parameters. Paper-1754515.
Biologically, the downregulation of PLTP maybe involved in the pathology of DS as phospholipids not only are of importance for membrane biogenesis and structure but also in the regulation of cellular metabolism, signaling and growth. Paper-8793444.
Conclusions Plasma PLTP activity may be a significant, independent determinant of LpA-I kinetics in men, and may contribute to the maintenance of the plasma concentration of these lipoprotein particles in setting of hypercatabolism of HDL. Paper-12329015.
PLTP transfers phospholipids and free cholesterol from triglyceride-rich lipoproteins to HDL, phospholipids between HDL particles and facilitates cholesterol efflux from cells. Paper-13893403.
In diabetic patients with or without CAD, PLTP activity was consistently increased, compared to controls, while cellular cholesterol efflux activity was decreased by 20% (p < 0.001) or 13.5% (p < 0.01), respectively. Paper-12516467.
PLTP activity and apoE were measured in patients with type 2 diabetes from the DALI (Diabetes Atorvastatin Lipid Intervention) Study, a 30-week randomized double-blind placebo-controlled trial with atorvastatin (10 and 80 mg daily). Paper-11826400.
We now characterize the signaling requirements and time course for this change, which is manifested as an increase in the recovery of polyunsaturated PL in DRM, particularly in phosphatidylinositol species. Paper-13875132.
CONCLUSIONS: Short-term Acipimox administration impairs the ability of plasma from Type 2 diabetic patients and healthy subjects to stimulate cellular cholesterol efflux, in conjunction with alterations in HDL parameters and in PLTP activity. Paper-9031508.
The results indicated that the promoter activity of the PLTP gene was significantly reduced by fenofibrate, and the area that was mainly responsive to the reducing effect by fibrate was located between -377 and -230 of the 5'-flanking region. Paper-8320509.
The effects of membrane proximity on phospholipid transfer were examined in dilution experiments employing intact erythrocytes, resealed ghosts, erythrocyte membrane buds, and sonicated vesicles as both donor and recipient membranes. Paper-104911.
PLTP-stimulated lipid efflux was absent in Tangier disease fibroblasts, induced by cholesterol loading, and inhibited by brefeldin A treatment, indicating selectivity for the apolipoprotein-mediated lipid removal pathway. Paper-1931957.
This finding shows that the PI:PIRS docking complex can modulate the polymorphic phase transitions in PL membranes, a finding that may help us better understand how glycosyl carrier-linked sugar chains may traverse membranes. Paper-10766426.
Heat denaturation profiles and simulated gastrointestinal digestion assays indicated that Tri a 14 displayed a high heat and digestive proteolytic resistance, comparable to those of peach Pru p 3, the model food allergen of the LTP family. Paper-13894896.
Plasma factor VII activity was strongly negatively correlated with prothrombin time (PT) (Std. Coeff. -0.550), and significantly positively correlated with plasma phospholipid ( PL) stearic acid (Std. Coeff. 0.285). Paper-11151866.
Amino acid sequence of a non-specific wheat phospholipid transfer protein and its conformation as revealed by infrared and Raman spectroscopy. Role of disulfide bridges and phospholipids in the stabilization of the alpha-helix structure. Paper-7401458.
We examined the interrelationships between insulin resistance, the ability of plasma to stimulate cellular cholesterol efflux, HDL cholesterol, plasma PLTP activity and prebeta-HDL in 12 non-diabetic, non-smoking, normotriglyceridaemic men. Paper-8826123.
The aim of the present, cross-sectional study was to analyze the relationship of PLTP to peripheral arterial disease, a marker of generalized atherosclerosis, independently of potentially confounding factors like obesity, diabetes and smoking. Paper-12690156.
Arachidonic acid ( AA) levels in the plasma PL fraction were significantly decreased after the treatment (from 9.47 +/- 0.28 mol% to 7.33 +/- 0.43 mol%, p < 0.001). Paper-11765952.
Among the patients, plasma PLTP activity was highly correlated with the percentage of plasma apo A-I in Lp(A-I) (r=0.514, p < 0.001) and with the apo A-I, phospholipid and cholesterol concentration of Lp(A-I) (r=0.499, 0.478, 0.457, respectively, p < 0.001). Paper-1765233.
CONCLUSIONS: Our data show that lipolysis of TGRLPs and their remodelling by PLTP interact to disturb cellular phospholipid flux and intracellular signaling processes, ultimately leading to apoptosis in human macrophages and endothelial cells. Paper-12465831.
