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Gastrinoma in vitro: morphological and physiological studies of primary cell cultures.Culture medium contained component I, and gastrin 34, 17, and 14. Paper-6569337.
Cultures exhibited limited growth but viability remained high for 2-3 wk.
Functional gastrin-containing tumor cells were maintained for up to 8 wk without fibroblastoid cell overgrowth. Paper-6569337.
With time the major C-terminal gastrin species in medium changed from gastrin 17 at 3 days to gastrin 34 at 5 wk. Paper-6569337.
Extracts of cultured cells contained gastrin 34, 17, and 14; gastrin 17 was the major form detected at all times. Paper-6569337.
Short-term cultures consisted mainly of colonies composed of small polygonal cells, 70%-90% of which stained positive for immunoreactive gastrin. Paper-6569337.
Secretin, bombesin, gastrin-releasing peptide, L-tryptophan, and ethylamine stimulated gastrin release in a dose-dependent fashion. Paper-6569337.
Cultured cells demonstrated de novo gastrin synthesis, evidenced by their ability to incorporate radiolabeled amino acids into immunoadsorbable gastrinlike material. Paper-6569337.
Somatostatin 14 inhibited secretin, bombesin, and gastrin-releasing peptide stimulated gastrin release but did not alter basal release. Paper-6569337.
Secretin, gastrin-releasing peptide, 8-bromoadenosine 3':5'-cyclic monophosphate, and phorbol, 12-myristate, 13-acetate stimulated the release of gastrin from cultured cells in a time-dependent fashion. Paper-6569337.
Ultrastructurally, cultured tumor cells retained morphological integrity for several weeks; however, with time changes in the appearance of the secretory granules accompanied by evidence of cellular retrodifferentiation were gradually observed.
Primary cultures of gastrin-containing tumor cells free from stromal contamination offer unique advantages for studies of factors that regulate the synthesis and secretion of gastrin and may prove of potential value for studies on cell differentiation and growth. Paper-6569337.
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