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Determinants of thyrotrope-specific thyrotropin beta promoter activation. Cooperation of Pit-1 with another factor. Paper-463467. Both proteins appear to be necessary for full promoter activity in thyrotropes.
This region interacts with the pituitary-specific transcription factor Pit-1. Paper-463467.
We have recently reported that the proximal region from -133 to -100 (P1) is required for promoter expression in thyrotropes.
TSH beta promoter activity in thyrotropes is conferred by sequences between -270 and -80 of the 5'-flanking region. Paper-463467.
Southwestern analysis with the proximal segment of the P1 region (-117 to -88) reveals interaction with a 50-kDa protein. Paper-463467.
Each protein is able to independently interact with the TSH beta promoter, but neither alone can maintain promoter activity. Paper-463467.
Interestingly, this protein is not found in the pituitary-derived GH3 cells and may represent a thyrotrope-specific transcription factor.
We have utilized the thyrotrope-derived TtT-97 thyrotropic tumors to investigate tissue-specific expression of the TSH beta promoter. Paper-463467.
Thyrotropin (TSH) beta is a subunit of TSH, the expression of which is limited to the thyrotrope cells of the anterior pituitary gland. Paper-463467.
We have found that Pit-1 interacts with the distal portion of the P1 region, and a second protein interacts with the proximal segment of this region. Paper-463467.
Further characterization of this newly identified DNA-binding protein will further our understanding of the tissue-specific expression of the TSH beta gene. Paper-463467.
In this report, we have generated a series of promoter mutations in the P1 region to identify additional protein-DNA interactions and determine their functional significance.
While Pit-1 appears necessary for TSH beta promoter activity in thyrotropes, this transcription factor is not alone sufficient for promoter activity in pituitary-derived cells. Paper-463467.
Important links !
iHOP - Information Hyperlinked over Proteins .
Concept & Implementation by Robert Hoffmann.