Considering the number and the relevance of candidate genes surrounding the PLTP locus and the absence of missense polymorphisms in the coding region, the associations could be mediated by a second gene allele in linkage disequilibrium with the marker locus. Paper-11233524.
Multiple linear regression analysis demonstrated that cellular cholesterol efflux to plasma was positively and independently related to pre beta-HDL formation, PLTP activity and EST (multiple r=0.48), but not to the diabetic state. Paper-12714755.
Liposomes were prepared by dispersing freeze-dried PL/ CPT mixtures in 25 mM phosphate buffered saline (PBS) of varying pH (5.0-7.8) and CPT concentrations (0, 3 and 6 mM). Paper-11270982.
Plasma PLTP concentration was positively correlated with HDL-cholesterol (r = 0.72; P: <0.001), apolipoprotein (apo) A-I (r = 0.62; P: <0.001) and HDL(2)-cholesterol (r = 0.72; P: <0.001), and was negatively correlated with triacylglycerol (r = -0.45; P: <0. 001). Paper-8432389.
Multiple linear regression analysis showed significant associations between PLTP and HDL cholesterol, triglycerides, low-density lipoprotein cholesterol, and body mass index (standardized beta=0.395, -0.191, -0.064, and -0.064, respectively; R(2)=0.31). Paper-10305943.
The present data, which are the first to be obtained with a phospholipid-transfer protein from a photosynthetic tissue, are compared to the amino acid sequences determined with plant and animal proteins involved in the intracellular transport of hydrophobic compounds. Paper-5635466.
Palmitate pre-treatment further increased oleate uptake, both under basal conditions and in the presence of insulin, with a marked increase in the phospholipid ( PL) fraction, with a concomitant reduction in oleate oxidation. Paper-10775724.
These PLTP-mediated processes are physiologically important in the transfer of surface remnants from lipolyzed triglyceride-rich lipoproteins to nascent HDL particles and in the generation of prebeta-HDL, the initial acceptor of excess peripheral cell cholesterol. Paper-8562754.
Increased cholesterol efflux from cultured fibroblasts to plasma from hypertriglyceridemic type 2 diabetic patients: roles of pre beta-HDL, phospholipid transfer protein and cholesterol esterification. Paper-12714755.
1-O-Hexadecyl-2-O-pyrenedecanyl-sn-glycero-3-phosphocholine, a non-hydrolyzable fluorescent diether analog of phosphatidylcholine (PC), was synthesized as a probe for studying phospholipid transfer to different lipoprotein classes with potential phospholipase activities. Paper-235797.
Third, to establish a causal connection, the addition of vitamin E or treatment with an inhibitor of intracellular iron-dependent peroxidation, desferrioxamine, abolished the elevation in cellular ROS as well as the defect in apoB secretion from PLTP-null hepatocytes. Paper-10734065.
Both the d greater than 1.21 g/ml fraction and the PTP enhanced the transfer of VLDL phospholipid mass into HDL, but the percentage transfer of phospholipid radioactivity was greater than that of phospholipid mass, suggesting stimulation of both transfer and exchange processes. Paper-4985052.
In contrast, mAbs 4H1 (residues 2-8), 3G10 (residues 96-121), and 5F6 (residues 116-141) have little or no effect on either cholesterol or phospholipid transfer, and the epitopes for these three mAbs have been shown in earlier studies to be structurally and functionally related. Paper-204711.
It appears that in real cells, invaginations of the plasma membrane or budding of organelles could be triggered by a phospholipid transfer from one leaflet to the other caused, for instance, by the aminophospholipid translocase which is present in eukaryotic membranes. Paper-7505101.
PLTP was able to bind and neutralize LPS: incubation of LPS with purified recombinant PLTP (rPLTP) resulted in the inhibition of the ability of LPS to stimulate adhesive responses of neutrophils, and addition of rPLTP to blood inhibited cytokine production in response to LPS. Paper-562794.
AIMS/HYPOTHESIS: Phospholipid transfer protein plays a key role in lipoprotein metabolism by catalysing the transfer of phospholipids from triglyceride-rich lipoproteins to high-density lipoproteins and, also, within the high-density lipoprotein family, from particle to particle. Paper-8909767.
We investigated the association of serum PLTP activity with the incidence of a combined endpoint ( myocardial infarction and cardiovascular death) and its relation to other markers of atherosclerosis in 1,085 patients with angiographically documented coronary artery disease (CAD). Paper-13672804.
After one minute incubation of PLTP-conditioned plasma with [3H]cholesterol-labeled fibroblasts, the amount of radioactive cholesterol taken up by pre beta 1-LpA-I was twice as high as in control plasma whereas the amount of [3H]cholesterol taken up by gamma-LpE remained unchanged. Paper-575565.
CONTEXT: Plasma phospholipid transfer protein mediates the transfer of phospholipids from triglyceride-rich lipoproteins, very low density lipoproteins and low density lipoproteins to high density lipoproteins, a process that is also efficient between high density lipoprotein particles. Paper-10904807.
These preliminary results support the hypothesis that, despite a lower HDL cholesterol, the ability of plasma from insulin-resistant subjects to promote cellular cholesterol efflux is not impaired, as a consequence of a higher plasma PLTP activity and enhanced prebeta-HDL formation. Paper-8826123.
PLTP activity correlated significantly (P<0.001) with body mass index (r = 0.22), serum total cholesterol (r = 0.17), the ratio of HDL-cholesterol/total cholesterol (r = -0.20), triglycerides (r = 0.20), apo A-II (r = 0.20), and gamma glutamyl transferase (r = 0.22) values. Paper-8335515.
Thus, incubation of plasma with phospholipid transfer protein increases the concentration of prebeta1-LpA-I and in parallel increases the cholesterol efflux capacity of plasma indicating that lipid transfer proteins modulate cholesterol efflux by modification of HDL subclass composition. Paper-1585473.
METHODS: In six non-diabetic male patients with nephrotic-range proteinuria and 12 matched healthy men, plasma (apo)lipoproteins, pre-beta HDL formation, PLTP activity as well as the ability of plasma to promote cholesterol efflux out of cultured human skin fibroblasts were determined. Paper-10824608.
These data demonstrate that the action of human PLTP in the presence of human apoA-I results in the formation of a dysfunctional HDL subfraction, which is less efficient in the uptake of cholesterol from cholesterol-laden macrophages. Paper-12613129.
If this way of sensitization were effective in the majority of LTP allergic patients (e.g. by exposure to peaches showing intact fuzz in areas where peaches are grown and directly sold on the market) our findings could explain the strange geographical distribution of this type of food allergy. Paper-12946671.
Sonicated vesicles, containing 14C-labeled phospholipids and 3H-labeled triolein, as donor particles and cross-linked erythrocyte ghosts as acceptor particles were used to measure phospholipid transfer activities in unfertilized oocytes and in developing embryos of the toad Bufo arenarum. Paper-5769604.
In particular, the molecular transfer of phospholipids, unesterified cholesterol, alpha-tocopherol and lipopolysaccharides by phospholipid transfer protein suggests that it might be involved both in lipoprotein metabolism and in antimicrobial defence, resulting in a growing interest in this protein. Paper-1533215.
LDL was incubated with either vesicles of choline plasmalogen or phosphatidylcholine in presence of lipoprotein- deficient serum, or with liposomes of ethanolamine plasmalogen or phosphatidylethanolamine together with the non-specific phospholipid transfer protein isolated from beef liver. Paper-401627.
In this study, we measured the serum capacity that promotes cellular cholesterol efflux and the plasma PLTP activity in type 2 diabetic patients with coronary artery disease (CAD) (n = 35), those without CAD (n = 24), and 35 healthy subjects as a sex- and age-matched control. Paper-12516467.
RESULTS: In diabetic patients, carotid IMT (p=0.02), pulse pressure (p=0.003), plasma PLTP activity (p<0.001), triglycerides (p=0.01), C-reactive protein (p<0.01) and insulin (p<0.001) were higher, whereas HDL cholesterol was lower (p<0.001) than in control subjects. Paper-10846793.
Previous studies have shown that diacylglycerols (DAG) are formed during triglyceride hydrolysis in very low density lipoproteins (VLDL), a process that is accompanied by an elevated phospholipid transfer protein (PLTP)-mediated transfer of phospholipids (PL) from VLDL to high density lipoprotein. Paper-8815773.
INTRODUCTION: A previous study demonstrated the efficacy of a phospholipid ( PL) complexed with a protein (apoAI Milano) in causing 4.6% regression of atheroma volume as assessed by intravascular ultrasonography (IVUS) in a group of 47 patients with carotid atherosclerosis. Paper-12474367.
CONCLUSIONS: Taken together, these findings suggest indirect evidence that PLTP may play an important role in the plasma distribution profile of AmpB following the incubation of Abelcet and may be one of the factors responsible for the preferential association of AmpB with HDL when administered as Abelcet. Paper-12110119.
The fractional synthesis rate of surfactant PL from plasma palmitate was significantly higher than that from palmitate synthesized de novo from acetate, and these two sources of palmitate together accounted for only half of the total surfactant production in preterm infants. Paper-10970818.
The recent determination of the phospholipid transfer protein complementary DNA sequence as well as the further characterization of its gene structure will direct future studies toward the understanding of its structure-function correlations, physiological regulation, and clinical assessment at the molecular level. Paper-699816.
These findings suggest that PLTP promotes cell-surface binding and remodeling of HDL so as to improve its ability to remove cholesterol and phospholipids by the apolipoprotein-mediated pathway, a process that may play an important role in enhancing flux of excess cholesterol from tissues and retarding atherogenesis. Paper-1931957.
In ex vivo studies on rabbit aortic segments, the impairment of the endothelium-dependent arterial relaxation induced by oxidized LDL was found to be counteracted by a pretreatment with purified PLTP and alpha-tocopherol-albumin complexes, and both the maximal response and the sensitivity to acetylcholine were significantly improved. Paper-1836792.
Not only plasma PLTP activity (P = 0.001 for diabetic patients; P = 0.01 for healthy subjects), but also pre-beta HDL in incubated plasma (P = 0.001 for diabetic patients; P = 0.03 for healthy subjects) and cellular cholesterol efflux to plasma (P = 0.04 for diabetic patients; P = 0.005 for healthy subjects) were lowered by Acipimox in both groups. Paper-9031508.
RESULTS: Phospholipid transfer protein activity was associated with BMI (r = 0.46, p < 0.01), body fat mass (r = 0.39, p < 0.01), subcutaneous fat area (r = 0.32, p < 0.01) and plasma leptin concentration (r = 0.24, p < 0.01) but not with insulin sensitivity expressed as the k(s) of the insulin tolerance test (kITT value) (r = -0.14, p = 0.40). Paper-8909767.
To investigate the relationship between polyunsaturated fatty acid (PUFA) and bone metabolism in renal transplant patients, plasma phospholipid ( PP) PUFA levels, biochemical markers of bone turnover and bone mineral density (BMD) were determined in 22 recipients of a first renal allograft at baseline and after a mean 24.4 month follow-up. Paper-11516413.
Plasma PLTP activity (P<0.001), CET (P<0.001), leptin (P=0.003), resistin (P<0.001), high sensitive C-reactive protein (P=0.005), and insulin resistance (HOMA(ir)) (P<0.001) were higher, whereas HDL cholesterol (P<0.001) and plasma adiponectin (P<0.001) were lower in 83 type 2 diabetic patients (32 females) than in 83 sex-matched control subjects. Paper-12422641.
It was the objective of this study to determine the effect of supplementation with high doses (60 g) of flax and fish oils on the blood phospholipid ( PL) fatty acid status, and AA/ EPA ratio of individuals with Attention Deficit Hyperactivity Disorder (ADHD), commonly associated with decreased blood omega-3 fatty acid levels. Paper-11760781.
Unexpectedly, we found that inhibitors of actin polymerization ( cytochalasin D and latrunculin A) cause a similar, but more rapid, change in the PL composition of DRMs in the absence of FcepsilonRI activation, indicating that perturbations in the actin cytoskeleton affect the organization of plasma membrane domains. Paper-13875132.
Hydrolysis of phospholipid ( PL) within camptothecin (CPT)-containing liposomes was studied systematically, after elevated lyso-phosphatidylcholine (LPC)-concentrations in pH 5, CPT-containing liposomes (22.1+/-0.9 mol%) relative to control-liposomes (7.3+/-0.5 mol%) occasionally had been observed after four months storage in fridge. Paper-11270982.
These synonyms are used for gene PLTP (phospholipid transfer protein): Phospholipid transfer protein, Lipid transfer protein II.
These accession numbers are used for gene PLTP: Q53H91 (UNIPROT__AC), CAC36020 (NCBI_GENBANK__AC), B3KUE5 (UNIPROT__AC), AAH19898 (NCBI_GENBANK__AC).
PLTP is a homologue of zgc:100903 (zgc:100903) from Danio rerio.
PLTP is a homologue of PLTP (phospholipid transfer protein) from Bos taurus.
PLTP is a homologue of PLTP (phospholipid transfer protein) from Pan troglodytes.
PLTP is a homologue of PLTP (phospholipid transfer protein) from Gallus gallus.
PLTP is a homologue of PLTP (phospholipid transfer protein) from Canis lupus familiaris.
PLTP is a homologue of Pltp (phospholipid transfer protein) from Mus musculus.
PLTP is a homologue of Pltp (phospholipid transfer protein) from Rattus norvegicus.
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